WO2002101057A1 - Transfert genique dans des cellules souches embryonnaires de primate a l'aide d'un virus de l'immunodeficience simienne de pseudo type vsv-g utilise comme vecteur - Google Patents

Transfert genique dans des cellules souches embryonnaires de primate a l'aide d'un virus de l'immunodeficience simienne de pseudo type vsv-g utilise comme vecteur Download PDF

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Publication number
WO2002101057A1
WO2002101057A1 PCT/JP2002/005225 JP0205225W WO02101057A1 WO 2002101057 A1 WO2002101057 A1 WO 2002101057A1 JP 0205225 W JP0205225 W JP 0205225W WO 02101057 A1 WO02101057 A1 WO 02101057A1
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WIPO (PCT)
Prior art keywords
stem cells
embryonic stem
vsv
gene transfer
immunodeficiency virus
Prior art date
Application number
PCT/JP2002/005225
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English (en)
French (fr)
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WO2002101057A8 (fr
Inventor
Yutaka Hanazono
Yasuji Ueda
Yasushi Kondo
Yutaka Suzuki
Original Assignee
Dnavec Research Inc.
Tanabe Seiyaku Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dnavec Research Inc., Tanabe Seiyaku Co., Ltd. filed Critical Dnavec Research Inc.
Priority to US10/479,912 priority Critical patent/US7323337B2/en
Priority to CA002449589A priority patent/CA2449589A1/en
Priority to EP02728191A priority patent/EP1403374A4/en
Priority to JP2003503807A priority patent/JPWO2002101057A1/ja
Priority to AU2002258248A priority patent/AU2002258248C1/en
Priority to KR1020037015930A priority patent/KR100682708B1/ko
Publication of WO2002101057A1 publication Critical patent/WO2002101057A1/ja
Publication of WO2002101057A8 publication Critical patent/WO2002101057A8/ja

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073Stem cells
    • CCHEMISTRY; METALLURGY
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/10Mammal
    • A01K2227/105Murine
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/10Mammal
    • A01K2227/106Primate
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/02Animal zootechnically ameliorated
    • A01K2267/025Animal producing cells or organs for transplantation
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/03Animal model, e.g. for test or diseases
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/15011Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/15011Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041Use of virus, viral particle or viral elements as a vector
    • C12N2740/15045Special targeting system for viral vectors
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    • C12N2810/00Vectors comprising a targeting moiety
    • C12N2810/50Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6072Vectors comprising as targeting moiety peptide derived from defined protein from viruses negative strand RNA viruses
    • C12N2810/6081Vectors comprising as targeting moiety peptide derived from defined protein from viruses negative strand RNA viruses rhabdoviridae, e.g. VSV
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/22Assays involving biological materials from specific organisms or of a specific nature from bacteria from Neisseriaceae (F), e.g. Acinetobacter

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  • Biotechnology (AREA)
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  • Cell Biology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
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  • Virology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PCT/JP2002/005225 2001-06-08 2002-05-29 Transfert genique dans des cellules souches embryonnaires de primate a l'aide d'un virus de l'immunodeficience simienne de pseudo type vsv-g utilise comme vecteur WO2002101057A1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US10/479,912 US7323337B2 (en) 2001-06-08 2002-05-29 Gene transfer into primate embryonic stem cells using VSV-G pseudotyped simian immunodeficiency virus vectors
CA002449589A CA2449589A1 (en) 2001-06-08 2002-05-29 Gene transfer into primate embryonic stem cells using vsv-g pseudotyped simian immunodeficiency virus vector
EP02728191A EP1403374A4 (en) 2001-06-08 2002-05-29 GENE TRANSFER IN EMBRYONIC PRIMATE STEM CELLS WITH THE VSV-G PSEUDOTYPE MONKEY IMMUNITY WEAK VIRUS VECTOR
JP2003503807A JPWO2002101057A1 (ja) 2001-06-08 2002-05-29 Vsv−gシュードタイプ化サル免疫不全ウイルスベクターを用いた霊長類胚性幹細胞への遺伝子導入
AU2002258248A AU2002258248C1 (en) 2001-06-08 2002-05-29 Gene transfer into primate embryonic stem cells using VSV-G pseudotyped simian immunodeficiency virus vector
KR1020037015930A KR100682708B1 (ko) 2001-06-08 2002-05-29 Vsv-g 슈도타입화 원숭이 면역부전 바이러스 벡터를 사용한 영장류 배아 줄기세포로의 유전자 도입

