WO2000056869A3 - Surfactant-lipase complex immobilized on insoluble matrix - Google Patents

Surfactant-lipase complex immobilized on insoluble matrix Download PDF

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Publication number
WO2000056869A3
WO2000056869A3 PCT/IL2000/000166 IL0000166W WO0056869A3 WO 2000056869 A3 WO2000056869 A3 WO 2000056869A3 IL 0000166 W IL0000166 W IL 0000166W WO 0056869 A3 WO0056869 A3 WO 0056869A3
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WO
WIPO (PCT)
Prior art keywords
surfactant
insoluble matrix
complex immobilized
lipase complex
lipase
Prior art date
Application number
PCT/IL2000/000166
Other languages
French (fr)
Other versions
WO2000056869A2 (en
Inventor
Sobhi Basheer
Original Assignee
Enzymotec Ltd
Sobhi Basheer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enzymotec Ltd, Sobhi Basheer filed Critical Enzymotec Ltd
Priority to AU33206/00A priority Critical patent/AU773466B2/en
Priority to EP00911221A priority patent/EP1163329A2/en
Priority to NZ514271A priority patent/NZ514271A/en
Priority to IL14550300A priority patent/IL145503A0/en
Priority to CA002368179A priority patent/CA2368179A1/en
Priority to JP2000606728A priority patent/JP2002539782A/en
Publication of WO2000056869A2 publication Critical patent/WO2000056869A2/en
Publication of WO2000056869A3 publication Critical patent/WO2000056869A3/en
Priority to IL145503A priority patent/IL145503A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6454—Glycerides by esterification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Inorganic Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A lipase preparation comprising an insoluble matrix and a surfactant-coated lipase complex immobilized onto said insoluble matrix. Method of preparation and the use of the novel products are disclosed.
PCT/IL2000/000166 1997-09-24 2000-03-16 Surfactant-lipase complex immobilized on insoluble matrix WO2000056869A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU33206/00A AU773466B2 (en) 1997-09-24 2000-03-16 Surfactant-lipase complex immobilized on insoluble matrix
EP00911221A EP1163329A2 (en) 1999-03-22 2000-03-16 Surfactant-lipase complex immobilized on insoluble matrix
NZ514271A NZ514271A (en) 1999-03-22 2000-03-16 Surfactant-lipase complex immobilized on insoluble matrix
IL14550300A IL145503A0 (en) 1999-03-22 2000-03-16 Sufactant-lipase complex immobilized on insoluble matrix
CA002368179A CA2368179A1 (en) 1999-03-22 2000-03-16 Surfactant-lipase complex immobilized on insoluble matrix
JP2000606728A JP2002539782A (en) 1999-03-22 2000-03-16 Surfactant-lipase complex immobilized on an insoluble matrix
IL145503A IL145503A (en) 1999-03-22 2001-09-17 Surfactant-lipase complex immobilized on insoluble matrix

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL129086 1999-03-22
IL12908699A IL129086A0 (en) 1999-03-22 1999-03-22 Surfactant-lipase complex immobilized on insoluble matrix

Publications (2)

Publication Number Publication Date
WO2000056869A2 WO2000056869A2 (en) 2000-09-28
WO2000056869A3 true WO2000056869A3 (en) 2001-02-08

Family

ID=11072627

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2000/000166 WO2000056869A2 (en) 1997-09-24 2000-03-16 Surfactant-lipase complex immobilized on insoluble matrix

Country Status (7)

Country Link
EP (1) EP1163329A2 (en)
JP (1) JP2002539782A (en)
KR (1) KR100774281B1 (en)
CA (1) CA2368179A1 (en)
IL (2) IL129086A0 (en)
NZ (1) NZ514271A (en)
WO (1) WO2000056869A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102160651B (en) * 2003-09-25 2016-12-14 酶学技术有限公司 Stable formulations of phosphatidylserine

