WO2004111634A1 - Dispositif pour realiser une chromatographie sur couche mince - Google Patents

Dispositif pour realiser une chromatographie sur couche mince Download PDF

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Publication number
WO2004111634A1
WO2004111634A1 PCT/DE2003/000635 DE0300635W WO2004111634A1 WO 2004111634 A1 WO2004111634 A1 WO 2004111634A1 DE 0300635 W DE0300635 W DE 0300635W WO 2004111634 A1 WO2004111634 A1 WO 2004111634A1
Authority
WO
WIPO (PCT)
Prior art keywords
thin
layer chromatography
chromatography plate
vertical
frame
Prior art date
Application number
PCT/DE2003/000635
Other languages
German (de)
English (en)
Inventor
Thomas Raul Appel
Jevgenij Raskatov
Jan Rossa
Original Assignee
Thomas Raul Appel
Jevgenij Raskatov
Jan Rossa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE10209159A priority Critical patent/DE10209159B4/de
Application filed by Thomas Raul Appel, Jevgenij Raskatov, Jan Rossa filed Critical Thomas Raul Appel
Priority to PCT/DE2003/000635 priority patent/WO2004111634A1/fr
Priority to AU2003223839A priority patent/AU2003223839A1/en
Publication of WO2004111634A1 publication Critical patent/WO2004111634A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G01N30/94—Development

