USE OF A FATTY ACID COMPOSITION FOR TREATMENT OF MALE
INFERTILITY
Technical field of the invention
The invention comprises a number of aspects. According to a first aspect of the present invention, a use of a pharmaceutical composition for prevention or treatment of male infertility, is disclosed. According to a second aspect of the invention, a use of a pharmaceutical composition for prevention or treatment of asthenozoospermia, is disclosed. According to a third aspect of the present invention, a use of a pharmaceutical composition for prevention or treatment of teratozoospermia, is disclosed. According to a fourth aspect of the invention, a use of a pharmaceutical composition for prevention or treatment of a condition due to decreased concentration of DHA in sperm, is disclosed. Moreover, according to a fifth aspect of the invention, a use of a dietary foodstuff for non-medical prevention or treatment of male infertility, is disclosed. According to a sixth aspect of the present invention, a use of a dietary foodstuff for non-medical prevention or treatment of asthenozoospermia, is disclosed. According to a seventh aspect of the invention, a use of a dietary foodstuff for non-medical prevention or treatment of teratozoospermia, is disclosed. According to eighth aspect of the present invention, a use of a dietary foodstuff for non-medical prevention or treatment of a condition due to decreased concentration of DHA in sperm, is disclosed.
Further, according to a ninth aspect of the invention, a method for prevention or treatment of male infertility, is disclosed. According to a tenth aspect of the present invention, a method for prevention or
treatment of asthenozoospermia, is disclosed. According to the eleventh aspect of the invention, a method for prevention or treatment of teratozoospermia, is disclosed. Finally, according to the twelfth aspect of the present invention, a method for prevention or treatment of a condition due to decreased concentration of DHA in sperm, is disclosed.
The aspects above are based on at least one of the following features: a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA: DHA in the fatty acid composition is 1:X, where X is > 1, or a fatty acid composition comprising at least one of EPA or DHA, to be administered to a human or an animal together with DHA in the case where the composition comprises EPA, or EPA in the case where the composition comprises DHA, wherein the total administered amount of DHA is > EPA.
Background of the invention Male infertility is responsible for approximately
45% of childless couples' inability to achieve pregnancy and no certain method of improving male fertility exists. In recent years the prognosis for infertile couples has improved but mainly by manipulation of the female such as better ways of optimising the time for conception or artificial fertilisation in uteri or in vitro. Poor sperm-forward motility (asthenozoospermia) and low number of sperms are considered to be the most common findings in infertile men with pathologic morphology (teratozoospermia) being the third most common feature. A meta-analysis of published scientific studies during the last 50 years including 14 947 men showed a significant decrease in sperm concentration during this period [Becker S and Berhane K. A meta-analysis of 61 sperm count studies revisi ted. Fertile Sterile 1997 ; 67 : 1103 - 1108] . Other studies have confirmed this finding indicating that sperm concentration has decreased by 0.8%
yearly in the US and 2.35% in Europe during the same interval [Carlsen E, et al . Evidence for decreasing quali ty of semen during the past 50 years . BMJ 1992; 305 : 609-613] . Histology of testis tissue obtained by autopsy supports the fact that the number of men with normal spermiogenesis is declining [Pajarinen J, et al . Incidence of disorders of spermatogenesis in middle aged Finnish men, 1981 -91 : two necropsy series . BMJ 1997 ; 314 : 13 -18] . The reason for this alarming finding is not known but older age by the time of family building, a dramatic change in dietary habits especially in the intake of fat, and exposition to environmental pollution have been discussed. There is certainly a need for a treatment alternative for the infertile man.
Description of prior art
Production of sperms takes place in the Leydig cells of the testicles. Their development from diploid stem cells into mobile, haploid sperm is governed by several hundred genes in which the cell nucleus becomes the sperm head while portions of the Golgi , mitochondrial , and cytoplasmic material become the tail and the sheet . The tail and head each have distinctive functions where the tail is responsible for movement in the epididymis and, after ejaculation, in the female genital tracts. The head with its acrosome plays an important role in capacitation and the acrosome reaction that renders the sperm capable of fusing with the egg plasma membrane, penetrating the egg for fertilisation and transferring the genetic material.
