WO2001069256A3 - Sensorelement zur optischen detektion von chemischen oder biochemischen analyten - Google Patents

Sensorelement zur optischen detektion von chemischen oder biochemischen analyten Download PDF

Info

Publication number
WO2001069256A3
WO2001069256A3 PCT/DE2001/000672 DE0100672W WO0169256A3 WO 2001069256 A3 WO2001069256 A3 WO 2001069256A3 DE 0100672 W DE0100672 W DE 0100672W WO 0169256 A3 WO0169256 A3 WO 0169256A3
Authority
WO
WIPO (PCT)
Prior art keywords
sensor element
outer layer
samples
optically detecting
detecting chemical
Prior art date
Application number
PCT/DE2001/000672
Other languages
English (en)
French (fr)
Other versions
WO2001069256A2 (de
Inventor
Ralf Waldhaeusl
Norbert Danz
Original Assignee
Fraunhofer Ges Forschung
Ralf Waldhaeusl
Norbert Danz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Ges Forschung, Ralf Waldhaeusl, Norbert Danz filed Critical Fraunhofer Ges Forschung
Priority to EP01916889A priority Critical patent/EP1264180A2/de
Priority to JP2001568084A priority patent/JP2003531361A/ja
Priority to AU44071/01A priority patent/AU4407101A/en
Priority to US10/221,588 priority patent/US20030132406A1/en
Publication of WO2001069256A2 publication Critical patent/WO2001069256A2/de
Publication of WO2001069256A3 publication Critical patent/WO2001069256A3/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Die Erfindung betrifft ein Sensorelement zur optischen Detektion von chemischen oder biochemischen Analyten, die in unterschiedlichen Proben enthalten sein können. Aufgabengemäß sollen ein möglichst kleines Probenvolumen erforderlich und die einzelnen Proben relativ dicht benachbart zueinander angeordnet werden können, und trotzdem eine hohe Messgenauigkeit erreicht werden. Am Sensorelement ist mindestens eine Grenzfläche ausgebildet, an der infolge von Totalreflexion ein evaneszentes Feld ausgebildet wird. Die Proben sind in voneinander getrennten Kavitäten aufgenommen und die Kavitäten innerhalb einer unmittelbar auf einem Substrat aufgebrachten strukturierten Deckschicht ausgebildet. Dabei ist die Schichtdicke der Deckschicht zumindest größer als die Eindringtiefe des evaneszenten Feldes. Die Deckschicht besteht aus einem fluorierten Polymer und verhindert einen Stoffaustausch der einzelnen Proben, die in den verschiedenen Kavitäten aufgenommen sind.
PCT/DE2001/000672 2000-03-13 2001-02-16 Sensorelement zur optischen detektion von chemischen oder biochemischen analyten WO2001069256A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP01916889A EP1264180A2 (de) 2000-03-13 2001-02-16 Sensorelement zur optischen detektion von chemischen oder biochemischen analyten
JP2001568084A JP2003531361A (ja) 2000-03-13 2001-02-16 センサーエレメントおよびその製造方法並びに検体検出方法
AU44071/01A AU4407101A (en) 2000-03-13 2001-02-16 Sensor element for optically detecting chemical or biochemical analytes
US10/221,588 US20030132406A1 (en) 2000-03-13 2001-02-16 Sensor element for optically detecting chemical or biochemical analytes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10012793.2 2000-03-13
DE10012793A DE10012793C2 (de) 2000-03-13 2000-03-13 Sensorelement zur optischen Detektion von chemischen oder biochemischen Analyten

Publications (2)

Publication Number Publication Date
WO2001069256A2 WO2001069256A2 (de) 2001-09-20
WO2001069256A3 true WO2001069256A3 (de) 2002-04-11

Family

ID=7634935

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/000672 WO2001069256A2 (de) 2000-03-13 2001-02-16 Sensorelement zur optischen detektion von chemischen oder biochemischen analyten

Country Status (6)

