WO2000076515A1 - Antagonistes du recepteur il-8 - Google Patents

Antagonistes du recepteur il-8 Download PDF

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Publication number
WO2000076515A1
WO2000076515A1 PCT/US2000/016510 US0016510W WO0076515A1 WO 2000076515 A1 WO2000076515 A1 WO 2000076515A1 US 0016510 W US0016510 W US 0016510W WO 0076515 A1 WO0076515 A1 WO 0076515A1
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Prior art keywords
optionally substituted
alkyl
4alkyl
heterocyclic
heteroaryl
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PCT/US2000/016510
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English (en)
Inventor
Katherine L. Widdowson
Gregory Martin Benson
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Smithkline Beecham Corporation
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Priority to BR0011567-3A priority Critical patent/BR0011567A/pt
Application filed by Smithkline Beecham Corporation filed Critical Smithkline Beecham Corporation
Priority to JP2001502848A priority patent/JP2003501470A/ja
Priority to KR1020017016141A priority patent/KR20020012275A/ko
Priority to CA002377391A priority patent/CA2377391A1/fr
Priority to PL00352222A priority patent/PL352222A1/xx
Priority to NZ515609A priority patent/NZ515609A/en
Priority to IL14651500A priority patent/IL146515A0/xx
Priority to MXPA01013089A priority patent/MXPA01013089A/es
Priority to EP00944689A priority patent/EP1185271A4/fr
Priority to AU58750/00A priority patent/AU767000B2/en
Publication of WO2000076515A1 publication Critical patent/WO2000076515A1/fr
Priority to NO20016102A priority patent/NO20016102L/no
Priority to AU2004200500A priority patent/AU2004200500A1/en