The protein components of sperm heads and tails have been characterised in detail [Holger H, et al . The protein complexi ty of the cytoskeleton of bovine and human sperm heads . Exp Cell Res 1995 ; 218 : 174 -182] but certain lipids also seem to play an important role in the constitution and function of sperms. Great differences in the composition of sterols, fatty acids, and the
phospholipid molecular species between heads and tails have been observed in monkeys [Connor WE, et al . Uneven distribution of desmosterol and docosahexaenoic acid in the heads and tails of monkey sperm . J Lipid Res 1998;39 : 1404 -1411] . As these lipids have an important role in membrane integrity, fluidity, stability, and permeability, these differences in membrane lipid composition may contribute to the unique functions of the sperms. The most striking difference between heads and tails were in the concentration of the omega-3 fatty acid docosahexaenoic acid (DHA, 22:6n-3)). Over 99% of the DHA was localised in the tails. The reason for this selective accumulation of DHA is not known but since high concentrations of DHA in cell membranes are correlated with membrane fluidity it is possible that this selective concentration of a polyunsaturated fatty acid (PUFA) in the tail is necessary to provide for the bending and flexing for motility of sperms. In fact it has been found that the concentration of DHA in monkey testis increased greatly during puberty suggesting the importance of this fatty acid for the maturisation of sperms [Connor WE, et al . Biochemical marker for puberty in monkey testis . J Clin Endochrin Metab 1997 ; 82 : 1911 -1916] . In fertile humans PUFA's counted for about 34% of total fatty acids and DHA contributed to more than 60% of total PUFA's [Zalata AA, et al . The fatty acid composi tion of phospholipids of spermatozoa from infertile patients . Mol Hum Reprod 1998 ;4 : 111 -118] .
DHA is the most unsaturated fatty acid of the omega- 3 series with 6 double bonds (Fig. 1) . The omega-3 fatty acids are essential to animals and humans meaning that they have to be provided in the diet . In humans the omega-3 fatty acid with the "shortest chain length alfa- linolenic acid (ALA, 18:3n-3) may be converted to DHA through the intermediate eicosapentaenoic acid (EPA,
20:5n-3, Fig. 1) by means of elongation and desaturation proposed by Sprecher (Fig. 2) The enzyme delta-6-
Figure 1
eicosapentaenoic acid (EPA)
docosahexaenoic acid (DHA)
desaturase is the rate-limiting enzyme and the conversion rate from ALA is slow in humans .
It has been reported that the capacity of the delta- 6-desaturase enzyme is reduced by age and high intake of fat in the diet. Interestingly the conversion rate of EPA to DHA is much higher indicating another mechanism of desaturation than the delta- 6 route.
Figure 2 Sprecher's pathway
As mentioned previously high age and dietary factors have been claimed as possible factors for reduced male fertility and if DHA is important for the genesis and function of sperms, delta-6-desaturase insufficiency could be the factor responsible for male infertility, at
least in people not eating fatty fish containing DHA. Seldom intake of fish is a common dietary habit throughout the Western World today.
Several studies of infertile men have demonstrated low concentrations of DHA compared to normal controls and sperm motility have showed a negative correlation to the DHA concentration [Hula NM, et al . Lipid composi tion and fertili ty of human ejaculate . Ukr Biokhim Zh 1993 ; 65 : 64 - 70, Gulaya NM, et al . Phospholipid composi tion of human sperm and seminal plasma in relation to sperm fertili ty. Arch Androl 2001 ; 46 : 169-175, Conquer JA, et al . Fatty acid analysis of blood serum, seminal plasma, and spermatozoa of normozoospermic vs . asthenozoospermic males . Lipids 1999;34 : 793 - 799] . It is interesting to notice that patients with retinitis pigmentosa where low concentrations of DHA cause visual impairment also have low concentrations of sperm DHA often coinciding with infertility [Connor WE, et al . Sperm abnormali ties in retini tis pigmentosa . Invest Ophtalmol 1997 ; 38. - 2619-2628 , Hoffman DR, et al . Impaired synthesis of docosahexaenoic acid in patients wi th x-linked retini tis pigmentosa] .
