WO2002078834A3 - Procede et dispositif reposant sur l'emploi de capillaires en faisceau pour criblage a fort debit - Google Patents

Procede et dispositif reposant sur l'emploi de capillaires en faisceau pour criblage a fort debit Download PDF

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Publication number
WO2002078834A3
WO2002078834A3 PCT/US2002/005316 US0205316W WO02078834A3 WO 2002078834 A3 WO2002078834 A3 WO 2002078834A3 US 0205316 W US0205316 W US 0205316W WO 02078834 A3 WO02078834 A3 WO 02078834A3
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Prior art keywords
capillaries
methods
reaction
disclosed
capillary
Prior art date
Application number
PCT/US2002/005316
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English (en)
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WO2002078834A2 (fr
Inventor
Jianming Xiao
Shiping Chen
Yuling Luo
John Butler
Masoud Qazen
Jochen Thiele
Anthony Chen
Original Assignee
Genospectra Inc
Jianming Xiao
Shiping Chen
Yuling Luo
John Butler
Masoud Qazen
Jochen Thiele
Anthony Chen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/791,411 external-priority patent/US20010055801A1/en
Priority claimed from US09/791,410 external-priority patent/US20020028160A1/en
Application filed by Genospectra Inc, Jianming Xiao, Shiping Chen, Yuling Luo, John Butler, Masoud Qazen, Jochen Thiele, Anthony Chen filed Critical Genospectra Inc
Priority to AU2002255581A priority Critical patent/AU2002255581A1/en
Publication of WO2002078834A2 publication Critical patent/WO2002078834A2/fr
Publication of WO2002078834A3 publication Critical patent/WO2002078834A3/fr

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract

Cette invention concerne des systèmes et des procédés utilisés pour le criblage à fort débit de composés présentant une affinité pour une cible biologique. Le système repose sur l'emploi d'un faisceau de capillaires présentant deux extrémités distinctes, les capillaires étant reliés à une extrémité à des composés stockés dans des réservoirs séparés et étant reliés et traités à l'autre extrémité de manière à former un microréseau bidimensionnel. L'invention porte sur un faisceau de capillaires qui comportent à l'une de leurs extrémités des puits de réaction pour hybridation et réaction des composés. Les capillaires peuvent être photoconducteurs. L'invention concerne également divers procédés permettant d'identifier un composant cible dans un liquide au moyen dudit faisceau de capillaires, ainsi que des procédés de fabrication de ce faisceau. Elle porte également sur une chambre de réaction guidée par effet tensioactif, ainsi que sur des procédés et des opérations chimiques permettant de fabriquer et d'utiliser une telle chambre de réaction pour des essais de liaison et d'hybridation. Sont en outre décrits des systèmes garantissant un dosage précis de liquides dans les capillaires et au niveau des chambres de réaction, y compris du puits de réaction « virtuel » guidé par effet tensioactif. L'invention concerne également des criblages à fort débit réalisés au moyen de capillaires recouverts intérieurement de fibre optique. Les ensembles de capillaires s'utilisent aussi bien avec des sondes liquides qu'avec des sondes immobilisés sur leurs parois.
PCT/US2002/005316 2001-02-16 2002-02-19 Procede et dispositif reposant sur l'emploi de capillaires en faisceau pour criblage a fort debit WO2002078834A2 (fr)

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AU2002255581A AU2002255581A1 (en) 2001-02-16 2002-02-19 Bundled capillaries apparatus for high throughput screening

Applications Claiming Priority (18)

Application Number Priority Date Filing Date Title
US26964201P 2001-02-16 2001-02-16
US60/269,642 2001-02-16
US27100201P 2001-02-22 2001-02-22
US60/271,002 2001-02-22
US09/791,411 US20010055801A1 (en) 2000-02-22 2001-02-22 Liquid arrays
US09/791,410 US20020028160A1 (en) 2000-02-22 2001-02-22 Method and apparatus based on bundled capillaries for high throughput screening
US09/791,410 2001-02-22
US09/791,411 2001-02-22
US30273501P 2001-07-03 2001-07-03
US60/302,735 2001-07-03
US31474701P 2001-08-24 2001-08-24
US60/314,747 2001-08-24
US31528501P 2001-08-27 2001-08-27
US60/315,285 2001-08-27
US32768601P 2001-10-04 2001-10-04
US60/327,686 2001-10-04
US35727502P 2002-02-15 2002-02-15
US60/357,275 2002-02-15

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