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Definitions

  • This invention relates to a novel group of phenyl urea compounds processes for the preparation thereof, the use thereof in treating IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , , NAP-2, and ENA-78 mediated diseases and pharmaceutical compositions for use in such therapy.
  • Interleukin-8 such as neutrophil attractant/activation protein- 1 (NAP-1), monocyte derived neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF), and T-cell lymphocyte chemotactic factor.
  • Interleukin-8 is a chemoattractant for neutrophils, basophils, and a subset of T-cells. It is produced by a majority of nucleated cells including macrophages, fibroblasts, endothelial and epithelial cells exposed to TNF, IL-l ⁇ L-l ⁇ or LPS, and by neutrophils themselves when exposed to LPS or chemotactic factors such as FMLP.
  • GRO ⁇ , GRO ⁇ , GRO ⁇ and NAP-2 also belong to the chemokine ⁇ family.
  • chemokines Like IL-8 these chemokines have also been referred to by different names. For instance GRO ⁇ , ⁇ , ⁇ have been referred to as MGSA ⁇ , ⁇ and ⁇ respectively (Melanoma Growth Stimulating Activity), see Richmond et al, J. Cell Physiology 129, 375 (1986) and Chang et al, J. Immunol 148, 451 (1992). All of the chemokines of the ⁇ - family which possess the ELR motif directly preceding the CXC motif bind to the IL-8 ⁇ receptor.
  • IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2 and ENA-78 stimulate a number of functions in vitro. They have all been shown to have chemoattractant properties for neutrophils, while IL-8 and GRO ⁇ have demonstrated T-lymphocytes, and basophiles chemotactic activity.
  • IL-8 can induce histamine release from basophils from both normal and atopic individuals
  • GRO ⁇ and IL-8 can in addition, induce lysozomal enzyme release and respiratory burst from neutrophils.
  • IL-8 has also been shown to increase the surface expression of Mac- 1 (CD1 lb/CD 18) on neutrophils without de novo protein synthesis.
  • ELR chemokines (those containing the amino acids ELR motif just prior to the CXC motif) have also been implicated in angiostasis. Strieter et al., Science 258, 1798 (1992).
  • IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , and NAP-2 induce neutrophil shape change, chemotaxis, granule release, and respiratory burst, by binding to and activating receptors of the seven-transmembrane, G-protein-linked family, in particular by binding to IL-8 receptors, most notably the ⁇ -receptor.
  • the development of non-peptide small molecule antagonists for members of this receptor family has precedent. For a review see R. Freidinger in: Progress in Drug Research, Vol. 40, pp.
  • IL-8 receptor represents a promising target for the development of novel anti-inflammatory agents.
  • Two high affinity human IL-8 receptors (77% homology) have been characterized: IL-8R ⁇ , which binds only IL-8 with high affinity, and IL-8R ⁇ , which has high affinity for IL-8 as well as for GRO ⁇ , GRO ⁇ , GRO ⁇ and NAP-2.
  • IL-8R ⁇ Two high affinity human IL-8 receptors (77% homology) have been characterized: IL-8R ⁇ , which binds only IL-8 with high affinity, and IL-8R ⁇ , which has high affinity for IL-8 as well as for GRO ⁇ , GRO ⁇ , GRO ⁇ and NAP-2.
  • This invention provides for a method of treating a chemokine mediated disease, wherein the chemokine is one which binds to an IL-8 ⁇ or ⁇ receptor and which method comprises administering an effective amount of a compound of Formula (I) or a pharmaceutically acceptable salt thereof.
  • the chemokine is IL-8.
  • This invention also relates to a method of inhibiting the binding of IL-8 to its receptors in a mammal in need thereof which comprises administering to said mammal an effective amount of a compound of Formula (I).
  • Compounds of Formula (I) useful in the present invention are represented by the structure:
  • X is oxygen or sulfur
  • R is (CR 8 R 8 )r C(O) 2 H, (CR 8 R 8 )r NH-C(O)R a , (CR 8 R 8 )r C(O)NR 6 'R7',
  • Ri is independently selected from hydrogen; halogen; nitro; cyano; halosubstituted Ci-io alkyl; Ci -io alkyl; C 2 -io alkenyl; Ci-io alkoxy; halosubstituted Ci -io alkoxy; azide; (CR R )q S(O) t R4; hydroxy; hydroxy Ci-_talkyl; aryl; aryl Cj-4 alkyl; aryloxy; aryl C ⁇ _4 alkyloxy; heteroaryl; heteroarylalkyl; heterocyclic, heterocyclic Ci_4alkyl; heteroaryl Ci-4 alkyloxy; aryl
  • Ri moieties together may form O-(CH 2 ) s O- or a 5 to 6 membered saturated or unsaturated ring; and wherein the aryl, heteroaryl, and heterocyclic containing moieties may be optionally substituted; provided that there is no ionizable hydrogen having a pKa of 3 to 10 in the 2-position of the phenyl ring; n is an integer having a value of 1 to 3; m is an integer having a value of 1 to 3; q is 0, or an integer having a value of 1 to 10; r is 0 or an integer of 1 to 4; s is
  • R4 and R5 are independently hydrogen, optionally substituted Cj-4 alkyl, optionally substituted aryl, optionally substituted aryl C ⁇ -4alkyl, optionally substituted heteroaryl, optionally substituted heteroaryl C ⁇ _4alkyl, heterocyclic, heterocyclic Ci -4 alkyl, or R4 and R5 together with the nitrogen to which they are attached form a 5 to 7 member ring which may optionally comprise an additional heteroatom selected from O/N/S; R6 and R7 are independently hydrogen or a C ⁇ _4 alkyl group, or R6 and R7 together with the nitrogen to which they are attached form a 5 to 7 member ring which ring may optionally contain an additional heteroatom which heteroatom is selected from oxygen, nitrogen or sulfur; R6' and R7' are independently hydrogen, Ci-4 alkyl, aryl, arylCi-4alkyl, arylC 2 _4alkenyl, heteroaryl, heteroarylC ⁇ _4alkyl, heteroarylC 2 -4 al
  • R 8 is independently selected from hydrogen or C 1-4 alkyl
  • RlO is Ci-10 alkyl C(O) 2 R 8 ;
  • Rl 1 is hydrogen, Ci-4 alkyl, optionally substituted aryl, optionally substituted aryl Ci-4alkyl, optionally substituted heteroaryl, optionally substituted heteroarylCi-4alkyl, optionally substituted heterocyclic, or optionally substituted heterocyclicC 1 _4alkyl;
  • Rl 2 is hydrogen, C i-io alkyl, optionally substituted aryl or optionally substituted arylalkyl;
  • Rl3 and R 14 are independently hydrogen, optionally substituted C1 -4 alkyl, or one of R13 and R14 may be optionally substituted aryl;
  • Rl7 is Ci-4alkyl, aryl, arylalkyl, heteroaryl, heteroarylCi-4alkyl, heterocyclic, or heterocyclicC ⁇ _4alkyl, wherein the aryl, heteroaryl and heterocyclic rings may all be optionally substituted;
  • R a is an alkyl, aryl, aryl Ci-4alkyl, heteroaryl, heteroaryl Ci-4alkyl, heterocyclic, or a heterocyclic Ci-4alkyl moiety, wherein all of these moieties may be optionally substituted;
  • R is a NR6R7- alkyl, aryl, arylCi-4alkyl, arylC 2 -4alkenyl, heteroaryl, heteroarylCi-4alkyl, heteroarylC 2 -4 alkenyl,
  • R c is alkyl, aryl, arylCi-4alkyl, arylC 2 -4alkenyl, heteroaryl, heteroarylCi-4alkyl, heteroarylC 2 -4alkenyl, heterocyclic, heterocyclic C ⁇ _4alkyl, or a heterocyclic
  • R d is NR5R7, alkyl, arylC ⁇ .4 alkyl, arylC 2 _4 alkenyl, heteroaryl, heteroaryl-C 1-4 alkyl, heteroarylC 2 _4 alkenyl, heterocyclic, heterocyclicC ⁇ _4 alkyl, wherein the aryl, heteroaryl and heterocyclic containing rings may be optionally substituted;
  • the E containing ring is optionally selected from
  • R 2 0 is Wi , optionally substituted heteroaryl, optionally substituted C5_ cycloalkyl, optionally substituted C I _I Q alkyl, optionally substituted C 2 .I Q alkenyl, or an optionally substituted C 2 _ ⁇ o alkynyl;
  • the E' containing ring is optionally selected from
  • the compounds of Formula (I) may also be used in association with the veterinary treatment of mammals, other than humans, in need of inhibition of IL-8 or other chemokines which bind to the IL-8 ⁇ and ⁇ receptors.
