WO2003018792A1 - Procede de detection d'une substance toxique - Google Patents

Procede de detection d'une substance toxique Download PDF

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Publication number
WO2003018792A1
WO2003018792A1 PCT/JP2002/008495 JP0208495W WO03018792A1 WO 2003018792 A1 WO2003018792 A1 WO 2003018792A1 JP 0208495 W JP0208495 W JP 0208495W WO 03018792 A1 WO03018792 A1 WO 03018792A1
Authority
WO
WIPO (PCT)
Prior art keywords
toxic substance
detecting
test sample
detecting toxic
marker protein
Prior art date
Application number
PCT/JP2002/008495
Other languages
English (en)
Japanese (ja)
Inventor
Hitoshi Iwahashi
Yuko Momose
Emiko Kitagawa
Junko Takahashi
Original Assignee
National Institute Of Advanced Industrial Science And Technology
Daikin Industries, Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institute Of Advanced Industrial Science And Technology, Daikin Industries, Ltd. filed Critical National Institute Of Advanced Industrial Science And Technology
Priority to US10/487,438 priority Critical patent/US20050112573A1/en
Priority to JP2003523641A priority patent/JP4836403B2/ja
Priority to EP02760723A priority patent/EP1426439A4/fr
Publication of WO2003018792A1 publication Critical patent/WO2003018792A1/fr
Priority to US11/758,288 priority patent/US7704692B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention se rapporte à un procédé de détection d'une substance toxique au moyen d'un procédé biologique. Plus exactement, un composé toxique dans un échantillon pour essai est détecté par la transformation de cellules au moyen d'un vecteur contenant un polynucléotide, un polynucléotide codant une protéine de marquage étant ligaturé au promoteur d'un gène de levure spécifique. Le procédé consiste aussi à mettre l'échantillon pour essai en contact avec les cellules transformées puis à détecter l'expression d'ARN messager codant la protéine de marquage.
PCT/JP2002/008495 2001-08-24 2002-08-23 Procede de detection d'une substance toxique WO2003018792A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US10/487,438 US20050112573A1 (en) 2001-08-24 2002-08-23 Method of detecting toxic substance
JP2003523641A JP4836403B2 (ja) 2001-08-24 2002-08-23 毒性物質の検出方法
EP02760723A EP1426439A4 (fr) 2001-08-24 2002-08-23 Procede de detection d'une substance toxique
US11/758,288 US7704692B1 (en) 2001-08-24 2007-06-05 Process for detecting toxic substances

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001-255379 2001-08-24
JP2001255379 2001-08-24

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10487438 A-371-Of-International 2002-08-23
US11/758,288 Division US7704692B1 (en) 2001-08-24 2007-06-05 Process for detecting toxic substances

Publications (1)

Publication Number Publication Date
WO2003018792A1 true WO2003018792A1 (fr) 2003-03-06

Family

ID=19083355

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/008495 WO2003018792A1 (fr) 2001-08-24 2002-08-23 Procede de detection d'une substance toxique

Country Status (5)

Country Link
US (1) US20050112573A1 (fr)
EP (2) EP1921157B1 (fr)
JP (2) JP4836403B2 (fr)
DE (1) DE60236559D1 (fr)
WO (1) WO2003018792A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005054511A1 (fr) * 2003-12-02 2005-06-16 Daikin Industries, Ltd. Methode d'analyse d'agent chimique a l'aide d'une souche genetique disloquee
WO2006013859A1 (fr) * 2004-08-06 2006-02-09 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Promoteur de levure
WO2007139089A1 (fr) * 2006-05-29 2007-12-06 Daikin Industries, Ltd. Procédé amélioré pour déterminer un composé chimique
JP2009039132A (ja) * 2008-10-14 2009-02-26 Daikin Ind Ltd 改良された化学物質の検出方法
JP2009089709A (ja) * 2001-08-24 2009-04-30 National Institute Of Advanced Industrial & Technology 毒性物質の検出方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7872115B2 (en) * 2004-09-15 2011-01-18 Schering Corporation Reporter assay screens for protein targets in Saccharomyces cerevisiae
CN108359616B (zh) * 2018-02-14 2020-11-03 天津大学 Yel013w基因和yer042w基因的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000058520A1 (fr) * 1999-03-31 2000-10-05 Rosetta Inpharmatics, Inc. Procedes d'identification de rapporteurs specifiques des voies et genes cibles, et leurs utilisations
JP2001286281A (ja) * 2000-04-07 2001-10-16 Natl Inst Of Advanced Industrial Science & Technology Meti 化学物質の毒性評価方法及び同定方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITTO970751A1 (it) * 1997-08-20 1999-02-22 Giorgio Mangiarotti Uso del dictyostelium discoideum geneticamente ingegnerizzato e non per la rilevazione e l'individuazione di specifiche sostanze tossiche
AU1166199A (en) * 1997-11-20 1999-06-15 Smithkline Beecham Corporation Methods for identifying the toxic/pathologic effect of environmental stimuli on gene transcription
EP1108067A2 (fr) * 1998-08-28 2001-06-20 Incyte Pharmaceuticals, Inc. Marqueurs de reaction toxicologique
FR2798392B1 (fr) * 1999-09-13 2005-07-15 Exonhit Therapeutics Sa Marqueurs genetiques de la toxicite, preparation et utilisations
EP1921157B1 (fr) * 2001-08-24 2010-05-26 Daikin Industries, Ltd. Processus de détection de substances toxiques

