WO2002056004A8 - Thin film electrophoresis apparatus and method - Google Patents

Thin film electrophoresis apparatus and method

Info

Publication number
WO2002056004A8
WO2002056004A8 PCT/US2002/000985 US0200985W WO02056004A8 WO 2002056004 A8 WO2002056004 A8 WO 2002056004A8 US 0200985 W US0200985 W US 0200985W WO 02056004 A8 WO02056004 A8 WO 02056004A8
Authority
WO
WIPO (PCT)
Prior art keywords
data
thin film
electrophoresis apparatus
electrophoresis
film electrophoresis
Prior art date
Application number
PCT/US2002/000985
Other languages
French (fr)
Other versions
WO2002056004A2 (en
WO2002056004A3 (en
Inventor
Andras Guttman
Bart Wanders
Phil Alei
Original Assignee
Syngenta Participations Ag
Andras Guttman
Bart Wanders
Phil Alei
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag, Andras Guttman, Bart Wanders, Phil Alei filed Critical Syngenta Participations Ag
Priority to CA002434469A priority Critical patent/CA2434469A1/en
Priority to EP02705777A priority patent/EP1412733A2/en
Priority claimed from US10/047,461 external-priority patent/US20030132117A1/en
Publication of WO2002056004A2 publication Critical patent/WO2002056004A2/en
Publication of WO2002056004A8 publication Critical patent/WO2002056004A8/en
Publication of WO2002056004A3 publication Critical patent/WO2002056004A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Electrochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

High throughput electrophoresis having a plurality of samples migrating through a highly resistive separatory media under an electric field. Multiple electrophoresis runs are conducted using a single separatory media. Automated methods of data analysis yields quantitative and qualitative data using optical densitometer or other detection systems. Controlled operating temperatures and small geometry reduces dispersion inaccuracy in banded data.
PCT/US2002/000985 2001-01-12 2002-01-14 Thin film electrophoresis apparatus and method WO2002056004A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002434469A CA2434469A1 (en) 2001-01-12 2002-01-14 Thin film electrophoresis apparatus and method
EP02705777A EP1412733A2 (en) 2001-01-12 2002-01-14 Thin film electrophoresis apparatus and method

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US75998901A 2001-01-12 2001-01-12
US09/759,989 2001-01-12
US10/047,461 2002-01-14
US10/047,461 US20030132117A1 (en) 2002-01-14 2002-01-14 Thin film electrophoresis apparatus and method

Publications (3)

Publication Number Publication Date
WO2002056004A2 WO2002056004A2 (en) 2002-07-18
WO2002056004A8 true WO2002056004A8 (en) 2003-01-16
WO2002056004A3 WO2002056004A3 (en) 2004-02-19

Family

ID=26725040

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2002/000993 WO2002055189A2 (en) 2001-01-12 2002-01-14 Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics
PCT/US2002/000985 WO2002056004A2 (en) 2001-01-12 2002-01-14 Thin film electrophoresis apparatus and method

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2002/000993 WO2002055189A2 (en) 2001-01-12 2002-01-14 Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics

Country Status (3)

Country Link
AU (1) AU2002239908A1 (en)
CA (1) CA2434469A1 (en)
WO (2) WO2002055189A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005029062A2 (en) * 2003-09-22 2005-03-31 Shimadzu Corporation Electrophoretic member electrophoretic device electrophoretic method and sample dispensing probe
GB2439233B (en) * 2003-09-22 2008-04-23 Shimadzu Corp An electrophoretic separation method
CN100447567C (en) * 2004-09-22 2008-12-31 杭州生源医疗保健技术开发有限公司 Composite ionic membrane micro electric chromatography
US8206974B2 (en) 2005-05-19 2012-06-26 Netbio, Inc. Ruggedized apparatus for analysis of nucleic acid and proteins
EP2137523A1 (en) 2007-04-04 2009-12-30 Network Biosystems, Inc. Plastic microfluidic separation and detection platforms
US8067730B2 (en) 2007-07-20 2011-11-29 The George Washington University Laser ablation electrospray ionization (LAESI) for atmospheric pressure, In vivo, and imaging mass spectrometry
US8901487B2 (en) 2007-07-20 2014-12-02 George Washington University Subcellular analysis by laser ablation electrospray ionization mass spectrometry
US7964843B2 (en) 2008-07-18 2011-06-21 The George Washington University Three-dimensional molecular imaging by infrared laser ablation electrospray ionization mass spectrometry
JP2012529908A (en) 2009-06-15 2012-11-29 ネットバイオ・インコーポレーテッド Improved method for quantification of forensic DNA
EP2732457A4 (en) 2011-07-14 2015-09-16 Univ George Washington Plume collimation for laser ablation electrospray ionization mass spectrometry
GB2502953B (en) * 2012-05-24 2017-02-08 Imp Innovations Ltd Catalytic converter substrate
NL2009191C2 (en) * 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
GB2517951A (en) 2013-09-05 2015-03-11 Imp Innovations Ltd A substrate and a method of manufacturing a substrate
US10115577B1 (en) * 2017-09-07 2018-10-30 California Institute Of Technology Isotope ratio mass spectrometry