Due to the extreme unsaturation of DHA the molecule is readily oxidised. Oxidative damage of DHA has been suggested as another mechanism for low DHA concentration with possibly reduction of sperm viability [Ottero M, et al . Variation of docosahexaenoic acid content in subsets of human spermatozoa at different stages of maturation : implication for sperm lipoperoxidative damage . Mol Reprod & Devel 2000;55 : 326-334 , Zalata AA, et al . Whi te blood cells cause oxidative damage to the fatty acid composi tion of phospholipids of human spermatozoa] . Increased oxidative stress is a regular finding in elderly and individuals having a too low intake of anti- oxidative food like fruit and vegetables. In a previous study [Penny PC, et al . Boar fertili ty and reproductive performance : enhancement by fatty acid manipulation of spermatozoa . ISSFAL 2000 (abstract) P-7-
51] male breeding pigs were fed a selection of dietary supplements base on combination of different fatty acids with a high concentration of DHA together with anti- oxidants. The DHA concentration of the sperms increased significantly together with sperm number and motility. Based on these data it was believed that DHA supplementation could be used to increase the content of DHA in testicles of infertile men to improve sperm quality. To test this hypothesis a placebo-controlled study was initiated randomly allocating 28 healthy asthenozoospermic individuals to treatment with 400mg/d, 800mg/d or placebo [Conquer JA, et al . Effect of DHA supplementation on DHA status and sperm motili ty in asthenozoospermic males . Lipids 2000 ; 35 : 149-154] . Each group was treated for a period of 3 months. Semen and blood samples were collected prior to and after the 3- month treatment period.
The fatty acid profile in plasma and seminal plasma of all three groups were similar at study entry. Supplementation with DHA in both groups resulted in an increased content of DHA in plasma. However, DHA in seminal plasma did not increase or increased only slightly, the difference compared to pre-treatment levels not reaching statistical significance. The DHA content of the sperms did not change in the three groups.
Furthermore, DHA supplementation had no effects on sperm motility or sperm concentration.
Moreover, in WO 9966877 a method and a dietary supplement for increasing fertilitiy in an aniaml is presented. The method comprising feeding the animal a omega-3 fatty acid containing component, which omega-3 fatty acid containing component is preferably of plant, animal or single cell oil origin.
Summary of the invention
Based on the present invention a number of aspects are presented in the appended claims. These aspects are;
1. Use of a pharmaceutical composition for prevention or treatment of male infertility.
2. Use of a pharmaceutical composition for prevention or treatment of asthenozoospermia. 3. Use of a pharmaceutical composition for prevention or treatment of teratozoospermia. 4. Use of a pharmaceutical composition for prevention or treatment of a condition due to decreased concentration of DHA in sperm. 5. Use of a dietary foodstuff for non-medical prevention or treatment of male infertility.
6. Use of a dietary foodstuff for non-medical prevention or treatment of asthenozoospermia.
7. Use of a dietary foodstuff for non-medical prevention or treatment of teratozoospermia.
8. Use of a dietary foodstuff for non-medical prevention or treatment of a condition due to decreased concentration of DHA in sperm.
9. Method for prevention or treatment of male infertility.
10. Method for prevention or treatment of asthenozoospermia .
11. Method for prevention or treatment of teratozoospermia . 12. Method for prevention or treatment of a condition due to decreased concentration of DHA in sperm.
The aspects above are based on at least one of the following features; • a fatty acid composition comprising at least (all-Z omega-3) -5, 8, 11, 14, 17-eicosapentaenoic acid (EPA) and (all-Z omega-3) -4 , 7 , 10, 13 , 16, 19- docosahexaenoic acid (DHA) , wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1.
• a fatty acid composition comprising at least one of EPA or DHA, to be administered to a human or
an animal together with DHA in the case where the composition comprises EPA, or EPA in the case where the composition comprises DHA, wherein the total administered amount of DHA is > EPA.
According to a first aspect of the invention, the invention relates to the use of a fatty acid composition comprising at least (all-Z omega-3) -5, 8, 11, 14, 17- eicosapentaenoic acid (hereafter abbreviated EPA) and (all-Z omega-3) -4, 7, 10, 13 , 16, 19-docosahexaenoic acid
(hereafter abbreviated DHA) , wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, for the manufacture of a pharmaceutical composition for prevention or treatment of male infertility. According to a second aspect of the present invention, the invention relates to the use of a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, for the manufacture of a pharmaceutical composition for prevention or treatment of asthenozoospermia .
According to third aspect of the invention, the invention relates to the use of a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, for the manufacture of a pharmaceutical composition for prevention or treatment of teratozoospermia .
According to the fourth aspect of the invention, the invention relates to the use of a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is ≥ 1, for the manufacture of a pharmaceutical composition for prevention or treatment of a 'Condition due to decreased concentration of DHA in sperm.