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Application Number Priority Date Filing Date Title
JP2001-174696 2001-06-08
JP2001174696 2001-06-08

Publications (2)

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WO2002101057A1 true WO2002101057A1 (fr) 2002-12-19
WO2002101057A8 WO2002101057A8 (fr) 2004-02-12

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Country Status (7)

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US (1) US7323337B2 (ja)
EP (1) EP1403374A4 (ja)
JP (2) JPWO2002101057A1 (ja)
KR (1) KR100682708B1 (ja)
CN (1) CN100557023C (ja)
CA (1) CA2449589A1 (ja)
WO (1) WO2002101057A1 (ja)

Cited By (40)

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WO2005087936A1 (ja) * 2004-03-16 2005-09-22 Dnavec Research Inc. 胚性幹細胞に遺伝子を導入する方法
WO2006090697A1 (ja) * 2005-02-23 2006-08-31 Dnavec Corporation Pedfおよびfgf2を含む眼組織細胞におけるアポトーシス変性を伴う疾患の治療薬
WO2007049749A1 (ja) 2005-10-28 2007-05-03 Dnavec Corporation 血液凝固異常の治療方法
US8278284B2 (en) 2005-02-23 2012-10-02 Masanori Miyazaki Therapeutic agents for diseases associated with apoptotic degeneration in ocular tissue cells that use SIV-PEDF vectors
WO2013168403A1 (ja) 2012-05-08 2013-11-14 株式会社大塚製薬工場 トレハロース含有肺塞栓形成予防用哺乳動物細胞懸濁液
WO2014208053A1 (ja) 2013-06-28 2014-12-31 株式会社大塚製薬工場 トレハロース及びデキストラン含有哺乳動物細胞移植用溶液
EP2993235A1 (en) 2005-10-28 2016-03-09 ID Pharma Co., Ltd. Gene transfer into airway epithelial stem cell by using simian lentiviral vector pseudotyped with sendai rna virus spike protein
WO2018005975A1 (en) 2016-07-01 2018-01-04 Research Development Foundation Elimination of proliferating cells from stem cell-derived grafts
WO2018084228A1 (ja) 2016-11-04 2018-05-11 国立大学法人東京大学 動物細胞又は動物組織の凍結保存用溶液、凍結物、及び凍結保存方法
WO2018097253A1 (ja) 2016-11-25 2018-05-31 国立研究開発法人理化学研究所 移植用細胞集団及びその製造方法
WO2018110159A1 (ja) 2016-12-14 2018-06-21 株式会社大塚製薬工場 哺乳動物細胞凍結保存液
WO2018164240A1 (ja) 2017-03-08 2018-09-13 大日本住友製薬株式会社 網膜色素上皮細胞の製造方法
WO2019017491A1 (ja) 2017-07-20 2019-01-24 国立研究開発法人理化学研究所 神経組織の保存方法
WO2019017492A1 (ja) 2017-07-20 2019-01-24 国立研究開発法人理化学研究所 連続的な上皮を含む網膜組織の成熟化方法
WO2019050015A1 (ja) 2017-09-08 2019-03-14 国立研究開発法人理化学研究所 網膜組織を含む細胞凝集体及びその製造方法
WO2019054514A1 (ja) 2017-09-14 2019-03-21 国立研究開発法人理化学研究所 網膜組織の製造方法
WO2019054515A1 (ja) 2017-09-14 2019-03-21 国立研究開発法人理化学研究所 背側化シグナル伝達物質又は腹側化シグナル伝達物質による錐体視細胞又は桿体視細胞の増加方法
WO2019103125A1 (ja) 2017-11-24 2019-05-31 住友化学株式会社 神経系細胞又は神経組織と非神経上皮組織とを含む細胞塊の製造方法及びその細胞塊
WO2019103129A1 (ja) 2017-11-24 2019-05-31 住友化学株式会社 下垂体組織を含む細胞塊の製造方法及びその細胞塊
WO2019160148A1 (ja) 2018-02-19 2019-08-22 大日本住友製薬株式会社 細胞凝集体、細胞凝集体の混合物及びそれらの製造方法
