WO2001011025A3 - Crystallization and structure determination of staphylococcus aureus thymidylate kinase - Google Patents

Crystallization and structure determination of staphylococcus aureus thymidylate kinase Download PDF

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Publication number
WO2001011025A3
WO2001011025A3 PCT/US2000/021425 US0021425W WO0111025A3 WO 2001011025 A3 WO2001011025 A3 WO 2001011025A3 US 0021425 W US0021425 W US 0021425W WO 0111025 A3 WO0111025 A3 WO 0111025A3
Authority
WO
WIPO (PCT)
Prior art keywords
staphylococcus aureus
crystallization
thymidylate kinase
structure determination
aureus thymidylate
Prior art date
Application number
PCT/US2000/021425
Other languages
French (fr)
Other versions
WO2001011025A2 (en
Inventor
Timothy E Benson
Original Assignee
Upjohn Co
Timothy E Benson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Upjohn Co, Timothy E Benson filed Critical Upjohn Co
Priority to EP00953850A priority Critical patent/EP1200565A2/en
Priority to AU66227/00A priority patent/AU781654B2/en
Priority to CA002378010A priority patent/CA2378010A1/en
Priority to JP2001515812A priority patent/JP2003517818A/en
Publication of WO2001011025A2 publication Critical patent/WO2001011025A2/en
Publication of WO2001011025A3 publication Critical patent/WO2001011025A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1229—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Abstract

An unliganded form of Staphylococcus aureus thymidylate kinase (S. aureus TMK) has been crystallized, and the three dimensional x-ray crystal structure has been solved to 2.3 Å resolution. The x-ray crystal structure is useful for solving the structure of other molecules or molecular complexes, and designing inhibitors of S. aureus TMK activity.
PCT/US2000/021425 1999-08-04 2000-08-04 Crystallization and structure determination of staphylococcus aureus thymidylate kinase WO2001011025A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00953850A EP1200565A2 (en) 1999-08-04 2000-08-04 Crystallization and structure determination of staphylococcus aureus thymidylate kinase
AU66227/00A AU781654B2 (en) 1999-08-04 2000-08-04 Crystallization and structure determination of staphylococcus aureus thymidylate kinase
CA002378010A CA2378010A1 (en) 1999-08-04 2000-08-04 Crystallization and structure determination of staphylococcus aureus thymidylate kinase
JP2001515812A JP2003517818A (en) 1999-08-04 2000-08-04 Crystallization and structure determination of Staphylococcus aureus thymidylate kinase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14711799P 1999-08-04 1999-08-04
US60/147,117 1999-08-04

Publications (2)

Publication Number Publication Date
WO2001011025A2 WO2001011025A2 (en) 2001-02-15
WO2001011025A3 true WO2001011025A3 (en) 2001-08-23

Family

ID=22520348

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/021425 WO2001011025A2 (en) 1999-08-04 2000-08-04 Crystallization and structure determination of staphylococcus aureus thymidylate kinase

Country Status (5)

Country Link
EP (1) EP1200565A2 (en)
JP (1) JP2003517818A (en)
AU (1) AU781654B2 (en)
CA (1) CA2378010A1 (en)
WO (1) WO2001011025A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1624849A2 (en) * 2003-05-07 2006-02-15 Smithkline Beecham Corporation Methionine aminopeptidase and methods of use
US8741614B2 (en) * 2010-11-02 2014-06-03 Probiodrug Ag Crystal structure of isoglutaminyl cyclase

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0786519A2 (en) * 1996-01-05 1997-07-30 Human Genome Sciences, Inc. Staphylococcus aureus polynucleotides and sequences
WO2000012678A2 (en) * 1998-09-01 2000-03-09 Human Genome Sciences, Inc. Staphylococcus aureus genes and polypeptides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0786519A2 (en) * 1996-01-05 1997-07-30 Human Genome Sciences, Inc. Staphylococcus aureus polynucleotides and sequences
WO2000012678A2 (en) * 1998-09-01 2000-03-09 Human Genome Sciences, Inc. Staphylococcus aureus genes and polypeptides

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
A LAVIE ET AL: "Crystal structure of yeast thymidylate kinase complexed with the bisubstrate inhibitor TP5A at 2.0 A resolution: implications for catalysis and AZT activation", BIOCHEMISTRY,AMERICAN CHEMICAL SOCIETY. EASTON, PA,US, vol. 37, 1998, pages 3677 - 3686, XP002111244, ISSN: 0006-2960 *
A LAVIE ET AL: "Structural basis for the efficient phosphorylation of 3'-azidothymidine monophosphate by E. coli thymidylate kinase", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,NATIONAL ACADEMY OF SCIENCE. WASHINGTON,US, vol. 95, November 1998 (1998-11-01), pages 14045 - 14050, XP002111243, ISSN: 0027-8424 *
DE LA SIERRA I LI ET AL: "Crystallization and preliminary X-ray analysis of the thymidylate kinase from Mycobacterium tuberculosis.", ACTA CRYSTALLOGRAPHICA SECTION D BIOLOGICAL CRYSTALLOGRAPHY, vol. 56, no. 2, February 2000 (2000-02-01), pages 226 - 228, XP000982605, ISSN: 0907-4449 *
OSTERMANN NILS ET AL: "Insights into the phosphoryltransfer mechanism of human thymidylate kinase gained from crystal structures of enzyme complexes along the reaction coordinate.", STRUCTURE (LONDON), vol. 8, no. 6, 2000, pages 629 - 642, XP000982582, ISSN: 0969-2126 *

Also Published As

Publication number Publication date
EP1200565A2 (en) 2002-05-02
CA2378010A1 (en) 2001-02-15
AU781654B2 (en) 2005-06-02
WO2001011025A2 (en) 2001-02-15
AU6622700A (en) 2001-03-05
JP2003517818A (en) 2003-06-03

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