WO2001006004A3 - A method for amplifying low abundance nucleic acid sequences and means for performing said method - Google Patents

A method for amplifying low abundance nucleic acid sequences and means for performing said method Download PDF

Info

Publication number
WO2001006004A3
WO2001006004A3 PCT/EP2000/006887 EP0006887W WO0106004A3 WO 2001006004 A3 WO2001006004 A3 WO 2001006004A3 EP 0006887 W EP0006887 W EP 0006887W WO 0106004 A3 WO0106004 A3 WO 0106004A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid sequences
low abundance
amplifying low
abundance nucleic
Prior art date
Application number
PCT/EP2000/006887
Other languages
French (fr)
Other versions
WO2001006004A2 (en
Inventor
Peter Richardson
Peter Cox
Original Assignee
Univ Cambridge Tech
Peter Richardson
Peter Cox
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Cambridge Tech, Peter Richardson, Peter Cox filed Critical Univ Cambridge Tech
Priority to CA002378070A priority Critical patent/CA2378070A1/en
Priority to KR1020027000769A priority patent/KR20020034161A/en
Priority to EP00954533A priority patent/EP1196639A2/en
Priority to JP2001511213A priority patent/JP2003505034A/en
Priority to AU66956/00A priority patent/AU6695600A/en
Publication of WO2001006004A2 publication Critical patent/WO2001006004A2/en
Publication of WO2001006004A3 publication Critical patent/WO2001006004A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Abstract

The present invention relates to methods as well as to nucleic acid primers and kits containing the same for performing efficiently an amplification of nucleic acid sequences from a sample, particularly of nucleic acid sequences that are initially poorly represented in said sample.
PCT/EP2000/006887 1999-07-19 2000-07-19 A method for amplifying low abundance nucleic acid sequences and means for performing said method WO2001006004A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002378070A CA2378070A1 (en) 1999-07-19 2000-07-19 A method for amplifying low abundance nucleic acid sequences and means for performing said method
KR1020027000769A KR20020034161A (en) 1999-07-19 2000-07-19 A method for amplifying low abundance nucleic acid sequences and means for performing said method
EP00954533A EP1196639A2 (en) 1999-07-19 2000-07-19 A method for amplifying low abundance nucleic acid sequences and means for performing said method
JP2001511213A JP2003505034A (en) 1999-07-19 2000-07-19 Method for amplifying nucleic acid sequences with low abundance and means for performing the method
AU66956/00A AU6695600A (en) 1999-07-19 2000-07-19 A method for amplifying low abundance nucleic acid sequences and means for performing said method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14466699P 1999-07-19 1999-07-19
US60/144,666 1999-07-19

Publications (2)

Publication Number Publication Date
WO2001006004A2 WO2001006004A2 (en) 2001-01-25
WO2001006004A3 true WO2001006004A3 (en) 2001-08-09

Family

ID=22509592

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/006887 WO2001006004A2 (en) 1999-07-19 2000-07-19 A method for amplifying low abundance nucleic acid sequences and means for performing said method

Country Status (6)

Country Link
EP (1) EP1196639A2 (en)
JP (1) JP2003505034A (en)
KR (1) KR20020034161A (en)
AU (1) AU6695600A (en)
CA (1) CA2378070A1 (en)
WO (1) WO2001006004A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1275738A1 (en) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Method for random cDNA synthesis and amplification
US7414111B2 (en) 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
AU2002341752B2 (en) 2001-09-19 2008-04-03 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
GB0216026D0 (en) * 2002-07-10 2002-08-21 Cambridge Biotechnology Ltd Nucleic acid amplification method
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
EP1735459B1 (en) * 2004-04-07 2012-01-25 Exiqon A/S Methods for quantification of micrornas and small interfering rnas
KR100590621B1 (en) * 2004-09-21 2006-06-19 (주)지노믹트리 Method for Linear Amplification of RNA Using High-Heel Primer
JP4777631B2 (en) * 2004-09-27 2011-09-21 株式会社日立ハイテクノロジーズ Nucleic acid amplification analysis method and apparatus
CN101730847B (en) * 2007-04-05 2015-03-18 分类生物***有限公司 Compositions and methods of detection
CN102685048B (en) * 2012-05-28 2014-10-29 上海师范大学 Blind equalization algorithm for burst signal
CN107075732B (en) * 2014-11-25 2020-08-04 深圳华大智造科技有限公司 Method for constructing free DNA sequencing library

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991015601A1 (en) * 1990-04-05 1991-10-17 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Modified rna template-specific polymerase chain reaction
WO1998008973A1 (en) * 1996-08-29 1998-03-05 Cancer Research Campaign Technology Limited Global amplification of nucleic acids
WO2000008208A2 (en) * 1998-08-05 2000-02-17 Medical Research Council Reverse transcription and amplification processes and primers therefor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991015601A1 (en) * 1990-04-05 1991-10-17 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Modified rna template-specific polymerase chain reaction
WO1998008973A1 (en) * 1996-08-29 1998-03-05 Cancer Research Campaign Technology Limited Global amplification of nucleic acids
WO2000008208A2 (en) * 1998-08-05 2000-02-17 Medical Research Council Reverse transcription and amplification processes and primers therefor

