WO2000057173A9 - Amortisseur de pulsations - Google Patents
Amortisseur de pulsationsInfo
- Publication number
- WO2000057173A9 WO2000057173A9 PCT/US2000/006809 US0006809W WO0057173A9 WO 2000057173 A9 WO2000057173 A9 WO 2000057173A9 US 0006809 W US0006809 W US 0006809W WO 0057173 A9 WO0057173 A9 WO 0057173A9
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- assembly
- cartridge
- tubing
- inches
- inlet
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B11/00—Equalisation of pulses, e.g. by use of air vessels; Counteracting cavitation
- F04B11/0008—Equalisation of pulses, e.g. by use of air vessels; Counteracting cavitation using accumulators
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B19/00—Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B1/00 - F04B17/00
- F04B19/006—Micropumps
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B43/00—Machines, pumps, or pumping installations having flexible working members
- F04B43/02—Machines, pumps, or pumping installations having flexible working members having plate-like flexible members, e.g. diaphragms
- F04B43/04—Pumps having electric drive
- F04B43/043—Micropumps
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B9/00—Piston machines or pumps characterised by the driving or driven means to or from their working members
- F04B9/02—Piston machines or pumps characterised by the driving or driven means to or from their working members the means being mechanical
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
- G01N2030/328—Control of physical parameters of the fluid carrier of pressure or speed valves, e.g. check valves of pumps
Definitions
- the present invention relates generally to microfluidic devices for performing analytical testing and, in particular, to a fluidic driver system for use with microfluidic cartridges.
- Microfluidic devices have recently become popular for performing analytical testing. Using tools developed by the semiconductor industry to miniaturize electronics, it has become possible to fabricate intricate fluid systems which can be inexpensively mass produced. Systems have been developed to perform a variety of analytical techniques for the acquisition of information for the medical field.
- FFF field-flow fractionation
- SPLITT Split Flow Thin Cell
- Still another method used for assaying fluids involves application of electrical fields to a microfluidic system for providing capillary electrophoresis to separate materials in a flow channel. Examples of this process are taught in U.S. Patent Nos. 5,699,157; 5,779,868; and 5,800,690.
- U.S. Patent No. 5,716,852 teaches yet another method for analyzing the presence and concentration of small particles in a flow cell using diffusion principles.
- This patent discloses a channel cell system for detecting the presence of analyte particles in a sample stream using a laminar flow channel having at least two inlet means which provide an indicator stream and a sample stream, where the laminar flow channel has a depth sufficiently small to allow laminar flow of the streams and length sufficient to allow diffusion of particles of the analyte into the indicator stream to form a detection area, and having an outlet out of the channel to form a single mixed stream.
- This device which is known as a T-Sensor, contains an external detecting means for detecting changes in the indicator stream.
- This detecting means may be provided by any means known in the art, including optical means such as optical spectroscopy, or absorption spectroscopy or fluorescence.
- a sample microfluidic analysis instrument for performing analytical testing which uses a disposable fluidic analysis cartridge is disclosed in U.S. Patent Application Serial No. 09/080,691 , which was filed on May 18, 1998, the disclosure of which is incorporated herein by reference.
- This instrument includes a cartridge holder, a flow cytometnc measuring apparatus positioned for optical coupling with a flow cytometric measuring region on the cartridge, and a second measuring apparatus positioned to be coupled with a second analysis region on the cartridge.
- the cartridge holder includes alignment markings to mate with cartridge alignment markings. It also includes pump mechanisms to coupled with pump interfaces on the cartridges and valve mechanisms to couple with valve interfaces on the cartridge.
- valve and pump mechanisms are external to the cartridge, while the cartridge includes the valve and pump interfaces.
- the valve and pump mechanisms engage the valve and pump interfaces.
- the interfaces provide an efficient and precise coupling between the cartridge and the external mechanisms.
- these external devices provide for a smooth flow of the fluids into and out of the cartridge to ensure accurate measurements within a microfluidic analysis system.
- FIG. 1 is an exploded view of a linear pump for use in the present invention
- FIG. 2 is a side elevational view of the pump of FIG. 1 ;
- FIG. 3 is an end view of the pump of FIG. 1 ;
- FIG. 4 is a top elevational view of the differential pump system of the present invention.
- FIG. 5 is an end view of the differential pump of FIG. 4;
- FIG. 6 is a plan view of the fluidic capacitor assembly of the present invention.
- FIG. 7 is a cross-sectional view of the face seal assembly of the present invention.
- FIGS. 1 -3 a linear pump assembly, generally indicated at 10, which embodies the principles of the present invention.