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL142952A (en) 2001-05-03 2005-12-18 Enzmotec Ltd Process for enzyme-catalyzed production of 1,2 diacylated phospholipids
KR100485090B1 (en) * 2002-01-14 2005-04-22 주식회사 포스코 Enzymes coated with ionic liquid
IL152290A0 (en) * 2002-10-14 2003-05-29 Enzymotec Ltd Immobilization of compounds on polymeric matrix
US20060233863A1 (en) 2003-02-10 2006-10-19 Enzymotec Ltd. Oils enriched with diacylglycerols and phytosterol esters and unit dosage forms thereof for use in therapy
IL158139A0 (en) 2003-09-25 2004-09-27 Enzymotec Ltd Stabilized formulations of phosphatidyl serine
US20050130937A1 (en) 2003-10-22 2005-06-16 Enzymotec Ltd. Lipids containing omega-3 and omega-6 fatty acids
US8052992B2 (en) 2003-10-22 2011-11-08 Enzymotec Ltd. Glycerophospholipids containing omega-3 and omega-6 fatty acids and their use in the treatment and improvement of cognitive functions
IL158555A0 (en) 2003-10-22 2004-05-12 Enzymotec Ltd Human breast milk lipid mimetic as dietary supplement
MY142014A (en) 2004-04-08 2010-08-16 Nisshin Oillio Group Ltd A lipase powder, methods for producing the same and use thereof
WO2006016363A2 (en) 2004-08-10 2006-02-16 Enzymotec Ltd. Mixture of phytosterol ester(s) and 1, 3-diglyceride(s) for use in the treatment of medical conditions
US7473539B2 (en) * 2004-09-20 2009-01-06 Sunho Biodiesel Corporation Methods for producing alkyl esters
JP4785174B2 (en) * 2004-12-10 2011-10-05 株式会社豊田中央研究所 Membrane protein composite material and method for producing the same
DK2439268T3 (en) * 2005-06-16 2016-02-01 Dsm Nutritional Products Ag Immobilized enzymes and methods of use thereof
JP4917349B2 (en) * 2006-05-11 2012-04-18 日清オイリオグループ株式会社 Recovery method of lipase activity
IL180598A0 (en) 2007-01-08 2007-07-04 Basheer Sobhi Immobilized interfacial enzymes of improved and stabilized activity
IL183084A0 (en) 2007-05-09 2007-09-20 Trans Biodisel Ltd Modified-immobilized enzymes of high tolerance to hydrophilic substrates in organic media
DE102008006716A1 (en) * 2008-01-30 2009-08-13 BAM Bundesanstalt für Materialforschung und -prüfung Lipasenformulierung
US8846338B2 (en) 2008-08-07 2014-09-30 Lipogen Ltd. Processes for the preparation of phosphatides
ES2536431T3 (en) 2010-04-26 2015-05-25 Enzymotec Ltd. Procedures and lipid compositions to promote the development of intestinal flora
MY173146A (en) * 2014-07-22 2019-12-31 Nisshin Oillio Group Ltd Powdered fat/oil composition, food including powdered fat/oil composition, and methods for producing same
US11147285B2 (en) 2015-09-24 2021-10-19 The Nisshin Oillio Group, Ltd. Powdery fat or oil composition and method for producing same
EP3406695A4 (en) 2016-01-21 2019-09-11 The Nisshin OilliO Group, Ltd. Powderizing agent for liquid component
JP6208921B1 (en) 2016-01-21 2017-10-04 日清オイリオグループ株式会社 Liquid component thickener
WO2017126666A1 (en) 2016-01-21 2017-07-27 日清オイリオグループ株式会社 Thickener for liquid component
JP6216099B1 (en) 2016-01-21 2017-10-18 日清オイリオグループ株式会社 Liquid component powdering agent

Citations (3)

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Publication number Priority date Publication date Assignee Title
US5342768A (en) * 1988-11-16 1994-08-30 Novo Nordisk A/S Immobilization of thermostable microbial lipase by adsorption to macroporous inorganic carrier particles
EP0882798A1 (en) * 1997-06-06 1998-12-09 Akzo Nobel N.V. Process for the preparation of a monoester-containing esterification product of a polyoxyalkylated sucrose
WO1999015689A1 (en) * 1997-09-24 1999-04-01 Enzymothec Ltd. Surfactant-lipase complex immobilized on insoluble matrix

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JP3344738B2 (en) * 1992-08-20 2002-11-18 天野エンザイム株式会社 Immobilized modifying enzyme, ester synthesis method using the immobilized modifying enzyme
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US5342768A (en) * 1988-11-16 1994-08-30 Novo Nordisk A/S Immobilization of thermostable microbial lipase by adsorption to macroporous inorganic carrier particles
EP0882798A1 (en) * 1997-06-06 1998-12-09 Akzo Nobel N.V. Process for the preparation of a monoester-containing esterification product of a polyoxyalkylated sucrose
WO1999015689A1 (en) * 1997-09-24 1999-04-01 Enzymothec Ltd. Surfactant-lipase complex immobilized on insoluble matrix

Non-Patent Citations (4)

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Title
BASHEER SOBHI ET AL: "Esterification kinetics of long-chain acids and fatty alcohols with a surfactant-coated lipase in n-hexane.", JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY, vol. 75, no. 12, December 1998 (1998-12-01), pages 1785 - 1790, XP002149809, ISSN: 0003-021X *
BASHEER SOBHI ET AL: "Surfactant-modified lipase for the catalysis of the interesterification of triglycerides and fatty acids.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 45, no. 3, 1995, pages 187 - 195, XP002915511, ISSN: 0006-3592 *
FISHMAN AYELET ET AL: "Fatty-acid-modified enzymes as enantioselective catalysts in microaqueous organic media.", BIOTECHNOLOGY LETTERS, vol. 20, no. 6, June 1998 (1998-06-01), pages 535 - 538, XP000952631, ISSN: 0141-5492 *
GOTO M ET AL: "ENZYMATIC INTERESTERIFICATION OF TRIGLYCERIDE WITH SURFACTANT-COATED LIPASE IN ORGANIC MEDIA", BIOTECHNOLOGY AND BIOENGINEERING. INCLUDING: SYMPOSIUM BIOTECHNOLOGY IN ENERGY PRODUCTION AND CONSERVATION,US,JOHN WILEY & SONS. NEW YORK, vol. 45, no. 1, 5 January 1995 (1995-01-05), pages 27 - 32, XP000489584, ISSN: 0006-3592 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102160651B (en) * 2003-09-25 2016-12-14 酶学技术有限公司 Stable formulations of phosphatidylserine

Also Published As

Publication number Publication date
CA2368179A1 (en) 2000-09-28
NZ514271A (en) 2003-08-29
KR20020010129A (en) 2002-02-02
KR100774281B1 (en) 2007-11-08
IL145503A (en) 2009-06-15
WO2000056869A2 (en) 2000-09-28
EP1163329A2 (en) 2001-12-19
JP2002539782A (en) 2002-11-26
IL129086A0 (en) 2000-02-17

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