In another embodiment, the prevention or treatment of male infertility includes especially prevention or
treatment of male infertility due to at least one of asthenozoospermia, teratozoospermia, and a decreased concentration of DHA in sperm or any combinations of these conditions. Moreover, the effect of the invention is accomplished by the combination of at least the two fatty acids EPA and DHA, wherein the amount of DHA is > EPA. As described in the prior art, DHA itself had no effect on sperm motility or sperm concentration, but the administration of a fatty acid composition according to the invention comprising at least EPA and DHA, wherein the weight ratio of EPA: DHA is 1:X, where X is > 1, showed a surprisingly synergetic effect. Moreover, from the research leading to the invention a most preferred effect of the invention is accomplished by a fatty acid composition rich in DHA, but still containing at least EPA. The term rich herein includes a fatty acid composition there the amount (weight or volume) of DHA is equal or larger than EPA. An advantage of the invention is that intake of a pharmaceutical composition according to the invention increases the possibilities for many men to improve their sperm motility, increase the amount of sperms respectively increase the concentration of DHA in their sperm, which will help more childless couples to achieve pregnancy.
In an other embodiment of the invention a pharmaceutical composition comprising at least EPA is to be administered together with DHA, and then the pharmaceutical composition comprising at least DHA, EPA is to be administered together with DHA, wherein the total administered amount of DHA is > EPA, in both cases for prevention or treatment of male infertility, especially male infertility due to at least one of asthenozoospermia, teratozoospermia, a decreased concentration of DHA in sperm or any combinations of
these conditions. Further, preferably the EPA and the DHA may be administered subsequently to each other.
In a preferred embodiment of the invention, the fatty acid composition is administered to a human. The pharmaceutical composition according to the invention is preferably intended to be administered to humans, but the invention also relates to use of a pharmaceutical composition according to the invention that may be administered to animals, preferably animals such as domestic animals, livestock and/or cattle.
Moreover, in another embodiment of the invention the fatty acid composition is obtained from at least one of vegetable, microbial and animal or combinations thereof.
In a preferred embodiment of the invention the EPA is obtained from at least one of vegetable, microbial and animal origins or combinations thereof, and the DHA is obtained from at least one of vegetable, microbial and animal origins or combinations thereof. Namely, it is possible that DHA and EPA may independently of each other come from different origins.
In a preferred embodiment of the invention, at least part of the EPA and/or the DHA is produced from a marine oil, preferably a fish oil.
In a more preferred embodiment of the invention both DHA and EPA is obtained from fish oil.
In another embodiment of the invention, the fatty acid composition is presented in at least one of esterified form, ethyl ester form, salt form and free acid form, or any combinations thereof. In preferred embodiments of the invention, the fatty acid composition comprises at least DHA and EPA in the forms of triglycerides, re-esterified triglycerides with content of *di- and mono-glycerides, ethyl esters, salts and/or free fatty acids or any combinations of the above mentioned forms. The rest of the fatty acid composition is preferably comprised of omega-3 -fatty acids other than DHA and EPA that have 20-22 carbon atoms in any of the
above mentioned forms. For instance, the fatty acid composition may additionally also comprise at least one of arachidonic acid (ARA) , docosapentaenoic acid, heneicosapentaenoic acid and octadecatetraenoic acid. In another embodiment, the combination of EPA:DHA are present in the composition in an EPA:DHA ratio from about 1 : 1 to 1 : 6. In a specific preferred embodiment of the invention, the weight ratio of EPA: DHA in the fatty acid composition is about 1:6. In another embodiment of the invention, 15% to 80% by weight of the fatty acid composition is comprised of EPA and DHA. Preferably, 60% to 80% by weight of the fatty acid composition is constituted by EPA and DHA. In a specific preferred embodiment about 60% by weight of the fatty acid composition is constituted by a combination of EPA and DHA.
In a preferred embodiment of the invention, the fatty acid composition also comprises (all-Z omega-3) - 7, 10, 13 , 16, 19-docosapentaenoic acid (DPA) , in an amount of about 10% to 40% of the amount of DHA in the composition. Moreover, in another embodiment of the present invention, the fatty acid composition being administered in amount providing a daily dosage of 0,5g to 6,0g of said fatty acid composition, preferably between 2g and 4g, to a human or an animal.
In a preferred embodiment of the invention the pharmaceutical composition or pharmaceutical preparation or medicinal product may preferably be produced for oral administration in the form of for instance a pill, a soft capsule or the like. However, the pharmaceutical composition or pharmaceutical preparation or medicinal product may also be produced for administration through any other route where the active ingredients may be efficiently absorbed and utilized, e.g. intravenously, subcutaneously, intramuscularely, intranasally, rectally, vaginally or topically.
