WO2002072031A2 - Substituted tetracycline compounds as synergistic antifungal agents - Google Patents
Substituted tetracycline compounds as synergistic antifungal agents Download PDFInfo
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- WO2002072031A2 WO2002072031A2 PCT/US2002/007829 US0207829W WO02072031A2 WO 2002072031 A2 WO2002072031 A2 WO 2002072031A2 US 0207829 W US0207829 W US 0207829W WO 02072031 A2 WO02072031 A2 WO 02072031A2
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- 0 Cc1ccc(CCc(cc2)c(CC(CC([C@](*)C(O)=C3C(*)=O)[C@@]4(*)C3=O)C(C3=O)=C4O)c3c2O)cc1 Chemical compound Cc1ccc(CCc(cc2)c(CC(CC([C@](*)C(O)=C3C(*)=O)[C@@]4(*)C3=O)C(C3=O)=C4O)c3c2O)cc1 0.000 description 1
- HXITXNWTGFUOAU-UHFFFAOYSA-N OB(c1ccccc1)O Chemical compound OB(c1ccccc1)O HXITXNWTGFUOAU-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
- A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/65—Tetracyclines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
Definitions
- mycoses fungal diseases
- mycotic infections are superficial in nature, are not life threatening, and provide little medical impetus to pharmaceutical companies to develop novel treatments. This scenario is changing, however, and while death from fungal disease is not new, the incidence of systemic fungal infections that cause these fatalities is increasing.
- AIDS Acquired Immuno Deficiency Syndrome
- Fungal disorders can, thus, be divided into the life-threatening systemic infections, such as histoplasmosis, systemic candidiasis, aspergiUosis, blastomycosis, coccidioidomycosis, paracoccidioidomycosis, and cryptococcosis, and the more common superficial ones, such as dermatophyte (ringworm) infections, for example, tinea pedis (athlete's foot) and tinea cruris (jock itch), candidiasis, and actinomycosis.
- ringworm dermatophyte
- the life-threatening fungal infections are a growing problem not only for immunosuppressed or immunocompromised individuals as noted above but individuals with other viral infections, such as cytomegalovirus (CMN), and influenza, for cancer patients receiving chemotherapy or radiotherapy, for transplant patients receiving antirejection agents, and for patients that have received toxic chemicals, metals and radiation exposure.
- CNN cytomegalovirus
- influenza cytomegalovirus
- cancer patients receiving chemotherapy or radiotherapy for transplant patients receiving antirejection agents, and for patients that have received toxic chemicals, metals and radiation exposure.
- Mycoses are often caused by fungi which are opportunists, rather than pathogens.
- Candidiasis, aspergiUosis, phycomycosis, nocardiosis, and cryptococcosis are typically opportunistic fungal infections.
- Candida albicans is normally found in the alimentary tract as a commensal, yet it is a major cause of systemic fungal infections in immunocomprised patients and topical infections in healthy individuals.
- fungistatic activity is the ability to prevent growth of fungi
- fungicidal activity is the ability to kill the fungi.
- Many agents used in the treatment of superficial mycoses are virtually devoid of either fungistatic or fungicidal actions in the concentrations used, and their beneficial effects probably depend upon factors not related to any direct effect on fungi.
- the invention pertains, at least in part, to methods for increasing the antifungal activity of an antifungal agent.
- the method includes administering the antifungal agent with an effective amount of a substituted tetracycline compound, such that the antifungal activity of the antifungal agent is increased.
- antifungal agents include polyenes such as amphotericin B.
- substituted tetracycline compounds include compounds of formula I:
- R , R , R , and R are each independently hydrogen, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, arylalkyl, aryl, heterocyclic, heteroaromatic or a prodrug moiety;
- R 4 is NR 4 R 4 , alkyl, alkenyl, alkynyl, hydroxyl, halogen, or hydrogen;
- R , R 10 , R ! and R 12 are each hydrogen or a pro-drug moiety
- Z' is O, S, or NR 9f ;
- W is CR 7d R 7e , S, NR 7b or O;
- W' is O, NR 7f S;
- R 7a , R 7b , R 7c , R 7d , R 7e , R 9a , R 9b , R 9c , R 9d , and R 9e are each independently hydrogen, acyl, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, arylalkyl, aryl, heterocyclic, heteroaromatic or a prodrug moiety;
- R is hydrogen, hydroxyl, halogen, thiol, alkyl, alkenyl, alkynyl, aryl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, or an arylalkyl;
- the invention pertains, at least in part, to methods for increasing the antifungal activity of an antifungal agent, by administering an antifungal agent in combination with an effective amount of a substituted tetracycline compound.
- unsubstituted minocycline and doxycycline have been shown to possess limited antifungal activity both alone and in synergy with amphotericin B (Antimicrob. Agents Chemother. (1984), 26(6)837-40; Pathol Biol. (1975) 23(9):725-8).
- both unsubstituted minocycline and doxycycline are limited by both low synergistic activity as well as high levels of cytotoxicity.
- the fungi of the invention includes fungi of the genus Candida (e.g., C. tropicalis, C. parapsilosis, C. lusitaniae, C krusei, C guilliermondii, C. glabrata, C dubliniensis, and C. albicans).
- Candida e.g., C. tropicalis, C. parapsilosis, C. lusitaniae, C krusei, C guilliermondii, C. glabrata, C dubliniensis, and C. albicans.
- antifungal agent includes agents which are known in the art to have fungistatic or fungicidal activity, which can be synergistically increased using the compounds of the invention.
- antifungal agents include but are not limited to, azoles (e.g., Fluconazole®, Itraconazole®, Ketoconazole®, Miconazole®, Clortrimazole®, Noriconazole ®, Posaconazole®, Rovuconazole®, etc.), polyenes (e.g., natamycin, lucensomycin, nystatin, amphotericin B, etc.), echinocandins (e.g., Cancidas®), pradimicins (e.g., beanomicins, nikkomycins, sordarins, allylamines, etc.) and derivatives and analogs thereof.
- azoles e.g., Fluconazole®, Itraconazole®,
- antifungal activity includes inhibiting the growth of a fungus (e.g., fungistatic activity), killing at least a portion of the fungus (e.g., fungicidal activity), limiting the ability of the fungus to reproduce, etc.
- inhibiting the growth of a fungus includes both fungistatic and fungicidal activity.
- Fungistatic activity includes any decrease in the rate of growth of a fungal colony. Fungistatic activity may be manifested by a fungus maintaining its present size or failing to colonize the surrounding areas. Fungistatic activity may be a result of inhibition of the fungal reproductive processes.
