WO2003050556A3 - Assay device and method - Google Patents

Assay device and method Download PDF

Info

Publication number
WO2003050556A3
WO2003050556A3 PCT/GB2002/005644 GB0205644W WO03050556A3 WO 2003050556 A3 WO2003050556 A3 WO 2003050556A3 GB 0205644 W GB0205644 W GB 0205644W WO 03050556 A3 WO03050556 A3 WO 03050556A3
Authority
WO
WIPO (PCT)
Prior art keywords
binding partner
detection zone
specific binding
solid support
sample
Prior art date
Application number
PCT/GB2002/005644
Other languages
French (fr)
Other versions
WO2003050556A2 (en
Inventor
Michael Frederick Sanders
Original Assignee
Sec Dep For Environment Food &
Michael Frederick Sanders
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sec Dep For Environment Food &, Michael Frederick Sanders filed Critical Sec Dep For Environment Food &
Priority to AU2002350951A priority Critical patent/AU2002350951A1/en
Publication of WO2003050556A2 publication Critical patent/WO2003050556A2/en
Publication of WO2003050556A3 publication Critical patent/WO2003050556A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for detecting the presence of a target moiety in a sample, said method comprising: (a) immersing in a liquid sample suspected of containing said moiety, a detection zone on a solid support, said detection zone having a first specific binding partner, which specifically binds said target moiety, immobilised therein, said first specific binding partner being present in the detection zone an amount which binds up to a predetermined concentration of said target moiety, (b) removing said solid support from the sample, (c) subsequently immersing the detection zone on the solid support in a solution or suspension of a labelled second specific binding partner which binds said first specific binding partner, wherein the amount of said labelled second specific binding partner is at least sufficient to bind to all said first binding partner, (d) detecting the presence of labelled material in the detection zone on said solid support; and (e) relating the result of (d) to the presence of target moiety in said sample. The method is particularly useful as a rapid test for pathogenic organisms such as anthrax spores, which may be present in high concentrations.
PCT/GB2002/005644 2001-12-13 2002-12-13 Assay device and method WO2003050556A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002350951A AU2002350951A1 (en) 2001-12-13 2002-12-13 Assay device and method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0129776A GB0129776D0 (en) 2001-12-13 2001-12-13 Assay device and method
GB0129776.1 2001-12-13

Publications (2)

Publication Number Publication Date
WO2003050556A2 WO2003050556A2 (en) 2003-06-19
WO2003050556A3 true WO2003050556A3 (en) 2003-10-16

Family

ID=9927520

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/005644 WO2003050556A2 (en) 2001-12-13 2002-12-13 Assay device and method

Country Status (3)

Country Link
AU (1) AU2002350951A1 (en)
GB (2) GB0129776D0 (en)
WO (1) WO2003050556A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7179596B2 (en) * 2003-02-17 2007-02-20 Sceptor Industries, Inc. Biological particulate matter analogue
WO2013016449A2 (en) * 2011-07-26 2013-01-31 Indicator Systems International, Inc. Assays for the detection of microbes
EP2796859A1 (en) * 2013-04-25 2014-10-29 Biocartis SA Quantification of a functionalized surface
CN111735954A (en) * 2020-06-23 2020-10-02 南京农业大学 Rapid immunity detection test strip for erwinia amylovora and application thereof

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4279885A (en) * 1978-05-19 1981-07-21 Becton Dickinson & Company Solid phase assay
US4391795A (en) * 1980-03-21 1983-07-05 Becton Dickinson And Company Assay for free thyroid hormone
EP0267317A1 (en) * 1983-05-20 1988-05-18 Profile Diagnostic Sciences Inc. Method for the detection of proteins and viruses
WO1988008978A1 (en) * 1987-05-14 1988-11-17 The Mclean Hospital Corporation Multiple antigen immunoassay
EP0311492A2 (en) * 1987-09-30 1989-04-12 Elf Sanofi Kit and immunoassay method applicable to whole cells
WO1990015327A1 (en) * 1989-06-06 1990-12-13 Martin Gould Immunoassay diagnostic kit
EP0577092A2 (en) * 1992-07-02 1994-01-05 Becton, Dickinson and Company Immunoassays using microparticles containing different detectable substances
WO2001049823A2 (en) * 2000-01-06 2001-07-12 Biosite Diagnostics, Inc. Assays for detection of bacillus anthracis
WO2001083561A2 (en) * 2000-04-28 2001-11-08 Tetracore, L.L.C. Anthrax-specific antigen, vaccines comprising said antigen, anthrax-specific antibodies, and their uses

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6479986A (en) * 1985-10-22 1987-05-19 Murex Corp. Idiotypic-antigenic conjunction binding assay
US6316205B1 (en) * 2000-01-28 2001-11-13 Genelabs Diagnostics Pte Ltd. Assay devices and methods of analyte detection

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4279885A (en) * 1978-05-19 1981-07-21 Becton Dickinson & Company Solid phase assay
US4391795A (en) * 1980-03-21 1983-07-05 Becton Dickinson And Company Assay for free thyroid hormone
EP0267317A1 (en) * 1983-05-20 1988-05-18 Profile Diagnostic Sciences Inc. Method for the detection of proteins and viruses
WO1988008978A1 (en) * 1987-05-14 1988-11-17 The Mclean Hospital Corporation Multiple antigen immunoassay
EP0311492A2 (en) * 1987-09-30 1989-04-12 Elf Sanofi Kit and immunoassay method applicable to whole cells
WO1990015327A1 (en) * 1989-06-06 1990-12-13 Martin Gould Immunoassay diagnostic kit
EP0577092A2 (en) * 1992-07-02 1994-01-05 Becton, Dickinson and Company Immunoassays using microparticles containing different detectable substances
WO2001049823A2 (en) * 2000-01-06 2001-07-12 Biosite Diagnostics, Inc. Assays for detection of bacillus anthracis
WO2001083561A2 (en) * 2000-04-28 2001-11-08 Tetracore, L.L.C. Anthrax-specific antigen, vaccines comprising said antigen, anthrax-specific antibodies, and their uses

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PHILIPS A P ET AL: "MONOCLONAL ANTIBODIES AGAINST SPORE ANTIGENS OF BACILLUS ANTHRACIS", FEMS MICROBIOLOGY IMMUNOLOGY, ELSEVIER, GB, vol. 47, no. 3, December 1988 (1988-12-01), pages 169 - 178, XP001031197, ISSN: 0920-8534 *
PHILLIPS A P ET AL: "EVALUATION OF IMMUNO RADIOMETRIC AND ELISA ENZYME LINKED IMMUNO SORBENT ASSAY VERSIONS OF A MICRO TITER PLATE ASSAY FOR BACILLUS-ANTHRACIS SPORES", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 70, no. 1, 1984, pages 75 - 82, XP009016145, ISSN: 0022-1759 *

Also Published As

Publication number Publication date
GB2385123A (en) 2003-08-13
WO2003050556A2 (en) 2003-06-19
GB0129776D0 (en) 2002-01-30
GB2385123B (en) 2004-05-05
AU2002350951A1 (en) 2003-06-23
GB0229038D0 (en) 2003-01-15

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