WO2002003787A3 - Transgenic mice containing targeted gene disruptions - Google Patents

Transgenic mice containing targeted gene disruptions Download PDF

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Publication number
WO2002003787A3
WO2002003787A3 PCT/US2001/021427 US0121427W WO0203787A3 WO 2002003787 A3 WO2002003787 A3 WO 2002003787A3 US 0121427 W US0121427 W US 0121427W WO 0203787 A3 WO0203787 A3 WO 0203787A3
Authority
WO
WIPO (PCT)
Prior art keywords
transgenic mice
targeted gene
mice containing
gene disruptions
gene
Prior art date
Application number
PCT/US2001/021427
Other languages
French (fr)
Other versions
WO2002003787A2 (en
Inventor
Keith D Allen
Michael W Leviten
Original Assignee
Deltagen Inc
Keith D Allen
Michael W Leviten
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deltagen Inc, Keith D Allen, Michael W Leviten filed Critical Deltagen Inc
Priority to AU2001271878A priority Critical patent/AU2001271878A1/en
Publication of WO2002003787A2 publication Critical patent/WO2002003787A2/en
Publication of WO2002003787A3 publication Critical patent/WO2002003787A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knockout animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Abstract

The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene functions. Specifically, the present invention provides transgenic mice comprising mutations in a target gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression and gene function, and as potential treatments for various disease states and disease conditions.
PCT/US2001/021427 2000-07-06 2001-07-06 Transgenic mice containing targeted gene disruptions WO2002003787A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001271878A AU2001271878A1 (en) 2000-07-06 2001-07-06 Transgenic mice containing targeted gene disruptions

Applications Claiming Priority (32)

Application Number Priority Date Filing Date Title
US21625100P 2000-07-06 2000-07-06
US21625800P 2000-07-06 2000-07-06
US21610900P 2000-07-06 2000-07-06
US21676800P 2000-07-06 2000-07-06
US60/216,109 2000-07-06
US60/216,251 2000-07-06
US60/216,258 2000-07-06
US60/216,768 2000-07-06
US21745000P 2000-07-10 2000-07-10
US21744900P 2000-07-10 2000-07-10
US21766000P 2000-07-10 2000-07-10
US60/217,450 2000-07-10
US60/217,449 2000-07-10
US60/217,660 2000-07-10
US22167000P 2000-07-27 2000-07-27
US22166900P 2000-07-27 2000-07-27
US22149100P 2000-07-27 2000-07-27
US60/221,670 2000-07-27
US60/221,491 2000-07-27
US60/221,669 2000-07-27
US22317200P 2000-08-07 2000-08-07
US22346000P 2000-08-07 2000-08-07
US22317000P 2000-08-07 2000-08-07
US60/223,460 2000-08-07
US60/223,170 2000-08-07
US60/223,172 2000-08-07
US24403700P 2000-10-26 2000-10-26
US24411100P 2000-10-26 2000-10-26
US60/244,037 2000-10-26
US60/244,111 2000-10-26
US30121701P 2001-06-26 2001-06-26
US60/301,217 2001-06-26

Publications (2)

Publication Number Publication Date
WO2002003787A2 WO2002003787A2 (en) 2002-01-17
WO2002003787A3 true WO2002003787A3 (en) 2003-08-14

Family

ID=27585827

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/021427 WO2002003787A2 (en) 2000-07-06 2001-07-06 Transgenic mice containing targeted gene disruptions

Country Status (2)

Country Link
AU (1) AU2001271878A1 (en)
WO (1) WO2002003787A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2374870A (en) * 2001-01-26 2002-10-30 Thromb X Nv Use of genetically modified multicellular animals in the identification of drug target proteins

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998011238A2 (en) * 1996-09-13 1998-03-19 Dana-Farber Cancer Institute Protease m, a novel serine protease
WO1998054307A1 (en) * 1997-05-30 1998-12-03 Schering Aktiengesellschaft Knockout mice and their progeny with disrupted serine protease genes
WO2000036910A1 (en) * 1998-12-22 2000-06-29 Albert Einstein College Of Medicine Msh5 ablated mice and uses therefor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998011238A2 (en) * 1996-09-13 1998-03-19 Dana-Farber Cancer Institute Protease m, a novel serine protease
WO1998054307A1 (en) * 1997-05-30 1998-12-03 Schering Aktiengesellschaft Knockout mice and their progeny with disrupted serine protease genes
WO2000036910A1 (en) * 1998-12-22 2000-06-29 Albert Einstein College Of Medicine Msh5 ablated mice and uses therefor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
WONG GUANG W ET AL: "Cloning of the human homolog of mouse transmembrane tryptase.", INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, vol. 118, no. 2-4, February 1999 (1999-02-01), pages 419 - 421, XP001100094, ISSN: 1018-2438 *
WONG GUANG W ET AL: "Identification of a new member of the tryptase family of mouse and human mast cell proteases which possesses a novel COOH-terminal hydrophobic extension.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 43, 22 October 1999 (1999-10-22), pages 30784 - 30793, XP002213262, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
AU2001271878A1 (en) 2002-01-21
WO2002003787A2 (en) 2002-01-17

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