WO2001083786A3 - Stable zymomonas mobilis xylose and arabinose fermenting strains - Google Patents

Stable zymomonas mobilis xylose and arabinose fermenting strains Download PDF

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Publication number
WO2001083786A3
WO2001083786A3 PCT/US2001/011334 US0111334W WO0183786A3 WO 2001083786 A3 WO2001083786 A3 WO 2001083786A3 US 0111334 W US0111334 W US 0111334W WO 0183786 A3 WO0183786 A3 WO 0183786A3
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WO
WIPO (PCT)
Prior art keywords
structural genes
expression
stable
bacterial genome
zymomonas mobilis
Prior art date
Application number
PCT/US2001/011334
Other languages
French (fr)
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WO2001083786B1 (en
WO2001083786A2 (en
Inventor
Min Zhang
Yat-Chen Chou
Original Assignee
Midwest Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/565,233 external-priority patent/US7354755B2/en
Priority claimed from CA002304929A external-priority patent/CA2304929C/en
Application filed by Midwest Research Inst filed Critical Midwest Research Inst
Priority to AU4992601A priority Critical patent/AU4992601A/en
Priority to JP2001580393A priority patent/JP4510355B2/en
Priority to AU2001249926A priority patent/AU2001249926B2/en
Priority to BRPI0110987-1A priority patent/BR0110987B1/en
Priority to EP01923213A priority patent/EP1278876A2/en
Publication of WO2001083786A2 publication Critical patent/WO2001083786A2/en
Priority to US10/134,655 priority patent/US7223575B2/en
Publication of WO2001083786A3 publication Critical patent/WO2001083786A3/en
Publication of WO2001083786B1 publication Critical patent/WO2001083786B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention briefly includes a transposon for stable insertion of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, and at least one promoter for expression of the structural genes in the bacterium, a pair of inverted insertion sequences, the operons contained inside the insertion sequences, and a transposase gene located outside of the insertion sequences. A plasmid shuttle vector for transformation of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, at least one promoter for expression of the structural genes in the bacterium, and at least two DNA fragments having homology with a gene in the bacterial genome to be transfomed, is also provided. The transposon and shuttle vectors are useful in constructing significantly different Zymomonas mobilis strains, according to the present invention, which are useful in the conversion of the cellulose derived pentose sugars into fuels and chemicals, using traditional fermentation technology, because they are stable for expression in a non-selection medium.
PCT/US2001/011334 2000-05-01 2001-04-06 Stable zymomonas mobilis xylose and arabinose fermenting strains WO2001083786A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU4992601A AU4992601A (en) 2000-05-01 2001-04-06 Stable zymomonas mobilis xylose and arabinose fermenting strains
JP2001580393A JP4510355B2 (en) 2000-05-01 2001-04-06 A stable xylose and arabinose fermentation strain of Thymomonas mobilis
AU2001249926A AU2001249926B2 (en) 2000-05-01 2001-04-06 Stable zymomonas mobilis xylose and arabinose fermenting strains
BRPI0110987-1A BR0110987B1 (en) 2000-05-01 2001-04-06 Transposon for stable insertion of foreign genes into a bacterial genome, plasmid vector, bacterium, and reaction mixture comprising a fermentable medium.
EP01923213A EP1278876A2 (en) 2000-05-01 2001-04-06 STABLE i ZYMOMONAS MOBILIS /i XYLOSE AND ARABINOSE FERMENTING STRAINS
US10/134,655 US7223575B2 (en) 2000-05-01 2002-04-27 Zymomonas pentose-sugar fermenting strains and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/565,233 US7354755B2 (en) 2000-05-01 2000-05-01 Stable zymomonas mobilis xylose and arabinose fermenting strains
US09/565,233 2000-05-01
CA2,304,929 2000-05-02
CA002304929A CA2304929C (en) 2000-05-02 2000-05-02 Stable zymomonas mobilis xylose and arabinose fermenting strains

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US09/565,233 Continuation-In-Part US7354755B2 (en) 2000-05-01 2000-05-01 Stable zymomonas mobilis xylose and arabinose fermenting strains

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/134,655 Continuation-In-Part US7223575B2 (en) 2000-05-01 2002-04-27 Zymomonas pentose-sugar fermenting strains and uses thereof

Publications (3)

Publication Number Publication Date
WO2001083786A2 WO2001083786A2 (en) 2001-11-08
WO2001083786A3 true WO2001083786A3 (en) 2002-06-20
WO2001083786B1 WO2001083786B1 (en) 2002-07-18

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/011334 WO2001083786A2 (en) 2000-05-01 2001-04-06 Stable zymomonas mobilis xylose and arabinose fermenting strains

Country Status (5)