- Fungicidal activity generally includes, for example, irraditication of a fungus or fungal colony, killing a fungus or fungal colony or, in one embodiment, a decrease in the mass or size of a fungus or fungal colony.
- the antifungal activity of the antifungal agent is increased when administered in combination with a substituted tetracycline compound of the invention, thereby reducing the effective amount of the antifungal agent required as compared to the amount required when the antifungal agent is administered alone.
- the coadministration of a substituted tetracycline compound of the invention reduces the effective amount of the antifungal agent by 1 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold, as compared to the effective amount of the antifungal agent alone, e.g., without the aid of a substituted tetracycline compound or another synergistic agent.
- the substituted tetracycline compound has low cell toxicity and may exhibit low (or, in some embodiments, no) antibacterial activity, e.g., as measured in Example 4.
- Substituted tetracycline compounds with low antibacterial activity may be compounds with MIC of 4 ⁇ m or greater.
- the substituted tetracycline compounds of the invention may have anti-inflammatory activity, e.g., as measured by art recognized assays.
- the cell toxicity of particular substituted tetracycline compounds, antifungal agents, and combinations thereof can be measured using the assay given in Example 3.
- the language "effective amount " of the antifungal agent is the amount necessary or sufficient to inhibit the growth of fungus, or in certain instances, to kill the fungus. In an embodiment, the effective amount of the antifungal agent is reduced when administered in combination with a substituted tetracycline compound of the invention.
- tetracycline compounds includes tetracycline family members such as methacycline, sancycline, apicycline, clomocycline, guamecycline, meglucycline, mepylcycline, penimepicycline, pipacycline, etamocycline, penimocycline, etc. as well as other tetracycline compounds having the characteristic naphthacene A-B-C-D ring structure. Additional tetracycline compounds can be found, for example, in U.S. Patent Application Serial No.: 09/234,847, and U.S. Patents Nos.
- substituted tetracycline compounds includes tetracycline compounds which have at least one substitution, e.g., at the 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 1 la, 12, 12a, or, for methacycline, the 13 position, which allows the compound to perform its intended function, e.g., synergistically inhibit the growth of fungus.
- substituted tetracycline compounds does not include unsubstituted tetracycline, minocycline, or doxycycline.
- the substituted tetracycline compounds of the invention reduce the MIC of amphotericin B to a larger extent than unsubstituted doxycycline, tetracycline, or minocycline.
- substituted tetracycline compound includes, for example, substituted sancycline compounds, substituted minocycline compounds and substituted doxycycline compounds.
- the FIC of a substituted tetracycline compound of the invention is 0.125 or less, 0.09 or less, 0.08 or less, 0.07 or less, 0.063 or less, etc. Values and ranges included and/or intermediate within the ranges set forth herein are also intended to be within the scope of the present invention.
- R 2 , R 2 , R 4 , and R 4 are each independently hydrogen, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, arylalkyl, aryl, heterocyclic, heteroaromatic or a prodrug moiety;
- R 4 is NR 4 R 4 , alkyl, alkenyl, alkynyl, hydroxyl, halogen, or hydrogen;
- R , R , R and R are each hydrogen or a pro-drug moiety
- R 5 is hydroxyl, hydrogen, thiol, alkanoyl, aroyl, alkaroyl, aryl, heteroaromatic, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, arylalkyl, alkyl carbonyloxy, or aryl carbonyloxy; R and R are each independently hydrogen, methylene, absent, hydroxyl, halogen, thiol, alkyl, alkenyl, alkynyl, aryl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, or an arylalkyl;
- Z' is O, S, or NR 9f ;
- W is CR 7d R 7e , S, NR 7b or O;
- W' is O, NR 7f S;
- R 7a , R 7b , R 7c , R 7d , R 7e , R 9a , R 9b , R 9c , R 9d , and R 9e are each independently hydrogen, acyl, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, arylalkyl, aryl, heterocyclic, heteroaromatic or a prodrug moiety;
- R 8 is hydrogen, hydroxyl, halogen, thiol, alkyl, alkenyl, alkynyl, aryl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, or an arylalkyl;
- R is hydrogen, hydroxy, alkyl, alkenyl, alkynyl, aryl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, or an arylalkyl;
- Y' and Y are each independently hydrogen, halogen, hydroxyl, cyano, sulfhydryl, amino, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, or an arylalkyl, and pharmaceutically acceptable salts thereof.
- substituted tetracycline compounds used in the methods and compositions of the invention are substituted sancycline compounds, e.g., with substitution at the, for example, 2, 5, 6, 7,8, 9, 10, 11, 1 la, 12 12a position and/or, in the case of minocycline,
- R , R , R , R , and R are each hydrogen or a prodrug moiety; R 4 and R 4 are each alkyl (e.g., lower alkyl, e.g., methyl); X is CR R ; and R , R , R , R , and R are each, generally, hydrogen.
- the substituted tetracycline compound is a substituted tetracycline (e.g., generally, wherein R 4 is NR 4 R 4" , R 4' and R 4" are methyl, R 5 is hydrogen and X is CR 6 R 6' , wherein R 6 is methyl and R 6 is hydroxy); substituted doxycycline (e.g., wherein R 4 is NR 4 R 4 , R 4 and R 4 are methyl, R 5 is hydroxyl and X is CR 6 R 6 , wherein R 6 is methyl and R 6 is hydrogen); substituted minocycline (e.g., wherein R 4 is NR 4 R 4 , R 4 and R 4 are methyl; R 5 is hydrogen and X is CR 6 R 6 wherein R 6 and R 6 are hydrogen atoms and R 7 is dimethylamino) or substituted sancycline (wherein R 4 is NR 4 R 4 , R 4 and R 4" are methyl;
- R 5 is substituted, e.g., not hydrogen or hydroxy.
- R 5 is an ester (alkcarbonyloxy).
- R 5 is an alkyl ester.
- examples of R 5 include alkyl esters such as C ⁇ -C ⁇ 2 alkyl, alkenyl, alkynyl, or aryl esters.
- the alkyl groups may be straight chains, branched chains, and/or contain rings.
- esters include, but are not limited to, tetracycline esters of ethanoic acid, propanoic acid, pentanoic acid, hexanoic acid, 2-cyclopentane ethanoic acid, cyclopentanoic acid, cycloheptanoic acid, 2- methyl propanoic acid, cyclohexanoic acid, and adamantane 2-carboxylic acid.
- R 5 is hydrogen.
- the substituted tetracycline compounds used in the methods and compositions of the invention are substituted sancycline compounds, e.g., with substitution at the, for example, 2, 7 and/or 9 position.
