WO2004083405A8 - Separation and accumulation of subcellular components, and proteins derived therefrom - Google Patents

Separation and accumulation of subcellular components, and proteins derived therefrom

Info

Publication number
WO2004083405A8
WO2004083405A8 PCT/US2004/008655 US2004008655W WO2004083405A8 WO 2004083405 A8 WO2004083405 A8 WO 2004083405A8 US 2004008655 W US2004008655 W US 2004008655W WO 2004083405 A8 WO2004083405 A8 WO 2004083405A8
Authority
WO
WIPO (PCT)
Prior art keywords
separation
accumulation
methods
subcellular organelles
derived therefrom
Prior art date
Application number
PCT/US2004/008655
Other languages
French (fr)
Other versions
WO2004083405A3 (en
WO2004083405A2 (en
Inventor
Zvi G Loewy
Original Assignee
Alfa Wassermann Inc
Zvi G Loewy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alfa Wassermann Inc, Zvi G Loewy filed Critical Alfa Wassermann Inc
Priority to JP2006507434A priority Critical patent/JP2006520606A/en
Priority to AU2004221348A priority patent/AU2004221348A1/en
Priority to CA002518555A priority patent/CA2518555A1/en
Priority to EP04757676A priority patent/EP1608969A4/en
Publication of WO2004083405A2 publication Critical patent/WO2004083405A2/en
Publication of WO2004083405A3 publication Critical patent/WO2004083405A3/en
Publication of WO2004083405A8 publication Critical patent/WO2004083405A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

The present invention provides for methods for proteome fractionation through the separation and accumulation of subcellular organelles from a biological sample such that the subcellular organelles are highly enriched, substantially pure, and whose structural integrity and functions are well-preserved. The methods of the invention provide a manner by which to reduce the complexity of the proteome and facilitate the detection and isolation of difficult-to-study proteins, such as low-abundance proteins. The methods of the present invention for pre-fractionating proteomes of biological samples by parallel separation and isolation of subcellular organelles from the biological samples using continuous-flow ultracentrifugation are also easily and effectively scalable through adjustment to ultracentrifugation parameters, such as, for example, rotor speed, rotor size, rotor geometry.
PCT/US2004/008655 2003-03-19 2004-03-19 Separation and accumulation of subcellular components, and proteins derived therefrom WO2004083405A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2006507434A JP2006520606A (en) 2003-03-19 2004-03-19 Separation and accumulation of intracellular components and proteins derived therefrom
AU2004221348A AU2004221348A1 (en) 2003-03-19 2004-03-19 Separation and accumulation of subcellular components, and proteins derived therefrom
CA002518555A CA2518555A1 (en) 2003-03-19 2004-03-19 Separation and accumulation of subcellar components, and proteins derived therefrom
EP04757676A EP1608969A4 (en) 2003-03-19 2004-03-19 Separation and accumulation of subcellular components, and proteins derived therefrom

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US45576703P 2003-03-19 2003-03-19
US60/455,767 2003-03-19
US74131303A 2003-12-19 2003-12-19
US10/741,313 2003-12-19

Publications (3)

Publication Number Publication Date
WO2004083405A2 WO2004083405A2 (en) 2004-09-30
WO2004083405A3 WO2004083405A3 (en) 2005-04-07
WO2004083405A8 true WO2004083405A8 (en) 2005-11-17

Family

ID=33032702

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/008655 WO2004083405A2 (en) 2003-03-19 2004-03-19 Separation and accumulation of subcellular components, and proteins derived therefrom

Country Status (6)

Country Link
EP (1) EP1608969A4 (en)
JP (1) JP2006520606A (en)
KR (1) KR20050119128A (en)
AU (1) AU2004221348A1 (en)
CA (1) CA2518555A1 (en)
WO (1) WO2004083405A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004022580A2 (en) 2002-09-09 2004-03-18 Dana-Farber Cancer Institute, Inc. Bh3 peptides and method of use thereof
CA2645853A1 (en) 2006-03-31 2007-11-01 Dana-Farber Cancer Institute Methods of determining cellular chemosensitivity
US9234875B2 (en) 2011-11-04 2016-01-12 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
CN104254390B (en) 2012-02-15 2017-05-10 生物辐射实验室股份有限公司 electronic control of pH and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
WO2014047342A1 (en) 2012-09-19 2014-03-27 Dana-Farber Cancer Institute, Inc. Dynamic bh3 profiling
CA2922503C (en) 2013-09-19 2021-10-26 Dana-Farber Cancer Institute, Inc. Methods of bh3 profiling
CN107428799A (en) * 2015-01-13 2017-12-01 阿尔法韦士曼公司 Purify adeno-associated virus (AAV) and/or the method and its gradient and flow type buffer solution of recombinant adeno-associated virus (rAAV)
EP3288964B1 (en) * 2015-04-27 2024-02-21 Dana-Farber Cancer Institute, Inc. High throughput bh3 profiling: a rapid and scalable technology to bh3 profile on low numbers of cells
KR20160133837A (en) 2015-05-13 2016-11-23 고려대학교 산학협력단 Apparatus for separating fine endoplasmic reticulum by electrophoresis sample pH adjustment
KR20160133826A (en) 2015-05-13 2016-11-23 고려대학교 산학협력단 Apparatus for separating fine endoplasmic reticulum using electrophoresis
CA3048844C (en) * 2016-12-29 2023-12-12 Shoreline Biome, Llc Combined lysis protocol for comprehensive cell lysis
US11149246B2 (en) 2016-12-29 2021-10-19 Shoreline Biome, Llc Methods for cell lysis and preparation of high molecular weight DNA from modified cells
WO2021215878A1 (en) 2020-04-24 2021-10-28 고려대학교 산학협력단 Microvesicle isolation method and microvesicle isolation device
CA3234817A1 (en) * 2021-10-12 2023-04-20 Remo MOOMIAIE Systems and methods for manufacturing of therapeutic cells

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4217418A (en) * 1978-05-08 1980-08-12 Merck & Co., Inc. Recovery of small particles by flow centrifugation
DE69332926T2 (en) * 1993-05-28 2004-03-11 Baxter International Inc., Deerfield CONTINUOUS CENTRIFUGATION METHOD FOR SEPARATING BIOLOGICAL COMPONENTS FROM HETEROGENIC CELL POPULATIONS
AU707878B2 (en) * 1995-12-11 1999-07-22 Dendreon Corporation Cell separation composition, kit and method
US5989835A (en) * 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US20020115056A1 (en) * 2000-12-26 2002-08-22 Goodlett David R. Rapid and quantitative proteome analysis and related methods
DE10158860B4 (en) * 2001-11-30 2007-04-05 Bruker Daltonik Gmbh Mass spectrometric protein mixture analysis
US20040107057A1 (en) * 2002-03-22 2004-06-03 Capaldi Roderick A Enhanced protein separation and analysis

Also Published As

Publication number Publication date
KR20050119128A (en) 2005-12-20
WO2004083405A3 (en) 2005-04-07
EP1608969A4 (en) 2007-06-06
CA2518555A1 (en) 2004-09-30
WO2004083405A2 (en) 2004-09-30
EP1608969A2 (en) 2005-12-28
JP2006520606A (en) 2006-09-14
AU2004221348A1 (en) 2004-09-30

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