WO2001029248A3 - Method for amplifying and detecting nucleic acid - Google Patents

Method for amplifying and detecting nucleic acid Download PDF

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Publication number
WO2001029248A3
WO2001029248A3 PCT/KR1999/000628 KR9900628W WO0129248A3 WO 2001029248 A3 WO2001029248 A3 WO 2001029248A3 KR 9900628 W KR9900628 W KR 9900628W WO 0129248 A3 WO0129248 A3 WO 0129248A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotide primers
nucleic acid
present
amplifying
solid support
Prior art date
Application number
PCT/KR1999/000628
Other languages
French (fr)
Other versions
WO2001029248A2 (en
WO2001029248B1 (en
Inventor
Gi Young Jang
Original Assignee
Bionex Inc
Gi Young Jang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bionex Inc, Gi Young Jang filed Critical Bionex Inc
Priority to PCT/KR1999/000628 priority Critical patent/WO2001029248A2/en
Publication of WO2001029248A2 publication Critical patent/WO2001029248A2/en
Publication of WO2001029248A3 publication Critical patent/WO2001029248A3/en
Publication of WO2001029248B1 publication Critical patent/WO2001029248B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

The present invention provides a method for amplifying and detecting target nucleic acid. One method of present invention comprises Polymerase Chain Reaction (PCR) amplification process with two different standard oligonucleotide primers along with extra number of oligonucleotide primers bound to a solid support. The extra number of oligonucleotide primers bound to the solid support can be co-amplified along with equal or preferably unequal amount of two standard oligonucleotide primers. Another method of present invention involves multi-number of target amplification by periodical addition of the oligonucleotide primers during the amplification process. These processes are useful for specifying the amplified nucleic acid, which allow the simple and convenient format for diagnostic and DNA sequencing.
PCT/KR1999/000628 1999-10-19 1999-10-19 Method for amplifying and detecting nucleic acid WO2001029248A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/KR1999/000628 WO2001029248A2 (en) 1999-10-19 1999-10-19 Method for amplifying and detecting nucleic acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR1999/000628 WO2001029248A2 (en) 1999-10-19 1999-10-19 Method for amplifying and detecting nucleic acid

Publications (3)

Publication Number Publication Date
WO2001029248A2 WO2001029248A2 (en) 2001-04-26
WO2001029248A3 true WO2001029248A3 (en) 2001-09-20
WO2001029248B1 WO2001029248B1 (en) 2001-12-06

Family

ID=19571119

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR1999/000628 WO2001029248A2 (en) 1999-10-19 1999-10-19 Method for amplifying and detecting nucleic acid

Country Status (1)

Country Link
WO (1) WO2001029248A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005029810B4 (en) * 2005-06-27 2008-11-13 Siemens Ag Method for detecting nucleotide sequences, use of the method and test set

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990011369A1 (en) * 1989-03-22 1990-10-04 Cemu Bioteknik Ab Solid phase diagnosis of medical conditions
WO1995006753A1 (en) * 1993-09-03 1995-03-09 The United States of America, represented by The Secretary, Department of Health & Human Services Method and compositions for primer specific solid-phase detection of pcr products
WO1995015400A1 (en) * 1993-12-03 1995-06-08 The Johns Hopkins University Genotyping by simultaneous analysis of multiple microsatellite loci
WO1996036735A1 (en) * 1995-05-19 1996-11-21 American Type Culture Collection A mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers
EP0787205A1 (en) * 1994-10-28 1997-08-06 Genset Sa Solid-phase nucleic acid amplification method and reagent kit therefor
WO1999047701A1 (en) * 1998-03-18 1999-09-23 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Method for detecting a nucleotide sequence

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990011369A1 (en) * 1989-03-22 1990-10-04 Cemu Bioteknik Ab Solid phase diagnosis of medical conditions
WO1995006753A1 (en) * 1993-09-03 1995-03-09 The United States of America, represented by The Secretary, Department of Health & Human Services Method and compositions for primer specific solid-phase detection of pcr products
WO1995015400A1 (en) * 1993-12-03 1995-06-08 The Johns Hopkins University Genotyping by simultaneous analysis of multiple microsatellite loci
EP0787205A1 (en) * 1994-10-28 1997-08-06 Genset Sa Solid-phase nucleic acid amplification method and reagent kit therefor
WO1996036735A1 (en) * 1995-05-19 1996-11-21 American Type Culture Collection A mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers
WO1999047701A1 (en) * 1998-03-18 1999-09-23 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Method for detecting a nucleotide sequence

Also Published As

Publication number Publication date
WO2001029248A2 (en) 2001-04-26
WO2001029248B1 (en) 2001-12-06

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