WO2004100833A1 - Procede d'ensemencement de cellules - Google Patents

Procede d'ensemencement de cellules Download PDF

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Publication number
WO2004100833A1
WO2004100833A1 PCT/JP2004/006666 JP2004006666W WO2004100833A1 WO 2004100833 A1 WO2004100833 A1 WO 2004100833A1 JP 2004006666 W JP2004006666 W JP 2004006666W WO 2004100833 A1 WO2004100833 A1 WO 2004100833A1
Authority
WO
WIPO (PCT)
Prior art keywords
cell
tissue
cells
containing solution
flow
Prior art date
Application number
PCT/JP2004/006666
Other languages
English (en)
Japanese (ja)
Inventor
Mitsuo Umezu
Kiyotaka Iwasaki
Shigeyuki Ozaki
Yuji Morimoto
Osamu Endo
Original Assignee
Waseda University
Aubex Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Waseda University, Aubex Corporation filed Critical Waseda University
Publication of WO2004100833A1 publication Critical patent/WO2004100833A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells

Definitions

  • the above-mentioned heterogeneous biological valve can supply a sufficient number, and the patient does not have to continue to drink the anticoagulant for the rest of the life after the transplantation. Expected to be more useful than alternative valves.
  • the present invention has been devised based on such knowledge, and its purpose is to facilitate the attachment of living cells of a transplant destination to a living tissue such as a heterogeneous biological valve, so that a living body after transplantation can be obtained.
  • the object is to provide a cell seeding method capable of enhancing the biocompatibility of a tissue.
  • a cell seeding method of immersing the biological tissue in a cell-containing solution containing the biological cells of the transplant destination and attaching the biological cells to the biological tissue when transplanting a predetermined biological tissue, a cell seeding method of immersing the biological tissue in a cell-containing solution containing the biological cells of the transplant destination and attaching the biological cells to the biological tissue,
  • the adjustment tube 25 is disposed upstream of the resistance applying means 23 and adjusts the amplitude of the pulse pressure of the upstream line L 1. That is, the adjusting tube 25 is formed of a soft material that can adjust the amplitude of the pulse pressure in the upstream line L 1 by changing its wall thickness. For example, the adjusting tube 25 is formed of segmented polyurethane, silicon, or the like. Yes. In this embodiment, the amplitude force of the pulse pressure in the upstream line L 1 is set to be, for example, ⁇ 2 O mmH g of the average pulse pressure (l OO mmH g) so as to approximate the human body. .
  • Support shafts 4 4, 4 4 extending through the cover 3 8 from both the left and right sides of 40 and extending in the directions of the support members 3 6, 3 6, two introduction tubes 4 6 connected to the holding body 3 9 and the discharge tube 1 and 4 and 7.
  • the sub-channel 92 is opened at four positions in the circumferential direction at substantially equal intervals on the inclined surface of the skirt-shaped portion 80.
  • the total opening area obtained by combining the opening areas of the sub-channels 92 is set to be substantially the same as the opening area of the main channel 91. As shown in FIG.
  • the outflow part 63 includes substantially the same components with respect to the inflow part 62, although the parts are different in shape but substantially the same. It has come to play.
  • the both ends of the vascular tissue B are fitted and clamped on the stopper grooves 8 5 and 85 using the binding members not shown.
  • the vascular tissue B is set in a direction allowing the flow of each solution from right to left in FIG. 5 by the aortic valve V which is a one-way valve.
  • the inflow part 62 and the outflow part 63 connected to each other via the vascular tissue B are returned again into the cylindrical member 61, and the end members 6 8 and 6 8 are attached to the cylindrical member 61.
  • the holding body 39 is again attached to the rotating tubes 1 1 2 and 1 1 2 to complete the setting of the vascular tissue B.
  • each insertion tube 7 8, 7 8 in the holding body 39 is rotated from the state of FIG. 5 by loosening the flange portion 97, and then rotating the cylindrical portion 95, The hole 10 5 (see Fig. 5 etc.) and the secondary flow path 92 can be completely blocked.
  • the cell-containing solution is supplied into the insertion tube 78 of the inflow part 62, and enters the vascular tissue B through the tip hole 104 and the main channel 91 of the inflow part 62.
  • vascular tissue B including aortic valve V is collected from animals such as pigs. Then, as described above, the blood vessel tissue B is set in the holding device 10 B, the cell removal solution is injected into the biological tissue processing device 10, and the cell removal solution is circulated.
  • the cell removal solution for example, deoxycholic acid (bile acid), sodium dodecyl sulfate Surfactants such as rum (SDS) and Triton X—100 are used.
  • the cell removal solution circulates in a flow state similar to the blood flow of the human body as follows.
  • Example 2 in addition to the conditions of Example 1, a predetermined microwave was irradiated while rotating the vascular tissue using the holding device 1OB.
  • a microphone mouth wave with a frequency of 2.45 GHz was used, and three combinations of output and irradiation time were performed. That is, when irradiation was performed for 8 hours at an output of 10 OW, irradiation was performed for 12 hours at an output of 50 OW, and irradiation was performed for 24 hours at an output of 50 OW.
  • the rotation speed (rotation speed) of the vascular tissue was 4 rotations per minute. Then, in the same manner as in Example 1, the average number per unit area (1 mm 2 ) of the original cells remaining in the vascular tissue was counted.
  • Ml 99 is mixed with FBS (Fetal Bovine Serum IWK-500, manufactured by Iwaki), antibiotics (penicillin streptomycin mixed solution), and FGF_2 (produced by Pepro Tech E c L td) )
  • FBS Fetal Bovine Serum IWK-500, manufactured by Iwaki
  • antibiotics penicillin streptomycin mixed solution
  • FGF_2 produced by Pepro Tech E c L td
  • Example 3 As a comparative example with respect to Examples 3 to 7 described above, the vascular tissue was kept stationary without rotating up and down with respect to Example 3. Then, as in Example 3 and the like, the state of the autologous cells seeded in the blood vessel tissue was observed.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)