- Pump 10 consists of a linear stepper motor unit 12, a mounting bracket 14, an optical switch 16, a precision bore glass syringe 18, a zero dead volume manifold 20, and a valve 22. Also included is a plurality of screws 24 for attaching manifold 20 to bracket 14, a pair of screws 26 and clinch nuts 28 for mounting switch 16 within bracket 14, and a pair of screws 30 and clinch nuts 32 for mounting motor unit 12 to bracket 14.
- motor 12, syringe 18, and manifold 20 are arranged in a linear configuration to minimize off- axis torque.
- Manifold 20 contains a cross channel 34 which connects to valve 22 and to fittings for connection to external fluid components (not shown). To reduce the hold up volume to near zero dead volume, the piston of syringe 18 intrudes into cross channel 34 at the end of its delivery stroke. Valve 22 is embedded within manifold 20 with short interconnections for the input and output of pump assembly 10.
- linear stepper motor assembly 10 converts rotational movement to linear displacement via motor unit 12 coupled to syringe 18.
- Motor unit 12 offers movement per step of .001 inch and .00025 inch with a total stroke length of .520 inches.
- the bores of syringe 18 can range from .028 to .583 inches in diameter. By combining these components in different arrangements, a total volume displacement can range from 5 microliters to 4 milliiiters.
- Stepper motor unit 12 is controlled using a microstepper controller (not shown) which further portitions each full step to as much as 256 microsteps.
- a microstepper controller not shown
- flow rates can be as low as 1/256 of the full step rate.
- Flow rates using pump assembly 12 range from .0025 microliter/step to 4.314 microliter/step with a consistent and smooth flow rate to insure accuracy.
- FIGS. 4 and 5 illustrate an alternative embodiment of the present invention which employs a differential pump system.
- a differential pump system generally designated at 50, includes a mounting bracket 52, a pair of linear stepper motor units 54a and 54b, a pair of glass syringes 56a and 56b corresponding to motor units 54a and 54b, a pair of optical switches 58a and 58b corresponding to motor units 54a and 54b, and a manifold 60 having a cross channel 62.
- Syringes 56a and 56b are coupled to motor units 54a and 54b to translate rotary motion into linear motion.
- Pumps 54a and 54b are driven differentially, with one advancing while the other is receding.
- the net output from cross channel 62 will be the difference in the velocities of the two pumps.
- the velocities and displacements of the individual pumps are located within a range above the conditions where elasticity and stiction are a major component of the volume displacement, whereby providing a constant and smooth fluid flow within the system to increase the accuracy of the analysis performed by the system.
- FIG. 6 Another device which is helpful in reducing fluctuation in the fluid flow of the microfluidic analysis system is shown in FIG. 6.
- a fluidic filter assembly generally designated at 70.
- Assembly 70 includes an input port 72, an output port 74, a restrictor section 75, an accumulator section 76 which terminates in an end cap 78, and a T-connector 80 which couples input port 72 to accumulator 76.
- Assembly 70 acts as a fluidic filter which reduces any fluid pulses that are introduced into the fluid discharge by stiction and/or stepper motor cogging.
- Input port 72 includes a section of tubing 82 which is preferably constructed from .04 inch internal diameter (ID) tubing in the present embodiment.
- Tubing 82 is coupled to restrictor section 75, which consists of a section of smaller tubing 84, by a connector 86.
- Tubing 84 consists of a section of .005 inch ID tubing that is at least 1 inch long, and is coupled at its opposite end to T-connector 80.
- Accumulator section 76 is constructed from a section of tubing 88 which has an ID of .06 inches in the present embodiment, and is 8 inches long.
- Tubing 88 couples T-connector 80 to accumulator section 76.
- Output port 74 is coupled to T-connector 80 by a section of tubing 90 having an ID of .04 inches.
- fluid enters input port 72 via tubing 82 and passes through T- connector 80 which includes the accumulator section 76 which then connects to tubing 84, which acts as a flow restrictor, as it has a smaller ID.
- This assembly configuration acts as a filter to reduce any pulsing discharge to an acceptable smooth and continuous flow level.
- the fluid flow then exits port 74 via tubing 90 which is the same size as the input line.
- a face seal assembly generally designated at 100, consists of a thin flexible elastomenc gasket 102 which is located within a fluidic analysis cartridge 104 as taught in Serial No. 09/808,191. Gasket 102 is located between a top plate 106 and a bottom plate 108 of cartridge 104, and is held in position by a laminate section 1 10. A ring-shaped surface 1 12 of gasket 102 is positioned between the lower surface 102a of gasket 102 and the upper surface 1 10a of laminate section 110 opposite a port 1 14 within cartridge 104.