Definitions

  • the invention relates to a system for improving thin-layer chromatography analysis, in particular for the analysis of chemical substances and molecular components of organisms such as animals, plants, bacteria, fungi and viruses.
  • the thin polarity analysis uses the different polarity of substances / molecules.
  • a stationary separating agent fixed on the thin layer chromatography plate interacts with the dissolved substances to be separated depending on their properties.
  • the substances cover distances of different lengths on the separation layer of the thin layer chromatography plate. After coloring, substances can be determined qualitatively by assigning the running distance and also quantitatively by densitometry.
  • a single or a system of different solvents or solvent mixtures can be used to develop the thin-layer chromatography plates. Multiple development serves to focus the gangs and improve separation.
  • Separated substances can be made visible by spraying with or dipping in a colorant. In In some cases, subsequent even heating is required.
  • a qualitative evaluation is made by comparing the running distance and type of coloring with known substances. Reference values are needed for a quantitative determination. For this, the substance to be analyzed must be at the same concentration in several known concentrations
  • Thin layer chromatography plate can be applied as the sample. Densitometric evaluation, i.e. Comparison of the color intensities between the sample and controls enables a quantitative determination of the amount of substance contained in the sample. Since the intensity of the coloring can vary somewhat for each plate, these reference values must be reapplied to each thin-layer chromatography plate (H. Jork, W. Funk, W. Fischer, H. Wimmer: “Thin-layer chromatography, volume 1a, physical detection methods: basics , Reagenzien I .; VCH Verlagsges., Weilheim-Basel-Cambridge-New York, 1989).
  • Thin-layer chromatography plates for example glass plates coated with the separating agent silica gel, can be used for material separation due to physical interactions (H. Jork, W. Funk, W. Fischer, H. Wimmer: "Thin-layer chromotography, Volume 1a, physical detection methods: basics, reagents I .; VCH Verlagsges., Weilheim-Basel-Cambridge-New York, 1989).
  • US Pat. No. 6,096,205 discloses a thin-layer chromatography system with analysis unit, which uses a multiplicity of supported thin-layer chromatography plates in a multiplicity of chambers and a CCD camera, so that a large number of samples can be examined simultaneously.
  • the system for improving thin-layer chromatography analysis consists of an application template, a development frame, one or more holding sheets and a heating frame, which can be assigned to a thin-layer chromatography plate and one or more vertical chamber (s) with solvent / solvent mixture, in that the application template allows samples and controls to be applied at exact intervals on the thin-layer chromatography plate, the development frame enables safe and easy handling of the thin-layer chromatography plate during the
  • Chromatography and detection processes inside and outside the chamber make it possible for the holding sheets not to let the filter paper, which serves to saturate the atmosphere, slide off the walls of the chamber by holding it on the wall, and the heating frame for error-free heat treatment of the thin-layer chromatography plates outside the chamber without annoying thermal bridges.
  • FIG. 1 shows a schematic illustration of a system according to the invention
  • Fig. 3 a development frame according to Fig. 1
  • Fig. 4 a tie according to Fig. 1 and
  • Fig. 5 a heating frame.
  • the system according to the invention for improving thin-layer chromatography analysis consists, as shown in FIG. 1, of one or more vertical chamber (s) 1, a thin-layer chromatography plate 2 mounted perpendicular to the bottom of the vertical chamber (s) 1, eluent or solvent mixture 3, which (s) the bottom of the vertical chamber (s)
  • a lining with absorbent material 4 which is mounted on the wall (s) of the vertical chamber (s) 1 and extends into the solvent or solvent mixture 3, an application template 5, a development frame 6 , one or more holding sheets 7 for fastening the absorbent material to the wall (s) of the vertical chamber (s) 1, and a heating frame 8, shown in more detail in FIG. 5, the application template 5, the development frame 6 and the heating frame 8 of the thin-layer chromatography plate 2 and the tie 7 of the vertical chamber 1 and the lining with absorbent material 4 can be assigned by the application template 5 an application of samples and controls at exact intervals on the thin layer chromatography plate
  • the support bow 7 holds the lining with absorbent material 4 on the inner wall of the vertical chamber 1 in a non-slip manner and the heating frame 8 holds the thin-layer plate 2 outside the vertical chamber 1 for heat treatment without annoying thermal bridges.
  • the samples and controls are applied to the thin-layer chromatography plate 2 using a pipette, a syringe with a cannula (for example a Hamilton syringe) or a glass capillary, the application template 5 shown in FIG. 1 being designed with precisely defined recesses 51 which are designed as bores can be provided for the cannula or capillary receptacle, has an outer edge 52 as a spacer, so that when the application template 5 is placed on the thin-layer chromatography plate 2, there is a precisely fitting border and parallel spacing thereof, and thus manual application in precisely defined manner Distances is allowed.
  • a pipette a syringe with a cannula (for example a Hamilton syringe) or a glass capillary
  • the application template 5 shown in FIG. 1 being designed with precisely defined recesses 51 which are designed as bores can be provided for the cannula or capillary receptacle, has an outer edge 52 as a spacer, so
  • the application template 5 consists of a transparent material, for example of plastic or glass, and is provided from above with recesses 51 / bores suitable for the Hamilton syringes or glass capillaries at regular intervals.
  • Recesses 51 / bores are additionally tapered on the side facing away from the thin-layer chromatography plate 2 in order to make it easier to insert the syringes / capillaries.
  • the recesses 51 / holes in the application template can be provided with numbers. 2 shows the shape of the recesses 51 / bores, which are conically recessed at the top of the application template 5 and pass through the application template 5 in the form of a channel downwards.
  • FIG. 2 shows the shape of the outer edge 52, which is the distance between the application template 5
  • Thin layer chromatography plate 2 adjusts and the thin layer chromatography plate 2 fits snugly on the side.
  • the vertical chamber (s) 1 is / are for better saturation of the atmosphere with the solvent / solvent mixture 3 with
  • Fig. 4 illustrated (n) tie 7 find use.
  • the purpose of the retaining bow 7 in the vertical chamber 1 is to fix the lining with absorbent material 4 in the vertical chamber 1 on the inner walls thereof.
  • the development frame 6 which serves to hold and hold the thin-layer chromatography plate 2 to facilitate handling inside and outside the vertical chamber (s) 1.
  • the development frame 6 has two supports at the lower end for receiving thin-layer chromatography plates and has a bow-shaped shape at the upper end, so that the development frame 6 can be easily grasped by hand.
  • a holding tab for example made of metal, which tilts those held by the development frame 6
  • the development frame 6 fits exactly into the interior of the vertical chamber 1 and holds the thin-layer chromatography plate 2 mechanically and / or by gravity at an angle of less than forty-five degrees to the vertical. This has the advantage that, in particular if development is to be carried out in succession with several eluents or if the thin-layer chromatography plate 2 is “washed” before development, the thin-layer chromatography plate 2 does not have to be touched with hands or objects when it is converted into different vertical chambers 1
  • Thin-layer chromatography plate 2 in a colorant which can also be located in a vertical chamber 1, for example, ensures that the development frame 6 securely and uniformly immerses the thin-layer chromatography plate 2 in the dye bath.
  • Both the development frame (s) 6 and the retaining arch (s) 7 are made of organic or inorganic materials (for example plastic, glass or metal), the connection of the individual elements from which the development framework 6 or retaining arch 7 are composed Spot welding or other connecting processes can be carried out. Machining of the development frame 6 or retaining bow 7 from solid material or shaping by casting or hardening from the liquid phase are also possible.
  • the heating frame 8 which is shown in one embodiment in FIG. 5, serves for easy handling of the thin-layer chromatography plate 2 for the visualization of latent colorations on the thin-layer chromatography plate 2 by uniform heating while avoiding undesired thermal bridges.
  • heating frame 8 consists of a metal frame, for example steel or aluminum, with six support fingers 81 pointing upwards. It offers a holder corresponding to the dimensions of the thin-layer chromatography plates used (for example 20 ⁇ 10 cm).
  • the six support fingers 81 - four at the corners and two in the middle of the heating frame 8 - allow both 10 x 10 cm and 20 x 10 cm thin-layer chromatography plates to be used.
  • the support fingers 81 limit the space available for the thin-layer chromatography plate 2 and thus prevent it from slipping.
  • the thin-layer chromatography plate 2 itself lies only on small tips on the top of the support fingers 81, so that undesired thermal bridges are reduced to a minimum.
  • heating frame 8 is provided on the support fingers 81 with support points for the thin-layer chromatography plate 2, which can be formed, for example, by milled recesses. These recesses serve to fix the thin-layer chromatography plate 2 and prevent it from slipping when forces act on its side. The thin-layer chromatography plate 2 is therefore only in a punctiform and non-slip manner on the support fingers 81.
  • the shape of the heating part 8 can deviate from the above embodiment, as long as the basic principle of mechanical and / or gravity-related storage of one or two thin-layer chromatography plate (s) 2 on their underside or their top side and / or their corners and / or their outside sides while minimizing heat-conducting bridges is satisfied. LIST OF REFERENCES:

