WO2002079251A3 - Method for making human insulin precursors - Google Patents

Method for making human insulin precursors Download PDF

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Publication number
WO2002079251A3
WO2002079251A3 PCT/DK2002/000191 DK0200191W WO02079251A3 WO 2002079251 A3 WO2002079251 A3 WO 2002079251A3 DK 0200191 W DK0200191 W DK 0200191W WO 02079251 A3 WO02079251 A3 WO 02079251A3
Authority
WO
WIPO (PCT)
Prior art keywords
human insulin
making human
insulin precursors
precursors
peptide
Prior art date
Application number
PCT/DK2002/000191
Other languages
French (fr)
Other versions
WO2002079251A2 (en
Inventor
Thomas Boerglum Kjeldsen
Svend Ludvigsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of WO2002079251A2 publication Critical patent/WO2002079251A2/en
Publication of WO2002079251A3 publication Critical patent/WO2002079251A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Novel insulin precursors comprising a connecting peptide (mini C-peptide) of up to 6 amino acid residues and comprising at least one Gly are provided. The precursors can be converted into human insulin or desB30 human insulin.
PCT/DK2002/000191 2001-04-02 2002-03-21 Method for making human insulin precursors WO2002079251A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200100548 2001-04-02
DKPA200100548 2001-04-02

Publications (2)

Publication Number Publication Date
WO2002079251A2 WO2002079251A2 (en) 2002-10-10
WO2002079251A3 true WO2002079251A3 (en) 2003-04-10

Family

ID=8160416

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2002/000191 WO2002079251A2 (en) 2001-04-02 2002-03-21 Method for making human insulin precursors

Country Status (1)

Country Link
WO (1) WO2002079251A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1066328B1 (en) * 1998-03-31 2008-09-17 Tonghua Gantech Biotechnology Ltd. Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
US20160115216A1 (en) * 2013-06-07 2016-04-28 Novo Nordisk A/S Method for Making Mature Insulin Polypeptides
US11230585B2 (en) 2016-09-06 2022-01-25 Chemical & Biopharmaceutical Laboratories Of Patra Proinsulin derivatives
CN106749616B (en) * 2016-12-19 2021-09-03 华润昂德生物药业有限公司 Preparation method of human insulin crystal with threonine B30 deleted

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0055945A2 (en) * 1981-01-02 1982-07-14 Genentech, Inc. Human proinsulin and analogs thereof and method of preparation by microbial polypeptide expression and conversion thereof to human insulin
US5324641A (en) * 1988-06-20 1994-06-28 Novo Nordisk A/S DNA sequences encoding insulin precursors and methods of production
US5840542A (en) * 1995-07-28 1998-11-24 Mogam Biotechnology Research Institute Method for manufacture of proinsulin with high export yield
US5962267A (en) * 1995-02-15 1999-10-05 Hanil Synthetic Fiber Co., Ltd. Proinsulin derivative and process for producing human insulin
WO2001049870A1 (en) * 1999-12-29 2001-07-12 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0055945A2 (en) * 1981-01-02 1982-07-14 Genentech, Inc. Human proinsulin and analogs thereof and method of preparation by microbial polypeptide expression and conversion thereof to human insulin
US5324641A (en) * 1988-06-20 1994-06-28 Novo Nordisk A/S DNA sequences encoding insulin precursors and methods of production
US5962267A (en) * 1995-02-15 1999-10-05 Hanil Synthetic Fiber Co., Ltd. Proinsulin derivative and process for producing human insulin
US5840542A (en) * 1995-07-28 1998-11-24 Mogam Biotechnology Research Institute Method for manufacture of proinsulin with high export yield
WO2001049870A1 (en) * 1999-12-29 2001-07-12 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 54, no. 3, September 2000 (2000-09-01), pages 277 - 286, ISSN: 0175-7598 *
BEIJING DAXUE XUEBAO, ZIRAN KEXUEBAN, vol. 30, no. 6, 1994, pages 722 - 727, ISSN: 0479-8023 *
CHANG S-G ET AL: "HUMAN INSULIN PRODUCTION FROM A NOVEL MINI-PROINSULIN WHICH HAS HIGH RECEPTOR-BINDING ACTIVITY", BIOCHEMICAL JOURNAL, PORTLAND PRESS, LONDON, GB, vol. 329, 1998, pages 631 - 635, XP001037629, ISSN: 0264-6021 *
CHUL SOO SHIN ET AL: "Enhanced production of human mini-proinsulin in fed-batch cultures at high cell density of Escherichia coli BL21(DE3)[pET-3aT2M2].", BIOTECHNOL. PROG., vol. 13, 1997, pages 249 - 257, XP002902646 *
DATABASE DIALOG, MEDLINE FILE 155 [online] KJELDSEN T.: "Yeast secretory expression of insulin precursors.", XP002902647, retrieved from DIALOG accession no. 10931544 Database accession no. 20483479 *
DATABASE DIALOG,MEDLINE FILE 155 [online] WETZEL R ET AL: "Expression in escherichia coli of a chemically synthesized gene for a "mini-C" analog of human proinsulin.", XP002902643, retrieved from DIALOG accession no. 03926478 Database accession no. 82211803 *
DATABASE STN INTERNATIONAL [online] FILE CAPLUS; WEI GE ET AL: "Construction and expression of the gene for "mini-C" analog of human proinsulin.", XP002902644, retrieved from STN accession no. 1995:419763 Database accession no. 123:2185 *
ERIKA E. BÜLLESBACH: "Semisynthesis of linear proinsulin analogues with shortened C-peptides.", CHEMISTRY OF PEPTIDES AND PROTEINS, vol. 2, 1984, pages 23 - 28, XP002902645 *
GENE, vol. 16, no. (1-3), December 1981 (1981-12-01), pages 63 - 71, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
WO2002079251A2 (en) 2002-10-10

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