- a locating surface 116 of the device mates within an aperture 1 18 of cartridge 104 to ensure proper orientation.
- an extension 120 from a manifold 122 of the device is forced against gasket 102, causing a face seal between the two parts, and thus allowing fluid discharge from extension 120 to enter cartridge 104 in a smooth and efficient manner.
- Gasket 102 is constructed such that is provides a force against the bottom surface of extension 120, guaranteeing an adequate sealing force between the surfaces. Multiple face seals are employed within cartridge 104 to provide for multiple reagent and/or sample inputs.
- extension 120 which in the present embodiment is a tubular structure having an internal passageway 124, interacts with flexible gasket 102 to provide a spring loading effect which acts to hold the bottom surface of extension 120 tightly against gasket 102.
Landscapes
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU44498/00A AU4449800A (en) | 1999-03-19 | 2000-03-16 | Pulse damper |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12517099P | 1999-03-19 | 1999-03-19 | |
US60/125,170 | 1999-03-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2000057173A1 WO2000057173A1 (fr) | 2000-09-28 |
WO2000057173A9 true WO2000057173A9 (fr) | 2001-11-15 |
Family
ID=22418501
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2000/006809 WO2000057173A1 (fr) | 1999-03-19 | 2000-03-16 | Amortisseur de pulsations |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU4449800A (fr) |
WO (1) | WO2000057173A1 (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002029106A2 (fr) * | 2000-10-03 | 2002-04-11 | California Institute Of Technology | Dispositifs microfluidiques et procedes d'utilisation |
US9746412B2 (en) | 2012-05-30 | 2017-08-29 | Iris International, Inc. | Flow cytometer |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1464024A (en) * | 1974-09-13 | 1977-02-09 | Pye Ltd | Pulse dampers for liquid chromatography |
US4629562A (en) * | 1985-08-06 | 1986-12-16 | Scientific Systems, Inc. | Pulse dampener |
-
2000
- 2000-03-16 WO PCT/US2000/006809 patent/WO2000057173A1/fr active Application Filing
- 2000-03-16 AU AU44498/00A patent/AU4449800A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2000057173A1 (fr) | 2000-09-28 |
AU4449800A (en) | 2000-10-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7223371B2 (en) | Microfluidic channel network device | |
Verpoorte et al. | Three-dimensional micro flow manifolds for miniaturized chemical analysis systems | |
US4607526A (en) | Particle analysis system | |
US6852284B1 (en) | Liquid analysis cartridge | |
JP2826841B2 (ja) | マルチモード差動容積形ポンプ及び該ポンプにより計量された容積流体の混合方法 | |
US8961902B2 (en) | Method and apparatus for analyte processing | |
US7516641B2 (en) | Flow sensor methods and apparatus | |
US5630706A (en) | Multichannel pump apparatus with microflow rate capability | |
AU2011235038B2 (en) | Biologic fluid analysis system with sample motion | |
EP1279436A2 (fr) | Manipulation et dispense d'un liquide au travers d'un microcanal dans un substrat élastique par compression progressive du canal | |
Greenwood et al. | Sample manipulation in micro total analytical systems | |
JP6526927B1 (ja) | 流体蠕動層ポンプ | |
US20100158720A1 (en) | Valveless micropump | |
US4859422A (en) | Analysis system | |
JP2011506939A (ja) | 溶液リザーバ−ポンプチャンバを有するマイクロ流体カートリッジ | |
CN113008765A (zh) | 一种采用可变形微通道的癌细胞动态行为检测*** | |
JP3787578B2 (ja) | マイクロチップの微細流路における液体送液方法 | |
WO1999053973A2 (fr) | Seringue destinee a un appareil de manutention des fluides | |
WO2000057173A9 (fr) | Amortisseur de pulsations | |
CN113039424A (zh) | 提供高重复性的微流体样品制备设备 | |
Renzi | Fluid injection microvalve | |
Partel et al. | Development of a Low Pressure Syringe Pump for Detecting Cannabinoids Through Liquid Chromatography |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AU CA JP |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
AK | Designated states |
Kind code of ref document: C2 Designated state(s): AU CA JP |
|
AL | Designated countries for regional patents |
Kind code of ref document: C2 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE |
|
COP | Corrected version of pamphlet |
Free format text: PAGES 1-5, DESCRIPTION, REPLACED BY NEW PAGES 1-9; PAGE 6, CLAIMS, REPLACED BY NEW PAGES 10 AND 11;PAGES 1/3-3/3, DRAWINGS, REPLACED BY NEW PAGES 1/3-3/3; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE |
|
122 | Ep: pct application non-entry in european phase |