WO2001081619A3 - Conductivity pcr - Google Patents

Conductivity pcr Download PDF

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Publication number
WO2001081619A3
WO2001081619A3 PCT/DE2001/001025 DE0101025W WO0181619A3 WO 2001081619 A3 WO2001081619 A3 WO 2001081619A3 DE 0101025 W DE0101025 W DE 0101025W WO 0181619 A3 WO0181619 A3 WO 0181619A3
Authority
WO
WIPO (PCT)
Prior art keywords
pcr
conductivity
dna
during
quantification
Prior art date
Application number
PCT/DE2001/001025
Other languages
German (de)
French (fr)
Other versions
WO2001081619A8 (en
WO2001081619A2 (en
Inventor
Borros Arneth
Alfons Arneth
Original Assignee
Borros Arneth
Alfons Arneth
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE20007378U external-priority patent/DE20007378U1/en
Priority claimed from DE20007376U external-priority patent/DE20007376U1/en
Priority claimed from DE20013567U external-priority patent/DE20013567U1/en
Priority claimed from DE20018005U external-priority patent/DE20018005U1/en
Application filed by Borros Arneth, Alfons Arneth filed Critical Borros Arneth
Priority to AU56115/01A priority Critical patent/AU5611501A/en
Publication of WO2001081619A2 publication Critical patent/WO2001081619A2/en
Publication of WO2001081619A3 publication Critical patent/WO2001081619A3/en
Publication of WO2001081619A8 publication Critical patent/WO2001081619A8/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
    • G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors

Abstract

The invention is based on the concept of using the change in conductivity to be measured during the PCR as a marker for the progression of the PCR and for the quantification of the starting quantity of DNA. This apparatus determines the changes in conductivity 'online' during the course of a PCR by means of small microelectrodes (4) which are immersed in the PCR solution. Using this parameter, the apparatus is able to calculate the quantity of DNA or RNA before the conclusion of the PCR.
PCT/DE2001/001025 2000-04-22 2001-03-17 Conductivity pcr WO2001081619A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU56115/01A AU5611501A (en) 2000-04-22 2001-03-17 Conductivity pcr

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
DE20007378U DE20007378U1 (en) 2000-04-22 2000-04-22 Phosphate conductivity PCR
DE20007376.1 2000-04-22
DE20007378.8 2000-04-22
DE20007376U DE20007376U1 (en) 2000-04-22 2000-04-22 PCR conductivity microarrays
US20419200P 2000-05-08 2000-05-08
US60/204,192 2000-05-08
US21942100P 2000-07-20 2000-07-20
US21942200P 2000-07-20 2000-07-20
US60/219422 2000-07-20
US60/219,421 2000-07-20
DE20013567.8 2000-08-08
DE20013567U DE20013567U1 (en) 2000-08-08 2000-08-08 Conductivity PCR
DE20018005.3 2000-10-22
DE20018005U DE20018005U1 (en) 2000-10-22 2000-10-22 Sandwich PCR DNA microarray
DE10060256 2000-12-04
DE10060256.8 2000-12-04

Publications (3)

Publication Number Publication Date
WO2001081619A2 WO2001081619A2 (en) 2001-11-01
WO2001081619A3 true WO2001081619A3 (en) 2002-05-16
WO2001081619A8 WO2001081619A8 (en) 2002-06-13

Family

ID=27570759

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/001025 WO2001081619A2 (en) 2000-04-22 2001-03-17 Conductivity pcr

Country Status (1)

Country Link
WO (1) WO2001081619A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100858080B1 (en) 2002-11-12 2008-09-10 삼성전자주식회사 A method for detecting a PCR product by measuring a electrical signal
FR2941876B1 (en) * 2009-02-06 2012-12-07 Bio Rad Pasteur THERMAL VALIDATION APPARATUS, ASSEMBLY OF A DEVICE FOR PROCESSING BIOLOGICAL SAMPLES AND SUCH APPARATUS, AND METHOD FOR MANUFACTURING SUCH APPARATUS
WO2013150454A2 (en) * 2012-04-04 2013-10-10 Shama Bhat System and method for detecting and analyzing amplified deoxyribonucleic acid
DE102013010961A1 (en) 2013-07-01 2015-01-08 Borros Arneth DNA sequencing by conductivity measurement of the DNA polymerase

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0488769A2 (en) * 1990-11-29 1992-06-03 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
WO1999010530A1 (en) * 1997-08-22 1999-03-04 Molecular Sensors Limited Estimation of nucleic acid
US5891639A (en) * 1993-11-12 1999-04-06 Geron Corporation Telomerase activity assays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0488769A2 (en) * 1990-11-29 1992-06-03 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US5891639A (en) * 1993-11-12 1999-04-06 Geron Corporation Telomerase activity assays
WO1999010530A1 (en) * 1997-08-22 1999-03-04 Molecular Sensors Limited Estimation of nucleic acid

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J. P. SCHONFIELD: "a rapid semi-automated microtiter plate method for analysis and sequencing by PCR from bacterial stocks", NUCLEIC ACIDS RESEARCH, vol. 17, no. 22, 1989, pages 9498, XP001053732 *

Also Published As

Publication number Publication date
WO2001081619A8 (en) 2002-06-13
WO2001081619A2 (en) 2001-11-01

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