The pharmaceutical composition according to the invention may also comprise other substances, in addition to the EPA and DHA active ingredients, such as vehicle, one or more acceptable carrier, fillers, stabilizers, extenders, binders, humidifiers, surfactants, lubricants etc., which all are well known to persons skilled in the art. In addition, one or more pharmaceutical acceptable antioxidants, for example hydroxytoluene, butyrate, quinone, tocopherol , ascorbic acid etc., preservatives, colouring agents, perfumes, flavourings and other pharmaceutically agents may be used. The same substances as mentioned above is also valid for a dietary food stuff according to the invention.
According to a fifth aspect of the present invention, the invention relates to the use of a fatty acid composition comprising at least (all-Z omega-3) - 5, 8, 11, 14, 17-eicosapentaenoic acid (EPA) and (all-Z omega-3) -4, 7, 10 , 13 , 16, 19-docosahexaenoic acid (DHA), wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, for the manufacture of a dietary foodstuff for non-medical prevention or treatment of male infertility.
According to a sixth aspect of the invention, the invention relates to the use of a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, for the manufacture of a dietary foodstuff for non-medical prevention or treatment of asthenozoospermia. According to a seventh aspect of the invention, the invention relates to the use of a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, for the manufacture of a dietary foodstuff for prevention or treatment of teratozoospermia. According to a eight aspect, the present invention relates to the use of a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA
in the fatty acid composition is 1:X, where X is > 1, for the manufacture of a dietary foodstuff for non-medical prevention or treatment of a condition due to decreased concentration of DHA in sperm In a preferred embodiment, the invention relates to a dietary foodstuff comprising a mixture containing at least EPA and DHA, wherein the amount of DHA > EPA, for non-medical prevention or treatment of male infertility, especially male infertility due to asthenozoospermia, teratozoospermia, low DHA concentration in sperm or combinations of these conditions.
The effect of the invention is also here accomplished by the use and administration of a fatty acid composition comprising at least a combination of EPA and DHA, wherein the weight ratio of EPA: DHA in the fatty acid composition is 1:X, where X is > 1. As described in prior art, dietary supplementation of DHA it self cannot be used to prevent or treat the conditions mentioned above, but administration of EPA and DHA together or in combination, wherein the amount of DHA is equal or larger than EPA, has a surprisingly synergetic effect.
An advantage of manufacturing and selling a dietary food stuff according to the invention for prevention and/or treatment of male infertility, especially male infertility due to asthenozoospermia, teratozoospermia, low DHA concentration in sperm or any combinations of these conditions, is that such a dietary product will be more easily accessible for men around the world. They preferably can buy the product or supplement in a health food store and/or a supermarket, and don't need to visit a doctor in the first place.
In another embodiment of the invention, the dietary foodstuff according to the invention is produced in such way that the EPA part of the composition is separate from the DHA part of the composition. In this case, an EPA- containing composition is to be administered together with DHA and a DHA-containing composition is to be
administered together with EPA, but still the total administered amount of DHA is equal or larger than the amount of EPA. Preferably, the EPA and the DHA may be administered subsequently to each other. As mentioned before, the weight ratio of EPA:DHA is also here 1:X, where X is equal or larger than 1. Please note that X being one of an integer or non-integer. Further, the dietary foodstuff according to the invention is preferably intended to be administered to humans, but it may also be administered to animals, preferably animals such as domestic animals, livestock and cattle.
In another embodiment of the invention the fatty acid composition is obtained from at least one of vegetable, microbial and animal or combinations thereof. In a preferred embodiment, at least one of EPA and
DHA is obtained from at least one of vegetable, microbial and animal origins or any combinations thereof. Further, DHA and EPA may independently of each other come from different origins. In a more preferred embodiment at least part of the EPA and/or the DHA is produced from a marine oil, preferably a fish oil. In a most preferred embodiment of the invention both DHA and EPA is obtained from fish oil .
In preferred embodiments of the invention, the dietary foodstuff comprising a mixture containing at least a fatty acid composition comprising EPA and DHA, wherein the amount of DHA is ≥ EPA, and DHA and EPA is at least in one of the form of triglycerides, re-esterified triglycerides with content of di- and mono-glycerides, ethyl esters, salts, free acids or in any combinations thereof. The rest of the fatty acid composition is preferably comprised of omega-3-fatty acids other than DHA and EPA that have 20-22 carbon atoms in any of the above mentioned forms, preferably arachidonic acid (ARA) in any of the above mentioned forms.
In another embodiment of the invention, the fatty acid composition may additionally also comprise at least
one of arachidonic acid (ARA) , docosapentaenoic acid, heneicosapentaenoic acid and octadecatetraenoic acid.
