WO2000044906A3 - GENES IDENTIFIED AS REQUIRED FOR PROLIFERATION IN $i(ESCHERICHIA COLI) - Google Patents

GENES IDENTIFIED AS REQUIRED FOR PROLIFERATION IN $i(ESCHERICHIA COLI) Download PDF

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Publication number
WO2000044906A3
WO2000044906A3 PCT/US2000/002200 US0002200W WO0044906A3 WO 2000044906 A3 WO2000044906 A3 WO 2000044906A3 US 0002200 W US0002200 W US 0002200W WO 0044906 A3 WO0044906 A3 WO 0044906A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
proliferation
screen
proteins
coli
Prior art date
Application number
PCT/US2000/002200
Other languages
French (fr)
Other versions
WO2000044906A2 (en
Inventor
Judith Zyskind
Kari L Ohlsen
John D Trawick
R Allyn Forsyth
Jamie M Froelich
Grant J Carr
Robert T Yamamoto
H Howard Xu
Original Assignee
Elitra Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elitra Pharmaceuticals Inc filed Critical Elitra Pharmaceuticals Inc
Priority to EP00914458A priority Critical patent/EP1149166A2/en
Priority to JP2000596147A priority patent/JP2002535007A/en
Priority to CA002360085A priority patent/CA2360085A1/en
Priority to KR1020017009547A priority patent/KR20010101861A/en
Priority to NZ512835A priority patent/NZ512835A/en
Priority to AU35845/00A priority patent/AU3584500A/en
Publication of WO2000044906A2 publication Critical patent/WO2000044906A2/en
Publication of WO2000044906A3 publication Critical patent/WO2000044906A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Wood Science & Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The sequences of nucleic acids encoding proteins required for E. coli proliferation are disclosed. The nucleic acids can be used to express proteins or portions thereof, to obtain antibodies capable of specifically binding to the expressed proteins, and to use those expressed proteins as a screen to isolate candidate molecules for rational drug discovery programs. The nucleic acids can also be used to screen for homologous genes that are required for proliferation in microorganisms other than E. coli. The nucleic acids can also be used to design expression vectors and secretion vectors. The nucleic acids of the present invention can also be used in various assay systems to screen for proliferation required genes in other organisms as well as to screen for antimicrobial agents.
PCT/US2000/002200 1999-01-27 2000-01-27 GENES IDENTIFIED AS REQUIRED FOR PROLIFERATION IN $i(ESCHERICHIA COLI) WO2000044906A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP00914458A EP1149166A2 (en) 1999-01-27 2000-01-27 GENES IDENTIFIED AS REQUIRED FOR PROLIFERATION IN $i(ESCHERICHIA COLI)
JP2000596147A JP2002535007A (en) 1999-01-27 2000-01-27 Genes identified as required for the growth of Escherichia coli
CA002360085A CA2360085A1 (en) 1999-01-27 2000-01-27 Genes identified as required for proliferation in escherichia coli
KR1020017009547A KR20010101861A (en) 1999-01-27 2000-01-27 Genes Identified as Required for Proliferation in Escherichia coli
NZ512835A NZ512835A (en) 1999-01-27 2000-01-27 Genes identified as required for proliferation in escherichia coli
AU35845/00A AU3584500A (en) 1999-01-27 2000-01-27 Genes identified as required for proliferation in escherichia coli

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11740599P 1999-01-27 1999-01-27
US60/117,405 1999-01-27

Publications (2)

Publication Number Publication Date
WO2000044906A2 WO2000044906A2 (en) 2000-08-03
WO2000044906A3 true WO2000044906A3 (en) 2001-02-01

Family

ID=22372748

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/002200 WO2000044906A2 (en) 1999-01-27 2000-01-27 GENES IDENTIFIED AS REQUIRED FOR PROLIFERATION IN $i(ESCHERICHIA COLI)

Country Status (7)

