WO2000065095A3 - Methods and compositions for performing array based hybridization assays - Google Patents

Methods and compositions for performing array based hybridization assays Download PDF

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Publication number
WO2000065095A3
WO2000065095A3 PCT/US2000/010894 US0010894W WO0065095A3 WO 2000065095 A3 WO2000065095 A3 WO 2000065095A3 US 0010894 W US0010894 W US 0010894W WO 0065095 A3 WO0065095 A3 WO 0065095A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
methods
hybridization assays
compositions
based hybridization
Prior art date
Application number
PCT/US2000/010894
Other languages
French (fr)
Other versions
WO2000065095A2 (en
Inventor
Alex Chenchik
Alexander Munishkin
Original Assignee
Clontech Lab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clontech Lab Inc filed Critical Clontech Lab Inc
Priority to EP00931942A priority Critical patent/EP1196633A2/en
Publication of WO2000065095A2 publication Critical patent/WO2000065095A2/en
Publication of WO2000065095A3 publication Critical patent/WO2000065095A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods for performing array-based hybridization assays, as well as sets of control target nucleic acids for use in the subject methods and kits comprising the same, are provided. In the subject methods, test and control sets of target nucleic acids are contacted with an array(s), where the test and control sets of target nucleic acids may or may not be labeled differentially. The subject control sets of nucleic acids contain a plurality of nucleic acids, with at least one nucleic acid of the set binding selectively to at least a subset of the probe nucleic acids present on an array.
PCT/US2000/010894 1999-04-23 2000-04-20 Methods and compositions for performing array based hybridization assays WO2000065095A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP00931942A EP1196633A2 (en) 1999-04-23 2000-04-20 Methods and compositions for performing array based hybridization assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29836199A 1999-04-23 1999-04-23
US09/298,361 1999-04-23

Publications (2)

Publication Number Publication Date
WO2000065095A2 WO2000065095A2 (en) 2000-11-02
WO2000065095A3 true WO2000065095A3 (en) 2002-02-21

Family

ID=23150172

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/010894 WO2000065095A2 (en) 1999-04-23 2000-04-20 Methods and compositions for performing array based hybridization assays

Country Status (2)

Country Link
EP (1) EP1196633A2 (en)
WO (1) WO2000065095A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001017166A (en) * 1999-07-02 2001-01-23 Fuji Photo Film Co Ltd Dna chip, verifying kit of dna chip and verification of dna chip
EP1598432A3 (en) 2000-07-31 2006-06-07 Agilent Technologies, Inc. Array based methods for sythesizing nucleic acid mixtures
AU2002226984A1 (en) * 2000-11-27 2002-06-03 Astrazeneca Ab Method for studying the effects of commensal microflora on mammalian intestine and treatments of gastrointestinal-associated disease based thereon
BE1014949A3 (en) * 2001-08-14 2004-07-06 Probiox Oxidative stress detection method and kit for its implementation.
AU2002361146A1 (en) * 2001-12-21 2003-07-09 Pamgene B.V. Normalisation of microarray data based on hybridisation with an internal reference
US7977119B2 (en) 2004-12-08 2011-07-12 Agilent Technologies, Inc. Chemical arrays and methods of using the same

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997010365A1 (en) * 1995-09-15 1997-03-20 Affymax Technologies N.V. Expression monitoring by hybridization to high density oligonucleotide arrays
WO1998053103A1 (en) * 1997-05-21 1998-11-26 Clontech Laboratories, Inc. Nucleic acid arrays
WO1998056954A1 (en) * 1997-06-13 1998-12-17 Affymetrix, Inc. Method to detect gene polymorphisms and monitor allelic expression employing a probe array

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997010365A1 (en) * 1995-09-15 1997-03-20 Affymax Technologies N.V. Expression monitoring by hybridization to high density oligonucleotide arrays
WO1998053103A1 (en) * 1997-05-21 1998-11-26 Clontech Laboratories, Inc. Nucleic acid arrays
WO1998056954A1 (en) * 1997-06-13 1998-12-17 Affymetrix, Inc. Method to detect gene polymorphisms and monitor allelic expression employing a probe array

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WANG S ET AL: "Internal cRNA standards for quantitative northern analysis", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 14, no. 6, 1993, pages 935 - 936,938,940-941, XP002133186, ISSN: 0736-6205 *

Also Published As

Publication number Publication date
EP1196633A2 (en) 2002-04-17
WO2000065095A2 (en) 2000-11-02

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