WO2001000796A3 - Glycosyltransferases of helicobacter pylori - Google Patents

Glycosyltransferases of helicobacter pylori Download PDF

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Publication number
WO2001000796A3
WO2001000796A3 PCT/CA2000/000777 CA0000777W WO0100796A3 WO 2001000796 A3 WO2001000796 A3 WO 2001000796A3 CA 0000777 W CA0000777 W CA 0000777W WO 0100796 A3 WO0100796 A3 WO 0100796A3
Authority
WO
WIPO (PCT)
Prior art keywords
glycosyltransferases
helicobacter pylori
disclosed
polynucleotide sequences
polypeptides
Prior art date
Application number
PCT/CA2000/000777
Other languages
French (fr)
Other versions
WO2001000796A2 (en
Inventor
Susan M Logan
Warren Wakarchuk
Wayne Conlan
Mario A Monteiro
Eleonora Altman
Koji Hiratsuka
Original Assignee
Ca Nat Research Council
Susan M Logan
Warren Wakarchuk
Wayne Conlan
Mario A Monteiro
Eleonora Altman
Koji Hiratsuka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Nat Research Council, Susan M Logan, Warren Wakarchuk, Wayne Conlan, Mario A Monteiro, Eleonora Altman, Koji Hiratsuka filed Critical Ca Nat Research Council
Priority to AU56684/00A priority Critical patent/AU5668400A/en
Priority to CA002377427A priority patent/CA2377427A1/en
Publication of WO2001000796A2 publication Critical patent/WO2001000796A2/en
Priority to EP01947090A priority patent/EP1299527A2/en
Priority to AU2001268885A priority patent/AU2001268885A1/en
Priority to EP01947091A priority patent/EP1299549A1/en
Priority to US10/451,685 priority patent/US20040110261A1/en
Priority to CA002417699A priority patent/CA2417699A1/en
Priority to PCT/CA2001/000970 priority patent/WO2002000888A1/en
Priority to CA002417692A priority patent/CA2417692A1/en
Priority to AU2001268886A priority patent/AU2001268886A1/en
Priority to PCT/CA2001/000969 priority patent/WO2002000851A2/en
Publication of WO2001000796A3 publication Critical patent/WO2001000796A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Novel isolated polynucleotides encoding glycosyltransferases involved in the biosynthesis of the lipopolysaccharide of Helicobacter pylori, together with recombinant DNA constructs and vectors containing polynucleotide sequences encoding such glycosyltransferases are disclosed. These nucleic acid constructs and vectors may be used for the preparation of glycosyltransferases they encode, by expressing the coding polynucleotide sequences in suitable host cells. Also disclosed are isolated polypeptides having enzymatic activity of helicobacterial glycosyltransferases. Such polypeptides are particularly useful for screening of modulators of their enzymatic activity, in particular enzymatic inhibitors having potential antibacterial activity.
PCT/CA2000/000777 1999-06-28 2000-06-28 Glycosyltransferases of helicobacter pylori WO2001000796A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
AU56684/00A AU5668400A (en) 1999-06-28 2000-06-28 Glycosyltransferases of helicobacter pylori as a new target in prevention and treatment of h. pylori infections
CA002377427A CA2377427A1 (en) 1999-06-28 2000-06-28 Glycosyltransferases of helicobacter pylori as a new target in prevention and teatment of h. pylori infections
PCT/CA2001/000969 WO2002000851A2 (en) 2000-06-28 2001-06-28 Helicobacter dd-heptosyltransferase
EP01947091A EP1299549A1 (en) 2000-06-28 2001-06-28 Helicobacter pylori heptosyltransferase polynucleotides and polypeptides
AU2001268885A AU2001268885A1 (en) 2000-06-28 2001-06-28 Helicobacter dd-heptosyltransferase
EP01947090A EP1299527A2 (en) 2000-06-28 2001-06-28 Helicobacter dd-heptosyltransferase
US10/451,685 US20040110261A1 (en) 2000-06-28 2001-06-28 Helicobacter dd-heptosyltransferase
CA002417699A CA2417699A1 (en) 2000-06-28 2001-06-28 Helicobacter pylori heptosyl transferase polypeptides
PCT/CA2001/000970 WO2002000888A1 (en) 2000-06-28 2001-06-28 Helicobacter pylori heptosyl transferase polypeptides
CA002417692A CA2417692A1 (en) 2000-06-28 2001-06-28 Helicobacter dd-heptosyltransferase
AU2001268886A AU2001268886A1 (en) 2000-06-28 2001-06-28 Helicobacter pylori heptosyl transferase polypeptides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14082099P 1999-06-28 1999-06-28
US60/140,820 1999-06-28