In another embodiment of the present invention, 15% to 80% by weight of the fatty acid composition is comprised of EPA and DHA. Preferably, 60% to 80% by weight of the fatty acid composition is constituted by EPA and DHA. In a specific preferred embodiment of the invention, the weight ratio of EPA:DHA in the fatty acid composition is about 1:6. In a specific preferred embodiment about 60% by weight of the fatty acid composition is constituted by a combination of EPA and DHA. For instance, a cod-liver oil or a tuna fish-liver oil may be used.
In a preferred embodiment of the invention, the fatty acid composition also comprises (all-Z omega-3) - 7, 10, 13 , 16, 19-docosapentaenoic acid (DPA) , in an amount of about 10% to 40% of the amount of DHA in the composition.
In another embodiment of the invention, the fatty acid composition being administered in amount providing a daily dosage of 0,5g to 6,0g of said fatty acid composition, preferably between 2g and 4g.
In a preferred embodiment, the dietary food stuff is in form of a gelatine capsule, which capsule is flavoured. Moreover, preferably the active compounds according to the dietary foodstuff of the invention may be administered in one of the possible forms and ways as mentioned before for the pharmaceutical composition according to the invention. In addition, the dietary foodstuff according to the invention may comprise, in addition to the EPA and DHA ingredients, at least one of the same substances as mentioned before according to a pharmaceutical composition, but also other suitable agents such as vitamins and/or minerals. According to a ninth aspect of the invention, the invention relates to a method for prevention or treatment of male infertility, wherein a therapeutically active
amount of a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, is administrated to a patient in a need of said treatment. According to a tenth aspect of the invention, the invention relates to a method for prevention or treatment of asthenozoospermia, wherein a therapeutically active amount of a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, is administrated to a patient in a need of said treatment.
According to a eleventh aspect of the invention, the invention relates to a method for prevention or treatment of teratozoospermia, wherein a therapeutically active amount of a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, is administrated to a patient in a need of said treatment . According to a twelth aspect of the invention, the invention relates to a method for prevention or treatment of a condition due to decreased concentration of DHA in sperm, wherein a therapeutically active amount of a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, is administrated to a patient in a need of said treatment. In another embodiment, the invention relates to a method for prevention and/or treatment of male infertility, especially male infertility due to asthenozoospermia, teratozoospermia, decreased concentration of DHA in sperm or combinations of these conditions, wherein a therapeutically or pharmaceutically active amount of at least a fatty acid composition comprising EPA and DHA, wherein the amount of DHA is > EPA, is administrated to a patient in a need of the mentioned treatment. The effect of the invention is accomplished by preferably the combination of the two
fatty acids EPA and DHA, but the amount of DHA may be equal or larger than EPA.
In another embodiment of the invention a therapeutically or pharmaceutically active amount of a fatty acid composition comprising at least EPA is to be administered together with DHA, and then the composition comprising at least DHA, EPA is to be administered together with DHA, wherein the total administered amount of DHA is > EPA, in both cases for prevention or treatment of male infertility, especially male infertility due to at least one of asthenozoospermia, teratozoospermia, a decreased concentration of DHA in sperm or any combinations of these conditions.
In another embodiment of the invention, at least one of EPA and DHA is obtained from at least one of vegetable, microbial and animal origins or combinations thereof. In a preferred embodiment of the invention, at least part of the EPA and/or the DHA is produced from a marine oil, preferably a fish oil. In a preferred embodiments of the invention, the therapeutically or pharmaceutically active amount of a fatty acid composition comprising at least EPA and DHA is administrated to a patient, wherein the DHA and EPA is in one of the forms of triglycerides, re-esterified triglycerides with content of di- and mono-glycerides, ethyl esters, salts, free acids or any combinations thereof. The rest of the fatty acid composition is preferably comprised of omega-3-fatty acids other than DHA and EPA that have 20-22 carbon atoms in any of the above mentioned forms.
In another embodiment of the invention, 15% to 80% by weight of the fatty acid composition is comprised of EPA and DHA. In a preferred embodiment of the invention, 60% to 80% by weight of the fatty acid composition is comprised of EPA and DHA.
In another embodiment of the invention, the fatty acid composition also comprises (all-Z omega-3) -
7, 10, 13 , 16, 19-docosapentaenoic acid (DPA) , in an amount of about 10% to 40% of the amount of DHA in the composition. Moreover, in another embodiment of the invention, the pharmaceutically or therapeutically active composition is administered in amount providing a daily dosage of 0,5 to 6,0 grams of the fatty acid composition according to the invention.
In a preferred embodiment of the invention the fatty acid composition is administered in a daily dosage of 2 to 4g and most preferably about 3g.
