WO2004011489A3 - Tropism-modified adenoviral vectors, preferably for targeting b-lymphocytes or ovarian cells - Google Patents

Tropism-modified adenoviral vectors, preferably for targeting b-lymphocytes or ovarian cells Download PDF

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Publication number
WO2004011489A3
WO2004011489A3 PCT/FR2003/002315 FR0302315W WO2004011489A3 WO 2004011489 A3 WO2004011489 A3 WO 2004011489A3 FR 0302315 W FR0302315 W FR 0302315W WO 2004011489 A3 WO2004011489 A3 WO 2004011489A3
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Prior art keywords
lymphocytes
adenovirus
fiber
tropism
cells
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PCT/FR2003/002315
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French (fr)
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WO2004011489A2 (en
Inventor
Halluin Jean Claude D
Laurence Renaut
Morvane Colin
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Inst Nat Sante Rech Med
Halluin Jean Claude D
Laurence Renaut
Morvane Colin
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Application filed by Inst Nat Sante Rech Med, Halluin Jean Claude D, Laurence Renaut, Morvane Colin filed Critical Inst Nat Sante Rech Med
Priority to AU2003273463A priority Critical patent/AU2003273463A1/en
Publication of WO2004011489A2 publication Critical patent/WO2004011489A2/en
Publication of WO2004011489A3 publication Critical patent/WO2004011489A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10345—Special targeting system for viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16211—Lymphocryptovirus, e.g. human herpesvirus 4, Epstein-Barr Virus
    • C12N2710/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention concerns recombinant adenoviruses whereof the tropism has been modified to enable B-lymphocytes or ovarian cells to be infected thereby. The inventive recombinant adenoviruses exhibit a reduced tropism compared to that of the native adenovirus. The invention also concerns methods for preparing said adenoviruses, as well as their uses, in particular for transferring DNA sequences into B-lymphocytes or ovarian cells in vitro, ex vivo or in vivo, for experimental, industrial, vaccination or therapeutic purposes. The invention more specifically concerns the modification of the gene of the adenovirus fiber for targeting B-lymphocytes or ovarian cells, in particular tumor cells, as well as other modifications and/or constructs enabling the viruses to be provided with a character specific to said cells. The invention is particularly characterized either by insertion into the fiber of the sequence of a peptide identifying a receptor expressed at the surface of B-lymphocytes, or by replacing the Mastadenovirus adenovirus fiber bud with a fiber bud of a phylogenetically distant adenovirus, preferably of the bovine adenovirus serotype 4 (BAV4).
PCT/FR2003/002315 2002-07-25 2003-07-22 Tropism-modified adenoviral vectors, preferably for targeting b-lymphocytes or ovarian cells WO2004011489A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003273463A AU2003273463A1 (en) 2002-07-25 2003-07-22 Tropism-modified adenoviral vectors, preferably for targeting b-lymphocytes or ovarian cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR02/09426 2002-07-25
FR0209426A FR2842823A1 (en) 2002-07-25 2002-07-25 MODIFIED ADENOVIRUSES FOR TARGETING B-LYMPHOCYTES

Publications (2)

Publication Number Publication Date
WO2004011489A2 WO2004011489A2 (en) 2004-02-05
WO2004011489A3 true WO2004011489A3 (en) 2004-07-01

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PCT/FR2003/002315 WO2004011489A2 (en) 2002-07-25 2003-07-22 Tropism-modified adenoviral vectors, preferably for targeting b-lymphocytes or ovarian cells

Country Status (3)

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AU (1) AU2003273463A1 (en)
FR (1) FR2842823A1 (en)
WO (1) WO2004011489A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4876165B2 (en) * 2006-03-28 2012-02-15 ザ プロクター アンド ギャンブル カンパニー Hydride reduction method to prepare quinolone intermediates

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997006826A1 (en) * 1995-08-14 1997-02-27 Commonwealth Scientific And Industrial Research Organisation Gene therapy using ovine adenoviral vectors
WO1998040508A1 (en) * 1997-03-14 1998-09-17 Selective Genetics, Inc. Adenoviral vectors with modified tropism
WO1998054346A1 (en) * 1997-05-28 1998-12-03 Genvec, Inc. Alternatively targeted adenovirus
WO1999047180A1 (en) * 1998-03-20 1999-09-23 Genzyme Corporation Chimeric adenoviral vectors for targeted gene delivery
WO2000073478A2 (en) * 1999-06-01 2000-12-07 University Of Washington Recombinant adenoviral vectors expressing chimeric fiber proteins for cell specific infection and genome integration
WO2001092547A2 (en) * 2000-05-31 2001-12-06 University Of Saskatchewan Modified bovine adenovirus having altered tropism