Publications (2)

Publication Number Publication Date
WO2001000796A2 WO2001000796A2 (en) 2001-01-04
WO2001000796A3 true WO2001000796A3 (en) 2001-08-09

Family

ID=22492927

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2000/000777 WO2001000796A2 (en) 1999-06-28 2000-06-28 Glycosyltransferases of helicobacter pylori

Country Status (3)

Country Link
AU (1) AU5668400A (en)
CA (1) CA2377427A1 (en)
WO (1) WO2001000796A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11221079A (en) 1998-02-04 1999-08-17 Kyowa Hakko Kogyo Co Ltd Transglycosidase and dna encoding the same enzyme
EP1299549A1 (en) * 2000-06-28 2003-04-09 National Research Council of Canada Helicobacter pylori heptosyltransferase polynucleotides and polypeptides
EP1299527A2 (en) * 2000-06-28 2003-04-09 National Research Council Of Canada Helicobacter dd-heptosyltransferase
GB0017149D0 (en) * 2000-07-12 2000-08-30 Chiron Spa Helicobacter pylori mutants
CN110997714B (en) * 2017-02-17 2024-05-03 赛诺菲 Multispecific binding molecules with specificity for dystrophin proteoglycans and laminin-2

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040893A1 (en) * 1995-06-07 1996-12-19 Astra Aktiebolag Nucleic acid and amino acid sequences relating to helicobacter pylori for diagnostics and therapeutics
WO1999040205A1 (en) * 1998-02-04 1999-08-12 Kyowa Hakko Kogyo Co., Ltd. Glycosyltransferase and dna encoding the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040893A1 (en) * 1995-06-07 1996-12-19 Astra Aktiebolag Nucleic acid and amino acid sequences relating to helicobacter pylori for diagnostics and therapeutics
WO1999040205A1 (en) * 1998-02-04 1999-08-12 Kyowa Hakko Kogyo Co., Ltd. Glycosyltransferase and dna encoding the same
EP1054062A1 (en) * 1998-02-04 2000-11-22 Kyowa Hakko Kogyo Co., Ltd. Glycosyltransferase and dna encoding the same

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHAN N W ET AL: "THE BIOSYNTHESIS OF LEWIS X IN HELICOBACTER PYLORI", GLYCOBIOLOGY,GB,IRL PRESS,, vol. 5, no. 7, 1995, pages 683 - 688, XP002920175, ISSN: 0959-6658 *
DATABASE EMBL 25 August 1997 (1997-08-25), TOMB J -F ET AL: "Helicobacter pylori 26695 section 72 of 134 of the complete genome.", XP002155934 *
MARTIN S L ET AL: "Lewis X biosynthesis in Helicobacter pylori", JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 272, no. 34, 22 August 1997 (1997-08-22), pages 21349 - 21356, XP002085414, ISSN: 0021-9258 *
TOMB J -F ET AL: "THE COMPLETE GENOME SEQUENCE OF THE GASTRIC PATHOGEN HELICOBACTER PYLORI", NATURE,GB,MACMILLAN JOURNALS LTD. LONDON, vol. 388, no. 6642, 7 August 1997 (1997-08-07), pages 539 - 547,TABEL, XP002062106, ISSN: 0028-0836 *
WANG G ET AL: "MOLECULAR GENETIC BASIS FOR THE VARIABLE EXPRESSION OF LEWIS Y ANTIGEN IN HELICOBACTER PYLORI: ANALYSIS OF THE ALPHA(1,2) FUCOSYLTRANSFERASE GENE", MOLECULAR MICROBIOLOGY,GB,BLACKWELL SCIENTIFIC, OXFORD, vol. 31, no. 4, February 1999 (1999-02-01), pages 1265 - 1274, XP000889904, ISSN: 0950-382X *

Also Published As

Publication number Publication date
AU5668400A (en) 2001-01-31
WO2001000796A2 (en) 2001-01-04
CA2377427A1 (en) 2001-01-04

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