WO2001077350A3 - Compositions and methods for inhibiting gene expression - Google Patents

Compositions and methods for inhibiting gene expression Download PDF

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Publication number
WO2001077350A3
WO2001077350A3 PCT/US2001/011436 US0111436W WO0177350A3 WO 2001077350 A3 WO2001077350 A3 WO 2001077350A3 US 0111436 W US0111436 W US 0111436W WO 0177350 A3 WO0177350 A3 WO 0177350A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
present
eukaryotic cell
endogenous gene
compositions
Prior art date
Application number
PCT/US2001/011436
Other languages
French (fr)
Other versions
WO2001077350A2 (en
Inventor
Kenneth E Palmer
Gregory P Pogue
Original Assignee
Large Scale Biology Corp
Kenneth E Palmer
Gregory P Pogue
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Large Scale Biology Corp, Kenneth E Palmer, Gregory P Pogue filed Critical Large Scale Biology Corp
Priority to AU2001253255A priority Critical patent/AU2001253255A1/en
Publication of WO2001077350A2 publication Critical patent/WO2001077350A2/en
Publication of WO2001077350A3 publication Critical patent/WO2001077350A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides an eukaryotic recombinant vector suited for bi-directional transcription of a transgene to yield both sense and antisense RNA transcripts of the transgene in an eukaryotic cell. The invention vectors are particularly suited for mediating gene silencing in a variety of biological systems. The present invention also provides host cells and transgenic plants comprising the invention vectors. Further provided by the invention are methods of inhibiting expression of an endogenous gene present in an eukaryotic cell. Also included is a method of identifying a biological function(s) of an endogenous gene of interest in an eukaryotic cell by selectively inhibiting the expression of the endogenous gene.
PCT/US2001/011436 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression WO2001077350A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001253255A AU2001253255A1 (en) 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54557400A 2000-04-07 2000-04-07
US09/545,574 2000-04-07

Publications (2)

Publication Number Publication Date
WO2001077350A2 WO2001077350A2 (en) 2001-10-18
WO2001077350A3 true WO2001077350A3 (en) 2002-08-08

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/011436 WO2001077350A2 (en) 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression

Country Status (2)