- R 2 , R 3 , R 10 , R 11 , and R 12 are each hydrogen or a prodrug moiety;
- R 4 is dialkylamino and R 4 is hydrogen;
- X is CR 6 R 6 ; and
- R 2 , R 5 , R 6 , R 6 , and R 8 are each, generally, hydrogen.
- R 9 may be hydrogen.
- R 4 is hydrogen.
- R 7 is substituted or unsubstituted aryl, e.g., heteroaryl, phenyl, etc.
- R 7 substituents include substituents which allow the substituted tetracycline compound to perform its intended function.
- substituents include, but are not limited to, alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate, aralkyl, phosphonato, phosphinato, cyano, amino, acylamino, amido, imino, sulfhydryl, alkylthio,
- R 7 is substituted or unsubstituted heteroaryl.
- heteroaromatic groups include both monocyclic and polycyclic (e.g., multicylic rings), such as, but not limited to, furanyl, imidazolyl, benzothiophenyl, benzofuranyl, quinolinyl, isoquinolinyl, pyridinyl, pyrazolyl, benzodioxazolyl, benzoxazolyl, benzothiazolyl, benzoimidazolyl, methylenedioxyphenyl, indolyl, thienyl, pyrimidyl, pyrazinyl, purinyl, pyrazolyl, oxazolyl, isooxazolyl, naphthridinyl, thiazolyl, isothiazolyl, and deazapurinyl.
- R is benzofuranyl.
- substituents include all substituents which allow the tetracycline compound to perform its intended function, such as but are not limited to, alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate, aralkyl, phosphonato, phosphinato, cyan
- R is substituted or unsubstituted, branched, straight chain or cyclic alkyl.
- substituents include substituents which allow the substituted tetracycline compound to perform its intended function, such as, but not limited to, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, trialkylsilyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate
- the substituents include heterocycles, substituted and unsubstituted phenyl, a hydroxy, and combinations thereof.
- the substituents of the alkyl R 7 may also be further substituted, if chemically possible, with the substituents for R 7 groups listed above.
- alkyl R 7 groups include Ci-C ⁇ groups, -Cio groups, Ci-C groups, etc., such as, but not limited to, 2-ethyl pentyl, methyl, ethyl, propyl, pentyl, hexyl, heptyl, etc. Values and ranges included and/or intermediate within the ranges set forth herein are also intended to be within the scope of the present invention.
- a C ⁇ C ? group includes groups with 1, 2, 3, 4, 5, 6, and 7 carbons.
- R 7 is substituted or unsubstituted alkenyl.
- substituents include substituents which allow the substituted tetracycline compound to perform its intended function.
- substituents include, but are not limited to, alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate, aralkyl
- an alkenyl R 7 moiety is substituted with a substituted or unsubstituted cyclic moiety.
- Cyclic moieties include both carbocyclic, heterocyclic, aryl, heteroaryl, cycloalkenyl, and cycloalkyl groups. Examples of cyclic moieties include, for example, cyclobutane, cylopentane, cyclohexane, phenyl, etc.
- the cyclic moiety can be substituted, e.g., with any substituent listed above for alkenyl R moieties.
- R 7 may also be linked to another tetracycline ring structure through a linking moiety.
- the linking moiety can be any length which allows the substituted tetracycline compound to perform its intended function.
- the linking moiety can be attached to the second tetracycline ring structure at any position that allows for such a substitution.
- the linker is alkyl, alkenyl, or alkynyl.
- the linker may be from about C 1 -C 25 , C ⁇ -C 20 , -C ⁇ , etc.
- the linker is alkynyl and the second tetracycline ring structure is sancyclyl.
- tetracycline dimer refers to compounds wherein two tetracycline ring structures are connected through chemical, e.g., covalent bonds, e.g., a linking moiety.
- R 7 is substituted or unsubstituted alkynyl.
- substituents include substituents which allow the substituted tetracycline compound to perform its intended function, such as but are not limited to, for alkynyl R 7 moieties include alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate, aralkyl, phosphonato, phosphinato, phosphat
- the R 7 alkynyl moiety may be substituted with a substituted or unsubstituted cyclic moiety.
- Cyclic moieties include both carbocyclic, heterocyclic, aryl, heteroaryl, cycloalkenyl, and cycloalkyl groups. Examples of cyclic moieties include, for example, cycloalkyls such as cyclobutane, cylopentane, cyclohexane, etc.
- the cyclic moiety can be substituted, e.g., with any substituent listed above for alkynyl R 7 moieties.
- R 7 moieties examples include, but are not limited to, phenyl, cyclohexyl, p-nitro phenyl, p- methyl phenyl, cyclohexene, and 1 -hydroxy cyclohexane.
- R 7 groups include substituted and unsubstituted alkyl carbonyl groups. These groups can be further substituted with aryl, alkyl, arylamino, alkenyl, alkoxy, or other substituents which allow the substituted tetracycline compound to perform its intended function.
- Another example of an R 7 moiety includes substituted and unsubstituted amino.
- the amino group can be dialkylamino, alkylamino, alkenylamino, arylamino, arylalkylamino, etc. or any other combination of substituents which allow it to perform its intended function, e.g., reduce the effective amount of a antifungal agent.
- the invention also pertains to methods and pharmaceutical compositions comprising 7,9- disubstituted tetracycline compounds, e.g., tetracycline compounds wherein the 7 and 9 position are substituted.
- the invention pertains to 7, 9-substituted sancycline compounds, e.g., compounds wherein X is CR R ; and R , R , R , R , and R are each hydrogen.
- the invention includes compounds wherein R 9 is alkyl and R 7 is substituted or unsubstituted aminomethyl.
- the invention includes compounds with any combination of substituents as described above for R 7 combined with any possible other substituent at another position, e.g., R 9 .
- the invention pertains to substituted doxycycline compounds wherein R 5 is hydroxy or alkylcarbonyloxy; X is CHR 6 ; R 6 is alkyl (e.g., lower alkyl, e.g.,
- substituents include alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate, aralkyl, phosphonato, phosphinato, cyano, amino, acylamino, amido, imino, sulfhydryl, alkylthio, sulfate, ary
- R 9 examples include substituted or unsubstituted alkyl (e.g., methyl, ethyl, propyl, t-butyl, n-butyl, i-butyl, pentyl, etc.), aryl, or any other substituent which allows the compound to perform its intended function.
- alkyl e.g., methyl, ethyl, propyl, t-butyl, n-butyl, i-butyl, pentyl, etc.
- aryl or any other substituent which allows the compound to perform its intended function.
- the invention pertains to methods and compositions which the substituted tetracycline compound is a substituted minocycline compound.