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CHEUNG AND NELSON: "WHOLE GENOME AMPLIFICATION USING A DEGENERATE OLIGONUCLEOTIDE PRIMER ALLOWS HUNDREDS OF GENOTYPES TO BE PERFORMED ON LESS THAN ONE NANOGRAM OF GENOMIC DNA", PNAS, vol. 93, 1996, pages 14676 - 14679, XP002128316 *
DIXON ET AL.: "EXPRESSION PROFILING OF SINGLE CELLS USING 3 PRIME END AMPLIFICATION (TPEA) PCR", NUCLEIC ACIDS RESEARCH, vol. 26, no. 19, October 1998 (1998-10-01), pages 4426 - 4431, XP002128317 *
EBERWINE J ET AL: "ANALYSIS OF GENE EXPRESSION IN SINGLE LIVE NEURONS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 89, 1 April 1992 (1992-04-01), pages 3010 - 3014, XP002912945, ISSN: 0027-8424 *
FROUSSARD: "A RANDOM-PCR METHOD (rPCR) TO CONSTRUCT WHOLE cDNA LIBRARY FROM LOW AMOUNTS OF RNA", NUCLEIC ACIDS RESEARCH, vol. 20, no. 11, 1992, pages 2900, XP002128315 *
RICHARDSON ET AL.: "RELATING GENE EXPRESSION TO CELL FUNCTION USING A NOVEL SINGLE CELL PCR METHOD", EUROPEAN JOURNAL OF NEUROSCIENCE, vol. 10, no. supp. [10], August 1998 (1998-08-01), pages 10307, XP000982404 *
TELENIUS H ET AL: "DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR: GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER", GENOMICS,US,ACADEMIC PRESS, SAN DIEGO, vol. 13, 1992, pages 718-725, XP000199116, ISSN: 0888-7543 *
ZHAO ET AL.: "3'-END cDNA POOL SUITABLE FOR DIFFERENTIAL DISPLAY FROM A SMALL NUMBER OF CELLS", BIOTECHNIQUES, vol. 24, May 1998 (1998-05-01), pages 842 - 852, XP002128314 *
ZHAO ET AL.: "NEW PRIMER STRATEGY IMPROVES PRECISION OF DIFFERENTIAL DISPLAY", BIOTECHNIQUES, vol. 18, no. 5, 1995, pages 842 - 850, XP002128267 *

Also Published As

Publication number Publication date
KR20020034161A (en) 2002-05-08
EP1196639A2 (en) 2002-04-17
CA2378070A1 (en) 2001-01-25
AU6695600A (en) 2001-02-05
JP2003505034A (en) 2003-02-12
WO2001006004A2 (en) 2001-01-25

Similar Documents

Publication Publication Date Title
WO2004081228A3 (en) Real time gene expression profiling
WO2006039563A3 (en) Method for providing dna fragments derived from an archived sample
WO2003054511A3 (en) Methods for quantitating nucleic acids using coupled ligation and amplification
WO2006071776A3 (en) Ligation-based rna amplification
WO2002052031A3 (en) Nucleic acid amplification
WO2001071027A3 (en) mRNA AMPLIFICATION
WO2006010610A3 (en) Method for determining the abundance of sequences in a sample
WO2004074503A3 (en) Nucleic acid sequencing methods, kits and reagents
WO2002101022A3 (en) Method for rapid amplification of dna
WO2005003375A3 (en) Methods of amplifying and sequencing nucleic acids
WO2006081284A3 (en) Methods for quantitating small rna molecules
BG107861A (en) Isothermal amplification of nucleic acids on a solid support
WO2007030759A3 (en) Improved nucleic acid amplification procedure
WO2003008622A3 (en) Exponential nucleic acid amplification using nicking endonucleases
WO2007086935A3 (en) Methods of amplifying and sequencing nucleic acids
HK1046021A1 (en) Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2005010209A3 (en) Method for the reverse transcription and/or amplification of nucleic acids
WO2006102309A3 (en) Methods, compositions, and kits for detection of micro rna
WO2004092418A3 (en) Global amplification using a randomly primed composite primer
AU4969996A (en) Methods for the isothermal amplification of nucleic acid molecules
WO2002072773A3 (en) Methods and compositions for amplification of rna sequences
WO2003033732A3 (en) Real-time detection of dna amplification products
AU2001244677A1 (en) Method of amplifying nucleic acid by using double-stranded nucleic acid as template
WO2006003439A3 (en) Method for stabilising reagents which are useful for nucleic acid amplification
WO2006076017A3 (en) Methods and kits for identifying target nucleotides in mixed populations

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2378070

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 1020027000769

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: 66956/00

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2000954533

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000954533

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1020027000769

Country of ref document: KR

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 10019906

Country of ref document: US

WWW Wipo information: withdrawn in national office

Ref document number: 2000954533

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1020027000769

Country of ref document: KR