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000058520A1 (fr) * 1999-03-31 2000-10-05 Rosetta Inpharmatics, Inc. Procedes d'identification de rapporteurs specifiques des voies et genes cibles, et leurs utilisations
JP2001286281A (ja) * 2000-04-07 2001-10-16 Natl Inst Of Advanced Industrial Science & Technology Meti 化学物質の毒性評価方法及び同定方法

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
ALBERTS B. ET AL.: "Essential cell biology", 1998, GARLAND PUBLISHING, INC., NEW YORK, pages: 323, XP002960461 *
BELLI G. ET AL.: "Functional analysis of yeast essential genes using a promoter-substitution cassette and the tetracycline-regulatable dual expression system", YEAST, vol. 14, no. 12, 1998, pages 1127 - 1138, XP000939006 *
CASALONE E. ET AL.: "Disruption and phenotypic analysis of six novel genes from chromosome IV of saccharomyces cerevisiae reveal YDL060w as an essential gene for vegetative growth", YEAST, vol. 15, no. 15, 1999, pages 1691 - 1701, XP002960459 *
FUJITA K. ET AL.: "Hsp104 expression and morphological changes associated with disinfectants in saccharomyces cerevisiae: Environmental bioassay using stress response", WATER SCIENCE AND TECHNOLOGY, vol. 38, no. 7, 1998, pages 237 - 243, XP002960456 *
HUANG M.E.: "Dusruption of six novel yeast genes reveals three genes essential for vegetative growth and one required for growth at low temperature", YEAST, vol. 13, no. 12, 1997, pages 1181 - 1194, XP001021576 *
LASHKARI D.A. ET AL.: "Yeast microarrays for genome wide parallel genetic and gene expression analysis", PROC. NATL. ACAD. SCI. USA, vol. 94, 1997, pages 13057 - 13062, XP002115013 *
MIURA S. ET AL.: "Screening of genes involved in isooctane tolerance in saccharomyces cerevisiae by using mRNA differential display", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 11, 2000, pages 4883 - 4889, XP002960457 *
PARRY J.M.: "The use of yeast cultures for the detection of environmental mutagens using a fluctuation test", MUTATION RESEARCH, vol. 46, no. 3, 1977, pages 165 - 175, XP002960458 *
PREIN B. ET AL.: "A novel strategy for constructing N-terminal chromosomal fusions to green fluorescent protein in the yeast saccharomyces cerevisiae", FEBS LETTERS, vol. 485, no. 1, 2000, pages 29 - 34, XP004337764 *
SARTORI G. ET AL.: "Inactivation of six genes from chromosomes VII and XIV of saccharomyces cerevisiae and basic phenotypic analysis of the mutant strains", YEAST, vol. 16, no. 3, 2000, pages 255 - 265, XP002960460 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009089709A (ja) * 2001-08-24 2009-04-30 National Institute Of Advanced Industrial & Technology 毒性物質の検出方法
WO2005054511A1 (fr) * 2003-12-02 2005-06-16 Daikin Industries, Ltd. Methode d'analyse d'agent chimique a l'aide d'une souche genetique disloquee
US8173387B2 (en) 2003-12-02 2012-05-08 Daikin Industires, Ltd. Method of examining chemical using gene-disrupted strain
WO2006013859A1 (fr) * 2004-08-06 2006-02-09 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Promoteur de levure
JPWO2006013859A1 (ja) * 2004-08-06 2008-05-01 財団法人化学及血清療法研究所 酵母プロモーター
JP4819683B2 (ja) * 2004-08-06 2011-11-24 一般財団法人化学及血清療法研究所 酵母プロモーター
WO2007139089A1 (fr) * 2006-05-29 2007-12-06 Daikin Industries, Ltd. Procédé amélioré pour déterminer un composé chimique
JP2009039132A (ja) * 2008-10-14 2009-02-26 Daikin Ind Ltd 改良された化学物質の検出方法

Also Published As

Publication number Publication date
EP1921157B1 (fr) 2010-05-26
EP1921157A3 (fr) 2008-05-21
JP4836403B2 (ja) 2011-12-14
DE60236559D1 (de) 2010-07-08
EP1921157A2 (fr) 2008-05-14
JP5071807B2 (ja) 2012-11-14
JP2009089709A (ja) 2009-04-30
JPWO2003018792A1 (ja) 2004-12-09
EP1426439A4 (fr) 2005-08-10
US20050112573A1 (en) 2005-05-26
EP1426439A1 (fr) 2004-06-09

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