- these compounds include compounds wherein X is CR 6 R 6' ; R 2 , R 5 , R 6 , R 6 , and R 8 are each hydrogen, and R is dialkyl amino, e.g., dimethyl amino.
- the substituent may comprise one or more nitrogen atoms.
- R 9 is substituted or unsubstituted aryl (e.g., phenyl biaryl, heteroaryl, etc.) or araalkyl. R 9 may besubstituted or unsubstituted heteroaryl.
- heteroaromatic groups include both monocyclic and polycyclic (e.g., multicylic rings), such as, but not limited to, furanyl, imidazolyl, benzothiophenyl, benzofuranyl, quinolinyl, isoquinolinyl, pyridinyl, pyrazolyl, benzodioxazolyl, benzoxazolyl, benzothiazolyl, benzoimidazolyl, methylenedioxyphenyl, indolyl, thienyl, pyrimidyl, pyrazinyl, purinyl, pyrazolyl, oxazolyl, isooxazolyl, naphthridinyl, thiazolyl, isothiazolyl, and deazapurinyl.
- monocyclic and polycyclic e.g., multicylic rings
- R 7 is benzofuranyl.
- substituents include all substituents which allow the tetracycline compound to perform its intended function, such as but are not limited to, alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate, aralkyl, phosphonato, phosphinato,
- R 7 is thienyl.
- R 7 may also be substituted or unsubstituted heterocyclic, e.g., morpholinyl, piperazinyl, piperidinyl, etc.
- the aryl R 9 moiety is substituted or unsubstituted phenyl.
- R 9 moieties include substituted and unsubstituted, cyclic, branched or straight chain alkyl (e.g., -C1 5 , .Cio, e.g., methyl, ethyl, propyl, butyl, pentyl, hexyl, 2- cyclopentane ethyl, etc.).
- alkyl e.g., -C1 5 , .Cio, e.g., methyl, ethyl, propyl, butyl, pentyl, hexyl, 2- cyclopentane ethyl, etc.
- substituents include, but are not limited to, alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate, aralkyl, phosphonato, phosphinato, cyano, amino, acylamino, amido, imino, sulfhydryl, alkylthio, s
- substituents include those listed above substituents selected from the group consisting of amido, alkyl, aminoalkyl, heterocycle, carboxylic acid, formyl, chlorine, fluorine, or acetyl.
- R 9 include both substituted and unsubstituted or unsubstituted alkenyl or alkynyl.
- substituents include, but are not limited to, alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate, aralkyl, phosphonato, phosphinato, cyano, amino, acylamino, amido, imino, sulfhydryl, alkylthio, s
- the alkynyl R 9 moiety is substituted with one or more substituents selected from the group consisting of substituted and unsubstituted aryl, substituted and unsubstituted alkyl, carboxylic acid, cycloalkyl, cycloalkenyl, or alkoxycarbonyl.
- R 9 is substituted or unsubstituted alkyl or alkylamino.
- R 9 may be C ⁇ Cis alkyl, Cj- o alkyl, etc.
- R 9 may be substituted with groups such as aminoalkyl, hydroxy, halogens and other substituents which allow the substituted tetracycline compounds to perform their intended function.
- R a comprises substituted or unsubstituted phenyl.
- substituents for the substituted phenyl include, for example, alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate, aralkyl, phosphonato, phosphinato, cyano, amino, acylamino
- R may be heterocyclic, e.g., morpholinyl, pyridinyl, pyrazinyl, piperdinyl, etc.
- substituents may further be substituted with substituents such as, but not limited to, alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate,
- R 7 and R 9 may also be hydrogen or another moiety which allows for the substituted tetracycline compounds of the invention to perform their intended function.
- R 13 is substituted or unsubstituted aryl, e.g., phenyl, biaryl, heteroaryl, etc. Examples of substituents include those which allow the substituted tetracycline compound to perform its intended function.
- Examples include but are not limited to, alkyl, alkenyl, alkynyl, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, alkyloxycarbonyl, carboxy, arylcarbonyloxy, alkoxycarbonylamino, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aminoalkyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, silyl, aminocarbonyl, alkylthiocarbonyl, phosphate, aralkyl, phosphonato, phosphinato, cyano, amino, acylamino, amido, imino, sulfhydryl, alkylthio, sulfate,
- the substituted tetracycline compounds of Formula (I) include compounds with large hydrophobic moieties at the 7, 9 or 13 position.
- the hydrophobic moieties may be partially sterically rigid (e.g., contain double or triple bonds, or contain one or more rings).
- the compounds may comprise a substituted or unsubstituted aryl (e.g., heteroaryl, phenyl, etc. ring) group or one or more alkyl groups.
- the compounds may comprise a group with one or more nitrogen or other other heteroatoms.
- the compound may be a 9-substituted minocycline compound.
- the substituted tetracycline compounds may comprise any combination of substituents shown in Table 2.
- the invention also pertains to 4-dedimethylaminotetracycline compounds with the substituents described herein or shown in Table 2 (e.g., compounds with the same substituents as described herein or in Table 2, except at the R 4 position where the shown dimethylamino group is a hydrogen.)
- Examples of substituted tetracycline compounds which can be used in the methods of the invention are shown below and in Table 2.
- the substituted tetracycline compounds of the invention can be synthesized using the methods described in Example 1, the following schemes, and art recognized techniques. All novel substituted tetracycline compounds described herein are included in the invention as compounds.
- the 7- or 9-amino tetracycline compound (IE and IF, respectively) is treated with HONO, to yield the diazonium salt (1G and IH).
- the salt (1G and IH) is treated with an appropriate halogenated reagent (e.g., R 9 Br, wherein R 9 is an aryl, alkenyl, or alkynyl moiety) to yield the desired compound(e.g., in Scheme 1, 7-cyclopent-l-enyl doxycycline (IH) and 9-cyclopent-l-enyl doxycycline (II)).
- tetracycline compounds of the invention wherein R 7 is a carbamate or a urea derivative can be synthesized using the following protocol.
- Sancycline (2A) is treated with NaNO 2 under acidic conditions forming 7-nitro sancycline (2B) in a mixture of positional isomers.
- 7-nitrosancycline (2B) is then treated with Hi gas and a platinum catalyst to form the 7-amino sancycline derivative (2C).
- isocyanate (2D) is reacted with the 7-amino sancycline derivative (2C).
- carbamate (2G) the appropriate acid chloride ester (2F) is reacted with 2C.
- tetracycline compounds of the invention wherein R 7 is a heterocyclic (i.e. thiazole) substituted amino group can be synthesized using the above protocol.
- 7-amino sancycline (3 A) is reacted with Fmoc-isothiocyanate (3B) to produce the protected thiourea (3C).
