WO2004024929A3 - Proximity-aided synthesis of templated molecules - Google Patents

Proximity-aided synthesis of templated molecules Download PDF

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Publication number
WO2004024929A3
WO2004024929A3 PCT/DK2003/000590 DK0300590W WO2004024929A3 WO 2004024929 A3 WO2004024929 A3 WO 2004024929A3 DK 0300590 W DK0300590 W DK 0300590W WO 2004024929 A3 WO2004024929 A3 WO 2004024929A3
Authority
WO
WIPO (PCT)
Prior art keywords
template
codon
reactive group
building block
functional entity
Prior art date
Application number
PCT/DK2003/000590
Other languages
French (fr)
Other versions
WO2004024929A2 (en
Inventor
Thomas Franch
Thomas Thisted
Original Assignee
Nuevolution As
Thomas Franch
Thomas Thisted
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nuevolution As, Thomas Franch, Thomas Thisted filed Critical Nuevolution As
Priority to EP03794823A priority Critical patent/EP1539953A2/en
Priority to AU2003260291A priority patent/AU2003260291A1/en
Publication of WO2004024929A2 publication Critical patent/WO2004024929A2/en
Publication of WO2004024929A3 publication Critical patent/WO2004024929A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis

Abstract

Disclosed is a method for synthesising a bifunctional complex. The complex comprises a template as well as a molecule, the synthesis of which being directed by the template. The synthesis of he complex requires initially a) a template comprising two or more codons in sequence, a first pair of a molecular affinity pair, and a reactive group; and b) two or more building blocks, each building block comprises i) an anti-codon capable of recognising a codon of the template, ii) a functional entity comprising at least one reactive group, and iii) a linker connecting the anti-codon and the functional entity, wherein building blocks having anti-codons intended to interact with codons of the template distal to the reactive group comprise as a section of the linker a second part of the molecular affinity pair. The synthesis proceeds by c) contacting the template with a building block under conditions which allow specific hybridisation of the anti-codon of the building block to the codon of the template, and under conditions ensuring assembling of the parts of the molecule pair, if present; d) obtaining a connection between the functional entity of the building block and the template by a reaction involving the template reactive group and the functional entity reactive group, e) cleaving a linkage to obtaining a nascent templated molecule, f) separating the parts of the molecular affinity pair, and g) repeating, for a building block having an anti-codon capable of hybridising to a new codon, steps c) to f) one or more times. The complexes obtainable according the invention may be used in the generation of a library which may be enriched with regard to preferred complexes using molecular evolution techniques.
PCT/DK2003/000590 2002-09-12 2003-09-12 Proximity-aided synthesis of templated molecules WO2004024929A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP03794823A EP1539953A2 (en) 2002-09-12 2003-09-12 Proximity-aided synthesis of templated molecules
AU2003260291A AU2003260291A1 (en) 2002-09-12 2003-09-12 Proximity-aided synthesis of templated molecules

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US40996802P 2002-09-12 2002-09-12
US60/409,968 2002-09-12
DKPA200201347 2002-09-12
DKPA200201347 2002-09-12

Publications (2)

Publication Number Publication Date
WO2004024929A2 WO2004024929A2 (en) 2004-03-25
WO2004024929A3 true WO2004024929A3 (en) 2004-05-06

Family

ID=31995414

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2003/000590 WO2004024929A2 (en) 2002-09-12 2003-09-12 Proximity-aided synthesis of templated molecules

Country Status (3)

