WO2002101339A3 - Imaging and analyzing parameters of small moving objects such as cells - Google Patents

Imaging and analyzing parameters of small moving objects such as cells Download PDF

Info

Publication number
WO2002101339A3
WO2002101339A3 PCT/US2001/042706 US0142706W WO02101339A3 WO 2002101339 A3 WO2002101339 A3 WO 2002101339A3 US 0142706 W US0142706 W US 0142706W WO 02101339 A3 WO02101339 A3 WO 02101339A3
Authority
WO
WIPO (PCT)
Prior art keywords
light
imaging
detector
cells
moving objects
Prior art date
Application number
PCT/US2001/042706
Other languages
French (fr)
Other versions
WO2002101339A2 (en
Inventor
William Ortyn
David Basiji
Original Assignee
Amnis Corp
William Ortyn
David Basiji
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/820,434 external-priority patent/US6473176B2/en
Application filed by Amnis Corp, William Ortyn, David Basiji filed Critical Amnis Corp
Priority to EP01274071A priority Critical patent/EP1334338A4/en
Priority to JP2003504054A priority patent/JP4018063B2/en
Priority to AU2001297843A priority patent/AU2001297843A1/en
Publication of WO2002101339A2 publication Critical patent/WO2002101339A2/en
Publication of WO2002101339A3 publication Critical patent/WO2002101339A3/en

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/14—Beam splitting or combining systems operating by reflection only
    • G02B27/148—Beam splitting or combining systems operating by reflection only including stacked surfaces having at least one double-pass partially reflecting surface
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0208—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/021—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using plane or convex mirrors, parallel phase plates, or particular reflectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2803—Investigating the spectrum using photoelectric array detector
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/30—Measuring the intensity of spectral lines directly on the spectrum itself
    • G01J3/36—Investigating two or more bands of a spectrum by separate detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/0012—Optical design, e.g. procedures, algorithms, optimisation routines
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/1006—Beam splitting or combining systems for splitting or combining different wavelengths
    • G02B27/1013—Beam splitting or combining systems for splitting or combining different wavelengths for colour or multispectral image sensors, e.g. splitting an image into monochromatic image components on respective sensors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/12—Beam splitting or combining systems operating by refraction only
    • G02B27/126—The splitting element being a prism or prismatic array, including systems based on total internal reflection
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/14—Beam splitting or combining systems operating by reflection only
    • G02B27/144—Beam splitting or combining systems operating by reflection only using partially transparent surfaces without spectral selectivity
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/14—Beam splitting or combining systems operating by reflection only
    • G02B27/145—Beam splitting or combining systems operating by reflection only having sequential partially reflecting surfaces
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
    • G02B7/28—Systems for automatic generation of focusing signals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N2015/0294—Particle shape
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/144—Imaging characterised by its optical setup
    • G01N2015/1443—Auxiliary imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N2015/1472—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle with colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • G01N2015/1479—Using diffuse illumination or excitation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G01N2021/058—Flat flow cell

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Eye Examination Apparatus (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Light from an object such as a cell moving through an imaging system is collected and dispersed so that it is imaged onto a plurality of separate detectors (321-325). The light is spectrally dispersed by a plurality of spaced-apart dichroic reflectors (301-304), each detector (321-325) receiving light from a different one of the dichroic reflectors (301-304). Each dichroic filter (301-304) reflects light of a different predefined color, passing light of other colors. The output signal from each detector (321-325) is indicative of a different characteristic of the object. In one configuration, each detector (321-325) is provided with a separate imaging lens (311-315). In another configuration, the detectors (321-325) are spaced at varying distances from the corresponding dichroic reflectors (301-304), so that separate imaging lenses are not required.
PCT/US2001/042706 2000-10-12 2001-10-12 Imaging and analyzing parameters of small moving objects such as cells WO2002101339A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01274071A EP1334338A4 (en) 2000-10-12 2001-10-12 Imaging and analyzing parameters of small moving objects such as cells
JP2003504054A JP4018063B2 (en) 2000-10-12 2001-10-12 Imaging system and method
AU2001297843A AU2001297843A1 (en) 2000-10-12 2001-10-12 Imaging and analyzing parameters of small moving objects such as cells