- the protected thiourea (3C) is then deprotected yielding the active sancycline thiourea (3D) compound.
- the sancycline thiourea (3D) is reacted with an ⁇ - haloketone (3E) to produce a thiazole substituted 7-amino sancycline (3F).
- 7- alkenyl tetracycline compounds such as 7-alkynyl sancycline (4A) and 7-alkenyl sancycline (4B), can be hydrogenated to form alkyl 7- substituted tetracycline compounds (e.g. 7-alkyl sancycline, 4C).
- Scheme 4 depicts the selective hydrogenation of the 7- position double or triple bond, in saturated methanol and hydrochloric acid solution with a palladium/carbon catalyst under pressure, to yield the product.
- a general synthetic scheme for synthesizing 7-position aryl derivatives is shown.
- a Suzuki coupling of an aryl boronic acid with an iodosancycline compound is shown.
- An iodo sancycline compound (5B) can be synthesized from sancycline by treating sancycline (5 A) with at least one equivalent N-iodosuccinimide (NIS) under acidic conditions. The reaction is quenched, and the resulting 7-iodo sancycline (5B) can then be purified using standard techniques known in the art.
- NIS N-iodosuccinimide
- 7-iodo sancycline (5B) is treated with an aqueous base (e.g., Na 2 CO 3 ) and an appropriate boronic acid (5C) and under an inert atmosphere.
- the reaction is catalyzed with a palladium catalyst (e.g., Pd(OAc) 2 ).
- the product (5D) can be purified by methods known in the art (such as HPLC).
- Other 7-aryl and alkynyl tetracycline compounds can be synthesized using similar protocols.
- 7- and 9- carbonylated compounds can be synthesized using art recognized techniques.
- the compounds of the invention can also be synthesized using Heck-type cross coupling reactions.
- Heck-type cross-couplings can be performed by suspending a halogenated tetracycline compound (e.g., 6-iodosancycline, 6A) and an appropriate palladium or other transition metal catalyst (e.g., Pd(OAc) 2 and Cul) in an appropriate solvent (e.g., degassed acetonitrile).
- a reactive alkene (6B) or alkyne (6D), and triethylamine are then added and the mixture is heated for several hours, before being cooled to room temperature.
- the resulting 7-substituted alkenyl (6C) or 7-substituted alkynyl (6E) tetracycline compound can then be purified using techniques known in the art.
- 5-esters of 9- substituted tetracycline compounds can be formed by dissolving the 9- substituted compounds (8A) in strong acid (e.g. HF, methanesulphonic acid, and trifluoromethanesulfonic acid) and adding the appropriate carboxylic acid to yield the corresponding esters (8B).
- strong acid e.g. HF, methanesulphonic acid, and trifluoromethanesulfonic acid
- methacycline (9 A) can be reacted with a phenylboronic acid in the presence of a palladium catalyst such as Pd(OAc) to form a 13 aryl substituted methacycline compound.
- a palladium catalyst such as Pd(OAc)
- the resulting compound can then be purified using techniques known in the art such as preparative HPLC and characterized.
- 7 and 9 aminomethyl tetracyclines may be synthesized using reagents such as hydroxymethyl-carbamic acid benzyl ester.
- alkenyl includes unsaturated aliphatic groups, including straight-chain alkenyl groups, branched-chain alkenyl groups, cycloalkenyl (alicyclic) groups, alkenyl substituted cycloalkyl or cycloalkenyl groups, and cycloalkenyl substituted alkyl or alkenyl groups.
- alkenyl further includes alkenyl groups, which can further include oxygen, nitrogen, sulfur or phosphorous atoms replacing one or more carbons of the hydrocarbon backbone, e.g., oxygen, nitrogen, sulfur or phosphorous atoms.
- a straight chain or branched chain alkenyl group has 10 or fewer carbon atoms in its backbone (e.g., C -CJO for straight chain, C3-C1 Q for branched chain), and more preferably 6 or fewer.
- preferred cycloalkenyl groups have from 4-7 carbon atoms in their ring structure, and more preferably have 5 or 6 carbons in the ring structure, e.g., cyclopentene or cyclohexene.
- alkyl includes saturated aliphatic groups, including straight-chain alkyl groups, branched-chain alkyl groups, cycloalkyl (alicyclic) groups, alkyl substituted cycloalkyl groups, and cycloalkyl substituted alkyl groups.
- alkyl further includes alkyl groups, which can further include oxygen, nitrogen, sulfur or phosphorous atoms replacing one or more carbons of the hydrocarbon backbone, e.g., oxygen, nitrogen, sulfur or phosphorous atoms.
- a straight chain or branched chain alkyl has 10 or fewer carbon atoms in its backbone (e.g., C1 -C ⁇ Q for straight chain, C3-C1 Q for branched chain), and more preferably 6 or fewer.
- preferred cycloalkyls have from 4-7 carbon atoms in their ring structure, and more preferably have 5 or 6 carbons in the ring structure.
- alkyl includes both "unsubstituted alkyls" and “substituted alkyls”, the latter of which refers to alkyl moieties having substituents replacing a hydrogen on one or more carbons of the hydrocarbon backbone.
- substituents can include, for example, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoro
- aryl includes aryl groups, including 5- and 6-membered single-ring aromatic groups that may include from zero to four heteroatoms, for example, benzene, pyrrole, furan, thiophene, imidazole, benzoxazole, benzothiazole, triazole, tetrazole, pyrazole, pyridine, pyrazine, pyridazine and pyrimidine, and the like.
- Aryl groups also include polycyclic fused aromatic groups such as naphthyl, quinolyl, indolyl, and the like.
- aryl groups having heteroatoms in the ring structure may also be referred to as "aryl heterocycles", “heteroaryls” or “heteroaromatics”.
- the aromatic ring can be substituted at one or more ring positions with such substituents as described above, as for example, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylthiocarbonyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, s
- alkenyl and alkynyl include unsaturated aliphatic groups analogous in length and possible substitution to the alkyls described above, but that contain at least one double or triple bond, respectively.
- substituents of alkynyl groups include, for example alkyl, alkenyl (e.g., cycloalkenyl, e.g., cyclohenxenyl), and aryl groups.
- lower alkyl as used herein means an alkyl group, as defined above, but having from one to three carbon atoms in its backbone structure. Likewise, “lower alkenyl” and “lower alkynyl” have similar chain lengths.
- alkoxyalkyl polyaminoalkyl
- thioalkoxyalkyl include alkyl groups, as described above, which further include oxygen, nitrogen or sulfur atoms replacing one or more carbons of the hydrocarbon backbone, e.g., oxygen, nitrogen or sulfur atoms.