  • Chemokine mediated diseases for treatment, therapeutically or prophylactically, in animals include disease states such as those noted herein in the Methods of Treatment section.
  • R is (CR 8 R 8 )r C(O) 2 H, (CR 8 R 8 )r NH-C(O)R a ,
  • r is 0 or an integer of 1 to 4, preferably 0.
  • X 2 is oxygen or sulfur, preferably oxygen, wherein r is 0 or an integer having a value of 1 to 4.
  • R6 and R7 are independently hydrogen or a Ci-4 alkyl group, or R6 and R7 together with the nitrogen to which they are attached form a 5 to 7 member ring which ring may optionally contain an additional heteroatom which heteroatom is selected from oxygen, nitrogen or sulfur.
  • R6' and R7' are hydrogen, C1-.4 alkyl, aryl, arylC ⁇ _4alkyl, arylC 2 -4alkenyl.
  • heteroaryl heteroarylCi-4alkyl, heteroarylC 2 -4 alkenyl, heterocyclic, heterocyclic C ⁇ _4alkyl, or a heterocyclic C 2 -4alkenyl moiety, provided that one of R6' and R7' are hydrogen, but not both of R6' and R7'.
  • R a is an alkyl, aryl, arylCi_4alkyl, heteroaryl, heteroarylCi-4alkyl, heterocyclic, or a heterocyclic Ci-4alkyl moiety, wherein all of these moieties may be optionally substituted as defined herein.
  • R b is a NR6R7, alkyl, aryl, arylCi-4alkyl, arylC 2 -4alkenyl, heteroaryl, heteroarylCi-4alkyl, heteroarylC 2 -4 alkenyl, heterocyclic, heterocyclic C ⁇ _4--lkyl, or a heterocyclic C 2 -4alkenyl moiety, or camphor, wherein the alkyl, aryl, heteroaryl and heterocyclic containing moieties may all be optionally substituted one to three times independently by halogen; nitro; halosubstituted Ci-4 alkyl, such as CF3; C1-4 alkyl, such as methyl; Ci-4 alkoxy, such as methoxy; NR9C(O)R a ; C(O)NR ⁇ R7; S(O)3H; or C(O)OC ⁇ _4 alkyl.
  • R b is preferably an optionally substituted phenyl, benzyl, or styryl.
  • R b is preferably an optionally substituted thiazole, an optionally substituted thienyl, or an optionally substituted quinolinyl ring.
  • R9 is hydrogen or a C1 -.4 alkyl group, preferably a hydrogen.
  • R9 is in the substituent group NR9C(O)R
  • R is preferably an alkyl group, such as methyl.
  • R c is hydrogen, alkyl, aryl, arylC ⁇ _4alkyl, arylCi-4alkenyl, heteroaryl, heteroarylCi-4alkyl, heteroarylC ⁇ _4alkenyl, heterocyclic, or heterocyclic Ci-4alkyl, or a heterocyclic C ⁇ _4alkenyl moiety, all of which may be optionally substituted one to three times independently by halogen, nitro, halosubstituted Ci-4 alkyl, C1-4 alkyl, C1-4 alkoxy, NR9C(O)R a , C(O)NR6R7, S(O)3H, or C(O)OC ⁇ _4 alkyl.
  • Rc is an optionally substituted phenyl.
  • the E containing ring is an optionally substituent which is selected from
  • R ⁇ is independently selected from hydrogen; halogen; nitro; cyano; halosubstituted Ci-io alkyl, such as CF3; Cj-io alkyl, such as methyl, ethyl, isopropyl, or n-propyl; C 2 -io alkenyl; Ci-io alkoxy, such as methoxy, or ethoxy; halosubstituted alkoxy, such as trifluoromethoxy; azide; (CR 8 R )q S(O) ⁇ R4; hydroxy; hydroxy C ⁇ _4alkyl, such as methanol or ethanol; aryl, such as phenyl or naphthyl; aryl Ci-4 alkyl, such as benzyl; aryloxy, such as phenoxy; aryl Cj-4 alkyloxy, such as benzyloxy; heteroaryl; heteroarylalkyl; heteroaryl C ⁇
  • t is 0, an integer having a value of 1 or 2.
  • s is an integer having a value of 1 to 3.
  • q is 0, or an integer having a value of 1 to 10.
  • s is preferably 1.
  • R ⁇ forms an additional saturated or unsaturated ring, it is preferably a 6 membered ring resulting in a naphthylene ring system.
  • saturated and unsaturated ring systems may be optionally substituted independently, 1 to 3 times by the other R ⁇ moieties as defined above.
  • R4 and R5 are independently hydrogen, optionally substituted C1-.4 alkyl, optionally substituted aryl, optionally substituted aryl C ⁇ -4alkyl, optionally substituted heteroaryl, optionally substituted heteroaryl C ⁇ -4alkyl, heterocyclic, or heterocyclicCi-4 alkyl, or R4 and R5 together with the nitrogen to which they are attached form a 5 to 7 member ring which may optionally comprise an additional heteroatom selected from O/N/S.
  • R 8 is suitably independently selected from hydrogen or Ci-4 alkyl.
  • RlO is suitably C ⁇ io alkyl C(O) 2 R 8 , such as CH 2 C(O) 2 H or CH 2 C(O) 2 CH3.
  • Rl l is suitably independently hydrogen, C1 -4 alkyl, aryl, aryl C1 -4 alkyl, heteroaryl, heteroaryl Ci-4- ⁇ lkyl, heterocyclic, or heterocyclic C1 -4alkyl.
  • Rl 2 is suitably hydrogen, Cl-10 alkyl, optionally substituted aryl or optionally substituted arylalkyl.
  • Rl7 is suitably Ci-4alkyl, aryl, arylalkyl, heteroaryl, heteroarylC ⁇ _4alkyl, heterocyclic, or heterocyclicCi-4alkyl, wherein the aryl, heteroaryl and heterocyclic rings may all be optionally substituted.
  • Ri is halogen, cyano, nitro, CF3, C(O)NR4R5, alkenyl C(O)NR4R5, C(O) R4R10, alkenyl C(O)ORi 2 , heteroaryl, heteroarylalkyl , heteroaryl alkenyl, or S(O)NR4R5, and preferably R4 and R5 are both hydrogen or one of R4 and R5 is phenyl.
  • a preferred ring substitution for the Rj group is in the 4-position of the phenyl ring.
  • R is (CR 8 R 8 ) r OH, (CR 8 R 8 ) r SH or (CR 8 R 8 ) r NHS(O) 2 R b than R ⁇ is preferably substituted in the 4- position, or disubstituted in the 2,4-position.
  • the R ⁇ substituent group is an electron withdrawing moiety, such as nitro, halogen, cyano, trifluoromethyl, or C(O)NR4R5.
  • R ⁇ is preferably hydrogen, or R ⁇ is preferably substituted in the 4-position, more preferably substituted by trifluoromethyl or chloro.
  • R13 and R14 are independently hydrogen, an optionally substituted C1-.4 alkyl which may be straight or branched as defined herein, or one of R13 and Ri 4 is an optionally substituted aryl.
  • the alkyl moiety may be substituted one to three times independently by halogen; halosubstituted C ⁇ _4 alkyl such as trifluoromethyl; hydroxy; hydroxy Ci-4alkyl, Ci-4 alkoxy; such as methoxy, or ethoxy; halosubstituted Ci-io alkoxy; S(O)tR4; aryl; NR4R5; NHC(O)R4; C(O)NR4R5; or C(O)OR 8 .
  • v is 0 or an integer having a value of 1 to 4.
  • Y is independently selected from hydrogen; halogen; nitro; cyano; halosubstituted Ci-io alkyl; Ci-io alkyl; C 2 -io alkenyl; Cj-io alkoxy; halosubstituted Ci-io alkoxy; azide; (CR R 8 )q S(O)tR4; hydroxy; hydroxyCi-4alkyl; aryl; aryl Ci-4 alkyl; aryloxy; arylCi-4 alkyloxy; heteroaryl; heteroarylalkyl; heteroaryl Ci-4 alkyloxy; heterocyclic, heterocyclic C ⁇ _4alkyl; aryl C 2 -io alkenyl; heteroaryl C 2 -io alkenyl; heterocyclic C 2 _ ⁇ o alkenyl; (CR 8 Rg)q NR4R5 ; C 2 .10 alkenyl C(O)NR4R5. (CR 8 Rg)q
  • C(O)NR4Rl ⁇ ; S(O) 3 H; S(O) 3 R8, such as S(O) 3 H; (CR 8 R 8 )q C(O)R ⁇ 1; C2-10 alkenyl C(O)Ri 1; C2-10 alkenyl C(O)OR ⁇ 1 ; (CR 8 R 8 )q C(O)OR ⁇ 2 ; (CR 8 R 8 )q OC(O) R11 ; (CR 8 R 8 )q NR4C(O)Rn ; (CR 8 R 8 )q NHS(O) 2 R d ; (CR 8 R 8 )q S(O) 2 NR4R5 or two Y moieties together may form O-(CH 2 ) s O- or a 5 to 6 membered saturated or unsaturated ring.
  • the aryl, heteroaryl, and heterocyclic containing moieties may all be optionally substituted.
  • s is preferably 1.
  • Y forms an additional unsaturated ring, it is preferably 6 membered resulting in a naphthylene ring system.
  • These saturated and unsaturated rings may be optionally substituted 1 to 3 times by the other Y moieties as defined above.
  • R d is a NR6R7, alkyl, aryl C1 -4 alkyl, arylC 2 -4 alkenyl, heteroaryl, heteroaryl-C 1-4 alkyl, heteroarylC _4 alkenyl, heterocyclic, heterocyclicCi-4 alkyl, or heterocyclic C 2 -4 alkenyl moiety, wherein the aryl, heteroaryl, and heterocyclic containing moieties may all be optionally substituted as defined herein.
  • Y is preferably a halogen, C1 -4 alkoxy, optionally substituted aryl, optionally substituted aryloxy or arylalkoxy, methylene dioxy, NR4R5, thio Ci -4alkyl, thioaryl, halosubstituted alkoxy, optionally substituted C1 -4 alkyl, or hydroxy alkyl.
  • Y is more preferably a mono-substituted halogen, disubstituted halogen, mono-substituted alkoxy, disubstituted alkoxy, methylenedioxy, aryl, or alkyl.