Country Link
EP (1) EP1278876A2 (en)
JP (1) JP4510355B2 (en)
AU (2) AU2001249926B2 (en)
BR (1) BR0110987B1 (en)
WO (1) WO2001083786A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100374570C (en) * 2004-07-07 2008-03-12 新疆农业科学院微生物应用研究所 Electric conversion method of making shuttle plasmid guide into yellow brevibacterium

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223575B2 (en) * 2000-05-01 2007-05-29 Midwest Research Institute Zymomonas pentose-sugar fermenting strains and uses thereof
US7374939B1 (en) 2000-05-01 2008-05-20 Midwest Research Institute Method of inactivation of an end product of energy metabolism in Zymomonas mobilis
AU2003299464B2 (en) * 2000-05-01 2007-09-06 Alliance For Sustainable Energy, Llc Zymomonas pentose-sugar fermentating strains and uses thereof
BR0305217A (en) * 2002-06-26 2005-06-28 Transgenrx Inc Gene regulation in transgenic animals using a transposon-based vector
EP1752532A1 (en) 2005-08-09 2007-02-14 Helmholtz-Zentrum für Infektionsforschung GmbH Extracellular polyhydroxyalkanoates produced by genetically engineered microorganisms
US7741119B2 (en) * 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
DE102007048134A1 (en) * 2007-10-05 2009-04-09 Evocatal Gmbh Cloning, integration and expression of a gene cluster comprises using gene cassettes comprising sequences that mediate the transfer, integration and expression of a flanked nucleic acid
ES2385857T3 (en) * 2007-10-30 2012-08-01 E. I. Du Pont De Nemours And Company Zymomonas with increased ethanol production in medium containing concentrated sugars and acetate
JP2011515067A (en) * 2007-10-30 2011-05-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー Method for producing ethanol from a medium containing xylose using a recombinant zymomonas strain with reduced expression of himA
EP2271743A2 (en) * 2008-03-27 2011-01-12 E. I. du Pont de Nemours and Company Zymomonas with improved xylose utilization
WO2010036978A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of growth hormone
WO2010036976A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
EP2417263B1 (en) 2009-04-09 2015-09-23 ProteoVec Holding L.L.C. Production of proteins using transposon-based vectors
US20130157332A1 (en) * 2011-12-20 2013-06-20 E I Du Pont De Nemours And Company Gene inactivation allowing immediate growth on xylose medium by engineered zymomonas

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997042307A1 (en) * 1996-05-06 1997-11-13 Purdue Research Foundation Stable recombinant yeasts for fermenting xylose to ethanol
WO1998050524A1 (en) * 1997-05-06 1998-11-12 Midwest Research Institute Single zymomonas mobilis strain for xylose and arabinose fermentation
WO2001083784A2 (en) * 2000-05-01 2001-11-08 Midwest Research Institute Method of site-specific insertion in zymomonas mobilis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997042307A1 (en) * 1996-05-06 1997-11-13 Purdue Research Foundation Stable recombinant yeasts for fermenting xylose to ethanol
WO1998050524A1 (en) * 1997-05-06 1998-11-12 Midwest Research Institute Single zymomonas mobilis strain for xylose and arabinose fermentation
WO2001083784A2 (en) * 2000-05-01 2001-11-08 Midwest Research Institute Method of site-specific insertion in zymomonas mobilis

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BEJAR S ET AL: "Construction of a new strain of Streptomyces violaceoniger, having strong, constitutive and stable glucose-isomerase activity.", BIOTECHNOLOGY LETTERS, vol. 16, no. 12, 1994, pages 1259 - 1264, XP001041576, ISSN: 0141-5492 *
CHOU Y C ET AL: "Inactivation of the D-lactate dehydrogenase gene in Zymomonas mobilis through homologous recombination.", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR, vol. 99, 1999, 99th General Meeting of the American Society for Microbiology;Chicago, Illinois, USA; May 30-June 3, 1999, 1999, pages 370, XP002184799, ISSN: 1060-2011 *
LAWFORD HUGH G ET AL: "Fermentation performance assessment of a genomically integrated xylose-utilizing recombinant of Zymomonas mobilis 39676.", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 91-93, 1 April 2001 (2001-04-01), pages 117 - 131, XP001041535, ISSN: 0273-2289 *
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100374570C (en) * 2004-07-07 2008-03-12 新疆农业科学院微生物应用研究所 Electric conversion method of making shuttle plasmid guide into yellow brevibacterium

Also Published As

Publication number Publication date
BR0110987B1 (en) 2014-07-15
WO2001083786B1 (en) 2002-07-18
AU2001249926B2 (en) 2006-07-20
WO2001083786A2 (en) 2001-11-08
AU4992601A (en) 2001-11-12
BR0110987A (en) 2006-02-07
JP2004512819A (en) 2004-04-30
EP1278876A2 (en) 2003-01-29
JP4510355B2 (en) 2010-07-21

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