- polycyclyl or “polycyclic radical” refer to two or more cyclic rings (e.g., cycloalkyls, cycloalkenyls, aryls and/or heterocyclyls) in which two or more carbons are common to two adjoining rings, e.g., the rings are "fused rings". Rings that are joined through non-adjacent atoms are termed "bridged" rings.
- Each of the rings of the polycycle can be substituted with such substituents as described above, as for example, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, sulfonato, sulfamoyl,
- heteroatom includes atoms of any element other than carbon or hydrogen. Preferred heteroatoms are nitrogen, oxygen, sulfur and phosphorus.
- alkylsulfinyl include groups which have one or more sulfinyl (SO) linkages, typically 1 to about 5 or 6 sulfinyl linkages.
- Advantageous alkylsulfinyl groups include groups having 1 to about 12 carbon atoms, preferably from 1 to about 6 carbon atoms.
- alkylsulfonyl includes groups which have one or more sulfonyl (SO2) linkages, typically 1 to about 5 or 6 sulfonyl linkages.
- Advantageous alkylsulfonyl groups include groups having 1 to about 12 carbon atoms, preferably from 1 to about 6 carbon atoms.
- alkanoyl includes groups having 1 to about 4 or 5 carbonyl groups.
- aroyl includes aryl groups, such as phenyl and other carbocyclic aryls, which have carbonyl substituents.
- alkaroyl includes aryl groups with alkylcarbonyl substituents, e.g., phenylacetyl.
- the structures of some of the substituted tetracycline compounds used in the methods and compositions of the invention include asymmetric carbon atoms.
- the isomers arising from the chiral atoms e.g., all enantiomers and diastereomers are included within the scope of this invention, unless indicated otherwise. Such isomers can be obtained in substantially pure form by classical separation techniques and by stereochemically controlled synthesis.
- the invention pertains to methods for treating fungal associated disorder in a subject.
- the method includes administering to the subject an effective amount of a substituted tetracycline compound in combination with an antifungal agent such that the subject is treated for the fungal associated disorder.
- the language "effective amount" of a substituted tetracycline compound and/or an antifungal agent is that amount necessary or sufficient to inhibit the growth of fungus, or treat a fungus associated disorder, e.g., in a subject, e.g., prevent the various morphological and somatic symptoms of a fungal associated disorder.
- the effective amount can vary depending on such factors as the size and weight of the subject, the type of disorder, or the particular substituted tetracycline compound and/or antifungal agent.
- the choice of the substituted tetracycline compound and/or antifungal agent can affect what constitutes an "effective amount".
- One of ordinary skill in the art would be able to study the aforementioned factors and make the determination regarding the effective amount of the substituted tetracycline compound and/or antifungal agent without undue experimentation.
- An in vitro assay as described in Example 2 below or an assay similar thereto (e.g., differing in choice of fungus) also can be used to determine an "effective amount" of a substituted tetracycline compound and/or antifungal agent.
- the ordinarily skilled artisan would select an appropriate amount of a substituted tetracycline compound for use in the aforementioned in vitro assay.
- subject any organism which may benefit from the inhibition of a fungus or which is capable of having a fungal associated disorder.
- subjects include not only animals, such as mammals, birds, fish, etc., but plants which may be adversely effected by the presence of a fungus.
- mammal includes, but is not limited to, ruminants (e.g., cattle and goats), mice, rats, hamsters, dogs, cats, horses, pigs, sheep, lions, tigers, bears, monkeys, chimpanzees, and, in a preferred embodiment, humans.
- the mammal may be immunocompetent or immunocompromised, e.g., suffering from an immunodeficiency.
- the mammal may have AIDS or may have previously or concurrently undergone chemotherapy.
- the mammal may be elderly or young.
- the mammal may or may not be suffering from a fungal associated disorder.
- the tetracycline compounds may be administered to a mammal susceptible to a fungal associated disorder to prevent the occurrence of the disorder.
- the language "fungal associated disorder” includes disorders which are related to the presence of fungus in a subject. Examples of fungal associated disorders in animals include topical fungal infections caused by, e.g., Candida, and dermatophytes such as Trichophyton, Microsporum or Epidermophyton, or in mucosal infections caused by Candida albicans (e.g., oral thrush and vaginal candidiasis).
- the substituted tetracycline compounds of the invention are also useful for treatment of systemic fungal infections caused by, for example, Candida albicans, Cryptococcus neoformans, Aspergillus flavus, Aspergillus fumigatus, Coccidioides, Paracoccidioides, Histoplasma or Blastomyces.
- the substituted tetracycline compounds of the invention are particularly useful for treating fungal infections in immunocompromised patients such as patients with viral infections such as AIDS, CMN, and influenza, cancer patients receiving chemotherapy or radiotherapy, transplant patients receiving antirejection agents, and patients that have received toxic chemicals, metals and radiation exposure.
- fungal associated disorders include aspergiUosis, candidosis, chromomycosis, coccidioidiocycosis, cryptocococcosis, entomophthoromycosis, epizootic lymphangitis, geotrichosis, histoplasmosis, mucormycosis, mycetoma, north american blastomycosis, oomycosis, paecilimycosis, penicilliosis, rhinosporidiosis, and sprotrichiosis in animals.
- the substituted tetracycline compounds of the invention can be included in feed for the livestock, such that normal consumption of said feed provides about 1 mg to about 200 mg of at least one of the substituted tetracycline compounds of the invention per kg of animal per day.
- the term "in combination with" an antifungal agent is intended to include simultaneous administration of the substituted tetracycline compound and the antifungal agent, administration of the antifungal agent first, followed by the substituted tetracycline compound and administration of the substituted tetracycline compound first, followed by the antifungal agent.
- the antifungal agent can be administered by the same or one or more different routes than the tetracycline.
- the invention also pertains to a method for treating a fungal associated disorder in a mammal.
- the method includes administering to a mammal a synergistically effective amount of a substituted tetracycline compound in combination with an effective amount amphotericin B, such that said mammal is treated for said fungal associated disorder.
- the tetracycline compound is a compound of formula (I).
- the tetracycline compound is a tetracycline compound shown in Table 2.
- the invention also pertains to pharmaceutical compositions comprising a synergistically effective amount of a substituted tetracycline compound, an effective amount of an antifungal agent, and, optionally, a pharmaceutically acceptable carrier.
- the term "synergistically effective amount” is the amount of a substituted tetracycline compound of the invention necessary to increase the antifungal activity of the antifungal agent, such that the fungal associated disorder is treated.