  • these groups are mono or di-substituted in the 2'- position or 2'-, 3'-position of the phenyl ring. While Y may be substituted in any of the 5 ring positions, preferably when R is (CR 8 R 8 )rC(O) 2 H, Y is preferably mono-substituted in the 2 '-position or
  • R ⁇ and Y can both be hydrogen, it is preferred that at least one of the rings be substituted, and more preferably that both rings are substituted.
  • X is suitably oxygen or sulfur, preferably oxygen.
  • the E and E' rings denoted by its point of attachment through the asterix (*) may optionally be present. If it is not present the ring is a phenyl moiety which is substituted by the R ⁇ and Y terms as shown herein.
  • the E and E' ring may be substituted by the R ⁇ and Y moiety, respectively, in any ring, saturated or unsaturated, and is shown for purposes herein substituted only in the unsaturated ring(s).
  • R 2 Q is WJ , an optionally substituted heteroaryl, an optionally substituted C5_ 8 cycloalkyl, an optionally substituted C J ' IQ alkyl, an optionally substituted C 2 _I Q alkenyl, or an optionally substituted C 2 .
  • I Q alkynyl.
  • R 2 o is an optionally substituted C5_ 8 cycloalkyl ring
  • the ring may be substituted by (Y) n as defined above.
  • R 2 o is an optionally substituted C J. ⁇ Q alkyl, an optionally substituted
  • C 2 -10 alkenyl, or an optionally substituted C 2 _I Q alkynyl these moieties may be optionally substituted one or more times independently by halogen; nitro; cyano; halosubstituted Ci -io alkyl, such as trifluoromethyl; Ci-io alkoxy; halosubstituted Ci-io alkoxy; S(O)tR4; hydroxy; hydroxy C ⁇ -4alkyl;; aryloxy; arylCi-4 alkyloxy; heteroaryloxy; heteroaryl Ci-4 alkyloxy; heterocyclic, heterocyclic C ⁇ _4alkyl; heterocyclicoxy; heterocyclic C1.4 alkyloxy; NR4R5; C(O)NR4R5; C(O)NR4R ⁇ o;
  • R 2 o is an optionally substituted C 2 _ I Q alkenyl, or an optionally substituted C 2 _JO alkynyl these moieties may also, in addition to those moieties noted above, be optionally substituted with aryl, aryl Ci-4 alkyl, heteroaryl, or a heteroaryl Ci-4 alkyl (and wherein these aryl and heteroaryl containing rings may be optionally substituted).
  • Wi is , or
  • the E' containing ring is optionally selected from
  • R Q when R Q is a heteroaryl (HET) ring, it is suitably a heteroaryl ring or ring system.
  • HET heteroaryl
  • the ring containing the heteroatom does not need to be directly attached to the urea moiety or the (CRj 3Ri4) v term. All the rings in this ring system may be optionally substituted by the (Y( n ) ) term as defined above.
  • the HET moiety is a pyridyl, which may be 2-, 3- or 4-pyridyl.
  • the ring is a multi system ring it is preferably a benzimidazole, dibenzothiophene, or indole ring.
  • heterocyclic rings of interest include, but are not limited to thiophene, furan, pyrimidine, pyrrole, pyrazole, quinoline, isoquinoline, quinazolinyl, pyridine, oxazole, thiazole, thiadiazole, triazole, or imidazole.
  • R 2 0 is preferably an optionally substituted phenyl, allyl, C i Q alkyl, ethoxy carbonyl ethyl, dimethylacetal, 2-methoxy isopropyl, or 2-methoxy ethyl group.
  • Exemplified compounds of Formula (I) include: N-(3-Carboxyphenyl)-N'-(2-bromophenyl)urea N-(3-Carboxymethylphenyl)-N'-(2-bromophenyl)urea N-(3-Carboxymethylphenyl)-N'-(2,3-dichlorophenyl)urea N-(3-Carboxyphenyl)-N'-(2,3-dichlorophenyl)urea N-[3-(2-Carboxyethyl)phenyl]-N'-(2,3-dichlorophenyl) urea N-(2,4-Dichloro-3-carboxy)-N'-(2-bromophenyl) urea N-(4-Chloro-3-carboxyphenyl)-N'-(2-bromophenyl)urea N-(4-Chloro-3-carboxyphenyl)-N'-(2-
  • halogen such as fluorine, chlorine, bromine or iodine
  • hydroxy hydroxy substituted Ci-ioalkyl
  • Cj-io alkoxy such as methoxy or ethoxy
  • S(O) m ' Ci -io alkyl wherein m' is 0, 1 or 2, such as methyl thio, methyl sulfinyl or methyl sulfonyl
  • amino, mono & di-substituted amino such as in the NR4R5 group; NHC(O)R4; C(O)NR4R5; C(O)OH; S(O) 2 NR4R5; NHS(O) 2 R 2
  • Ci-io alkyl such as methyl, ethyl, propyl, isopropyl, or t-butyl
  • halosubstituted C ⁇ _ ⁇ o alkyl such as fluorine, chlorine, bromine or iodine
  • hydroxy hydroxy substitute
  • Suitable pharmaceutically acceptable salts are well known to those skilled in the art and include basic salts of inorganic and organic acids, such as hydrochloric acid, hydrobromic acid, sulphuric acid, phosphoric acid, methane sulphonic acid, ethane sulphonic acid, acetic acid, malic acid, tartaric acid, citric acid, lactic acid, oxalic acid, succinic acid, fumaric acid, maleic acid, benzoic acid, salicylic acid, phenylacetic acid and mandelic acid.
  • pharmaceutically acceptable salts of compounds of Formula (I) may also be formed with a pharmaceutically acceptable cation, for instance, if a substituent group comprises a carboxy moiety.
  • Suitable pharmaceutically acceptable cations are well known to those skilled in the art and include alkaline, alkaline earth, ammonium and quaternary ammonium cations.
  • halo all halogens, that is chloro, fluoro, bromo and iodo.
  • cycloalkyl is used herein to mean cyclic radicals, preferably of 3 to 8 carbons, including but not limited to cyclopropyl, cyclopentyl, cyclohexyl, and the like.
  • alkenyl is used herein at all occurrences to mean straight or branched chain radical of 2-10 carbon atoms, unless the chain length is limited thereto, including, but not limited to ethenyl, 1-propenyl, 2-propenyl, 2-methyl-l-propenyl, 1-butenyl, 2-butenyl and the like.
  • heteroaryl (on its own or in any combination, such as “heteroaryloxy”, or “heteroaryl alkyl”) - a 5-10 membered aromatic ring system in which one or more rings contain one or more heteroatoms selected from the group consisting of N, O or S, such as, but not limited, to pyrrole, pyrazole, furan, thiophene, quinoline, isoquinoline, quinazolinyl, pyridine, pyrimidine, oxazole, thiazole, thiadiazole, triazole, imidazole, or benzimidazole.
  • heterocyclic (on its own or in any combination, such as “heterocyclicalkyl”) - a saturated or partially unsaturated 4-10 membered ring system in which one or more rings contain one or more heteroatoms selected from the group consisting of N, O, or S; such as, but not limited to, pyrrolidine, piperidine, piperazine, morpholine, tetrahydropyran, or imidazolidine.
  • arylalkyl or heteroarylalkyl or “heterocyclicalkyl” is used herein to mean Ci-io alkyl, as defined above, attached to an aryl, heteroaryl or heterocyclic moiety, as also defined herein, unless otherwise indicated.
  • the compounds of Formula (I) may be obtained by applying synthetic procedures, some of which are illustrated in the Schemes below. The synthesis provided for in these Schemes is applicable for the producing compounds of Formula (I) having a variety of different R, Ri , and aryl groups which are reacted, employing optional substituents which are suitably protected, to achieve compatibility with the reactions outlined herein. Subsequent deprotection, in those cases, then affords compounds of the nature generally disclosed. Once the urea nucleus has been established, further compounds of these formulas may be prepared by applying standard techniques for functional group interconversion, well known in the art. While the schemes are shown with W and R 2 Q as phenyl this is merely for illustration purposes only.
  • the desired aniline 2-scheme-l can be synthesized by the reduction of the corresponding nitro if it is not available commercially. This reduction can be accomplished by a number of reducing agents such as hydrogen and catalytic
  • This aniline (2-scheme 1) can then be condensed with a commercially available isocyanate in an aprotic solvent such as DMF, DMSO or toluene.
  • the desired compound could synthesized by the protection of the carboxylic acid by conditions well known in art, such as diazome thane to form the methyl ester.
