WO2003014398A3 - Methods and compositions for mutation detection by liquid chromatography - Google Patents
Methods and compositions for mutation detection by liquid chromatography Download PDFInfo
- Publication number
- WO2003014398A3 WO2003014398A3 PCT/US2001/045676 US0145676W WO03014398A3 WO 2003014398 A3 WO2003014398 A3 WO 2003014398A3 US 0145676 W US0145676 W US 0145676W WO 03014398 A3 WO03014398 A3 WO 03014398A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- dna
- hybridization
- mutation detection
- compositions
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25984701P | 2001-01-03 | 2001-01-03 | |
US60/259,847 | 2001-01-03 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003014398A2 WO2003014398A2 (en) | 2003-02-20 |
WO2003014398A3 true WO2003014398A3 (en) | 2003-07-10 |
Family
ID=22986670
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/045676 WO2003014398A2 (en) | 2001-01-03 | 2001-10-31 | Methods and compositions for mutation detection by liquid chromatography |
Country Status (1)
Country | Link |
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WO (1) | WO2003014398A2 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0428255D0 (en) | 2004-12-23 | 2005-01-26 | Health Prot Agency | Detection of nucleic acid mutations |
WO2009144581A1 (en) | 2008-05-27 | 2009-12-03 | Dako Denmark A/S | Hybridization compositions and methods |
EP2401395A1 (en) | 2009-02-26 | 2012-01-04 | Dako Denmark A/S | Compositions and methods for rna hybridization applications |
EP2761028A1 (en) | 2011-09-30 | 2014-08-06 | Dako Denmark A/S | Hybridization compositions and methods using formamide |
EP2768974B1 (en) | 2011-10-21 | 2017-07-19 | Dako Denmark A/S | Hybridization compositions and methods |
CN104204203A (en) | 2012-03-22 | 2014-12-10 | 和光纯药工业株式会社 | Method for detecting DNA having microsatellite region |
AU2013236175A1 (en) | 2012-03-22 | 2014-10-16 | Kanagawa Prefectural Hospital Organization | Method for identification and detection of mutant gene using intercalator |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4411588C1 (en) * | 1994-03-30 | 1995-09-28 | Deutsches Rheuma Forschungszen | Buffer, contg. betaine, for RNA- and DNA-polymerase reactions |
WO1997043450A1 (en) * | 1996-05-16 | 1997-11-20 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
US5795976A (en) * | 1995-08-08 | 1998-08-18 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of nucleic acid heteroduplex molecules by denaturing high-performance liquid chromatography and methods for comparative sequencing |
WO1999009217A1 (en) * | 1997-08-15 | 1999-02-25 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
EP0952228A2 (en) * | 1996-09-24 | 1999-10-27 | Rapigene, Inc. | Compositions and methods for enhancing hybridization specificity |
-
2001
- 2001-10-31 WO PCT/US2001/045676 patent/WO2003014398A2/en active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4411588C1 (en) * | 1994-03-30 | 1995-09-28 | Deutsches Rheuma Forschungszen | Buffer, contg. betaine, for RNA- and DNA-polymerase reactions |
US5795976A (en) * | 1995-08-08 | 1998-08-18 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of nucleic acid heteroduplex molecules by denaturing high-performance liquid chromatography and methods for comparative sequencing |
WO1997043450A1 (en) * | 1996-05-16 | 1997-11-20 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
EP0952228A2 (en) * | 1996-09-24 | 1999-10-27 | Rapigene, Inc. | Compositions and methods for enhancing hybridization specificity |
WO1999009217A1 (en) * | 1997-08-15 | 1999-02-25 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
Non-Patent Citations (15)
Title |
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CHAKRABARTI R ET AL: "The enhancement of PCR amplification by low molecular weight amides.", NUCLEIC ACIDS RESEARCH. ENGLAND 1 JUN 2001, vol. 29, no. 11, 1 June 2001 (2001-06-01), pages 2377 - 2381, XP002227234, ISSN: 1362-4962 * |
