WO2004072624A1 - 蛍光相関分光解析装置 - Google Patents

蛍光相関分光解析装置 Download PDF

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Publication number
WO2004072624A1
WO2004072624A1 PCT/JP2004/001558 JP2004001558W WO2004072624A1 WO 2004072624 A1 WO2004072624 A1 WO 2004072624A1 JP 2004001558 W JP2004001558 W JP 2004001558W WO 2004072624 A1 WO2004072624 A1 WO 2004072624A1
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WO
WIPO (PCT)
Prior art keywords
fluorescent
analysis device
correlated
optical system
ccd camera
Prior art date
Application number
PCT/JP2004/001558
Other languages
English (en)
French (fr)
Inventor
Hirohiko Watanabe
Motoyuki Watanabe
Takayuki Inoue
Original Assignee
Hamamatsu Photonics K.K.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hamamatsu Photonics K.K. filed Critical Hamamatsu Photonics K.K.
Priority to JP2005505002A priority Critical patent/JP4495083B2/ja
Priority to EP04710981A priority patent/EP1593957B1/en
Priority to DE602004018272T priority patent/DE602004018272D1/de
Priority to US10/545,393 priority patent/US7400396B2/en
Publication of WO2004072624A1 publication Critical patent/WO2004072624A1/ja
Priority to US12/000,732 priority patent/US7737389B2/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2803—Investigating the spectrum using photoelectric array detector
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2889—Rapid scan spectrometers; Time resolved spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N25/00—Circuitry of solid-state image sensors [SSIS]; Control thereof
    • H04N25/40—Extracting pixel data from image sensors by controlling scanning circuits, e.g. by modifying the number of pixels sampled or to be sampled
    • H04N25/44—Extracting pixel data from image sensors by controlling scanning circuits, e.g. by modifying the number of pixels sampled or to be sampled by partially reading an SSIS array
    • H04N25/443—Extracting pixel data from image sensors by controlling scanning circuits, e.g. by modifying the number of pixels sampled or to be sampled by partially reading an SSIS array by reading pixels from selected 2D regions of the array, e.g. for windowing or digital zooming
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2803—Investigating the spectrum using photoelectric array detector
    • G01J2003/2813—2D-array
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2803—Investigating the spectrum using photoelectric array detector
    • G01J2003/282—Modified CCD or like
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6423—Spectral mapping, video display
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N25/00—Circuitry of solid-state image sensors [SSIS]; Control thereof
    • H04N25/70—SSIS architectures; Circuits associated therewith
    • H04N25/71—Charge-coupled device [CCD] sensors; Charge-transfer registers specially adapted for CCD sensors

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  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Multimedia (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

蛍光相関分光解析装置１は、励起光照射光学系２１、蛍光結像光学系２２、ＣＣＤカメラ１５、及びデータ解析装置１６を備えている。励起光照射光学系２１は、被測定試料Ｓの所定領域に励起光を照射する。蛍光結像光学系２２は、被測定試料Ｓで発生した蛍光をＣＣＤカメラ１５の光検出面に結像する。ＣＣＤカメラ１５は、光検出面に入射した蛍光を各画素毎に光電変換し、光電変換により発生した電荷を出力端から検出信号として出力する。データ解析装置１６は、ＣＣＤカメラ１５の画素のうち解析対象画素群に属する画素において発生した電荷による検出信号を入力し、入力した検出信号の自己相関関数を各画素毎に演算する。これにより、被測定試料の多点に対して同時且つ高速に蛍光相関分光解析を行うことが可能な蛍光相関分光解析装置が得られる。
PCT/JP2004/001558 2003-02-13 2004-02-13 蛍光相関分光解析装置 WO2004072624A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2005505002A JP4495083B2 (ja) 2003-02-13 2004-02-13 蛍光相関分光解析装置
EP04710981A EP1593957B1 (en) 2003-02-13 2004-02-13 Fluorescent correlated spectrometric analysis device
DE602004018272T DE602004018272D1 (de) 2003-02-13 2004-02-13 Fluoreszenzkorrelierte spektrometrische analyseeinrichtung
US10/545,393 US7400396B2 (en) 2003-02-13 2004-02-13 Fluorescent correalated spectrometric analysis device
US12/000,732 US7737389B2 (en) 2003-02-13 2007-12-17 Image pickup device including photodetection surface and transfer control arrangement

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2003035415 2003-02-13
JP2003-035415 2003-02-13
JP2003352638 2003-10-10
JP2003-352638 2003-10-10

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/545,393 A-371-Of-International US7400396B2 (en) 2003-02-13 2004-02-13 Fluorescent correalated spectrometric analysis device
US12/000,732 Continuation US7737389B2 (en) 2003-02-13 2007-12-17 Image pickup device including photodetection surface and transfer control arrangement