  • This compound could then be reduced by a number of reducing agents such as hydrogen and catalytic Palladium on carbon or tin chloride in a polar solvent such as DMF or ethyl acetate. Condensation with a phosgene equivalent such as di- or triphosgene in the presence of a base such as triethyl amine or bicarbonate would form the isocyanate 4-scheme-2.
  • This compound could then be reacted with the desired commercially available aniline.
  • the carboxylic acid could then be deprotected by conditions standard in the art, such as metal hydroxide in a polar solvent such as THF/water, then acidified with an acid such as HC1 to form 3, scheme 2.
  • R CH 2 CH 2 COOH, COOH, or CH COOH;
  • Protected R wherein R CH 2 CH COOMe, COOMe, or CH 2 COOMe a) CH 2 N 2 , ether b) H 2 ,5%Pd/C c) triphosgene, Et N d) PhNH 2 ,DMF e) LiOH, THF/H 2 O f) HCl/H 2 O
  • compositions of Formula (I) may be obtained in known manner, for example by treatment thereof with an appropriate amount of acid or base in the presence of a suitable solvent.
  • Another aspect of the present invention is the analogous process for producing a compound of Formula (I) which process comprises reacting a compound of the formula
  • R, Rj and m are as defined for Formula (I), with a compound of the formula:
  • Another aspect of the present invention is the alternative process for producing a compound of Formula (I) which process comprises reacting a compound of the formula:
  • Ri , m and R are as defined for formula (I); with a compound of the formula: NH 2 -(CR 13 R 14 ) V - R 20 ; wherein Ri 3, R14, v and R 2 Q are as defined in Formula (I) to yield a compound of Formula (I); and deprotecting the R group if necessary.
  • Ri , m and R are as defined for formula (I); with a compound of the formula: NH 2 -(CR 13 R 14 ) V - R 20 ; wherein Ri 3, R14, v and R 2 Q are as defined in Formula (I) to yield a compound of Formula (I); and deprotecting the R group if necessary.
  • the compounds of Formula (I), or a pharmaceutically acceptable salt thereof can be used in the manufacture of a medicament for the prophylactic or therapeutic treatment of any disease state in a human, or other mammal, which is exacerbated or caused by excessive or unregulated IL-8 cytokine production by such mammal's cell, such as but not limited to monocytes and/or macrophages, or other chemokines which bind to the IL-8 ⁇ or ⁇ receptor, also referred to as the type I or type II receptor.
  • the present invention provides a method of treating a chemokine mediated disease, wherein the chemokine is one which binds to an IL-8 ⁇ or ⁇ receptor and which method comprises administering an effective amount of a compound of Formula (I) or a pharmaceutically acceptable salt thereof.
  • the chemokines are IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2 or ENA-78.
  • the compounds of Formula (I) are administered in an amount sufficient to inhibit cytokine function, in particular IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2 or ENA-78, such that they are biologically regulated down to normal levels of physiological function, or in some case to subnormal levels, so as to ameliorate the disease state.
  • Abnormal levels of IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2 or ENA-78 for instance in the context of the present invention constitute: (i) levels of free IL-8 greater than or equal to 1 picogram per mL; (ii) any cell associated IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2 or ENA-78 above normal physiological levels; or (iii) the presence IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2 or ENA-78 above basal levels in cells or tissues in IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ NAP-2 or ENA-78 respectively, is produced.
  • interleukin-8 and rhinovirus may be found in articles such as: Turner,et al., Clin. Infect. Dis. (1998), 26(4), 840-846; Sanders, et al., J. Virol. (1998), 72(2), 934-942; Sethi, et al., Clin. Exp. Immunol. (1997), 110(3), 362-369; Zhu, et al., Am. J. Physiol. ( 1997), 273(4, Pt. 1), L814-L824; Terajima, et al., Am. J. Physiol. (1997), 273(4, Pt. 1), L749-L759; Grunberg, et al., Clin. Exp.
  • IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ or NAP-2 has the unique property of promoting neutrophil chemotaxis, enzyme release including but not limited to elastase release as well as superoxide production and activation.
  • the ⁇ -chemokines but particularly, GRO ⁇ , GRO ⁇ , GRO ⁇ or NAP-2, working through the IL-8 type I or II receptor can promote the neovascularization of tumors by promoting the directional growth of endothelial cells. Therefore, the inhibition of IL-8 induced chemotaxis or activation would lead to a direct reduction in the neutrophil infiltration.
  • the present invention also provides for a means of treating, in an acute setting, as well as preventing, in those individuals deemed susceptible to, CNS injuries by the chemokine receptor antagonist compounds of Formula (I).
  • CNS injuries as defined herein include both open or penetrating head trauma, such as by surgery, or a closed head trauma injury, such as by an injury to the head region. Also included within this definition is ischemic stroke, particularly to the brain area.
  • Ischemic stroke may be defined as a focal neurologic disorder that results from insufficient blood supply to a particular brain area, usually as a consequence of an embolus, thrombi, or local atheromatous closure of the blood vessel.
  • the role of inflammatory cytokines in this are has been emerging and the present invention provides a mean for the potential treatment of these injuries. Relatively little treatment, for an acute injury such as these has been available.
  • TNF- ⁇ is a cytokine with proinflammatory actions, including endothelial leukocyte adhesion molecule expression.
  • Leukocytes infiltrate into ischemic brain lesions and hence compounds which inhibit or decrease levels of TNF would be useful for treatment of ischemic brain injury. See Liu et al., Stoke, Vol. 25., No. 7, pp 1481-88 (1994) whose disclosure is incorporated herein by reference.
  • the compounds of Formula (I) are administered in an amount sufficient to inhibit IL-8, binding to the IL-8 alpha or beta receptors, from binding to these receptors, such as evidenced by a reduction in neutrophil chemotaxis and activation.
  • the compounds of Formula (I) are inhibitors of IL-8 binding is based upon the effects of the compounds of Formulas (I) in the in vitro receptor binding assays which are described herein.
  • the compounds of Formula (I) have been shown, in some instances, to be dual inhibitors of both recombinant type I and type II IL-8 receptors.
  • the compounds are inhibitors of only one receptor, more preferably Type II.
  • IL-8 mediated disease or disease state refers to any and all disease states in which IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2 or ENA-78 plays a role, either by production of IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2 or ENA-78 themselves, or by IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2 or ENA-78 causing another monokine to be released, such as but not limited to IL-1, IL-6 or TNF.
  • chemokine mediated disease or disease state refers to any and all disease states in which a chemokine which binds to an IL-8 ⁇ or ⁇ receptor plays a role, such as but not limited IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2 or ENA-78.
  • IL-8 plays a role, either by production of IL-8 itself, or by IL-8 causing another monokine to be released, such as but not limited to IL-1, IL-6 or TNF.
  • Such diseases include but are not limited to psoriasis, atopic dermatitis, arthritis, asthma, chronic obstructive pulmonary disease, adult respiratory distress syndrome, inflammatory bowel disease, Crohn's disease, ulcerative colitis, stroke, septic shock, endotoxic shock, gram negative sepsis, toxic shock syndrome, cardiac and renal reperfusion injury, glomerulonephritis, thrombosis, graft vs.