Country Link
US (1) US20030132406A1 (de)
EP (1) EP1264180A2 (de)
JP (1) JP2003531361A (de)
AU (1) AU4407101A (de)
DE (1) DE10012793C2 (de)
WO (1) WO2001069256A2 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002139418A (ja) * 2000-11-01 2002-05-17 Nikon Corp マイクロウエルプレート及びマイクロウエルプレートを備える蛍光検出装置
JP3668198B2 (ja) * 2002-02-18 2005-07-06 株式会社東芝 光導波路型マイクロプレート
US20050084909A1 (en) * 2003-08-29 2005-04-21 Kenichi Uchiyama Antigen measuring device and method thereof, an antibody chip package and a pallet
WO2005052557A1 (en) * 2003-11-28 2005-06-09 Lumiscence A/S An examination system for examination of a specimen; sub-units and units therefore, a sensor and a microscope
DE102004027957A1 (de) * 2004-06-08 2005-12-29 Carl Zeiss Jena Gmbh Verfahren und Anordnung zur Untersuchung der Wechselwirkung von Biomolekülen
TWI247886B (en) * 2005-01-06 2006-01-21 Univ Nat Taiwan Improved linear wave-guide type surface plasmon resonance micro sensor
US8349605B1 (en) 2005-04-12 2013-01-08 Colorado State University Research Foundation Optical analyte sensor
EP3936857B1 (de) * 2006-09-01 2023-06-21 Pacific Biosciences Of California, Inc. Substrate, systeme und verfahren zur analyse von materialien
JP4883398B2 (ja) * 2006-09-06 2012-02-22 独立行政法人産業技術総合研究所 エバネッセント波励起蛍光観察における背景光低減方法及び部材
SE531493C2 (sv) * 2006-10-31 2009-04-28 Knut Johansen Sensor
JP4597175B2 (ja) * 2007-09-21 2010-12-15 株式会社日立ハイテクノロジーズ 標的物質を検出するための分析装置、又は分析方法、若しくはこれら分析装置及び分析方法に用いるデバイス
US20100252751A1 (en) * 2007-11-05 2010-10-07 Koninklijke Philips Electronics N.V. Microelectronic opiacal evanescent field sensor
JP2010066212A (ja) * 2008-09-12 2010-03-25 Univ Of Tokyo 測定装置及び観察対象の測定方法
EP3943920B1 (de) * 2010-02-19 2024-04-03 Pacific Biosciences Of California, Inc. Integriertes analytisches system und verfahren zur fluoreszenzmessung
US8994946B2 (en) 2010-02-19 2015-03-31 Pacific Biosciences Of California, Inc. Integrated analytical system and method
US8790591B2 (en) * 2010-07-09 2014-07-29 Case Western Reserve University In vitro point-of-care sensor and method of use
US9372308B1 (en) 2012-06-17 2016-06-21 Pacific Biosciences Of California, Inc. Arrays of integrated analytical devices and methods for production
JP6029899B2 (ja) * 2012-09-07 2016-11-24 日東電工株式会社 Ｓｐｒセンサセルおよびｓｐｒセンサ
EP3524964B1 (de) 2012-12-18 2020-07-15 Pacific Biosciences Of California, Inc. Optische analysevorrichtung
US20140311925A1 (en) * 2013-01-09 2014-10-23 Case Western Reserve University In vitro point-of-care sensor and method of use
US9624540B2 (en) 2013-02-22 2017-04-18 Pacific Biosciences Of California, Inc. Integrated illumination of optical analytical devices
EP3186617A4 (de) 2014-08-27 2018-04-25 Pacific Biosciences Of California, Inc. Anordnungen aus integrierten analytischen vorrichtungen
US10487356B2 (en) 2015-03-16 2019-11-26 Pacific Biosciences Of California, Inc. Integrated devices and systems for free-space optical coupling
CA2983882C (en) 2015-05-07 2023-10-03 Scott E. Helgesen Multiprocessor pipeline architecture
CA2989344C (en) 2015-06-12 2023-09-26 Pacific Biosciences Of California, Inc. Integrated target waveguide devices and systems for optical coupling

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994027137A2 (en) * 1993-05-18 1994-11-24 University Of Utah Research Foundation Apparatus and methods for multianalyte homogeneous fluoroimmunoassays
WO1995003538A1 (en) * 1993-07-20 1995-02-02 Balzers Aktiengesellschaft Optical biosensor matrix
WO1996035940A1 (en) * 1995-05-12 1996-11-14 Novartis Ag Sensor platform and method for the parallel detection of a plurality of analytes using evanescently excited luminescence
US5639671A (en) * 1989-09-18 1997-06-17 Biostar, Inc. Methods for optimizing of an optical assay device
WO1998021571A1 (en) * 1996-11-11 1998-05-22 Novartis Ag Use of biosensors to diagnose plant diseases
WO1999054736A1 (en) * 1998-04-21 1999-10-28 Beckman Coulter, Inc. Detection of very low quantities of analyte
EP0971226A1 (de) * 1998-07-06 2000-01-12 Suzuki Motor Corporation SPR Sensorzelle und diese verwendende Vorrichtung für Immunoassay

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4913519A (en) * 1988-03-04 1990-04-03 Fiberchem Inc. Optical sensor for the detection of ice formation and other chemical species
GB8811919D0 (en) * 1988-05-20 1988-06-22 Amersham Int Plc Biological sensors
US5919712A (en) * 1993-05-18 1999-07-06 University Of Utah Research Foundation Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
DE19732619C2 (de) * 1997-07-29 1999-08-19 Fraunhofer Ges Forschung Optische Detektoreinrichtung
NL1008934C2 (nl) * 1998-04-20 1999-10-21 Univ Twente Geïntegreerde optische lichtgeleider inrichting.

