WO2003008635A8 - η-SECRETASE IN VITRO SCREENING ASSAY - Google Patents

η-SECRETASE IN VITRO SCREENING ASSAY

Info

Publication number
WO2003008635A8
WO2003008635A8 PCT/EP2002/007095 EP0207095W WO03008635A8 WO 2003008635 A8 WO2003008635 A8 WO 2003008635A8 EP 0207095 W EP0207095 W EP 0207095W WO 03008635 A8 WO03008635 A8 WO 03008635A8
Authority
WO
WIPO (PCT)
Prior art keywords
secretase
screening assay
vitro screening
substrate
secretase activity
Prior art date
Application number
PCT/EP2002/007095
Other languages
French (fr)
Other versions
WO2003008635A3 (en
WO2003008635A2 (en
Inventor
Cornelia Dorner-Ciossek
Klaus Fuchs
Christian Haass
Markus Kostka
Harald Steiner
Original Assignee
Boehringer Ingelheim Pharma
Cornelia Dorner-Ciossek
Klaus Fuchs
Christian Haass
Markus Kostka
Harald Steiner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Pharma, Cornelia Dorner-Ciossek, Klaus Fuchs, Christian Haass, Markus Kostka, Harald Steiner filed Critical Boehringer Ingelheim Pharma
Priority to MXPA03011898A priority Critical patent/MXPA03011898A/en
Priority to CA002452832A priority patent/CA2452832A1/en
Priority to EP02745414A priority patent/EP1407043A2/en
Priority to JP2003514950A priority patent/JP2004535206A/en
Publication of WO2003008635A2 publication Critical patent/WO2003008635A2/en
Publication of WO2003008635A8 publication Critical patent/WO2003008635A8/en
Publication of WO2003008635A3 publication Critical patent/WO2003008635A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Psychiatry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

According to the screening method of the invention substances capable of modulating η-secretase activity are identified by incubating a source and a substrate of η-secretase activity with a test substance and detecting a C-terminal fragment released from said substrate.
PCT/EP2002/007095 2001-07-04 2002-06-27 η-SECRETASE IN VITRO SCREENING ASSAY WO2003008635A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
MXPA03011898A MXPA03011898A (en) 2001-07-04 2002-06-27 gamma-SECRETASE IN VITRO.
CA002452832A CA2452832A1 (en) 2001-07-04 2002-06-27 .gamma.-secretase in vitro screening assay
EP02745414A EP1407043A2 (en) 2001-07-04 2002-06-27 Gamma-secretase in vitro screening assay
JP2003514950A JP2004535206A (en) 2001-07-04 2002-06-27 In vitro screening assay for γ-secretase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10131899A DE10131899A1 (en) 2001-07-04 2001-07-04 In Vitro Screening Assay for Gamma Secretase
DE10131899.5 2001-07-04

Publications (3)

Publication Number Publication Date
WO2003008635A2 WO2003008635A2 (en) 2003-01-30
WO2003008635A8 true WO2003008635A8 (en) 2003-10-30
WO2003008635A3 WO2003008635A3 (en) 2004-01-29

Family

ID=7690254

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/007095 WO2003008635A2 (en) 2001-07-04 2002-06-27 η-SECRETASE IN VITRO SCREENING ASSAY

Country Status (6)

Country Link
EP (1) EP1407043A2 (en)
JP (1) JP2004535206A (en)
CA (1) CA2452832A1 (en)
DE (1) DE10131899A1 (en)
MX (1) MXPA03011898A (en)
WO (1) WO2003008635A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0229582D0 (en) * 2002-12-19 2003-01-22 Merck Sharp & Dohme Novel assay
PT1849762E (en) 2006-04-21 2009-09-16 Cellzome Ltd Substituted biphenyl carboxylic acids and derivatives thereof
CN101679326B (en) 2007-03-07 2012-12-12 詹森药业有限公司 Substituted phenoxy n-alkylated thiazoledinedione as estrogen related receptor-alpha modulators
KR20090128454A (en) 2007-03-07 2009-12-15 얀센 파마슈티카 엔.브이. Substituted phenoxy aminothiazolones as estrogen related receptor-alpha modulators
EP2183598A1 (en) * 2007-07-13 2010-05-12 Elan Pharmaceuticals Inc. Compositions and methods for identifying substrate specificity of inhibitors of gamma secretase
CN101861297B (en) 2007-10-17 2014-04-23 詹森药业有限公司 Biphenyl carboxylic acids and derivatives thereof
AU2008312653B2 (en) 2007-10-19 2013-03-07 Janssen Pharmaceutica, N.V. Amide linked modulators of y-secretase
EA017119B1 (en) 2007-10-19 2012-09-28 Янссен Фармацевтика, Н.В. PIPERIDINYL AND PIPERAZINYL MODULATORS OF γ-SECRETASE
EP2215043B1 (en) 2007-10-19 2013-03-06 Janssen Pharmaceutica, N.V. Carbon linked modulators of y-secretase
JP5543354B2 (en) 2007-10-19 2014-07-09 ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ Gamma-secretase amine binding regulator
US7968725B2 (en) 2008-07-22 2011-06-28 Janssen Pharmaceutica N.V. Pyridinyl modulators of γ-secretase
US9023767B2 (en) 2009-05-07 2015-05-05 Memorial Sloan-Kettering Cancer Center γ-Secretase substrates and methods of use
CN103502466A (en) * 2010-09-07 2014-01-08 斯隆－凯特林纪念癌症中心 Methods and compositions for gamma-secretase assay
WO2014062771A1 (en) * 2012-10-16 2014-04-24 The Johns Hopkins University An inducible reconstitution and real-time quantitative kinetic system for the study of intramembrane enzymes

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998015828A1 (en) * 1996-10-07 1998-04-16 Scios Inc. Method to identify direct inhibitors of the beta-amyloid forming enzyme gamma-secretase
DE19941039A1 (en) * 1999-08-28 2001-03-01 Boehringer Ingelheim Pharma gamma-secretase in vitro test system

Also Published As

Publication number Publication date
WO2003008635A3 (en) 2004-01-29
DE10131899A1 (en) 2003-02-27
JP2004535206A (en) 2004-11-25
CA2452832A1 (en) 2003-01-30
EP1407043A2 (en) 2004-04-14
MXPA03011898A (en) 2004-03-26
WO2003008635A2 (en) 2003-01-30

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Free format text: IN PCT GAZETTE 05/2003 DELETE "(72) INVENTOR (FOR US ONLY): HAASS, CHRISTIAN; ICHORING 29 A, 82057ICKING (DE)." AND UNDER (72, 75) ADD "HAASS, CHRISTIAN [DE/DE]; ICHORING 29 A, 82057 ICKING (DE)."

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