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4588491A (en) * 1984-02-27 1986-05-13 International Biotechnologies, Inc. Horizontal gel electrophoresis device
US5137613A (en) * 1990-12-20 1992-08-11 Wisconsin Alumni Research Foundation Horizontal gel electrophoresis apparatus
FR2671290B1 (en) * 1991-01-04 1993-04-16 Sebia Sa DEVICE FOR APPLYING BIOLOGICAL SAMPLES TO AN ELECTROPHORESIS PLATE.
US5338426A (en) * 1992-08-28 1994-08-16 Applied Biosystems, Inc. Horizontal polyacrylamide gel electrophoresis
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5990373A (en) * 1996-08-20 1999-11-23 Kansas State University Research Foundation Nanometer sized metal oxide particles for ambient temperature adsorption of toxic chemicals
US6057488A (en) * 1998-09-15 2000-05-02 Nantek, Inc. Nanoparticles for the destructive sorption of biological and chemical contaminants
AU4807600A (en) * 1999-04-26 2000-11-10 Mj Research, Inc. Electrophoresis assembly and method of casting electrophoresis gels
GB2353866B (en) * 1999-05-06 2003-12-03 Univ Southampton Gel electrophoresis

Also Published As

Publication number Publication date
CA2434469A1 (en) 2002-07-18
WO2002056004A2 (en) 2002-07-18
WO2002055189A3 (en) 2004-10-28
WO2002055189A2 (en) 2002-07-18
WO2002056004A3 (en) 2004-02-19
AU2002239908A1 (en) 2002-07-24

Similar Documents

Publication Publication Date Title
WO2002056004A3 (en) Thin film electrophoresis apparatus and method
Loose et al. Real-time selective sequencing using nanopore technology
Bonner et al. Laser capture microdissection: molecular analysis of tissue
WO2005114930A3 (en) Method and apparatus for identifying proteins in mixtures
CA2192835A1 (en) Method and Apparatus for Performing Automated Analysis
US20070199821A1 (en) Automated two-dimensional gel electrophoresis
AU7398996A (en) Multiplexed analysis of clinical specimens apparatus and method
ES2160236T3 (en) IMPROVEMENTS IN OR RELATED TO SAMPLING PROCESSING.
WO2005027015A3 (en) Method and system for quantitatively analyzing biological samples
DE69530796T2 (en) METHOD FOR PRODUCING CROSS-CROSSING, NON-CUTTING CHANNELS IN DIELECTRIC SUBSTRATE MATRICES, DIELECTRIC SUBSTRATE MATRICES AND THEIR USE
EP0244751A3 (en) Automated multiple stream analysis system
EP1540330A4 (en) Method and staining reagents for staining hematology samples in an automated staining apparatus
JPH087185B2 (en) Method for preparing and separating macromolecules
WO2003052142A3 (en) Analysis and detection of multiple target sequences using circular probes
WO2004012231A3 (en) Method and apparatus for control of mobility-based ion species identification
Lenz et al. Separation methodology to improve proteome coverage depth
Swart et al. Hit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments
DE60232025D1 (en) INTEGRATED MICROARRAY SYSTEM AND METHOD THEREFOR
WO2001075156A1 (en) Microbe identifying method, microbe identifying apparatus, method for creating database for microbe identification, microbe identifying program, and recording medium on which the same is recorded
EP4163803A4 (en) Sample data annotation system, method, and related device
WO2003021230A3 (en) High-throughput electrophysiological measurement system
WO2003046513A3 (en) Method of two-dimensional gel electrophoresis for protein display
Wei et al. High-throughput DNA fragment analysis with the cePRO 9600 [TM] system
AU2002347462A1 (en) A computer system and method using mass spectrometry data and a protein database for identifying unknown proteins
Sungu et al. Skin punch biopsy sectioning: before or after tissue processing?

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: C1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: C1

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: PAT. BUL. 29/2002 UNDER (30) REPLACE "NOT FURNISHED, 20020104, US" BY "10/047461, 20020114, US"

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2002239906

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2434469

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2002705777

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 2002705777

Country of ref document: EP

NENP Non-entry into the national phase in:

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2002705777

Country of ref document: EP