Country Link
EP (1) EP1539953A2 (en)
AU (1) AU2003260291A1 (en)
WO (1) WO2004024929A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8206901B2 (en) 2002-10-30 2012-06-26 Nuevolution A/S Method for the synthesis of a bifunctional complex
US8808984B2 (en) 2002-03-15 2014-08-19 Neuvolution A/S Method for synthesising templated molecules
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004013070A2 (en) 2002-08-01 2004-02-12 Nuevolution A/S Multi-step synthesis of templated molecules
AU2011226815B2 (en) * 2002-10-30 2014-09-25 Nuevolution A/S Enzymatic encoding
EP1597395A2 (en) 2003-02-21 2005-11-23 Nuevolution A/S Method for producing second-generation library
ATE424455T1 (en) 2003-03-20 2009-03-15 Nuevolution As LIGATION-MEDIATING CODING OF SMALL MOLECULES
EP1670939B1 (en) * 2003-09-18 2009-11-04 Nuevolution A/S A method for obtaining structural information concerning an encoded molecule and method for selecting compounds
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
WO2005058479A2 (en) 2003-12-17 2005-06-30 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
ATE447020T1 (en) 2004-03-22 2009-11-15 Nuevolution As LIGATION CODING USING OLIGONUCLEOTIDE BUILDING BLOCKS
CA2587010C (en) * 2004-11-08 2017-08-15 Vipergen Aps Structural nucleic acid guided chemical synthesis
DE602006013134D1 (en) 2005-06-17 2010-05-06 Harvard College ITERATED ADDRESSING REACTION TRANSACTIONS OF NUCLEIC ACID-MEDIATED CHEMISTRY
EP1940755A2 (en) 2005-10-28 2008-07-09 Praecis Pharmaceuticals Inc. Methods for identifying compounds of interest using encoded libraries
SG2014011381A (en) 2009-02-13 2014-06-27 Chem Inc X Methods of creating and screening dna-encoded libraries
ES2713873T3 (en) 2010-04-16 2019-05-24 Nuevolution As Bifunctional complexes and methods for making and using such complexes
EP3828271A1 (en) 2011-09-07 2021-06-02 X-Chem, Inc. Methods for tagging dna-encoded libraries
EA034141B1 (en) 2012-07-13 2020-01-09 Икс-Чем, Инк. Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
WO2020051452A2 (en) * 2018-09-07 2020-03-12 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Hiv or hcv detection with crispr-cas13a

Citations (3)

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Publication number Priority date Publication date Assignee Title
WO1993020242A1 (en) * 1992-03-30 1993-10-14 The Scripps Research Institute Encoded combinatorial chemical libraries
WO2000023458A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
WO2002074929A2 (en) * 2001-03-19 2002-09-26 President And Fellows Of Harvard College Evolving new molecular function

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
IL163822A0 (en) * 2002-03-15 2005-12-18 Nuevolution As An improved method for synthesising templated molecules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993020242A1 (en) * 1992-03-30 1993-10-14 The Scripps Research Institute Encoded combinatorial chemical libraries
WO2000023458A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
WO2002074929A2 (en) * 2001-03-19 2002-09-26 President And Fellows Of Harvard College Evolving new molecular function

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GARTNER Z J ET AL: "Multistep small-molecule synthesis programmed by DNA templates", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 124, no. 35, 4 September 2002 (2002-09-04), pages 10304 - 10306, XP002265219, ISSN: 0002-7863 *
GARTNER Z J ET AL: "THE GENERALITY OF DNA-TEMPLATED SYNTHESIS AS A BASIS FOR EVOLVING NON-NATURAL SMALL MOLECULES", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 123, 2001, pages 6961 - 6963, XP001127054, ISSN: 0002-7863 *
See also references of EP1539953A2 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US8808984B2 (en) 2002-03-15 2014-08-19 Neuvolution A/S Method for synthesising templated molecules
US8206901B2 (en) 2002-10-30 2012-06-26 Nuevolution A/S Method for the synthesis of a bifunctional complex
US8722583B2 (en) 2002-10-30 2014-05-13 Nuevolution A/S Method for selecting a chemical entity from a tagged library
US9109248B2 (en) 2002-10-30 2015-08-18 Nuevolution A/S Method for the synthesis of a bifunctional complex
US9284600B2 (en) 2002-10-30 2016-03-15 Neuvolution A/S Method for the synthesis of a bifunctional complex
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Also Published As

Publication number Publication date
EP1539953A2 (en) 2005-06-15
WO2004024929A2 (en) 2004-03-25
AU2003260291A1 (en) 2004-04-30
AU2003260291A8 (en) 2004-04-30

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