WO2005059149A3 - Improved protein production - Google Patents

Improved protein production Download PDF

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Publication number
WO2005059149A3
WO2005059149A3 PCT/EP2004/053378 EP2004053378W WO2005059149A3 WO 2005059149 A3 WO2005059149 A3 WO 2005059149A3 EP 2004053378 W EP2004053378 W EP 2004053378W WO 2005059149 A3 WO2005059149 A3 WO 2005059149A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression
opener
sequence
unit comprises
transcription
Prior art date
Application number
PCT/EP2004/053378
Other languages
French (fr)
Other versions
WO2005059149A2 (en
Inventor
Arie Pieter Otte
Theodorus Hendrikus Jaco Kwaks
Richard George Antonius Sewalt
Blokland Henricus Johannes Van
Abraham Bout
Original Assignee
Chromagenics Bv
Crucell Holland Bv
Arie Pieter Otte
Theodorus Hendrikus Jaco Kwaks
Richard George Antonius Sewalt
Blokland Henricus Johannes Van
Abraham Bout
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics Bv, Crucell Holland Bv, Arie Pieter Otte, Theodorus Hendrikus Jaco Kwaks, Richard George Antonius Sewalt, Blokland Henricus Johannes Van, Abraham Bout filed Critical Chromagenics Bv
Publication of WO2005059149A2 publication Critical patent/WO2005059149A2/en
Publication of WO2005059149A3 publication Critical patent/WO2005059149A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/48—Vector systems having a special element relevant for transcription regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides E1-immortalized retina cells comprising a recombinant protein expression unit which unit comprises a promoter functionally linked to an open reading frame encoding at least one protein of interest, characterized in that said expression unit comprises at least one element improving expression, wherein said element improving expression is chosen from the group consisting of: a) a stabilizing anti-repressor (STAR) sequence; b) a Transcription Pause (TRAP) sequence; and c) a binding site for a member of a chromatin modification system for rendering chromatin more accessible for transcription (opener), wherein said opener is present in said cell. Methods for producing at least one recombinant rotein in such cells are also provided.
PCT/EP2004/053378 2003-12-12 2004-12-09 Improved protein production WO2005059149A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EPPCT/EP03/50999 2003-12-12
EP0350999 2003-12-12
EP0351038 2003-12-17
EPPCT/EP03/51038 2003-12-17

Publications (2)

Publication Number Publication Date
WO2005059149A2 WO2005059149A2 (en) 2005-06-30
WO2005059149A3 true WO2005059149A3 (en) 2006-05-26

Family

ID=34702159

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/053378 WO2005059149A2 (en) 2003-12-12 2004-12-09 Improved protein production

Country Status (1)

Country Link
WO (1) WO2005059149A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2123772A1 (en) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-lactam antibiotic producing strains
CN102131921A (en) 2008-08-05 2011-07-20 帝斯曼知识产权资产管理有限公司 Adipoyl-7-adca producing strains
BRPI1015536A2 (en) 2009-04-24 2019-07-02 Dsm Ip Assets Bv carbohydrate degradation polypeptide and uses thereof
AU2011214327B2 (en) 2010-02-11 2014-09-18 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
MX2012014990A (en) 2010-06-29 2013-02-26 Dsm Ip Assets Bv Polypeptide having swollenin activity and uses thereof.
BR112012033714A2 (en) 2010-06-29 2016-02-16 Dsm Ip Assets Bv polypeptide with acetyl xylan esterase activity and its uses.
DK2588492T3 (en) 2010-06-29 2016-06-27 Dsm Ip Assets Bv POLYPEPTIDE WITH BETA-glucosidase AND USES THEREOF
CN103119156A (en) 2010-06-29 2013-05-22 帝斯曼知识产权资产管理有限公司 Polypeptide having carbohydrate degrading activity and uses thereof
PL2588494T3 (en) 2010-06-29 2018-08-31 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
EA201300060A1 (en) 2010-06-29 2013-05-30 ДСМ АйПи АССЕТС Б.В. POLYPEPTIDE, OWNED OR CONTRIBUTING TO THE ACTIVITY OF CARBON MATERIAL DEGRADATION AND ITS APPLICATION
CA2900140C (en) 2013-02-04 2022-05-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
DK3469076T3 (en) 2016-06-10 2020-11-16 Dsm Ip Assets Bv MUTANT LIPASE AND ITS USE
US20230080079A1 (en) 2018-05-18 2023-03-16 Dsm Ip Assets B.V. Mutant lipase and use thereof
EP3794115A2 (en) 2018-05-18 2021-03-24 DSM IP Assets B.V. Mutant lipase and use thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000063403A2 (en) * 1999-04-15 2000-10-26 Crucell Holland B.V. Recombinant protein production in a human cell using sequences encoding adenovirus e1 protein
WO2003004704A2 (en) * 2001-07-04 2003-01-16 Chromagenics B.V. Method of selecting dna sequence with transcription modulating activity using a vector comprising an element with a gene transcription repressing activity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000063403A2 (en) * 1999-04-15 2000-10-26 Crucell Holland B.V. Recombinant protein production in a human cell using sequences encoding adenovirus e1 protein
WO2003004704A2 (en) * 2001-07-04 2003-01-16 Chromagenics B.V. Method of selecting dna sequence with transcription modulating activity using a vector comprising an element with a gene transcription repressing activity

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PFARR D S ET AL: "DIFFERENTIAL EFFECTS OF POLYADENYLATION REGIONS ON GENE EXPRESSION IN MAMMALIAN CELLS", DNA, MARY ANN LIEBERT, NEW YORK, NY, US, vol. 5, no. 2, 1986, pages 115 - 122, XP002910674, ISSN: 1044-5498 *

Also Published As

Publication number Publication date
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