In a preferred embodiment of the invention, the pharmaceutically active composition is administered in a daily dosage comprising about 450-850mg EPA and about l,7-3,0g DHA. In a specific preferred embodiment of the invention the dosage of EPA is about 0,8g and the dosage of DHA is about l,9g.
In a preferred embodiment, the pharmaceutically active compound according to the invention is administered in a food product or as a dietary foodstuff. The analysis in % by weight is based on the ethyl esters even if other derivatives of salts or the acids themselves are a part of the present invention.
Definitions As used herein the term manufacture is interpreted to include production of a pharmaceutical composition and/or a medicinal product. Moreover, as used herein the term asthenozoospermia means poor sperm forward motility. Further, as used herein the term pharmaceutical means pharmaceutical preparations and/or compositions and medical food. A pharmaceutical preparation according to the present invention may also comprise other substances such as an inert vehicle or a pharmaceutically acceptable adjuvance, carriers, preservatives etc., which all are well-known to those skilled in the art.
As used herein, the term dietary foodstuff is interpreted to include food and food supplement to
animals and/or humans, fortification of food, dietary supplement, functional food and nutrient supplement.
As used herein the term patient relates to any human or non-human mammal in need of treatment with the medicinal product or method according to the invention. As used herein, the term marine oil includes oil from fish, seals, cetaceans, sea shell and sea mammals. Non limiting examples of fish oil is e.g. Menhaden oil, Cod Liver oil, Herring oil, Capelin oil, Sardine oil, Anchovy oil and Salmon oil. The fish oils mentioned above may be recovered from fish organs, e.g. cod liver oil, as well as from the meat of the. fish.
As used herein, the term microbial oils also includes "single cell oils" and blends, or mixtures, containing unmodified microbial oils. Microbial oils and single cell oils are those oils naturally produced by micro organisms during their lifespan.
As used herein, the term treatment means both treatment having a curving or alleviating purpose and treatment having a preventive purpose. The treatment can be made either acutely or chronically. Treatment can be given to both humans and animals. Non-limiting examples of such animals is e.g. domestic animals, livestock and cattle . As used herein, the term prevention means actions having a preventive purpose to prevent a condition to appear. Preventive actions can be given to both humans and animals.
Brief description of the drawings
In the studies and examples below reference is made to the accompanying drawings, where all figures concern studies performed on human. The studies were preformed in order to demonstrate improved sperm motility and morphology in fertile men. Herein, reference is made to accompanying drawings, on with:
Figure 3 shows the levels (%) of DHA in plasma total phospholipid.
Figure 4 shows the levels (%) of DHA in sperm total phospholipid . Figure 5 shows the effect on sperm motility.
Figure 6 shows the effect on abnormal morphology. Figure 7 shows the relationship between total sperm count (mill) and DHA (%) in sperm total phospholipid. Finally, figure 8 shows the relationship between DHA (%) in sperm total phospholipid and DHA (%) in plasma total phospholipides .
Example
The invention will now be further explained in the following examples. The examples are only intended to illustrate the invention and should in no way be considered to limit the scope of the invention.
Study 1 In order to evaluate the effect of a fatty acid composition according to the present invention on male infertility, 16 asthenozoospermia men, aged 30-40 years and living in the Reykjavik, Island, area, with sperm motility lower than 30% of normal value volunteered to participate in an open intervention using a 60% of weight omega-3 concentrate from fish. The concentrate (EPAX 2050TG) contained 20% eicosapentaenoic acid (EPA) and 50% docosahexaenoic acid (DHA) as re-esterified triglyceride with a triglyceride content of 50-55%, diglyceride content of 40-45% and monoglyceride content of about 5%. Namely, a fatty acid composition comprising at least EPA and DHA, wherein the amount of DHA is larger than EPA, was used. The participants had not consumed any other omega-3 preparation or cod liver oil at least two years prior to the trial. They did not use medication and none were heavy smokers or drinkers .
Blood and semen samples were collected two weeks before study start (A) , the day the trial started (B) , at 10 weeks (C) and at 20 weeks (D) after study start. After completion of the study, the participants were controlled after two (E) and 10 weeks (F) . All together the participants were controlled six times. The frequent controls were warranted to compensate for anticipated large variations in study parameters . During the treatment period, the participants were given six capsules of the omega-3 concentrate daily, each capsule containing 750 mg oil, during 20 weeks. The daily dose of EPA was 0,81 g and DHA 1,89 g. The following parameters were measured for each sample: Sperm count, sperm motility, sperm morphology, semen viscosity, fatty acid composition of sperm phospholipids, fatty acids in blood plasma and lipid peroxides.