- the pharmaceutically acceptable carrier may be formulated such that it releases one or more of the active components over a desireable length of time, e.g., time release, by methods known in the art.
- one or more compounds of the invention may be administered alone to a subject, or more typically a compound of the invention will be administered as part of a pharmaceutical composition in mixture with conventional excipient, i.e., pharmaceutically acceptable organic or inorganic carrier substances suitable for parenteral, oral or other desired administration and which do not deleteriously react with the active compounds and are not deleterious to the recipient thereof.
- Suitable pharmaceutically acceptable carriers include but are not limited to water, salt solutions, alcohol, vegetable oils, polyethylene glycols, gelatin, lactose, amylose, magnesium stearate, talc, silicic acid, viscous paraffin, perfume oil, fatty acid monoglycerides and diglycerides, petroethral fatty acid esters, hydroxymethylcellulose, polyvinylpyrrolidone, etc.
- Substituted tetracycline compounds and antifungal agents may be administered to a subject in a protonated and water-soluble form, e.g., as a pharmaceutically acceptable salt of an organic or inorganic acid, e.g., hydrochloride, sulfate, hemi-sulfate, phosphate, nitrate, acetate, oxalate, citrate, maleate, mesylate, etc.
- an organic or inorganic acid e.g., hydrochloride, sulfate, hemi-sulfate, phosphate, nitrate, acetate, oxalate, citrate, maleate, mesylate, etc.
- a pharmaceutically acceptable salt of an organic or inorganic base can be employed such as an ammonium salt, or salt of an organic amine, or a salt of an alkali metal or alkaline earth metal such as a potassium, calcium or sodium salt.
- Therapeutic compounds can be administered to a subject in accordance with the invention by any of a variety of routes.
- Topical including transdermal, buccal or sublingual
- oral including parenteral (including intraperitoneal, subcutaneous, intravenous, intradermal or intramuscular injection) are generally preferred.
- compositions of a similar type may also be employed as fillers in gelatin capsules; preferred materials in this connection also include lactose or milk sugar as well as high molecular weight polyethylene glycols.
- preferred materials in this connection also include lactose or milk sugar as well as high molecular weight polyethylene glycols.
- the active ingredient may be combined with various sweetening or flavoring agents, coloring matter or dyes, and, if so desired, emulsifying and/or suspending agents as well, together with such diluents as water, ethanol, propylene glycol, glycerin and various like combinations thereof.
- compositions for parenteral application, particularly suitable are solutions, preferably oily or aqueous solutions as well as suspensions, emulsions, or implants, including suppositories.
- Therapeutic compositions will be formulated in sterile form in multiple or single dose formats such as being dispersed in a fluid carrier such as sterile physiological saline or 5% saline dextrose solutions commonly used with injectables.
- tablets, dragees or capsules having talc and/or carbohydrate carrier binder or the like are particularly suitable, the carrier preferably being lactose and/or corn starch and/or potato starch.
- a syrup, elixir or the like can be used wherein a sweetened vehicle is employed.
- Sustained release compositions can be formulated including those wherein the active component is protected with differentially degradable coatings, e.g., by microencapsulation, multiple coatings, etc.
- the substituted tetracycline compound and antifungal agents can be suitably admixed in a pharmacologically inert topical carrier such as a gel, an ointment, a lotion or a cream.
- topical carriers include water, glycerol, alcohol, propylene glycol, fatty alcohols, triglycerides, fatty acid esters, or mineral oils.
- Other possible topical carriers are liquid petrolatum, isopropylpalmitate, polyethylene glycol, ethanol 95%, polyoxyethylene monolauriate 5% in water, sodium lauryl sulfate 5% in water, and the like.
- materials such as anti-oxidants, humectants, viscosity stabilizers and the like also may be added if desired.
- compounds of the invention for treatment can be administered to a subject in dosages used in prior tetracycline therapies. See, for example, the Physicians ' Desk Reference.
- a suitable effective dose of one or more compounds of the invention will be in the range of from 0.01 to 100 milligrams per kilogram of body weight of recipient per day, preferably in the range of from 0.1 to 50 milligrams per kilogram body weight of recipient per day, more preferably in the range of 1 to 20 milligrams per kilogram body weight of recipient per day.
- the desired dose is suitably administered once daily, or several sub-doses, e.g. 2 to 5 sub-doses, are administered at appropriate intervals through the day, or other appropriate schedule.
- the substituted tetracycline compounds and antifungal agents of the present invention can also be used in agricultural compositions, for example, compositions for plants and seeds to treat or prevent a variety of plant pathogenic fungi, including rusts, mildews, and molds.
- the compounds of the present invention are dispensed in the form of dusting powders, granules, seed dressings, aqueous solutions, dispersions or emulsions, dips, sprays, aerosols or smokes.
- Compositions may also be supplied in the form of dispersible powders, granules or grains, or concentrates for dilution prior to use.
- compositions may contain such conventional carriers, diluents or adjuvants as are known and acceptable in agriculture and horticulture, and they are manufactured in accordance with conventional procedures.
- the compositions typically contain from 0.01 to 10 wt %, preferably 0.1 to 1 wt. % of the active ingredient.
- the compositions may also incorporate other active ingredients, for example, compounds having herbicidal or insecticidal activity or a further fungicide.
- the compounds and compositions can be applied in a number of ways, for example, they can be applied directly to the plant foliage, stems, branches, seeds or roots or to the soil or other growing medium and they may be used not only to eradicate disease, but also prophylactically to protect the plants or seeds from attack. For field use, likely application rates of active ingredient are about 100 to 10,000 g/acre.
- the invention also pertains to methods of killing fungus, by contacting the fungus with a synergistically effective amount of a substituted tetracycline compound and a effective amount of an antifungal agent, such that said fungus is killed.
- the present invention is further illustrated by the following examples. These examples are provided to aid in the understanding of the invention and are not to be construed as limitations thereof. Exemplification of the Invention
- Solvent systems used were as follows: 50:50:5 CHCl 3 /MeOH/5% Na 2 EDTA (lower phase) (I), 65:20:5, CHCl 3 /MeOH/Na 2 EDTA (lower phase) (II).
- Visualization of TLC was accomplished by 0.5% aqueous Fast Blue BB salt and heating at 130 °C for 5 minutes.
- Analytical HPLC was performed on a Waters Bondapak C18 reverse phase column by using two Varian SD 100 HPLC pumps at a 1.6 mL/min flow rate controlled by software. Detection was by UN absorption with Model 441 absorbance detector operating at 280 nn .
- the 7-iodo isomer of sancycline was purified by treating the 7-iodo product with activated charcoal., filtering through Celite, and subsequent removal of the solvent in vacuo to produce the 7-isomer compound as a pure yellow solid in 75% yield.