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US24012500P 2000-10-12 2000-10-12
US60/240,125 2000-10-12
US09/820,434 2001-03-29
US09/820,434 US6473176B2 (en) 1999-01-25 2001-03-29 Imaging and analyzing parameters of small moving objects such as cells

Publications (2)

Publication Number Publication Date
WO2002101339A2 WO2002101339A2 (en) 2002-12-19
WO2002101339A3 true WO2002101339A3 (en) 2003-05-01

Family

ID=26933159

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/042706 WO2002101339A2 (en) 2000-10-12 2001-10-12 Imaging and analyzing parameters of small moving objects such as cells

Country Status (3)

Country Link
EP (1) EP1334338A4 (en)
JP (2) JP4018063B2 (en)
WO (1) WO2002101339A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103620378A (en) * 2011-04-21 2014-03-05 麻省理工学院 Highly compact multi-optical-junction optical flowcell and flexibly deployable optical sensing assemblies and systems for in-situ real-time spectroscopic measurements

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8885913B2 (en) 1999-01-25 2014-11-11 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8131053B2 (en) 1999-01-25 2012-03-06 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8406498B2 (en) 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
EP1828830A1 (en) 2004-12-21 2007-09-05 Cedars-Sinai Medical Center Method for drug screening and characterization by ion flux analysis
WO2006083969A2 (en) * 2005-02-01 2006-08-10 Amnis Corporation Blood analysis using a flow imaging cytometer
US7528951B2 (en) * 2006-03-23 2009-05-05 Hach Company Optical design of a measurement system having multiple sensor or multiple light source paths
US7805020B2 (en) * 2006-07-25 2010-09-28 Itt Manufacturing Enterprises, Inc. Motion compensated image registration for overlaid/fused video
EP2201352B2 (en) 2007-09-28 2018-08-29 Illumina, Inc. Fluorescence excitation and detection system and method
DE202008003977U1 (en) * 2008-02-26 2009-07-02 Bürkert Werke GmbH & Co. KG microspectrometer
CN101650313B (en) * 2009-09-25 2011-08-17 开物科技股份有限公司 Optical fast detection reagent analyzer
JP5869347B2 (en) * 2011-02-03 2016-02-24 Hoya株式会社 Transmittance measuring apparatus and transmittance measuring method
CA2841425C (en) * 2011-03-31 2018-05-01 Gnubio, Inc. Scalable spectroscopic detection and measurement
US8772731B2 (en) * 2012-04-15 2014-07-08 Kla-Tencor Corporation Apparatus and method for synchronizing sample stage motion with a time delay integration charge-couple device in a semiconductor inspection tool
US10816550B2 (en) 2012-10-15 2020-10-27 Nanocellect Biomedical, Inc. Systems, apparatus, and methods for sorting particles
US9513206B2 (en) 2013-03-29 2016-12-06 Sysmex Corporation Particle measuring apparatus
JP6628743B2 (en) * 2014-06-03 2020-01-15 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Nanoparticle analysis system and method
EP3187854B1 (en) 2014-08-28 2020-08-19 Sysmex Corporation Particle image-capturing device and particle image-capturing method
KR102258807B1 (en) * 2015-02-24 2021-06-09 (주)미디어에버 Detection apparatus for micro dust and organism
CN108369169B (en) * 2015-10-14 2021-10-26 堀场仪器株式会社 Device and method for measuring growth or dissolution kinetics of colloidal particles
EP3408643B1 (en) 2016-01-25 2021-12-01 Plair SA Method and device for detection and/or morphologic analysis of individual fluid-borne particles
US10585031B2 (en) * 2017-02-27 2020-03-10 Becton, Dickinson And Company Light detection systems and methods for using thereof
DE102017119284B4 (en) 2017-08-23 2023-03-30 Markus Klotz particle sensor
CN110927126A (en) * 2018-09-20 2020-03-27 牛尾电机(苏州)有限公司 Fluorescence measurement container and fluorescence measurement device
JP7233531B2 (en) * 2018-10-25 2023-03-06 プレアー ソシエテ・アノニム Method and apparatus for detecting and/or measuring impurities in droplets
US11281902B1 (en) 2019-06-19 2022-03-22 Imaging Business Machines Llc Document scanning system