The study shows that the supplementation with a fatty acid composition comprising at least EPA and DHA, wherein the amount of DHA is larger than EPA, increases the average concentration of DHA in the plasma phospholipids, as evident from figure 3. Moreover, the distribution of the amount of DHA in sperm phospholipides in patients at study start, after 10 weeks and 20 weeks of treatment respectively 10 weeks after the completion of the study, is shown in figure 4. The best effect seems to be obtained after 10 weeks of treatment.
Moreover, it should be pointed out that participants with the best sperm motility (least pathological values) at study entry demonstrated an improvement in sperm motility from pre-study values to values during treatment, as evident from figure 5. The positive values were retained also after the study. The administration of the fatty acid composition did not show an effect on those participants with the poorest motility before study start.
In addition, abnormal sperm morphology was reduced during treatment and the positive effects were retained
after completion of the study, as seen in fig 5. The best results were obtained two weeks after completion of the study. Normal morphology increased concomitantly with the best values two weeks after study completion. This result shows that treatment with a fatty acid composition according to the invention increases the possibilities for many men to improve their sperm motility, increase the amount of sperms respectively increase the concentration of DHA in their sperm, which will help more childless couples to achieve pregnancy.
Study 2
In this study, the relationship between total sperm count (mill) and DHA (%) in sperm total phospholipid and the relationship between DHA (%) in sperm total phospholipid and DHA (%) in plasma total phospholipid for three participants were studied. Herein, participants one and three were non-smokers, and participant 2 was a smoker. The study was carried out in the same way as mentioned in study 1 and the participants were given the same fatty acid composition, a fatty acid composition rich in DHA compared to EPA. Samples were collected two weeks before study start, the day the trial started, at 10 weeks and at 20 weeks after study start. After completion of the study, the participants were controlled after two and 10 weeks. All together the participants were controlled six times.
Figure 7 shows that the total sperm count (mill) is correlated to the level (%) of DHA in sperm total phospholipid. In participants one and three the treatment with a fatty acid composition according to the invention had an effect on the DHA level of sperm total phospholipid as well as the sperm count. Moreover, figure 8 illustrates the relationship between DHA (%) in sperm total phospholipid and DHA (%) in plasma total phospholipid after 20 weeks of treatment according to the invention.
Discussion
The result shows that treatment with a fatty acid composition comprising at least EPA and DHA, wherein the weight ratio of EPA:DHA in the fatty acid composition is 1:X, where X is > 1, improved sperm motility and morphology in infertile men. Moreover, the DHA concentrations increased in almost every participant. These findings are unexpected and differ significantly from the previous study in humans using DHA alone. From animal and human studies it is documented that DHA is enriched in sperms and that infertile men are deprived of sperm DHA. Based on prior art and the present study it is obvious that DHA supplementation alone cannot increase sperm DHA and normalise sperm motility and morphology. Thus, a fatty acid compositions according to the present invention are potentially valuable for the treatment and prophylaxis of male infertility or multiple factors with influence on male infertility, such as asthenozoospermia, teratozoospermia, low concentration of
DHA or the like.
The doses of the composition of this invention needed for therapeutic or prophylactic effect will vary with the type of administration, body size, and the seriousness of the condition to be treated.
It is advantageous that the compositions according to the present invention are being well tolerated, not giving any severe side effects.
Example of pharmaceutical preparation
Soft gelatine capsules containing 0.75 g/capsule in prepared. Composition:
EPA 180 mg/capsule
DHA 420 mg/capsule Covitol F-1000 3 mg/capsule
Coviox T-70 1.1 mg/capsule
Tocopherol 4 mg/capsule
The rest of the fatty acid composition is preferably essentially comprised of omega-3 -fatty acids other than DHA and EPA that have 20-22 carbon atoms in any of the above mentioned forms, preferably at least DPA.
The capsule may be micro- or macro incapsulated. Further, the capsule may be constituted by one of bovine gelatine, fish gelatine or pig gelatine. Alternatively, a vegetable capsule may be used. The active ingredients and the excpeciants are weighed an homogenized on a high speed stirrer. The mixture is then colloid milled and dereated in a stainless steel vessel ready for encapsulation. The mixture is filled in soft gelatine capsules of size 20 oblong (average weight 1.2 g) using a standard capsulation machine.
The invention shall not be limited to the above shown embodiments and examples, but shall be interpreted within the scope of the appending claims.