  • hematopoietic stem cells release and diseases caused by respiratory viruses, including but not limited to rhinovirus and influenza virus, herpes viruses, including but not limited to herpes simplex I and II, and hepatitis viruses, including but not limited to Hepatitis B and Hepatitis C virus.
  • respiratory viruses including but not limited to rhinovirus and influenza virus, herpes viruses, including but not limited to herpes simplex I and II, and hepatitis viruses, including but not limited to Hepatitis B and Hepatitis C virus.
  • cytokine refers to any secreted polypeptide that affects the functions of cells and is a molecule which modulates interactions between cells in the immune, inflammatory or hematopoietic response.
  • a cytokine includes, but is not limited to, monokines and lymphokines, regardless of which cells produce them.
  • a monokine is generally referred to as being produced and secreted by a mononuclear cell, such as a macrophage and/or monocyte.
  • Lymphokines are generally referred to as being produced by lymphocyte cells.
  • cytokines include, but are not limited to, Interleukin- 1 (IL-1), Interleukin-6 (IL-6), Interleukin-8 (IL-8), Tumor Necrosis Factor-alpha (TNF- ⁇ ) and Tumor Necrosis Factor beta (TNF- ⁇ ).
  • chemokine refers to any secreted polypeptide that affects the functions of cells and is a molecule which modulates interactions between cells in the immune, inflammatory or hematopoietic response, similar to the term “cytokine” above.
  • a chemokine is primarily secreted through cell transmembranes and causes chemotaxis and activation of specific white blood cells and leukocytes, neutrophils, monocytes, macrophages, T-cells, B-cells, endothelial cells and smooth muscle cells.
  • chemokines include, but are not limited to, IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ , NAP-2, ENA-78, IP-10, MlP-l ⁇ , MlP- ⁇ PF4, and MCP 1, 2, and 3.
  • a pharmaceutical composition comprising an effective, non- toxic amount of a compound of Formula (I) and a pharmaceutically acceptable carrier or diluent.
  • Compounds of Formula (I), pharmaceutically acceptable salts thereof and pharmaceutical compositions incorporating such may conveniently be administered by any of the routes conventionally used for drug administration, for instance, orally, topically, parenterally or by inhalation.
  • the compounds of Formula (I) may be administered in conventional dosage forms prepared by combining a compound of Formula (I) with standard pharmaceutical carriers according to conventional procedures.
  • the compounds of Formula (I) may also be administered in conventional dosages in combination with a known, second therapeutically active compound. These procedures may involve mixing, granulating and compressing or dissolving the ingredients as appropriate to the desired preparation.
  • the form and character of the pharmaceutically acceptable character or diluent is dictated by the amount of active ingredient with which it is to be combined, the route of administration and other well-known variables.
  • the carrier(s) must be "acceptable” in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
  • the pharmaceutical carrier employed may be, for example, either a solid or liquid. Exemplary of solid carriers are lactose, terra alba, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, stearic acid and the like. Exemplary of liquid carriers are syrup, peanut oil, olive oil, water and the like.
  • the carrier or diluent may include time delay material well known to the art, such as glyceryl mono-stearate or glyceryl distearate alone or with a wax.
  • the preparation can be tableted, placed in a hard gelatin capsule in powder or pellet form or in the form of a troche or lozenge.
  • the amount of solid carrier will vary widely but preferably will be from about 25mg. to about lg.
  • the preparation will be in the form of a syrup, emulsion, soft gelatin capsule, sterile injectable liquid such as an ampule or nonaqueous liquid suspension.
  • Compounds of Formula (I) may be administered topically, that is by non- systemic administration. This includes the application of a compound of Formula (I) externally to the epidermis or the buccal cavity and the instillation of such a compound into the ear, eye and nose, such that the compound does not significantly enter the blood stream.
  • systemic administration refers to oral, intravenous, intraperitoneal and intramuscular administration.
  • Formulations suitable for topical administration include liquid or semi-liquid preparations suitable for penetration through the skin to the site of inflammation such as liniments, lotions, creams, ointments or pastes, and drops suitable for administration to the eye, ear or nose.
  • the active ingredient may comprise, for topical administration, from 0.001% to 10% w/w, for instance from 1% to 2% by weight of the Formulation. It may however comprise as much as 10% w/w but preferably will comprise less than 5% w/w, more preferably from 0.1% to 1% w/w of the Formulation.
  • Lotions according to the present invention include those suitable for application to the skin or eye.
  • An eye lotion may comprise a sterile aqueous solution optionally containing a bactericide and may be prepared by methods similar to those for the preparation of drops.
  • Lotions or liniments for application to the skin may also include an agent to hasten drying and to cool the skin, such as an alcohol or acetone, and/or a moisturizer such as glycerol or an oil such as castor oil or arachis oil.
  • Creams, ointments or pastes according to the present invention are semi-solid formulations of the active ingredient for external application.
  • the base may comprise hydrocarbons such as hard, soft or liquid paraffin, glycerol, beeswax, a metallic soap; a mucilage; an oil of natural origin such as almond, corn, arachis, castor or olive oil; wool fat or its derivatives or a fatty acid such as steric or oleic acid together with an alcohol such as propylene glycol or a macrogel.
  • hydrocarbons such as hard, soft or liquid paraffin, glycerol, beeswax, a metallic soap
  • a mucilage an oil of natural origin such as almond, corn, arachis, castor or olive oil
  • wool fat or its derivatives or a fatty acid such as steric or oleic acid together with an alcohol such as propylene glycol or a macrogel.
  • the formulation may incorporate any suitable surface active agent such as an anionic, cationic or non-ionic surfactant such as a sorbitan ester or a polyoxyethylene derivative thereof.
  • suitable surface active agent such as an anionic, cationic or non-ionic surfactant such as a sorbitan ester or a polyoxyethylene derivative thereof.
  • Suspending agents such as natural gums, cellulose derivatives or inorganic materials such as silicaceous silicas, and other ingredients such as lanolin, may also be included.
  • Drops according to the present invention may comprise sterile aqueous or oily solutions or suspensions and may be prepared by dissolving the active ingredient in a suitable aqueous solution of a bactericidal and/or fungicidal agent and/or any other suitable preservative, and preferably including a surface active agent.
  • the resulting solution may then be clarified by filtration, transferred to a suitable container which is then sealed and sterilized by autoclaving or maintaining at 98-100 °C. for half an hour.
  • the solution may be sterilized by filtration and transferred to the container by an aseptic technique.
  • bactericidal and fungicidal agents suitable for inclusion in the drops are phenylmercuric nitrate or acetate (0.002%), benzalkonium chloride (0.01%) and chlorhexidine acetate (0.01%).
  • Suitable solvents for the preparation of an oily solution include glycerol, diluted alcohol and propylene glycol.
