WO2005038427A3 - Rapid identification of viruses of the upper respiratory tract infection including sars causing coronus viruses - Google Patents

Rapid identification of viruses of the upper respiratory tract infection including sars causing coronus viruses Download PDF

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Publication number
WO2005038427A3
WO2005038427A3 PCT/US2004/017767 US2004017767W WO2005038427A3 WO 2005038427 A3 WO2005038427 A3 WO 2005038427A3 US 2004017767 W US2004017767 W US 2004017767W WO 2005038427 A3 WO2005038427 A3 WO 2005038427A3
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WO
WIPO (PCT)
Prior art keywords
viruses
sequence
coronus
appropriate
causing
Prior art date
Application number
PCT/US2004/017767
Other languages
French (fr)
Other versions
WO2005038427A2 (en
Inventor
Joseph Wing On Tam
Hok Sin Woo
Wing Sze Lo
Sin Fun Sia
Tsz Kam Fang
Original Assignee
Medical Gene Ct Ltd
Joseph Wing On Tam
Hok Sin Woo
Wing Sze Lo
Sin Fun Sia
Tsz Kam Fang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Gene Ct Ltd, Joseph Wing On Tam, Hok Sin Woo, Wing Sze Lo, Sin Fun Sia, Tsz Kam Fang filed Critical Medical Gene Ct Ltd
Publication of WO2005038427A2 publication Critical patent/WO2005038427A2/en
Publication of WO2005038427A3 publication Critical patent/WO2005038427A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

This invention provides a method for the detection of a pathogen through analysis of nucleic acid sequences, comprising the steps of: selecting at least one appropriate target sequence from the pathogen; screening said sequence to obtain at least one appropriate primer for amplification; selecting an appropriate probe from the sequence; immobilizing the allelic-Specific-Oligonucleotide site of each sequence onto a solid matrix suitable for capturing the target sequence; amplifying the sequence(s) wherein the steps (d) or (e) can be performing concurrently or sequentially; performing blot analyses on each sequence; and comparing the results of the blot analysis with using positive or negative controls. This invention further provides a method for amplifying the target sequence of a pathogen for hybridization comprising the steps of: selecting an appropriate primer pair; performing reverse transcription on the primer pair; and performing the first and second round of PCR in a single tube.
PCT/US2004/017767 2003-06-05 2004-06-04 Rapid identification of viruses of the upper respiratory tract infection including sars causing coronus viruses WO2005038427A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47690703P 2003-06-05 2003-06-05
US60/476,907 2003-06-05

Publications (2)

Publication Number Publication Date
WO2005038427A2 WO2005038427A2 (en) 2005-04-28
WO2005038427A3 true WO2005038427A3 (en) 2005-08-18

Family

ID=34465051

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/017767 WO2005038427A2 (en) 2003-06-05 2004-06-04 Rapid identification of viruses of the upper respiratory tract infection including sars causing coronus viruses

Country Status (1)

Country Link
WO (1) WO2005038427A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201121210D0 (en) 2011-12-09 2012-01-18 Health Prot Agency Respiratory infection assay
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
EP3068883B1 (en) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
JP6803327B2 (en) 2014-08-06 2020-12-23 ニューゲン テクノロジーズ， インコーポレイテッド Digital measurements from targeted sequencing
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN111378018B (en) * 2020-03-28 2021-01-19 江苏省疾病预防控制中心(江苏省公共卫生研究院) Test strip for detecting novel coronavirus antibody and preparation method and application thereof
CN111378789A (en) * 2020-06-01 2020-07-07 广州凯普医药科技有限公司 Respiratory tract infection pathogen nucleic acid joint detection kit

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6251598B1 (en) * 1998-10-30 2001-06-26 Interleukin Genetics, Inc. Methods for diagnosing sepsis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6251598B1 (en) * 1998-10-30 2001-06-26 Interleukin Genetics, Inc. Methods for diagnosing sepsis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DRONSTEIN C. ET AL.: "Identification of a novel coronavirus in patients with severe respiratory syndrome", NEW ENGLAND J. MED., vol. 348, no. 20, 15 May 2003 (2003-05-15), pages 1967 - 1976, XP001182527 *

Also Published As

Publication number Publication date
WO2005038427A2 (en) 2005-04-28

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