Abstract

Lors de la transplantation d'un tissu d'organisme vivant donné, on prépare une solution contenant des cellules, dans laquelle sont incorporées des cellules d'organisme vivant receveur de la transplantation ; on applique à la solution contenant des cellules un écoulement approximativement égal au flux sanguin du corps vivant receveur de la transplantation ; et le tissu d'organisme vivant est placé dans cet écoulement, afin d'immerger le tissu d'organisme vivant dans la solution contenant les cellules. Lors de cette immersion, on met en rotation le tissu d'organisme vivant, de telle sorte que les directions de révolution des cotés supérieur et inférieur du tissu d'organisme vivant soient inversées l'une par rapport à l'autre. Il en résulte que l'orientation des cellules d'organisme vivant adhérant au tissu d'organisme vivant après l'élimination des cellules peut être améliorée, pour que les cellules de l'organisme vivant puissent être vitalisées. Ainsi, des cellules d'organisme vivant peuvent être fixées au tissu d'organisme vivant avec une densité accrue, ce qui entraîne une amélioration de la biocompatibilité du tissu d'organisme vivant après la transplantation.
PCT/JP2004/006666 2003-05-15 2004-05-12 Procede d'ensemencement de cellules WO2004100833A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003-138016 2003-05-15
JP2003138016A JP2006223317A (ja) 2003-05-15 2003-05-15 細胞播種方法

Publications (1)

Publication Number Publication Date
WO2004100833A1 true WO2004100833A1 (fr) 2004-11-25

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2004/006666 WO2004100833A1 (fr) 2003-05-15 2004-05-12 Procede d'ensemencement de cellules

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JP (1) JP2006223317A (fr)
WO (1) WO2004100833A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2405891B1 (fr) * 2009-03-11 2019-07-03 Exogenesis Corporation Procédé de modification de la mouillabilité et/ou d'autres caractéristiques de biocompatibilité d'une surface d'un matériau biologique par application d'une technologie de faisceau d'ions par amas gazeux et matériaux biologiques ainsi constitués
US9114195B2 (en) 2011-08-22 2015-08-25 Exogenesis Corporation Method for modifying the wettability and other biocompatibility characteristics of a surface of a biological material by the application of beam technology and biological materials made thereby

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11504216A (ja) * 1995-04-27 1999-04-20 アドバンスト ティシュー サイエンシズ，インコーポレーテッド 組織、合成または天然血管移植片の滅菌、接種、培養、保存、輸送ならびに検査を行う装置および方法
JP2001517062A (ja) * 1995-06-07 2001-10-02 アドバンスト ティシュー サイエンシズ，インコーポレーテッド 心臓弁の播種
JP2003116519A (ja) * 2001-10-16 2003-04-22 Yasuhiko Tabata 人工組織用材料

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11504216A (ja) * 1995-04-27 1999-04-20 アドバンスト ティシュー サイエンシズ，インコーポレーテッド 組織、合成または天然血管移植片の滅菌、接種、培養、保存、輸送ならびに検査を行う装置および方法
JP2001517062A (ja) * 1995-06-07 2001-10-02 アドバンスト ティシュー サイエンシズ，インコーポレーテッド 心臓弁の播種
JP2003116519A (ja) * 2001-10-16 2003-04-22 Yasuhiko Tabata 人工組織用材料

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
IWASAKI KIYOTAKA ET AL.: "Daidomyakuben okikaeyo o mezashita buta soshikiben no datasusaiboka shuho no kento oyobi in vitro hakudoka deno saibo hashuyo bioreactor no kaihatsu", THE JAPAN SOCIETY OF MECHANICAL ENGINEERS KANTO SHIBU DAI 9 KI SOKAI KOENKAI KOEN RONBUNSHU, 13 March 2003 (2003-03-13), pages 83 - 84, XP002979700 *

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