  • Compounds of formula (I) may be administered parenterally, that is by intravenous, intramuscular, subcutaneous intranasal, intrarectal, intravaginal or intraperitoneal administration. The subcutaneous and intramuscular forms of parenteral administration are generally preferred. Appropriate dosage forms for such administration may be prepared by conventional techniques.
  • Compounds of Formula (I) may also be administered by inhalation, that is by intranasal and oral inhalation administration.
  • Appropriate dosage forms for such administration such as an aerosol formulation or a metered dose inhaler, may be prepared by conventional techniques.
  • the daily oral dosage regimen will preferably be from about 0.01 to about 80 mg/kg of total body weight.
  • the daily parenteral dosage regimen about 0.001 to about 80 mg/kg of total body weight.
  • the daily topical dosage regimen will preferably be from 0.1 mg to 150 mg, administered one to four, preferably two or three times daily.
  • the daily inhalation dosage regimen will preferably be from about 0.01 mg/kg to about 1 mg/kg per day.
  • the optimal quantity and spacing of individual dosages of a compound of Formula (I) or a pharmaceutically acceptable salt thereof will be determined by the nature and extent of the condition being treated, the form, route and site of administration, and the particular patient being treated, and that such optimums can be determined by conventional techniques. It will also be appreciated by one of skill in the art that the optimal course of treatment, i.e., the number of doses of a compound of Formula (I) or a pharmaceutically acceptable salt thereof given per day for a defined number of days, can be ascertained by those skilled in the art using conventional course of treatment determination tests.
  • BIOLOGICAL EXAMPLES The IL-8, and GRO- ⁇ chemokine inhibitory effects of compounds of the present invention were determined by the following in vitro assay: Receptor Binding Assays:
  • 125j IL_ 8 human recombinant was obtained from Amersham Corp., Arlington Heights, IL, with specific activity 2000 Ci/mmol. GRO- ⁇ was obtained from NEN- New England Nuclear. All other chemicals were of analytical grade. High levels of recombinant human IL-8 type ⁇ and ⁇ receptors were individually expressed in Chinese hamster ovary cells as described previously (Holmes, et al., Science, 1991, 253, 1278). The Chinese hamster ovary membranes were homogenized according to a previously described protocol (Haour, et al., J Biol Chem., 249 pp 2195-2205 (1974)).
  • homogenization buffer was changed to lOmM Tris-HCL, lmM MgS04, 0.5mM EDTA (ethylene-diaminetetra- acetic acid), lmMPMSF ( ⁇ -toluenesulphonyl fluoride), 0.5 mg L Leupeptin, pH 7.5.
  • Membrane protein concentration was determined using Pierce Co. micro-assay kit using bovine serum albumin as a standard. All assays were performed in a 96-well micro plate format.
  • Each reaction mixture contained 125 L_ (0.25 nM) or 125j Gro- ⁇ and 0.5 ⁇ g/mL of IL-8R ⁇ or 1.0 ⁇ g/mL of IL-8R ⁇ membranes in 20 mM Bis- Trispropane and 0.4 mM Tris HC1 buffers, pH 8.0, containing 1.2 mM MgSO4, 0.1 mM EDTA, 25 mM NaCl and 0.03% CHAPS.
  • drug or compound of interest was added which had been pre-dissolved in DMSO so as to reach a final concentration of between O.OlnM and 100 uM.
  • the assay was initiated by addition of 125J_IL_ 8 .
  • the recombinant IL-8 R ⁇ or Type I, receptor is also referred to herein as the non- permissive receptor and the recombinant IL-8 R ⁇ or Type II, receptor is referred to as the permissive receptor. All of the exemplified compounds of Formulas (I) noted herein in the
  • the in vitro inhibitory properties of these compounds are determined in the neutrophil chemotaxis assay as described in Current Protocols in Immunology, vol. I, Suppl 1, Unit 6.12.3., whose disclosure is incorporated herein by reference in its entirety.
  • Neutrophils where isolated from human blood as described in Current Protocols in Immunology Vol. I, Suppl 1 Unit 7.23.1, whose disclosure is incorporated herein by reference in its entirety.
  • the chemoattractants IL-8, GRO ⁇ , GRO ⁇ , GRO ⁇ and NAP-2 are placed in the bottom chamber of a 48 multiwell chamber (Neuro Probe, Cabin John, MD) at a concentration between 0.1 and 100 nM. The two chambers are separated by a 5um polycarbonate filter.
  • the compounds of this invention are tested for their ability to prevent Elastase release from human neutrophils.
  • Neutrophils are isolated from human blood as described in Current Protocols in Immunology Vol. I, Suppl 1 Unit 7.23.1.
  • PMNs 0.88 x 10 6 cells suspended in Ringer's Solution (NaCl 118, KC1 4.56,
  • This assay provides for examination of the expression of tumor necrosis factor mRNA in specific brain regions, which follow experimentally, induced lateral fluid- percussion traumatic brain injury (TBI) in rats. Since TNF- ⁇ is able to induce nerve growth factor (NGF) and stimulate the release of other cytokines from activated astrocytes, this post-traumatic alteration in gene expression of TNF- ⁇ plays an important role in both the acute and regenerative response to CNS trauma.
  • a suitable assay may be found in WO 97/35856 or WO 97/49286 whose disclosures are incorporated herein by reference.
  • This assay characterizes the regional expression of interleukin-l ⁇ (IL-l ⁇ ) mRNA in specific brain regions following experimental lateral fluid-percussion traumatic brain injury (TBI) in rats. Results from these assays indicate that following TBI, the temporal expression of IL-l ⁇ mRNA is regionally stimulated in specific brain regions. These regional changes in cytokines, such as IL-l ⁇ play a role in the post- traumatic pathologic or regenerative sequelae of brain injury.
  • TBI lateral fluid-percussion traumatic brain injury
  • In vivo - Athereoschlerosis assay In vivo models for measuring atherosclerosis in mice is based on the assay of Paigen et al with small modifications as described below. See Paigen B, Morrow A, Holmes PA, Mitchell D, Williams RA. Quantitative assessment of atherosclerotic lesions in mice. Atherosclerosis 68: 231-240 (1987); and Groot PHE, van Vlijmen BJM, Benson GM, Hofker MH, Schiffelers R, Vidgeon-Hart M, Havekes LM. Quantitative assessment of aortic atherosclerosis in APOE*3 Leiden transgenic mice and its relationship to serum cholesterol exposure. Arterioscler Thromb Vase Biol. 16: 926-933 (1996). Sectioning and staining of the aortic sinus
  • Cross-sections of the aortic root are taken as has been described previously (1,2). Briefly, the hearts are bisected just below the level of the atria and the base of the heart plus aortic root are taken for analysis. After equilibrating the tissue in OCT compound overnight the hearts are immersed in OCT compound on a cryostat chuck (Bright Instrument Company Ltd., UK) with the aorta facing the chuck. The tissue is frozen by surrounding the chuck with dry ice. The hearts are then sectioned perpendicular to the axis of the aorta, starting within the heart and working in the direction of the aorta.
  • Ten alternate sections of the aortic root are imaged using an Olympus BH-2 microscope equipped with an 4x objective and a video camera (Hitachi, HV-C10). Twenty-four bit colour images are acquired and analyzed using a PC (Datacell Pentium P5-133, Datacell, Berks, U.K.) fitted with a framegrabbing board (Snapper, Active Imaging Ltd, Berks, U.K.) and running Optimas software (version 5.1, Optimas Corp., WA, U.S.A.). The images are captured under identical lighting, microscope, camera and PC conditions. Quantification of the atherosclerotic lesion areas is performed by drawing around the lesions by hand using the Optimas software. Colour thresholds are set that quantify the areas that are stained red within the lesions. Absolute values for the cross-sectional areas of the lesions and the areas stained red are obtained by calibrating the software using an image of the grid on a haemocytometer slide.