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639671A (en) * 1989-09-18 1997-06-17 Biostar, Inc. Methods for optimizing of an optical assay device
WO1994027137A2 (en) * 1993-05-18 1994-11-24 University Of Utah Research Foundation Apparatus and methods for multianalyte homogeneous fluoroimmunoassays
WO1995003538A1 (en) * 1993-07-20 1995-02-02 Balzers Aktiengesellschaft Optical biosensor matrix
WO1996035940A1 (en) * 1995-05-12 1996-11-14 Novartis Ag Sensor platform and method for the parallel detection of a plurality of analytes using evanescently excited luminescence
WO1998021571A1 (en) * 1996-11-11 1998-05-22 Novartis Ag Use of biosensors to diagnose plant diseases
WO1999054736A1 (en) * 1998-04-21 1999-10-28 Beckman Coulter, Inc. Detection of very low quantities of analyte
EP0971226A1 (de) * 1998-07-06 2000-01-12 Suzuki Motor Corporation SPR Sensorzelle und diese verwendende Vorrichtung für Immunoassay

Also Published As

Publication number Publication date
DE10012793C2 (de) 2002-01-24
JP2003531361A (ja) 2003-10-21
AU4407101A (en) 2001-09-24
EP1264180A2 (de) 2002-12-11
DE10012793A1 (de) 2001-09-27
WO2001069256A2 (de) 2001-09-20
US20030132406A1 (en) 2003-07-17

Similar Documents

Publication Publication Date Title
WO2001069256A3 (de) Sensorelement zur optischen detektion von chemischen oder biochemischen analyten
EP1262768A4 (de) Analytisches element, messgerät und verfahren zur identifizierung eines substrates mit diesem element
EP1667581A1 (de) Probenvorrichtung mit kapillarwirkung
WO2001055691A3 (de) Wellenleiterplatte sowie darauf basierende sensorplattformen, anordnungen von probenbehältnissen und nachweisverfahren
Henares et al. Integration of multianalyte sensing functions on a capillary-assembled microchip: Simultaneous determination of ion concentrations and enzymatic activities by a “drop-and-sip” technique
WO1999064620A3 (en) Diagnostic assay requiring a small sample of biological fluid
WO2006072043A3 (en) Label-independent detection of unpurified analytes
WO2005069737A3 (en) Method and system for detecting analytes
WO2008135564A3 (en) Assays
CA2479452A1 (en) Method and device for determining analytes in a liquid
AU2003298412A1 (en) Analytical platform and identification method
WO2004106891A3 (en) Ultrasensitive biochemical sensor
WO2001092870A3 (de) Kit und verfahren zur multianalytbestimmung
EP1087223A4 (de) Analysegerät
WO2008051742A3 (en) Transient decay amperometry
EP0945721A3 (de) Biosensorelement für Oberflächen-Plasmon-Resonanz-Messungen und dessen Herstellungsverfahren
AU2005202517A1 (en) Methods for modulation of flow in a flow pathway
WO2008062048A3 (en) Methods for optically detecting multiple analytes in a liquid sample with a compressible microfluidic device
WO2005073405A3 (en) Catalytic polynucleotide and its use for determination of analytes
WO2007023372A3 (fr) Dosage d'un analyte par immunochromatographie avec migration laterale
WO2004102170A8 (fr) Biopuce a reseau de fibres optiques basee sur la spectroscopie a interferences par reflectometrie en lumiere blanche
WO2006044957A3 (en) Porous photonic crystal with light scattering domains and methods of synthesis and use thereof
WO2003014697A3 (en) Amplification of analyte detection with passivated enhancing surfaces having receptors
US20040248306A1 (en) Microfluidic water analytical device
WO2005038419A8 (en) A method and device for detecting small numbers of molecules using surface-enhanced coherent anti-stokes raman spectroscopy

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DK DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DK DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2001916889

Country of ref document: EP

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 568084

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 10221588

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2001916889

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2001916889

Country of ref document: EP