WO2000061727A3 - Production of pancreatic procarboxy-peptidase b, isoforms and muteins thereof, and their use - Google Patents

Production of pancreatic procarboxy-peptidase b, isoforms and muteins thereof, and their use Download PDF

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Publication number
WO2000061727A3
WO2000061727A3 PCT/EP2000/002571 EP0002571W WO0061727A3 WO 2000061727 A3 WO2000061727 A3 WO 2000061727A3 EP 0002571 W EP0002571 W EP 0002571W WO 0061727 A3 WO0061727 A3 WO 0061727A3
Authority
WO
WIPO (PCT)
Prior art keywords
peptidase
production
carboxy
procarboxy
pancreatic
Prior art date
Application number
PCT/EP2000/002571
Other languages
German (de)
French (fr)
Other versions
WO2000061727A2 (en
Inventor
Paul Habermann
Original Assignee
Aventis Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE50013563T priority Critical patent/DE50013563D1/en
Priority to NZ514657A priority patent/NZ514657A/en
Priority to JP2000611652A priority patent/JP2002541793A/en
Priority to IL14564600A priority patent/IL145646A0/en
Priority to KR1020017012888A priority patent/KR100714116B1/en
Priority to EP00926751A priority patent/EP1169461B1/en
Priority to CA2369973A priority patent/CA2369973C/en
Priority to BRPI0009636-9A priority patent/BR0009636B1/en
Application filed by Aventis Pharma Gmbh filed Critical Aventis Pharma Gmbh
Priority to AU45394/00A priority patent/AU777408B2/en
Publication of WO2000061727A2 publication Critical patent/WO2000061727A2/en
Publication of WO2000061727A3 publication Critical patent/WO2000061727A3/en
Priority to IL145646A priority patent/IL145646A/en
Priority to NO20014815A priority patent/NO326247B1/en
Priority to HK02106158.3A priority patent/HK1044566B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a method for producing pancreatic carboxy-peptidase B or an isoform or a mutein of carboxy-peptidase B, whereby: (a) a natural or unnatural precursor form of carboxy-peptidase B is expressed in a secretory manner in a microorganism; (b) the precursor form expressed in a secretory manner is purified, and; (c) the purified precursor form is converted into the active carboxy-peptidase B or into the isoform or the mutein thereof by means of an enzymatic treatment. The invention also relates to a nucleic acid construct, to its use in the aforementioned method, and to the production thereof, as well as to a host cell, the production thereof and its use in the method for producing pancreatic procarboxy-peptidase B or an isoform or a mutein thereof.
PCT/EP2000/002571 1999-04-09 2000-03-23 Production of pancreatic procarboxy-peptidase b, isoforms and muteins thereof, and their use WO2000061727A2 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
CA2369973A CA2369973C (en) 1999-04-09 2000-03-23 Preparation of pancreatic procarboxypeptidase b, isoforms and muteins thereof and their use
JP2000611652A JP2002541793A (en) 1999-04-09 2000-03-23 Production of pancreatic procarboxypeptidase B, its isoforms and muteins and their use
IL14564600A IL145646A0 (en) 1999-04-09 2000-03-23 Production of pancreatic procarboxy-peptidase b, isoforms and muteins thereof, and their use
KR1020017012888A KR100714116B1 (en) 1999-04-09 2000-03-23 Production of insulin with pancreatic procarboxypeptidase B
EP00926751A EP1169461B1 (en) 1999-04-09 2000-03-23 Use of pancreatic procarboxypeptidase B for the production of insulin
DE50013563T DE50013563D1 (en) 1999-04-09 2000-03-23 Use of pancreatic procarboxypeptidase B for the production of insulin
BRPI0009636-9A BR0009636B1 (en) 1999-04-09 2000-03-23 process for the preparation of mature insulin or a derivative of mature insulin.
NZ514657A NZ514657A (en) 1999-04-09 2000-03-23 Production of pancreatic procarboxy-peptidase B, isoforms and muteins thereof, and their use
AU45394/00A AU777408B2 (en) 1999-04-09 2000-03-23 Production of pancreatic procarboxy-peptidase B, isoforms and muteins thereof, and their use
IL145646A IL145646A (en) 1999-04-09 2001-09-25 Production of pancreatic procarboxy-peptidase b, isoforms and muteins thereof
NO20014815A NO326247B1 (en) 1999-04-09 2001-10-03 Process for producing mature insulin or a mature insulin derivative.
HK02106158.3A HK1044566B (en) 1999-04-09 2002-08-22 Production of pancreatic procarboxy-peptidase b, isoforms and muteins thereof, and their use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19915938A DE19915938A1 (en) 1999-04-09 1999-04-09 Production of pancreatic procarboxypeptidase B, isoforms and muteins thereof and their use
DE19915938.6 1999-04-09

Publications (2)

Publication Number Publication Date
WO2000061727A2 WO2000061727A2 (en) 2000-10-19
WO2000061727A3 true WO2000061727A3 (en) 2001-02-01

Family

ID=7903951

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/002571 WO2000061727A2 (en) 1999-04-09 2000-03-23 Production of pancreatic procarboxy-peptidase b, isoforms and muteins thereof, and their use

Country Status (17)