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Abstract

La présente invention concerne de nouvelles phénylurées utiles dans le traitement de pathologies qui ont pour médiateur une chimiokine, plus précisément l'interleukine 8 (IL-8).
PCT/US2000/016510 1999-06-16 2000-06-15 Antagonistes du recepteur il-8 WO2000076515A1 (fr)

Priority Applications (12)

Application Number Priority Date Filing Date Title
NZ515609A NZ515609A (en) 1999-06-16 2000-06-15 IL-8 receptor antagonists
JP2001502848A JP2003501470A (ja) 1999-06-16 2000-06-15 Ｉｌ−８受容体アンタゴニスト
KR1020017016141A KR20020012275A (ko) 1999-06-16 2000-06-15 Ｉｌ-8 수용체 길항제
CA002377391A CA2377391A1 (fr) 1999-06-16 2000-06-15 Antagonistes du recepteur il-8
PL00352222A PL352222A1 (en) 1999-06-16 2000-06-15 Antagonists of the il-8 receptor
BR0011567-3A BR0011567A (pt) 1999-06-16 2000-06-15 Antagonistas de receptor il-8
IL14651500A IL146515A0 (en) 1999-06-16 2000-06-15 Il-8 receptor antagonists
AU58750/00A AU767000B2 (en) 1999-06-16 2000-06-15 IL-8 receptor antagonists
EP00944689A EP1185271A4 (fr) 1999-06-16 2000-06-15 Antagonistes du recepteur il-8
MXPA01013089A MXPA01013089A (es) 1999-06-16 2000-06-15 Antagonistas de receptor de interleucina-8.
NO20016102A NO20016102L (no) 1999-06-16 2001-12-14 IL-8 reseptorantagonister
AU2004200500A AU2004200500A1 (en) 1999-06-16 2004-02-11 IL-8 receptor antagonists

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13967399P 1999-06-16 1999-06-16
US60/139,673 1999-06-16

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10018039 A-371-Of-International 2002-03-15
US10/463,270 Continuation US20030225125A1 (en) 1999-06-16 2003-06-17 IL-8 receptor antagonists

Publications (1)

Publication Number Publication Date
WO2000076515A1 true WO2000076515A1 (fr) 2000-12-21

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/016510 WO2000076515A1 (fr) 1999-06-16 2000-06-15 Antagonistes du recepteur il-8

Country Status (20)

Country Link
EP (1) EP1185271A4 (fr)
JP (1) JP2003501470A (fr)
KR (1) KR20020012275A (fr)
CN (1) CN1356902A (fr)
AR (1) AR024349A1 (fr)
AU (1) AU767000B2 (fr)
BR (1) BR0011567A (fr)
CA (1) CA2377391A1 (fr)
CO (1) CO5190709A1 (fr)
CZ (1) CZ20014491A3 (fr)
HU (1) HUP0202004A3 (fr)
IL (1) IL146515A0 (fr)
MX (1) MXPA01013089A (fr)
NO (1) NO20016102L (fr)
NZ (1) NZ515609A (fr)
PL (1) PL352222A1 (fr)
TR (1) TR200103608T2 (fr)
UY (1) UY26206A1 (fr)
WO (1) WO2000076515A1 (fr)
ZA (1) ZA200110205B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008110771A2 (fr) * 2007-03-09 2008-09-18 Ucl Business Plc Composition

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0344425A2 (fr) * 1988-03-30 1989-12-06 Warner-Lambert Company N-[[(phényl 2,6 disubstitué)]N'- arylalkyl] urées comme agents antihypercholesterolemiques et antiatherosclérotiques
US5780483A (en) * 1995-02-17 1998-07-14 Smithkline Beecham Corporation IL-8 receptor antagonists

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2294064A1 (fr) * 1997-07-29 1999-02-11 Smithkline Beecham Corporation Antagonistes du recepteur il-8

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0344425A2 (fr) * 1988-03-30 1989-12-06 Warner-Lambert Company N-[[(phényl 2,6 disubstitué)]N'- arylalkyl] urées comme agents antihypercholesterolemiques et antiatherosclérotiques
US5780483A (en) * 1995-02-17 1998-07-14 Smithkline Beecham Corporation IL-8 receptor antagonists

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1185271A4 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008110771A2 (fr) * 2007-03-09 2008-09-18 Ucl Business Plc Composition
WO2008110771A3 (fr) * 2007-03-09 2008-12-18 Ucl Business Plc Composition

Also Published As

Publication number Publication date
AU767000B2 (en) 2003-10-30
MXPA01013089A (es) 2002-06-04
AR024349A1 (es) 2002-10-02
IL146515A0 (en) 2002-07-25
NO20016102L (no) 2002-02-12
EP1185271A4 (fr) 2004-02-04
CA2377391A1 (fr) 2000-12-21
ZA200110205B (en) 2002-08-12
TR200103608T2 (tr) 2002-06-21
AU5875000A (en) 2001-01-02
NO20016102D0 (no) 2001-12-14
BR0011567A (pt) 2002-03-05
PL352222A1 (en) 2003-08-11
UY26206A1 (es) 2000-12-29
CN1356902A (zh) 2002-07-03
KR20020012275A (ko) 2002-02-15
CZ20014491A3 (cs) 2002-07-17
EP1185271A1 (fr) 2002-03-13
NZ515609A (en) 2003-11-28
CO5190709A1 (es) 2002-08-29
HUP0202004A3 (en) 2003-03-28
JP2003501470A (ja) 2003-01-14
HUP0202004A2 (en) 2002-10-28

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