- the solvent was removed in vacuo to yield the product plus salts.
- the salts were removed by extraction into 50:25:25 water, butanol, ethyl acetate and dried in vacuo. This solid was dissolved in MeOH and the HCI salt made by bubbling in HCI gas.
- the solvent was removed in vacuo to yield the product plus salts.
- the salts were removed by extraction into 50:25:25 water, butanol, ethyl acetate and dried in vacuo. This solid was dissolved in MeOH and the HCI salt made by bubbling in HCI gas. The solvent was removed to produce the product.
- the preparative liquid chromatography used a C 18 stationary phase with eluent A: 0.1% TFA in water and eluent B: 0.1% TFA in acetonitrile.
- the resulting compound was determined to be compound BE as determined by standard techniques.
- the reaction was suctioned filtered through a pad of diatomaceous earth and washed the pad with DMF. The filtrates were reduced to an oil under vacuum and residue treated with t-butylmethyl ether. Crude material was purified via reverse phase HPLC on DVB utilizing a gradient of water and methanol/acetonitrile containing 1.0% trifluoroacetic acid.
- the mixture was placed in a hydrogenator under 50 psi hydrogen pressure. The reaction was completed in ⁇ 8 hours. The catalyst was filtered off, and the resulting solution was concentrated.
- the crude product was purified by preparative liquid chromatography using a C 18 stationary phase with eluent A: 0.1% TFA in water and eluent B: 0.1% TFA in acetonitrile. The combined clean fractions are concentrated and hydrochloric acid saturated isopropanol added. The pure product is precipitated by addition of diethylether and filtered off.
- the crude material was purified by precipitating it with ether (200 ml). The yellow precipitate was filtered and purified using preparative HPLC. The hydrochloride salt was made by disolving the material in MeOH/HCl and evaporating to dryness. The identity of the resulting solid was confirmed using HPLC, MS, and NMR.
- the brown solid (9-(2'formyl furanyl)-minocycline, 1 g) was dissolved in 20 mL of methanol and acetic acid (2 mL) and hydroxy ethyl piperazine (1 mL) was added and stirred for ten minutes at room temperature. The reaction was quenched with ether (200 mL), and the organic layer was then washed and concentrated to yield a brown oil. The brown oil was the dissolved in methanol (10 mL) and water. The mixture was the chromatographed using a CH 3 CN gradient to yield the product, 2-[4-(9-Minocyclin-2-yl-furan-2-ylmethyl)- piperazin-1-yl] -ethanol. The product was confirmed using MS, NMR, and HPLC.
- the brown solid (9-(2'formyl furanyl)-minocycline, 1 g) was dissolved in 20 mL of methanol and acetic acid (2 mL) and N'-benzyl-N,N-dimethyl ethylenediamine (1 mL) was added and stirred for ten minutes at room temperature. The reaction was quenched with ether (200 mL), and the organic layer was then washed and concentrated to yield a brown oil. The brown oil was the dissolved in methanol (10 mL) and water.
- the mixture was the chromatographed using a CH 3 CN gradient to yield the product, N-Benzyl-N',N'-dimethyl-N-(5- minocyclin-9-yl-furan-2-ylmethyl)-ethane-l,2-diamine.
- the product was confirmed using MS, NMR, and HPLC.
- Synergetic antifungal activity of the substituted tetracycline compounds was determined by a broth microdillution technique following NCCLS (1997) Standards. Assays were setup using a Tecan Genesis robotic workstation. All drugs were dissolved in DMSO and diluted appropriately. Drug concentration ranged from 0.125 to 64 ⁇ g/mL in 2 fold serial dilutions. Each tetracycline was tested at 10 concentrations ranging from 0.125 to 64 ⁇ g/mL. The compounds were tested for their antifungal activity against Candida albicans (ATCC#90028). Amphotericin B was added to all wells of the plate at a concentration of 10 fold less than the amphotericin B MIC (0.5 ⁇ g/mL).
- strains tested include those listed in Table 1.
- Candida lusitaniae ATCC 24347 Candida lusitaniae ATCC 24347
- fractional inhibitory concentrations are a measure of the enhancement of the amphotericin B antifungal activity.
- Compounds which exhibit FIC's in the range of 0.063- 0.125 allows for a 8-10 fold reduction in the effective amount of amphotericin required for antifungal activity.
- COS-1 and CHO Cell suspensions were prepared, seeded into 96-well tissue culture treated black-walled microtiter plates (density determined by cell line), and incubated overnight at 37°C, in 5% CO 2 and approximately 95% humidity. The following day serial dilutions of drug were prepared under sterile conditions and transferred to cell plates.
- the final cell density should be approximately 5xl0 5 CFU/ml. These plates are incubated at 35°C in an ambient air incubator for approximately 18 hr. The plates are read with a microplate reader and are visually inspected when necessary.
- the MIC is defined as the lowest concentration of the tetracycline compound that inhibits growth.
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002570991A JP2005504722A (en) | 2001-03-14 | 2002-03-14 | Substituted tetracycline compounds as synergistic antifungal agents |
CA002440757A CA2440757A1 (en) | 2001-03-14 | 2002-03-14 | Substituted tetracycline compounds as synergistic antifungal agents |
EP02750617A EP1381372A2 (en) | 2001-03-14 | 2002-03-14 | Substituted tetracycline compounds as synergistic antifungal agents |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US27589901P | 2001-03-14 | 2001-03-14 | |
US60/275,899 | 2001-03-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002072031A2 true WO2002072031A2 (en) | 2002-09-19 |
WO2002072031A3 WO2002072031A3 (en) | 2003-11-13 |
Family
ID=23054298
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2002/007829 WO2002072031A2 (en) | 2001-03-14 | 2002-03-14 | Substituted tetracycline compounds as synergistic antifungal agents |
Country Status (5)
Country | Link |
---|---|
US (3) | US7045507B2 (en) |
EP (1) | EP1381372A2 (en) |
JP (1) | JP2005504722A (en) |
CA (1) | CA2440757A1 (en) |
WO (1) | WO2002072031A2 (en) |
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US7045507B2 (en) | 2006-05-16 |
CA2440757A1 (en) | 2002-09-19 |
JP2005504722A (en) | 2005-02-17 |
EP1381372A2 (en) | 2004-01-21 |
US7960366B2 (en) | 2011-06-14 |
US20110245501A1 (en) | 2011-10-06 |
US20030166585A1 (en) | 2003-09-04 |
WO2002072031A3 (en) | 2003-11-13 |
US20050070510A1 (en) | 2005-03-31 |
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