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

L'invention concerne un système pour améliorer l'analyse par chromatographie sur couche mince, notamment de substances chimiques et de constituants moléculaires d'organismes. Le système selon l'invention comprend l'agencement de dispositifs: une ou deux plaques de chromatographie sur couche mince, sur laquelle (lesquelles) des échantillons liquides ou dissous sont déposés manuellement par l'intermédiaire de pipettes, seringues, canules ou capillaires; des chambres de développement verticales contenant des solvants ou des mélanges de solvants; des revêtements pour les chambres verticales mentionnées ci-dessus, contenant un matériau absorbant servant à enrichir l'intérieur des chambres verticales avec des vapeurs de solvants; des arcs de maintien servant à fixer le matériau absorbant sur les parois intérieures des chambres verticales; des supports de développement pour maintenir par voie mécanique et/ou sous l'effet de la gravité des plaques de chromatographie sur couche mince dans une position angulaire inférieure à 45 degrés par rapport à la verticale, lesdits supports étant appropriés pour l'introduction des plaques de chromatographie sur couche mince dans les chambres verticales et pour les plonger dans des liquides; des supports de chauffage servant à maintenir, par voie mécanique et/ou sous l'effet de la gravité, une ou deux plaques de chromatographie sur couche mince, en minimisant les ponts thermiques; une étuve ou un four servant à réaliser un traitement thermique homogène des plaques de chromatographie sur couche mince sur le support de chauffage; des gabarits de dépôt servant à positionner de manière exacte et reproductible les pipettes, seringues, canules ou capillaires lors du dépôt manuel des échantillons.
PCT/DE2003/000635 2002-02-26 2003-04-07 Dispositif pour realiser une chromatographie sur couche mince WO2004111634A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE10209159A DE10209159B4 (de) 2002-02-26 2002-02-26 Anordnung zur Verbesserung der dünnschichtchromatographischen Analyse
PCT/DE2003/000635 WO2004111634A1 (fr) 2002-02-26 2003-04-07 Dispositif pour realiser une chromatographie sur couche mince
AU2003223839A AU2003223839A1 (en) 2003-04-07 2003-04-07 System for thin-layer chromatography