Country Link
EP (1) EP1149166A2 (en)
JP (1) JP2002535007A (en)
KR (1) KR20010101861A (en)
AU (1) AU3584500A (en)
CA (1) CA2360085A1 (en)
NZ (1) NZ512835A (en)
WO (1) WO2000044906A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1130094A3 (en) * 1999-07-08 2001-11-21 Helix Research Institute Primers for synthesizing full length cDNA clones and their use
WO2001027262A1 (en) * 1999-10-13 2001-04-19 Pantheco A/S Gene selection using pnas
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
WO2002079467A2 (en) * 2001-03-29 2002-10-10 Københavns Universitet Antibiotic-free bacterial strain selection with antisense molecules
AU2002355155A1 (en) * 2001-07-24 2003-02-17 Affinium Pharmaceuticals Inc. Methods for gene disruption and uses thereof
FR2842210B1 (en) * 2002-07-09 2012-12-14 Mutabilis PATHOGENICITY DETERMINANTS FOR USE AS TARGETS FOR PREPARING AND CONTROLLING BACTERIAL INFECTIONS AND / OR SYSTEMIC DISSEMINATION
JPWO2007013411A1 (en) * 2005-07-25 2009-02-05 株式会社カネカ Biological antisense nucleic acid
EP1940443A4 (en) * 2005-09-01 2009-11-11 The Feinstein Inst Medical Res Peptide mimics of melanocyte stimulating hormone
DK2482836T3 (en) 2009-10-02 2016-06-06 Univ Oslo Hf Antibacterial polypeptides and the use thereof
WO2012048249A1 (en) 2010-10-07 2012-04-12 Yale University Site-specific incorporation of phosphoserine into proteins in escherichia coli
US11788111B2 (en) 2011-07-11 2023-10-17 Yale University Compositions and methods for making selenocysteine containing polypeptides
US10240158B2 (en) 2011-07-11 2019-03-26 Yale University Compositions and methods for making selenocysteine containing polypeptides
US10876142B2 (en) 2011-07-11 2020-12-29 Yale University Compositions and methods for making selenocysteine containing polypeptides
US9464288B2 (en) 2011-07-11 2016-10-11 Yale University Compositions and methods for making selenocysteine containing polypeptides
BR112021014805A2 (en) * 2019-01-31 2022-01-04 Spiber Inc Production methods for a recombinant protein and for increasing production volume
CN113917140B (en) * 2021-10-28 2023-08-18 生工生物工程（上海）股份有限公司 Method for rapidly screening prokaryotic protein expression bacteria
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998021366A1 (en) * 1996-11-13 1998-05-22 Qbi Enterprises, Ltd. Gene identification method
WO1999002673A2 (en) * 1997-07-10 1999-01-21 Genesense Technologies, Inc. Antisense oligonucleotide sequences as inhibitors of microorganisms

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998021366A1 (en) * 1996-11-13 1998-05-22 Qbi Enterprises, Ltd. Gene identification method
WO1999002673A2 (en) * 1997-07-10 1999-01-21 Genesense Technologies, Inc. Antisense oligonucleotide sequences as inhibitors of microorganisms

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AUSTIN A.E. ET AL.: "Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli k12 insertion mutagenesis of the RFA locus", JOURNAL OF BACTERIOLOGY, vol. 172, 1990, WASHINGTON US, pages 5312 - 5325, XP000926028 *
BLATTNER F R ET AL: "THE COMPLETE GENOME SEQUENCE OF ESCHERICHIA COLI K-12", SCIENCE., vol. 277, 5 September 1997 (1997-09-05), LANCASTER, PA., US, pages 1453 - 1462, XP002923023, ISSN: 0036-8075 *
LEE N.G. ET AL.: "Molecular cloning and characterization of the nontypable Haemophilus influenzae-2019 rfaE gene required for lipopolysaccharide biosynthesis", INFECTION AND IMMUNITY., vol. 63, no. 3, 1995, WASHINGTON., US, pages 818 - 824, XP000953326, ISSN: 0019-9567 *
POST L E ET AL: "NUCLEOTIDE SEQUENCE OF THE RIBOSOMAL PROTEIN GENE CLUSTER ADJACENT TO THE GENE FOR RNA POLYMERASE SUBUNIT BETA IN ESCHERICHIA COLI", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA,US,NEW YORK, NY, vol. 76, no. 4, 1 April 1979 (1979-04-01), pages 1697 - 1701, XP000574791 *
VALVANO M.A. ET AL.: "The rfaE gene from Escherichia coli encodes a bifunctional protein involved in biosynthesis of the lipopolysaccharide core precursor ADP-L-glycero-D-manno-heptose.", JOURNAL OF BACTERIOLOGY, vol. 182, January 2000 (2000-01-01), WASHINGTON US, pages 488 - 497, XP000926030 *
VAN HEESWIJK W.C. ET AL.: "The genes of the glutamine synthetase adenylylation cascade are not regulated by nitrogen in Escherichia coli", MOLECULAR MICROBIOLOGY, vol. 9, 1993, OXFORD GB, pages 443 - 457, XP000926027 *

Also Published As

Publication number Publication date
CA2360085A1 (en) 2000-08-03
AU3584500A (en) 2000-08-18
KR20010101861A (en) 2001-11-15
EP1149166A2 (en) 2001-10-31
NZ512835A (en) 2004-02-27
WO2000044906A2 (en) 2000-08-03
JP2002535007A (en) 2002-10-22

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