WO2002031203A3 - High throughput or capillary-based screening for a bioactivity or biomolecule - Google Patents

High throughput or capillary-based screening for a bioactivity or biomolecule Download PDF

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Publication number
WO2002031203A3
WO2002031203A3 PCT/US2001/031806 US0131806W WO0231203A3 WO 2002031203 A3 WO2002031203 A3 WO 2002031203A3 US 0131806 W US0131806 W US 0131806W WO 0231203 A3 WO0231203 A3 WO 0231203A3
Authority
WO
WIPO (PCT)
Prior art keywords
organisms
based screening
mixed population
library
devices
Prior art date
Application number
PCT/US2001/031806
Other languages
French (fr)
Other versions
WO2002031203A2 (en
WO2002031203A9 (en
Inventor
Jay M Short
Martin Keller
William Michael Lafferty
Original Assignee
Diversa Corp
Jay M Short
Martin Keller
William Michael Lafferty
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/738,871 external-priority patent/US20010041333A1/en
Priority claimed from US09/790,321 external-priority patent/US20020048809A1/en
Priority claimed from US09/894,956 external-priority patent/US20020015997A1/en
Priority to CA002393374A priority Critical patent/CA2393374A1/en
Priority to AU11642/02A priority patent/AU1164202A/en
Priority to MXPA02005717A priority patent/MXPA02005717A/en
Application filed by Diversa Corp, Jay M Short, Martin Keller, William Michael Lafferty filed Critical Diversa Corp
Priority to JP2002534567A priority patent/JP2005501217A/en
Priority to IL15002001A priority patent/IL150020A0/en
Priority to EP01979708A priority patent/EP1364052A2/en
Publication of WO2002031203A2 publication Critical patent/WO2002031203A2/en
Publication of WO2002031203A9 publication Critical patent/WO2002031203A9/en
Publication of WO2002031203A3 publication Critical patent/WO2002031203A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

Provided is a method of screening or enriching a sample containing polynucleotides from a mixed population of organisms. The method includes creating a DNA library from a plurality of nucleic acid sequences of a mixed population of organisms and separating clones containing a polynucleotide sequence of interest on an analyzer detects a detectable molecule on a probe or bioactive substrate. The analyzer includes FACS devices, SQUID devices and MSC devices. The separated or enrich library can then be further process by activity based screening or sequence based screening. In addition, the enriched sequence can be compared to a database and to identify sequences in the database which have homology to a clone in the library thereby obtaining a nucleic acid profile of the mixed population of organisms.
PCT/US2001/031806 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule WO2002031203A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP01979708A EP1364052A2 (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule
IL15002001A IL150020A0 (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule
JP2002534567A JP2005501217A (en) 2000-10-10 2001-10-10 High-throughput or capillary-based screening for bioactivity or biomolecules
AU11642/02A AU1164202A (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule
MXPA02005717A MXPA02005717A (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule.
CA002393374A CA2393374A1 (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US68543200A 2000-10-10 2000-10-10
US09/685,432 2000-10-10
US09/738,871 US20010041333A1 (en) 1997-06-16 2000-12-15 High throughput screening for a bioactivity or biomolecule
US09/738,871 2000-12-15
US09/790,321 US20020048809A1 (en) 1997-06-16 2001-02-21 Capillary array-based sample screening
US09/790,321 2001-02-21
US09/894,956 US20020015997A1 (en) 1997-06-16 2001-06-27 Capillary array-based sample screening
US09/894,956 2001-06-27
US30910101P 2001-07-31 2001-07-31
US60/309,101 2001-07-31

Publications (3)

Publication Number Publication Date
WO2002031203A2 WO2002031203A2 (en) 2002-04-18
WO2002031203A9 WO2002031203A9 (en) 2003-07-03
WO2002031203A3 true WO2002031203A3 (en) 2003-09-25

Family

ID=27540945

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/031806 WO2002031203A2 (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule

Country Status (7)

Country Link
EP (1) EP1364052A2 (en)
JP (1) JP2005501217A (en)
AU (1) AU1164202A (en)
CA (1) CA2393374A1 (en)
IL (1) IL150020A0 (en)
MX (1) MXPA02005717A (en)
WO (1) WO2002031203A2 (en)

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Also Published As

Publication number Publication date
EP1364052A2 (en) 2003-11-26
MXPA02005717A (en) 2003-10-14
JP2005501217A (en) 2005-01-13
WO2002031203A2 (en) 2002-04-18
IL150020A0 (en) 2002-12-01
CA2393374A1 (en) 2002-04-18
WO2002031203A9 (en) 2003-07-03
AU1164202A (en) 2002-04-22

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