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6211955B1 (en) * 2000-01-24 2001-04-03 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4777525A (en) * 1985-12-23 1988-10-11 Preston Jr Kendall Apparatus and method for a multi-resolution electro-optical imaging, display and storage/retrieval system
JPH02181632A (en) * 1989-01-06 1990-07-16 Hitachi Ltd Sheath flow cell
DE69229777T3 (en) * 1991-04-10 2010-12-30 Mayo Foundation For Medical Education And Research, Rochester CONFOCUS PICTURE SYSTEM FOR VISIBLE AND UV LIGHT
JP3102935B2 (en) * 1991-11-20 2000-10-23 シスメックス株式会社 Imaging flow cytometer
CA2228308A1 (en) * 1995-08-01 1997-02-13 Medispectra, Inc. Optical microprobes and methods for spectral analysis of materials
JP3808169B2 (en) * 1997-05-23 2006-08-09 株式会社ルネサステクノロジ Inspection method and apparatus, and semiconductor substrate manufacturing method
US5900942A (en) * 1997-09-26 1999-05-04 The United States Of America As Represented By Administrator Of National Aeronautics And Space Administration Multi spectral imaging system
US6007775A (en) * 1997-09-26 1999-12-28 University Of Washington Multiple analyte diffusion based chemical sensor
CA2331897C (en) * 1998-05-14 2008-11-18 Luminex Corporation Multi-analyte diagnostic system and computer implemented process for same
FR2784189B3 (en) * 1998-10-05 2000-11-03 Commissariat Energie Atomique BIOCHIP AND DEVICE FOR READING A BIOCHIP COMPRISING A PLURALITY OF MOLECULAR RECOGNITION AREAS
US6256096B1 (en) * 1999-01-11 2001-07-03 Softray Flow cytometry apparatus and method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6211955B1 (en) * 2000-01-24 2001-04-03 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103620378A (en) * 2011-04-21 2014-03-05 麻省理工学院 Highly compact multi-optical-junction optical flowcell and flexibly deployable optical sensing assemblies and systems for in-situ real-time spectroscopic measurements

Also Published As

Publication number Publication date
JP5160158B2 (en) 2013-03-13
EP1334338A2 (en) 2003-08-13
EP1334338A4 (en) 2008-03-19
JP2007263983A (en) 2007-10-11
JP4018063B2 (en) 2007-12-05
WO2002101339A2 (en) 2002-12-19
JP2004522163A (en) 2004-07-22

Similar Documents

Publication Publication Date Title
WO2002101339A3 (en) Imaging and analyzing parameters of small moving objects such as cells
WO2005019873A3 (en) Optical elements related manufacturing methods and assemblies incorporating optical elements
US9420241B2 (en) Multi-spectral imaging
JP4799550B2 (en) Pupil detection method and system
US7746396B2 (en) Imaging device and method of creating image file
CN204963859U (en) Remote sensing parameter camera
JP2007515132A (en) Method and system for wavelength dependent imaging and detection using hybrid filters
US10101206B2 (en) Spectral imaging method and system
EP2309237A3 (en) Radiometry using an uncooled microbolometer detector
WO2005008198A3 (en) Method and apparatus for compact birefringent interference imaging spectrometer
CA2294840A1 (en) Multi-spectral two-dimensional imaging spectrometer
US20130208146A1 (en) Method and apparatus for separate spectral imaging and sensing
KR102100031B1 (en) Camera and method of producing color images
JP6547073B2 (en) Imager with improved autofocus performance
KR20120058597A (en) High spectral resolution color sensor using non-dispersive elements
US10859435B2 (en) Method and apparatus for detecting a laser
US20190058837A1 (en) System for capturing scene and nir relighting effects in movie postproduction transmission
GB2427912A (en) Imaging system for locating retroreflectors
JP2001008220A5 (en)
CN107389602A (en) A kind of spectroscopic system based on DLP technologies
US11696043B2 (en) White balance compensation using a spectral sensor system
CN113122614A (en) Fluorescent quantitative PCR processing method and system
JP2007235760A (en) Ultraviolet image, imaging apparatus for imaging ultraviolet image, and imaging method
US11015982B2 (en) Wavefront detector
EP3343897B1 (en) Camera and method of producing color images

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2003504054

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2001274071

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001274071

Country of ref document: EP