WO2001038511A8 - Resolvase-catalysed, sequence-specific dna-recombination in eukaryotic cells - Google Patents

Resolvase-catalysed, sequence-specific dna-recombination in eukaryotic cells

Info

Publication number
WO2001038511A8
WO2001038511A8 PCT/EP2000/011718 EP0011718W WO0138511A8 WO 2001038511 A8 WO2001038511 A8 WO 2001038511A8 EP 0011718 W EP0011718 W EP 0011718W WO 0138511 A8 WO0138511 A8 WO 0138511A8
Authority
WO
WIPO (PCT)
Prior art keywords
sequence
resolvase
introducing
eukaryotic cells
recombination
Prior art date
Application number
PCT/EP2000/011718
Other languages
German (de)
French (fr)
Other versions
WO2001038511A2 (en
WO2001038511A3 (en
Inventor
Peter Droege
Original Assignee
Cardion Ag
Peter Droege
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cardion Ag, Peter Droege filed Critical Cardion Ag
Priority to CA002394827A priority Critical patent/CA2394827A1/en
Priority to AU18239/01A priority patent/AU1823901A/en
Priority to JP2001540261A priority patent/JP2003533176A/en
Priority to EP00977566A priority patent/EP1240316A2/en
Publication of WO2001038511A2 publication Critical patent/WO2001038511A2/en
Publication of WO2001038511A3 publication Critical patent/WO2001038511A3/en
Publication of WO2001038511A8 publication Critical patent/WO2001038511A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Abstract

The invention relates to a method for the sequence-specific recombination of DNA in eukaryotic cells, comprising the following steps: introducing a first DNA sequence which contains a res sequence into a cell, introducing a copy of the first DNA sequence and performing the sequence-specific recombination using the influence of a resolvase or its derivative. The invention also relates to a method for testing topoisomerase inhibitors in a eukaryotic cell, comprising the following steps: introducing a first DNA sequence according to SEQ ID NO:1 into a cell, introducing a copy of the first DNA sequence according to SEQ ID NO:1 in direct orientation, introducing a wild-type resolvase and adding the topoisomerase inhibitors which are to be tested.
PCT/EP2000/011718 1999-11-24 2000-11-24 Resolvase-catalysed, sequence-specific dna-recombination in eukaryotic cells WO2001038511A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002394827A CA2394827A1 (en) 1999-11-24 2000-11-24 Resolvase-catalysed, sequence-specific dna-recombination in eukaryotic cells
AU18239/01A AU1823901A (en) 1999-11-24 2000-11-24 Resolvase-catalysed, sequence-specific dna-recombination in eukaryotic cells
JP2001540261A JP2003533176A (en) 1999-11-24 2000-11-24 Resolvese-catalyzed sequence-specific recombination of DNA in eukaryotic cells
EP00977566A EP1240316A2 (en) 1999-11-24 2000-11-24 Resolvase-catalysed, sequence-specific dna-recombination in eukaryotic cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19956513.9 1999-11-24
DE1999156513 DE19956513A1 (en) 1999-11-24 1999-11-24 Resolvase-catalyzed, sequence-specific DNA recombination in eukaryotic cells

Publications (3)

Publication Number Publication Date
WO2001038511A2 WO2001038511A2 (en) 2001-05-31
WO2001038511A3 WO2001038511A3 (en) 2002-05-10
WO2001038511A8 true WO2001038511A8 (en) 2002-05-30

Family

ID=7930159

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/011718 WO2001038511A2 (en) 1999-11-24 2000-11-24 Resolvase-catalysed, sequence-specific dna-recombination in eukaryotic cells

Country Status (6)

Country Link
EP (1) EP1240316A2 (en)
JP (1) JP2003533176A (en)
AU (1) AU1823901A (en)
CA (1) CA2394827A1 (en)
DE (1) DE19956513A1 (en)
WO (1) WO2001038511A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2739738B1 (en) 2011-08-03 2018-06-20 Ramot at Tel Aviv University, Ltd. Use of integrase for targeted gene expression

Also Published As

Publication number Publication date
AU1823901A (en) 2001-06-04
CA2394827A1 (en) 2001-05-31
WO2001038511A2 (en) 2001-05-31
DE19956513A1 (en) 2001-06-21
EP1240316A2 (en) 2002-09-18
JP2003533176A (en) 2003-11-11
WO2001038511A3 (en) 2002-05-10

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