Country Link
US (1) US6531294B1 (en)
EP (1) EP1169461B1 (en)
JP (1) JP2002541793A (en)
KR (1) KR100714116B1 (en)
CN (1) CN100362105C (en)
AT (1) ATE341630T1 (en)
AU (1) AU777408B2 (en)
BR (1) BR0009636B1 (en)
CA (1) CA2369973C (en)
DE (2) DE19915938A1 (en)
ES (1) ES2270827T3 (en)
HK (1) HK1044566B (en)
IL (2) IL145646A0 (en)
NO (1) NO326247B1 (en)
NZ (1) NZ514657A (en)
WO (1) WO2000061727A2 (en)
ZA (1) ZA200108241B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7291587B2 (en) 2001-11-09 2007-11-06 Novo Nordisk Healthcare A/G Pharmaceutical composition comprising factor VII polypeptides and TAFI polypeptides
EP1538203B1 (en) * 2003-12-05 2010-01-20 Roche Diagnostics GmbH Recombinantly expressed carboxypeptidase B and purification thereof
JP4411192B2 (en) * 2003-12-05 2010-02-10 エフ．ホフマン−ラ ロシュ アーゲー Recombinantly expressed carboxypeptidase B and purification thereof
DK1794294T3 (en) * 2004-09-27 2011-10-31 Sanofi Aventis Deutschland Recombinant carboxypeptidase B
JP5404411B2 (en) * 2006-11-22 2014-01-29 ノボ・ノルデイスク・エー／エス Process for producing activated carboxypeptidase
CN101058805B (en) * 2007-03-21 2011-09-07 北京贯虹科技有限公司 Method of producing mutation procarboxypeptidase B and mutation carboxypeptidase B

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995014096A1 (en) * 1993-11-16 1995-05-26 Eli Lilly And Company Dna sequences encoding porcine pancreatic carboxypeptidase b
WO1996002637A1 (en) * 1994-07-15 1996-02-01 Eli Lilly And Company Method for extra-cellular expression of protein
WO1996023064A1 (en) * 1995-01-25 1996-08-01 Bio-Technology General Corp. Production of enzymatically active recombinant carboxypeptidase b

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2061797T3 (en) * 1988-06-23 1994-12-16 Hoechst Ag MINI-PROINSULIN, ITS PREPARATION AND EMPLOYMENT.
IL95495A (en) 1989-08-29 1996-10-16 Hoechst Ag Fusion proteins their preparation and use
HU214828B (en) * 1989-10-31 1998-06-29 Schering Corp. Method for producing e. coli strains being capasle of secreting heterologous proteins into the culture medium and for producing heterologous proteins
DE4009268A1 (en) 1990-03-22 1991-09-26 Consortium Elektrochem Ind SECRETION OF HIRUDIN DERIVATIVES
FI103805B1 (en) * 1990-09-05 1999-09-30 Hoechst Ag Method for hydrolysis of preproinsulins
DE4136389A1 (en) 1991-03-18 1992-10-08 Hoechst Ag PROCESS FOR THE PRODUCTION OF GLUTARYLACYLASE IN LARGE QUANTITIES

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995014096A1 (en) * 1993-11-16 1995-05-26 Eli Lilly And Company Dna sequences encoding porcine pancreatic carboxypeptidase b
WO1996002637A1 (en) * 1994-07-15 1996-02-01 Eli Lilly And Company Method for extra-cellular expression of protein
WO1996023064A1 (en) * 1995-01-25 1996-08-01 Bio-Technology General Corp. Production of enzymatically active recombinant carboxypeptidase b

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HOCULI E ET AL: "GENETIC APPROACH TO FACILITATE PURIFICATION OF RECOMBINANT PROTEINS WITH A NOVEL METAL CHELATE ADSORBENT", BIO/TECHNOLOGY,US,NATURE PUBLISHING CO. NEW YORK, November 1988 (1988-11-01), pages 1321 - 1325, XP002916185, ISSN: 0733-222X *
REVERTER DAVID ET AL: "Overexpression of human procarboxypeptidase A2 in Pichia pastoris and detailed characterization of its activation pathway.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 6, 6 February 1998 (1998-02-06), pages 3535 - 3541, XP002148710, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
CN100362105C (en) 2008-01-16
DE50013563D1 (en) 2006-11-16
NZ514657A (en) 2003-11-28
CA2369973C (en) 2011-09-27
US6531294B1 (en) 2003-03-11
EP1169461B1 (en) 2006-10-04
EP1169461A2 (en) 2002-01-09
ES2270827T3 (en) 2007-04-16
AU4539400A (en) 2000-11-14
AU777408B2 (en) 2004-10-14
HK1044566B (en) 2008-08-01
ZA200108241B (en) 2002-11-27
BR0009636B1 (en) 2010-11-16
BR0009636A (en) 2002-01-08
IL145646A (en) 2008-03-20
CN1346407A (en) 2002-04-24
NO20014815L (en) 2001-11-20
NO20014815D0 (en) 2001-10-03
DE19915938A1 (en) 2000-10-19
HK1044566A1 (en) 2002-10-25
KR20010109348A (en) 2001-12-08
WO2000061727A2 (en) 2000-10-19
CA2369973A1 (en) 2000-10-19
IL145646A0 (en) 2002-06-30
NO326247B1 (en) 2008-10-27
KR100714116B1 (en) 2007-05-07
ATE341630T1 (en) 2006-10-15
JP2002541793A (en) 2002-12-10

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