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997006826A1 (en) * 1995-08-14 1997-02-27 Commonwealth Scientific And Industrial Research Organisation Gene therapy using ovine adenoviral vectors
WO1998040508A1 (en) * 1997-03-14 1998-09-17 Selective Genetics, Inc. Adenoviral vectors with modified tropism
WO1998054346A1 (en) * 1997-05-28 1998-12-03 Genvec, Inc. Alternatively targeted adenovirus
WO1999047180A1 (en) * 1998-03-20 1999-09-23 Genzyme Corporation Chimeric adenoviral vectors for targeted gene delivery
WO2000073478A2 (en) * 1999-06-01 2000-12-07 University Of Washington Recombinant adenoviral vectors expressing chimeric fiber proteins for cell specific infection and genome integration
WO2001092547A2 (en) * 2000-05-31 2001-12-06 University Of Saskatchewan Modified bovine adenovirus having altered tropism

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
CURIEL D T: "STRATEGIES TO ADAPT ADENOVIRAL VECTORS FOR TARGETED DELIVERY", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, NEW YORK ACADEMY OF SCIENCES, NEW YORK, NY, US, vol. 886, 1999, pages 158 - 171, XP000979449, ISSN: 0077-8923 *
DAN ADAM ET AL: "Analysis of the hexon gene sequence of bovine adenovirus type 4 provides further support for a new adenovirus genus (Atadenovirus)", JOURNAL OF GENERAL VIROLOGY, vol. 79, no. 6, June 1998 (1998-06-01), pages 1453 - 1460, XP002276148, ISSN: 0022-1317 *
DATABASE GENBANK [online] NCBI; 16 April 2001 (2001-04-16), DAN A ET AL.: "Bovine Adenovirus 4", XP002276150, Database accession no. AF036092 *
HESS MICHAEL ET AL: "The complete nucleotide sequence of the egg drop syndrome virus: An intermediate between mastadenoviruses and aviadenoviruses", VIROLOGY, vol. 238, no. 1, 10 November 1997 (1997-11-10), pages 145 - 156, XP004460036, ISSN: 0042-6822 *
ISRAEL BRUCE F ET AL: "Enhancement of adenovirus vector entry into CD70-positive B-cell lines by using a bispecific CD70-adenovirus fiber antibody", JOURNAL OF VIROLOGY, vol. 75, no. 11, June 2001 (2001-06-01), pages 5215 - 5221, XP002265173, ISSN: 0022-538X *
KIRBY I ET AL: "MUTATIONS IN THE DG LOOP OF ADENOVIRUS TYPE 5 FIBER KNOB PROTEIN ABOLISH HIGH-AFFINITY BINDING TO ITS CELLULAR RECEPTOR CAR", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 73, no. 11, November 1999 (1999-11-01), pages 9508 - 9514, XP000984778, ISSN: 0022-538X *
KRASNYKH VICTOR ET AL: "Genetic targeting of an adenovirus vector via replacement of the fiber protein with the phage T4 fibritin", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 75, no. 9, May 2001 (2001-05-01), pages 4176 - 4183, XP002202288, ISSN: 0022-538X *
SEGERMAN A ET AL: "Adenovirus types 11p and 35p show high binding efficiencies for committed hematopoietic cell lines and are infective to these cell lines", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 74, no. 3, February 2000 (2000-02-01), pages 1457 - 1467, XP002161682, ISSN: 0022-538X *
SEGGERN VON D J ET AL: "ADENOVIRUS VECTOR PSEUDOTYPING IN FIBER-EXPRESSING CELL LINES: IMPROVED TRANSDUCTION OF EPSTEIN-BARR VIRUS-TRANSFORMED B CELLS", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 74, no. 1, January 2000 (2000-01-01), pages 354 - 362, XP000906911, ISSN: 0022-538X *
WICKHAM T J ET AL: "Increased in vitro and in vivo gene transfer by adenovirus vectors containing chimeric fiber proteins", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 71, no. 11, 1 November 1997 (1997-11-01), pages 8221 - 8229, XP002078898, ISSN: 0022-538X *
WICKHAM T J: "TARGETING ADENOVIRUS", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, vol. 7, no. 2, January 2000 (2000-01-01), pages 110 - 114, XP001055892, ISSN: 0969-7128 *

Also Published As

Publication number Publication date
AU2003273463A1 (en) 2004-02-16
AU2003273463A8 (en) 2004-02-16
FR2842823A1 (en) 2004-01-30
WO2004011489A2 (en) 2004-02-05

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