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AU (1) AU2001253255A1 (en)
WO (1) WO2001077350A2 (en)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
CA2427347C (en) * 2000-10-31 2011-01-18 Commonwealth Scientific And Industrial Research Organisation Method and means for producing barley yellow dwarf virus resistant cereal plants
JP2005527639A (en) 2001-11-02 2005-09-15 インサート セラピューティクス インコーポレイテッド Methods and compositions for therapeutic use of RNA interference
AU2003283951A1 (en) 2002-05-23 2004-03-03 Ceptyr, Inc. Modulation of biological signal transduction by rna interference
US7148342B2 (en) 2002-07-24 2006-12-12 The Trustees Of The University Of Pennyslvania Compositions and methods for sirna inhibition of angiogenesis
AU2002951347A0 (en) * 2002-09-09 2002-09-26 Benitec Australia Ltd Genetic silencing
AU2004229399B2 (en) 2003-04-07 2010-08-05 Prospect Therapeutics, Inc. Composition and uses of galectin antagonists
JP5184077B2 (en) 2004-03-26 2013-04-17 キュリス，インコーポレイテッド RNA interference modulator of hedgehog signaling and use thereof
DE602005015302D1 (en) 2004-04-05 2009-08-20 Univ California Nkg2d modulation
JP2008506703A (en) 2004-07-14 2008-03-06 ユニバーシティ オブ ユタ リサーチ ファウンデーション Netrin-related compounds and uses
US20060063208A1 (en) 2004-08-02 2006-03-23 Woolf Clifford J DRG11-responsive (DRAGON) gene and uses thereof
US7517870B2 (en) 2004-12-03 2009-04-14 Fondazione Telethon Use of compounds that interfere with the hedgehog signaling pathway for the manufacture of a medicament for preventing, inhibiting, and/or reversing ocular diseases related with ocular neovascularization
DK1866414T3 (en) 2005-03-31 2012-04-23 Calando Pharmaceuticals Inc Inhibitors of ribonucleotide reductase subunit 2 and uses thereof.
US8715665B2 (en) 2007-04-13 2014-05-06 The General Hospital Corporation Methods for treating cancer resistant to ErbB therapeutics
EP2164875A4 (en) 2007-05-24 2011-11-30 Us Dept Veterans Affairs Intranuclear protein transduction through a nucleoside salvage pathway
BRPI0911332A2 (en) 2008-04-04 2019-09-24 Calando Pharmaceuticals Inc compositions and use of epas1 inhibitors
SG171952A1 (en) 2008-12-04 2011-07-28 Opko Ophthalmics Llc Compositions and methods for selective inhibition of pro-angiogenic vegf isoforms
IT1398977B1 (en) 2009-06-25 2013-03-28 Icgeb ANTI-PANT AGENT SPECIFIC FOR CEREBRAL CANCERS WITH RECQ1 SUPPRESSION MECHANISM
GB201103167D0 (en) 2011-02-24 2011-04-06 Isis Innovation Gene silencing
CA2885544A1 (en) 2012-09-28 2014-04-03 The University Of North Carolina At Chapel Hill Aav vectors targeted to oligodendrocytes
CA3000633C (en) 2014-10-14 2023-10-03 The Regents Of The University Of California Use of cdk9 and brd4 inhibitors to inhibit inflammation
EP4344741A2 (en) 2014-11-21 2024-04-03 The University of North Carolina at Chapel Hill Aav vectors targeted to the central nervous system
CN107531774B (en) 2015-02-06 2021-12-14 北卡罗来纳大学查珀尔希尔分校 Optimized human coagulation factor VIII gene expression cassette and application thereof
CA2978449A1 (en) 2015-03-02 2016-09-09 180 Therapeutics Lp Method of treating a localized fibrotic disorder using an il-33 antagonist
US11344511B2 (en) 2016-07-27 2022-05-31 Case Western Reserve University Compounds and methods of promoting myelination
JP7416406B2 (en) 2016-12-08 2024-01-17 ケース ウェスタン リザーブ ユニバーシティ Methods and compositions for increasing functional myelin production
KR20210130158A (en) * 2019-01-31 2021-10-29 오레곤 헬스 앤드 사이언스 유니버시티 Methods Using Transcription-Dependent Directed Evolution of AAV Capsids

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ROGERS S A ET AL: "ANALYSIS OF CHLOROPLAST PROMOTERS USING BIDIRECTIONAL TRANSCRIPTION VECTORS", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 15, no. 3, 1990, pages 421 - 435, XP002159238, ISSN: 0167-4412 *
SPICER D B ET AL: "AN ANTISENSE PROMOTER OF THE MURINE C-MYC GENE IS LOCALIZED WITHIN INTRON 2", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 12, no. 3, March 1992 (1992-03-01), pages 1324 - 1329, XP002910389, ISSN: 0270-7306 *
SUREAU ALAIN ET AL: "Characterization of multiple alternative RNAs resulting from antisense transcription of the PR264/SC35 splicing factor gene.", NUCLEIC ACIDS RESEARCH, vol. 25, no. 22, 15 November 1997 (1997-11-15), pages 4513 - 4522, XP002197926, ISSN: 0305-1048 *
VELHAGEN IRIS ET AL: "An antisense promoter within the hepatitis B virus X gene.", INTERVIROLOGY, vol. 38, no. 3-4, 1995, pages 127 - 133, XP008002810, ISSN: 0300-5526 *

Also Published As

Publication number Publication date
WO2001077350A2 (en) 2001-10-18
AU2001253255A1 (en) 2001-10-23

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