WO2003078577A8 - Cloning of cytochrome p450 genes from nicotiana - Google Patents

Cloning of cytochrome p450 genes from nicotiana

Info

Publication number
WO2003078577A8
WO2003078577A8 PCT/US2003/007430 US0307430W WO03078577A8 WO 2003078577 A8 WO2003078577 A8 WO 2003078577A8 US 0307430 W US0307430 W US 0307430W WO 03078577 A8 WO03078577 A8 WO 03078577A8
Authority
WO
WIPO (PCT)
Prior art keywords
nicotiana
cytochrome
cloning
genes
Prior art date
Application number
PCT/US2003/007430
Other languages
French (fr)
Other versions
WO2003078577A2 (en
WO2003078577A9 (en
Inventor
Dongmei Xu
Original Assignee
Us Smokeless Tobacco Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Smokeless Tobacco Co filed Critical Us Smokeless Tobacco Co
Priority to JP2003576572A priority Critical patent/JP2005531297A/en
Priority to CA002477452A priority patent/CA2477452A1/en
Priority to KR10-2004-7014250A priority patent/KR20040091124A/en
Priority to EP03716460A priority patent/EP1572911A4/en
Priority to MXPA04008785A priority patent/MXPA04008785A/en
Priority to BRPI0308351-9A priority patent/BR0308351A/en
Priority to APAP/P/2004/003122A priority patent/AP2004003122A0/en
Priority to AU2003220165A priority patent/AU2003220165A1/en
Publication of WO2003078577A2 publication Critical patent/WO2003078577A2/en
Publication of WO2003078577A9 publication Critical patent/WO2003078577A9/en
Publication of WO2003078577A8 publication Critical patent/WO2003078577A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
PCT/US2003/007430 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana WO2003078577A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP2003576572A JP2005531297A (en) 2002-03-12 2003-03-12 Cloning of cytochrome P450 gene from tobacco
CA002477452A CA2477452A1 (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana
KR10-2004-7014250A KR20040091124A (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana
EP03716460A EP1572911A4 (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana
MXPA04008785A MXPA04008785A (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana.
BRPI0308351-9A BR0308351A (en) 2002-03-12 2003-03-12 isolated nucleic acid molecule, transgenic plant, and methods for producing a transgenic plant and for selecting a plant containing a nucleic acid molecule
APAP/P/2004/003122A AP2004003122A0 (en) 2002-03-12 2003-03-12 Cloning of cytochrome P450 genes from nicotiana
AU2003220165A AU2003220165A1 (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36368402P 2002-03-12 2002-03-12
US60/363,684 2002-03-12

Publications (3)

Publication Number Publication Date
WO2003078577A2 WO2003078577A2 (en) 2003-09-25
WO2003078577A9 WO2003078577A9 (en) 2004-01-22
WO2003078577A8 true WO2003078577A8 (en) 2008-01-10

Family

ID=28041798

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/007430 WO2003078577A2 (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana

Country Status (10)

Country Link
EP (1) EP1572911A4 (en)
JP (2) JP2005531297A (en)
KR (1) KR20040091124A (en)
CN (1) CN101166824A (en)
AP (1) AP2004003122A0 (en)
AU (1) AU2003220165A1 (en)
BR (1) BR0308351A (en)
CA (1) CA2477452A1 (en)
MX (1) MXPA04008785A (en)
WO (1) WO2003078577A2 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7700851B2 (en) 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
JP4519137B2 (en) * 2003-10-16 2010-08-04 ユーエス スモークレス タバコ カンパニー Cloning of cytochrome p450 gene from Nicotiana
CN1867674B (en) 2003-10-16 2011-11-09 美国无烟烟草有限责任公司 Cloning of cytochrome P450 genes from nicotiana
JP5422099B2 (en) * 2003-10-16 2014-02-19 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Cloning of cytochrome p450 gene from Nicotiana
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
ES2373881T3 (en) * 2004-04-29 2012-02-09 U.S. Smokeless Tobacco Company Llc GENOMIC CLONY OF TOBACCO NICOTINE DESMETILASE AND USES OF THE SAME.
WO2005111217A2 (en) * 2004-04-29 2005-11-24 U.S. Smokeless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
JP5106103B2 (en) * 2004-04-29 2012-12-26 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Tobacco nicotine demethylase genomic clone and its use
CN101384710B (en) * 2004-04-29 2012-05-02 美国无烟烟草有限责任公司 Nicotine demethylase genenome clone and uses thereof
EP2933328A1 (en) * 2004-04-29 2015-10-21 U.S. Smokeless Tobacco Company LLC Nicotiana nucleic acid molecules and uses thereof
AU2011232795B2 (en) * 2004-04-29 2015-05-28 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
JP4753937B2 (en) * 2004-04-29 2011-08-24 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Nicotiana nucleic acid molecule and use thereof
BRPI0519985B1 (en) 2005-02-23 2018-01-30 University Of Kentucky Research Foundation METHOD FOR REDUCING THE LEVEL OF NORNICOTIN OR N'-NITROSONORNICOTIN IN A NICOTIAN GENDER PLANT OR PART OF THIS OR IN A TOBACCO PRODUCT, EXPRESSION CASSETTE AND METHOD FOR THE PRODUCTION OF A NICOTIAN GENDER PLANT
DK2094841T3 (en) * 2006-12-13 2012-05-29 Dsm Sinochem Pharm Nl Bv Process for the preparation of pravastatin
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
CN101939431B (en) 2007-11-12 2015-01-28 北卡罗来纳州立大学 Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
EP2524044A1 (en) 2010-01-15 2012-11-21 North Carolina State University Compositions and methods for minimizing nornicotine synthesis in tobacco
RU2013139871A (en) * 2011-02-28 2015-04-10 Норт Каролина Стейт Юниверсити INBREED TOBACCO PLANTS NCBEX1F, NCBEX1MC AND NC EX90
JP2014511184A (en) 2011-02-28 2014-05-15 アルトリア クライアント サービシーズ インコーポレイテッド Tobacco inbred plants ALBEX1F and ALBEX1MS
CN105517430A (en) 2013-01-11 2016-04-20 北卡罗莱纳州立大学 Tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC AND HYBRID NC196 SRC
EP2964016A1 (en) 2013-03-05 2016-01-13 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
EP3113602A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
US9596824B2 (en) 2014-03-03 2017-03-21 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
EP3113603A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
KR102201082B1 (en) 2018-10-30 2021-01-08 인하대학교 산학협력단 The method of generating oxygen vacancies in nickel-cobalt oxide for oxygen reduction reaction and nickel-cobalt oxide thereby

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1373476B1 (en) * 2001-03-09 2011-06-15 University Of Kentucky Research Foundation Cytochrome p450s and uses thereof
EP2336305A3 (en) * 2002-10-16 2011-10-05 U.S. Smokeless Tobacco Company LLC Cloning of cytochrome P450 genes from Nicotiana

Also Published As

Publication number Publication date
AP2004003122A0 (en) 2004-09-30
JP2010131027A (en) 2010-06-17
BR0308351A (en) 2007-04-17
JP2005531297A (en) 2005-10-20
AU2003220165A8 (en) 2008-03-06
KR20040091124A (en) 2004-10-27
CN101166824A (en) 2008-04-23
EP1572911A2 (en) 2005-09-14
WO2003078577A2 (en) 2003-09-25
MXPA04008785A (en) 2005-09-30
CA2477452A1 (en) 2003-09-25
WO2003078577A9 (en) 2004-01-22
AU2003220165A1 (en) 2003-09-29
EP1572911A4 (en) 2008-06-25

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