WO2003095632A1 - Method and system for obtaining cells of specific type from biological material - Google Patents

Method and system for obtaining cells of specific type from biological material Download PDF

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Publication number
WO2003095632A1
WO2003095632A1 PCT/JP2003/005713 JP0305713W WO03095632A1 WO 2003095632 A1 WO2003095632 A1 WO 2003095632A1 JP 0305713 W JP0305713 W JP 0305713W WO 03095632 A1 WO03095632 A1 WO 03095632A1
Authority
WO
WIPO (PCT)
Prior art keywords
biological material
specific type
cells
pcm
digestion chamber
Prior art date
Application number
PCT/JP2003/005713
Other languages
French (fr)
Japanese (ja)
Inventor
Yoshihiro Hatanaka
Takaaki Saeki
Masaaki Miyamoto
Kazuo Umihira
Original Assignee
Asahi Kasei Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2002131348A external-priority patent/JP2003319774A/en
Priority claimed from JP2002131366A external-priority patent/JP2003319775A/en
Priority claimed from JP2002190161A external-priority patent/JP2004033006A/en
Priority claimed from JP2002254185A external-priority patent/JP4414640B2/en
Application filed by Asahi Kasei Kabushiki Kaisha filed Critical Asahi Kasei Kabushiki Kaisha
Priority to AU2003235875A priority Critical patent/AU2003235875A1/en
Publication of WO2003095632A1 publication Critical patent/WO2003095632A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

It is intended to disclose a method and a system for obtaining cells of a specific type from a biological material which comprise: introducing the biological material into a digestion chamber having a screen, through which cells or cell clusters of a specific type can pass but the biological material cannot pass, at the outlet; continuously feeding a first physiologically compatible medium (PCM) into the digestion chamber to digest the biological material; the digestion of the biological material being carry out while continuously withdrawing the first PCM from the digestion chamber but the thus withdrawn PCM never being fed into the digestion chamber again; the continuous feeding of the first PCM being carried out within a time sufficient for separating the cells of the specific type from the biological material, transferring the cells and thus allowing to pass through the screen to thereby start the passage of the cells of the specific type through the screen; continuously feeding a second PCM into the chamber; continuously recovering a cell suspension containing the cells of the specific type from the digestion chamber; and then separating the cells of the specific type from the cell suspension.
PCT/JP2003/005713 2002-05-07 2003-05-07 Method and system for obtaining cells of specific type from biological material WO2003095632A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003235875A AU2003235875A1 (en) 2002-05-07 2003-05-07 Method and system for obtaining cells of specific type from biological material

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
JP2002131348A JP2003319774A (en) 2002-05-07 2002-05-07 Method for concentrating cell
JP2002131366A JP2003319775A (en) 2002-05-07 2002-05-07 Method for separating and recovering cell
JP2002-131348 2002-05-07
JP2002-131366 2002-05-07
JP2002190161A JP2004033006A (en) 2002-06-28 2002-06-28 Method for digesting organism-derived material/recovering cell and apparatus therefor
JP2002-190161 2002-06-28
JP2002254185A JP4414640B2 (en) 2002-08-30 2002-08-30 Cell detection method and apparatus
JP2002-254185 2002-08-30

Publications (1)

Publication Number Publication Date
WO2003095632A1 true WO2003095632A1 (en) 2003-11-20

Family

ID=29424658

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2003/005713 WO2003095632A1 (en) 2002-05-07 2003-05-07 Method and system for obtaining cells of specific type from biological material

Country Status (2)

Country Link
AU (1) AU2003235875A1 (en)
WO (1) WO2003095632A1 (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988009667A1 (en) * 1987-06-08 1988-12-15 Washington University Method to isolate cell clusters of cells subtypes from organs
WO1994003645A1 (en) * 1992-07-31 1994-02-17 Thomas Jefferson University Device and method for processing fat tissue to produce endothelial cell product
EP0723009A1 (en) * 1995-01-17 1996-07-24 HOSPAL R&D Int. A device for organ digestion and a procedure for the isolation of biologically interesting cells or cell-clusters from an organ
JPH0956384A (en) * 1995-08-25 1997-03-04 Toray Ind Inc Labeling of cell
JPH10215894A (en) * 1997-02-06 1998-08-18 Mitsubishi Chem Corp Detection of live cell
US5837444A (en) * 1996-07-16 1998-11-17 Shah; Kumarpal A. Islet cell transplantation machine for diabetes cure
JP2000078999A (en) * 1998-09-04 2000-03-21 Monotekku Kk Method and apparatus for detecting microorganism
WO2000048703A1 (en) * 1999-02-22 2000-08-24 Henry Kopf Purification of biological substances

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988009667A1 (en) * 1987-06-08 1988-12-15 Washington University Method to isolate cell clusters of cells subtypes from organs
WO1994003645A1 (en) * 1992-07-31 1994-02-17 Thomas Jefferson University Device and method for processing fat tissue to produce endothelial cell product
EP0723009A1 (en) * 1995-01-17 1996-07-24 HOSPAL R&D Int. A device for organ digestion and a procedure for the isolation of biologically interesting cells or cell-clusters from an organ
JPH0956384A (en) * 1995-08-25 1997-03-04 Toray Ind Inc Labeling of cell
US5837444A (en) * 1996-07-16 1998-11-17 Shah; Kumarpal A. Islet cell transplantation machine for diabetes cure
JPH10215894A (en) * 1997-02-06 1998-08-18 Mitsubishi Chem Corp Detection of live cell
JP2000078999A (en) * 1998-09-04 2000-03-21 Monotekku Kk Method and apparatus for detecting microorganism
WO2000048703A1 (en) * 1999-02-22 2000-08-24 Henry Kopf Purification of biological substances

Also Published As

Publication number Publication date
AU2003235875A1 (en) 2003-11-11

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