WO2001020008A3 - Vascular-specific promoters - Google Patents

Vascular-specific promoters Download PDF

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Publication number
WO2001020008A3
WO2001020008A3 PCT/EP2000/006248 EP0006248W WO0120008A3 WO 2001020008 A3 WO2001020008 A3 WO 2001020008A3 EP 0006248 W EP0006248 W EP 0006248W WO 0120008 A3 WO0120008 A3 WO 0120008A3
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WO
WIPO (PCT)
Prior art keywords
vascular
specific promoters
dna fragment
variants
portions
Prior art date
Application number
PCT/EP2000/006248
Other languages
French (fr)
Other versions
WO2001020008A2 (en
Inventor
J Harry C Di Hoge
Enrico Scarpella
Anneke Hf Hoge-Meppelink
Annemarie H Meijer
Original Assignee
Univ Leiden
Enrico Scarpella
Hoge Meppelink Anneke Hf
Annemarie H Meijer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leiden, Enrico Scarpella, Hoge Meppelink Anneke Hf, Annemarie H Meijer filed Critical Univ Leiden
Priority to AU58244/00A priority Critical patent/AU5824400A/en
Publication of WO2001020008A2 publication Critical patent/WO2001020008A2/en
Publication of WO2001020008A3 publication Critical patent/WO2001020008A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Communicable Diseases (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A DNA fragment upstream of the rice Oshox1 gene or portions or variants of the DNA fragment wherein the DNA fragment or portions or variants thereof are capable of promoting vascular expression of an associated DNA sequence upon reintroduction into a plant, typically wherein vascular expression of the associated DNA sequence enables production of an antipathogenic protein.
PCT/EP2000/006248 1999-09-16 2000-07-03 Vascular-specific promoters WO2001020008A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU58244/00A AU5824400A (en) 1999-09-16 2000-07-03 Vascular-specific promoters

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99203030 1999-09-16
EP99203030.4 1999-09-16

Publications (2)

Publication Number Publication Date
WO2001020008A2 WO2001020008A2 (en) 2001-03-22
WO2001020008A3 true WO2001020008A3 (en) 2001-08-09

Family

ID=8240647

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/006248 WO2001020008A2 (en) 1999-09-16 2000-07-03 Vascular-specific promoters

Country Status (2)

Country Link
AU (1) AU5824400A (en)
WO (1) WO2001020008A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7365186B2 (en) * 2002-11-22 2008-04-29 Arborgen, Llc Vascular-preferred promoter sequences and uses thereof
JP4312012B2 (en) * 2003-09-12 2009-08-12 トヨタ自動車株式会社 Paraquat® resistance gene and vascular and trichome specific promoters
FR2870257A1 (en) * 2004-05-14 2005-11-18 Genoplante Valor Soc Par Actio PROMOTERS INDUCIBLE BY PEST INSECTS, AND USES THEREOF
FR2881143B1 (en) 2005-01-27 2010-12-17 Librophyt SYSTEM FOR PRODUCING TERPENOIDS IN PLANTS
FR2914652B1 (en) 2007-04-03 2013-01-18 Librophyt GENES ENCODING Z, Z-FARNESYL DIPHOSPHATE SYNTHASE AND MULTI-PRODUCT SESQUITERPENE SYNTHASE AND USES THEREOF
EP2055783A1 (en) * 2007-10-30 2009-05-06 VIB vzw Insect inhibiting plant serpin mutants
CN101818170B (en) * 2009-07-17 2014-07-16 西南大学 Plant expression vector for vascular peculiar promoter to control antimicrobial protein gene and method for cultivating greensickness-resistant cotton
KR101322319B1 (en) 2012-05-14 2013-10-28 대한민국 A method increasing tiller numbers of plants using hox10 gene

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990005187A1 (en) * 1988-11-07 1990-05-17 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Wound-induced dna sequence from solanum tuberosum and its use
WO1996032007A1 (en) * 1995-04-13 1996-10-17 Yeda Research And Development Co. Ltd. Transgenic tomato plants containing a fusarium resitance gene
WO1999024561A2 (en) * 1997-11-12 1999-05-20 Board Of Control Of Michigan Technological University Genetic engineering of lignin biosynthesis in plants

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990005187A1 (en) * 1988-11-07 1990-05-17 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Wound-induced dna sequence from solanum tuberosum and its use
WO1996032007A1 (en) * 1995-04-13 1996-10-17 Yeda Research And Development Co. Ltd. Transgenic tomato plants containing a fusarium resitance gene
WO1999024561A2 (en) * 1997-11-12 1999-05-20 Board Of Control Of Michigan Technological University Genetic engineering of lignin biosynthesis in plants

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE EMPLN EMBL Heidelberg, Germany; 26 December 1999 (1999-12-26), SCARPELLA E ET AL.: "Characterization of the Oshox1 gene expression pattern during rice development", XP002163190 *
MEIJER A H ET AL: "Transcriptional repression by Oshox1, a novel homeodomain leucine zipper protein from rice.", PLANT JOURNAL, vol. 11, no. 2, February 1997 (1997-02-01), pages 263 - 276, XP002130665 *
RAO K V ET AL: "Expression of snowdrop lectin (GNA) in transgenic rice plants confers resistance to rice brown planthopper.", PLANT JOURNAL, vol. 15, no. 4, August 1998 (1998-08-01), pages 469 - 477, XP002163436, ISSN: 0960-7412 *
TORNERO P ET AL.: "Phloem-specific expression of a plant homeobox gene during secondary phases of vascular development", PLANT JOURNAL, vol. 9, no. 5, May 1996 (1996-05-01), pages 639 - 648, XP002130666 *

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Publication number Publication date
AU5824400A (en) 2001-04-17
WO2001020008A2 (en) 2001-03-22

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