WO2020039732A1 (ja) 2018-08-24 2020-02-27 住友化学株式会社 嗅神経細胞又はその前駆細胞を含む細胞塊、及びその製造方法
WO2020066991A1 (ja) 2018-09-28 2020-04-02 株式会社大塚製薬工場 アカルボース又はスタキオースを含む哺乳動物細胞保存用液
WO2020100481A1 (ja) 2018-11-15 2020-05-22 Jsr株式会社 脳オルガノイドの製造方法
WO2020130068A1 (ja) 2018-12-20 2020-06-25 住友化学株式会社 胚型赤芽球を含む細胞集団及びその製造方法、細胞培養組成物並びに化合物試験法
WO2020138430A1 (ja) 2018-12-28 2020-07-02 国立研究開発法人理化学研究所 網膜系細胞又は網膜組織の障害を伴う疾患の治療薬
WO2020184720A1 (ja) 2019-03-13 2020-09-17 大日本住友製薬株式会社 移植用神経網膜の品質を評価する方法及び移植用神経網膜シート
WO2020218461A1 (ja) 2019-04-26 2020-10-29 株式会社大塚製薬工場 トレハロースを含む哺乳動物細胞保存用液
WO2020218480A1 (ja) 2019-04-26 2020-10-29 国立研究開発法人理化学研究所 神経網膜と網膜色素上皮細胞とハイドロゲルとを含む複合体及びその製造方法
WO2021045217A1 (ja) 2019-09-06 2021-03-11 学校法人慶應義塾 グリア前駆細胞を含む細胞凝集体の製造方法
WO2021100830A1 (ja) 2019-11-20 2021-05-27 大日本住友製薬株式会社 細胞凝集体の凍結方法
WO2021100829A1 (ja) 2019-11-20 2021-05-27 大日本住友製薬株式会社 神経系細胞の凍結方法
EP3868873A1 (en) 2014-10-24 2021-08-25 Sumitomo Dainippon Pharma Co., Ltd. Production method for retinal tissue
WO2022054925A1 (ja) 2020-09-11 2022-03-17 国立研究開発法人理化学研究所 神経網膜を含む細胞凝集体とマトリクスとを含む複合体及びその製造方法
WO2022054924A1 (ja) 2020-09-11 2022-03-17 大日本住友製薬株式会社 移植用組織のための媒体
WO2022079322A1 (en) 2020-10-16 2022-04-21 Fundació Centre De Regulació Genòmica Therapy for degenerative disease and tissue damage
WO2022191216A1 (ja) 2021-03-09 2022-09-15 国立研究開発法人理化学研究所 低免疫原性網膜色素上皮細胞の製造方法
EP4092109A1 (en) 2015-09-08 2022-11-23 Sumitomo Pharma Co., Ltd. Method for producing retinal pigment epithelial cells
WO2022265086A1 (ja) 2021-06-17 2022-12-22 国立大学法人京都大学 ヒト多能性幹細胞由来大脳皮質細胞製剤の製造方法
WO2023054396A1 (ja) 2021-09-30 2023-04-06 住友化学株式会社 下垂体組織を含む細胞集団の製造方法及びその細胞集団
WO2023054395A1 (ja) 2021-09-30 2023-04-06 住友ファーマ株式会社 下垂体ホルモン産生細胞を含む細胞凝集体及びその製造方法

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WO2007014282A2 (en) * 2005-07-26 2007-02-01 Whitehead Institute For Biomedical Research Transfection method and uses related thereto
MX342449B (es) 2007-08-03 2016-09-29 Pasteur Institut Vectores de transferencia de gen lentiviral y sus aplicaciones medicinales.
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EP1403374A1 (en) 2004-03-31
AU2002258248B2 (en) 2006-08-17
CN1571844A (zh) 2005-01-26
CN100557023C (zh) 2009-11-04
WO2002101057A8 (fr) 2004-02-12
EP1403374A4 (en) 2004-07-21
AU2002258248B8 (en) 2006-11-23
JPWO2002101057A1 (ja) 2004-09-24
CA2449589A1 (en) 2002-12-19
KR100682708B1 (ko) 2007-02-15
JP2008119006A (ja) 2008-05-29
KR20040003053A (ko) 2004-01-07
US7323337B2 (en) 2008-01-29

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