CHAKRABARTI R ET AL: "The enhancement of PCR amplification by low molecular-weight sulfones.", GENE. NETHERLANDS 22 AUG 2001, vol. 274, no. 1-2, 22 August 2001 (2001-08-22), pages 293 - 298, XP002227233, ISSN: 0378-1119 * |
DE SILVA D ET AL: "Monitoring hybridization during polymerase chain reaction.", JOURNAL OF CHROMATOGRAPHY. B, BIOMEDICAL SCIENCES AND APPLICATIONS. NETHERLANDS 28 APR 2000, vol. 741, no. 1, 28 April 2000 (2000-04-28), pages 3 - 13, XP002227231, ISSN: 1387-2273 * |
HENGEN P N: "Optimizing multiplex and LA-PCR with betaine", TIBS TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER PUBLICATION, CAMBRIDGE, EN, vol. 22, no. 6, 1 June 1997 (1997-06-01), pages 225 - 226, XP004074684, ISSN: 0968-0004 * |
HENKE W ET AL: "Recently, betaine has been introduced as an additive in different PCR strategies.", NUCLEIC ACIDS RESEARCH. ENGLAND 15 JAN 1998, vol. 26, no. 2, 15 January 1998 (1998-01-15), pages 687, XP001097595, ISSN: 0305-1048 * |
LIU W ET AL: "DENATURING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (DHPLC) USED IN THE DETECTION OF GERMLINE AND SOMATIC MUTATIONS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 6, 1998, pages 1396 - 1400, XP002911657, ISSN: 0305-1048 * |
MELCHIOR W B JR ET AL: "Alteration of the relative stability of dA-dT and dG-dC base pairs in DNA.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. UNITED STATES FEB 1973, vol. 70, no. 2, February 1973 (1973-02-01), pages 298 - 302, XP002027509, ISSN: 0027-8424 * |
MYERS R M ET AL: "MODIFICATION OF THE MELTING PROPERTIES OF DUPLEX DNA BY ATTACHMENT OF A GC-RICH DNA SEQUENCE AS DETERMINED BY DENATURING GRADIENT GEL ELECTROPHORESIS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 13, no. 9, 1985, pages 3111 - 3129, XP002911668, ISSN: 0305-1048 * |
MYERS R M ET AL: "Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis.", NUCLEIC ACIDS RESEARCH. ENGLAND 10 MAY 1985, vol. 13, no. 9, 10 May 1985 (1985-05-10), pages 3131 - 3145, XP009004065, ISSN: 0305-1048 * |
NARAYANASWAMI G ET AL: "Improved efficiency of mutation detection by denaturing high-performance liquid chromatography using modified primers and hybridization procedure.", GENETIC TESTING. UNITED STATES 2001 SPRING, vol. 5, no. 1, April 2001 (2001-04-01), pages 9 - 16, XP009003995, ISSN: 1090-6576 * |
OEFNER P J: "Allelic discrimination by denaturing high-performance liquid chromatography", JOURNAL OF CHROMATOGRAPHY. BIOMEDICAL APPLICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 739, no. 2, March 2000 (2000-03-01), pages 345 - 355, XP004202696, ISSN: 0378-4347 * |
REES W A ET AL: "BETAINE CAN ELIMINATE THE BASE PAIR COMPOSITION DEPENDENCE OF DNA MELTING", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 32, no. 1, 1 December 1993 (1993-12-01), pages 137 - 144, XP000602183, ISSN: 0006-2960 * |
SKOPEK T R ET AL: "Analysis of sequence alterations in a defined DNA region: comparison of temperature-modulated heteroduplex analysis and denaturing gradient gel electrophoresis.", MUTATION RESEARCH. NETHERLANDS 29 NOV 1999, vol. 430, no. 1, 29 November 1999 (1999-11-29), pages 13 - 21, XP002227232, ISSN: 0027-5107 * |
WOOD W I ET AL: "BASE COMPOSITION-INDEPENDENT HYBRIDIZATION IN TETRAMETHYLAMMONIUM CHLORIDE: A METHOD FOR OLIGONUCLEOTIDE SCREENING OF HIGHLY COMPLEX GENE LIBRARIES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 82, no. 3, 1 March 1985 (1985-03-01), pages 1585 - 1588, XP002027508, ISSN: 0027-8424 * |
WU Y ET AL: "Improvement of fragment and primer selection for mutation detection by denaturing gradient gel electrophoresis.", NUCLEIC ACIDS RESEARCH. ENGLAND 1 DEC 1998, vol. 26, no. 23, 1 December 1998 (1998-12-01), pages 5432 - 5440, XP002227230, ISSN: 0305-1048 * |
Also Published As
Publication number | Publication date |
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WO2003014398A2 (en) | 2003-02-20 |
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