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10209159A DE10209159B4 (de) 2002-02-26 2002-02-26 Anordnung zur Verbesserung der dünnschichtchromatographischen Analyse
PCT/DE2003/000635 WO2004111634A1 (fr) 2002-02-26 2003-04-07 Dispositif pour realiser une chromatographie sur couche mince

Publications (1)

Publication Number Publication Date
WO2004111634A1 true WO2004111634A1 (fr) 2004-12-23

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PCT/DE2003/000635 WO2004111634A1 (fr) 2002-02-26 2003-04-07 Dispositif pour realiser une chromatographie sur couche mince

Country Status (2)

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DE (1) DE10209159B4 (fr)
WO (1) WO2004111634A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2535835A (en) * 2014-12-17 2016-08-31 Micromass Ltd A two-dimensional separation and imaging technique for the rapid analysis of biological samples
US9607816B2 (en) 2014-12-17 2017-03-28 Micromass Uk Limited Two-dimensional separation and imaging technique for the rapid analysis of biological samples

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104833765B (zh) * 2015-05-19 2016-08-03 齐齐哈尔医学院 悬垂薄层色谱仪
CN108459133B (zh) * 2018-04-12 2024-04-23 成都爱恩通医药科技有限公司 薄层色谱分析自动化智能检测仪器及其实时在线检测方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2217595A1 (de) * 1971-04-21 1972-11-02 Continental Oil Co., Ponca City, OkIa. (V.StA.) Wärmeverteilungsplatte und Verfahren zum Brennen von Dünnschichtchromatogrammen
DE2330533A1 (de) * 1973-06-15 1975-01-09 Desaga Gmbh Nachf Erich Fecht Rillenklammer
DE2823476A1 (de) * 1978-03-17 1979-09-20 Artusi & Buncak Scilab Kassettentrennkammer fuer die duennschichtchromatographie
DE7916958U1 (de) * 1979-06-13 1979-09-27 Jenaer Glaswerk Schott & Gen., 6500 Mainz Heizgestell
US4272381A (en) * 1979-10-22 1981-06-09 Schleicher & Schuell, Inc. Thin-layer chromatography spotting
US4351800A (en) * 1981-02-06 1982-09-28 Biochemical Diagnostics, Inc. Thin layer plate chromatography apparatus

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6096205A (en) * 1998-05-13 2000-08-01 The Regents Of The University Of California Hand portable thin-layer chromatography system

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2217595A1 (de) * 1971-04-21 1972-11-02 Continental Oil Co., Ponca City, OkIa. (V.StA.) Wärmeverteilungsplatte und Verfahren zum Brennen von Dünnschichtchromatogrammen
DE2330533A1 (de) * 1973-06-15 1975-01-09 Desaga Gmbh Nachf Erich Fecht Rillenklammer
DE2823476A1 (de) * 1978-03-17 1979-09-20 Artusi & Buncak Scilab Kassettentrennkammer fuer die duennschichtchromatographie
DE7916958U1 (de) * 1979-06-13 1979-09-27 Jenaer Glaswerk Schott & Gen., 6500 Mainz Heizgestell
US4272381A (en) * 1979-10-22 1981-06-09 Schleicher & Schuell, Inc. Thin-layer chromatography spotting
US4351800A (en) * 1981-02-06 1982-09-28 Biochemical Diagnostics, Inc. Thin layer plate chromatography apparatus

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2535835A (en) * 2014-12-17 2016-08-31 Micromass Ltd A two-dimensional separation and imaging technique for the rapid analysis of biological samples
US9607816B2 (en) 2014-12-17 2017-03-28 Micromass Uk Limited Two-dimensional separation and imaging technique for the rapid analysis of biological samples
US10032612B2 (en) 2014-12-17 2018-07-24 Micromass Uk Limited Two-dimensional separation and imaging technique for the rapid analysis of biological samples
GB2535835B (en) * 2014-12-17 2019-09-04 Micromass Ltd A two-dimensional separation and imaging technique for the rapid analysis of biological samples

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Publication number Publication date
DE10209159B4 (de) 2004-06-24
DE10209159A1 (de) 2003-09-11

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