Publications (1)

Publication Number Publication Date
WO2004072624A1 true WO2004072624A1 (ja) 2004-08-26

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PCT/JP2004/001558 WO2004072624A1 (ja) 2003-02-13 2004-02-13 蛍光相関分光解析装置

Country Status (5)

Country Link
US (2) US7400396B2 (ja)
EP (2) EP1593957B1 (ja)
JP (1) JP4495083B2 (ja)
DE (1) DE602004018272D1 (ja)
WO (1) WO2004072624A1 (ja)

Cited By (8)

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WO2008087869A1 (ja) * 2007-01-16 2008-07-24 Olympus Corporation 蛍光信号解析装置および蛍光信号解析方法
US20090290780A1 (en) * 2006-07-17 2009-11-26 Karsten Kottig Analysis method for chemical and/or biological samples
JP2010517056A (ja) * 2007-01-30 2010-05-20 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 時間分解蛍光イメージングシステム
JP2010197248A (ja) * 2009-02-25 2010-09-09 Furukawa Electric Co Ltd:The ラテラルフロー型蛍光イムノクロマト法用の標的物質を定量的に検出する装置
JP2010281735A (ja) * 2009-06-05 2010-12-16 Olympus Corp 画像解析方法および画像解析装置
JP2011085485A (ja) * 2009-10-15 2011-04-28 Olympus Corp 画像解析方法および画像解析装置
JP2012529119A (ja) * 2009-06-02 2012-11-15 ソーファスト ゲーエムベーハー 超解像光ゆらぎイメージング（ｓｏｆｉ）
CN105794195A (zh) * 2014-10-27 2016-07-20 松下知识产权经营株式会社 图像形成***、图像形成方法、拍摄元件以及程序

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EP1593957B1 (en) 2003-02-13 2008-12-10 Hamamatsu Photonics K. K. Fluorescent correlated spectrometric analysis device
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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090290780A1 (en) * 2006-07-17 2009-11-26 Karsten Kottig Analysis method for chemical and/or biological samples
WO2008087869A1 (ja) * 2007-01-16 2008-07-24 Olympus Corporation 蛍光信号解析装置および蛍光信号解析方法
US8194981B2 (en) 2007-01-16 2012-06-05 Olympus Corporation Fluorescent signal analyzing apparatus and fluorescent signal analyzing method
JP5489469B2 (ja) * 2007-01-16 2014-05-14 オリンパス株式会社 蛍光信号解析装置および蛍光信号解析方法
JP2010517056A (ja) * 2007-01-30 2010-05-20 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 時間分解蛍光イメージングシステム
JP2010197248A (ja) * 2009-02-25 2010-09-09 Furukawa Electric Co Ltd:The ラテラルフロー型蛍光イムノクロマト法用の標的物質を定量的に検出する装置
JP4676539B2 (ja) * 2009-02-25 2011-04-27 古河電気工業株式会社 ラテラルフロー型蛍光イムノクロマト法用の標的物質を定量的に検出する装置及びその方法
JP2014207018A (ja) * 2009-06-02 2014-10-30 ソーファスト ゲーエムベーハー 超解像光ゆらぎイメージング（ｓｏｆｉ）
JP2012529119A (ja) * 2009-06-02 2012-11-15 ソーファスト ゲーエムベーハー 超解像光ゆらぎイメージング（ｓｏｆｉ）
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JP2010281735A (ja) * 2009-06-05 2010-12-16 Olympus Corp 画像解析方法および画像解析装置
JP2011085485A (ja) * 2009-10-15 2011-04-28 Olympus Corp 画像解析方法および画像解析装置
US8705865B2 (en) 2009-10-15 2014-04-22 Olympus Corporation Image analysis method and image analysis apparatus
CN105794195A (zh) * 2014-10-27 2016-07-20 松下知识产权经营株式会社 图像形成***、图像形成方法、拍摄元件以及程序
CN105794195B (zh) * 2014-10-27 2020-02-07 松下知识产权经营株式会社 图像形成***、图像形成方法以及拍摄元件

Also Published As

Publication number Publication date
US7400396B2 (en) 2008-07-15
JP4495083B2 (ja) 2010-06-30
DE602004018272D1 (de) 2009-01-22
EP1593957B1 (en) 2008-12-10
US20080174776A1 (en) 2008-07-24
EP1593957A1 (en) 2005-11-09
US7737389B2 (en) 2010-06-15
US20060262301A1 (en) 2006-11-23
EP1593957A4 (en) 2006-04-26
EP1925930A1 (en) 2008-05-28
JPWO2004072624A1 (ja) 2006-06-01

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