WO2000015772A3 - Recombinant protein production in urine - Google Patents

Recombinant protein production in urine Download PDF

Info

Publication number
WO2000015772A3
WO2000015772A3 PCT/IB1999/001609 IB9901609W WO0015772A3 WO 2000015772 A3 WO2000015772 A3 WO 2000015772A3 IB 9901609 W IB9901609 W IB 9901609W WO 0015772 A3 WO0015772 A3 WO 0015772A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
urine
secreted
gene promoter
animal
Prior art date
Application number
PCT/IB1999/001609
Other languages
French (fr)
Other versions
WO2000015772A9 (en
WO2000015772A2 (en
Inventor
Costas N Karatzas
Original Assignee
Nexia Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nexia Biotech Inc filed Critical Nexia Biotech Inc
Priority to EP99944741A priority Critical patent/EP1112353A2/en
Priority to JP2000570299A priority patent/JP2002525047A/en
Priority to AU57553/99A priority patent/AU5755399A/en
Priority to CA002343104A priority patent/CA2343104A1/en
Publication of WO2000015772A2 publication Critical patent/WO2000015772A2/en
Publication of WO2000015772A3 publication Critical patent/WO2000015772A3/en
Publication of WO2000015772A9 publication Critical patent/WO2000015772A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2839—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily
    • C07K16/2842—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily against integrin beta1-subunit-containing molecules, e.g. CD29, CD49
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/40—Vector systems having a special element relevant for transcription being an insulator
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides methods which generate a polypeptide that is secreted into the urine by using the uromodulin gene promoter, or other promoters from genes whose products are specifically expressed in the kidney, to direct the expression of the secreted polypeptide. Also disclosed is a transgenic animal secreting a recombinant polypeptide into its urine, as well as a method for detecting such an animal while the animal is in utero. Also disclosed are methods for generating a polypeptide that is secreted into the conditioned media of cultured kidney cells transformed with a polypeptide-encoding nucleic acid sequence that is operably linked to a uromodulin gene promoter, or other kidney specific gene promoter.
PCT/IB1999/001609 1998-09-16 1999-09-16 Recombinant protein production in urine WO2000015772A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP99944741A EP1112353A2 (en) 1998-09-16 1999-09-16 Recombinant protein production in urine
JP2000570299A JP2002525047A (en) 1998-09-16 1999-09-16 Production of recombinant protein in urine
AU57553/99A AU5755399A (en) 1998-09-16 1999-09-16 Recombinant protein production in urine
CA002343104A CA2343104A1 (en) 1998-09-16 1999-09-16 Recombinant protein production in urine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10054098P 1998-09-16 1998-09-16
US60/100,540 1998-09-16

Publications (3)

Publication Number Publication Date
WO2000015772A2 WO2000015772A2 (en) 2000-03-23
WO2000015772A3 true WO2000015772A3 (en) 2000-07-20
WO2000015772A9 WO2000015772A9 (en) 2000-08-24

Family

ID=22280279

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1999/001609 WO2000015772A2 (en) 1998-09-16 1999-09-16 Recombinant protein production in urine

Country Status (5)

Country Link
EP (1) EP1112353A2 (en)
JP (1) JP2002525047A (en)
AU (1) AU5755399A (en)
CA (1) CA2343104A1 (en)
WO (1) WO2000015772A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1621900A (en) * 1998-11-13 2000-06-05 New York University Transgenic animals as urinary bioreactors for the production of protein in the urine, recombinant dna construct for kidney-specific expression, and method of using same
US6888047B1 (en) 1998-11-13 2005-05-03 New York University Transgenic animals as urinary bioreactors for the production of polypeptide in the urine, recombinant DNA construct for kidney-specific expression, and method of using same
AU2492400A (en) * 1999-01-06 2000-07-24 Atlantic Biopharmaceuticals, Inc. Expression of secreted human alpha-fetoprotein in transgenic animals
KR100458792B1 (en) * 2002-04-10 2004-12-03 주식회사 인투젠 Expression of a heterologous polypeptide in renal tissue of transgenic non-human mammals using promoter for tamm-horsfall uromodulin protein
US20080014215A1 (en) * 2003-05-27 2008-01-17 Blattner Frederick R Animal Bioreactors
WO2011084145A2 (en) 2009-12-21 2011-07-14 Pharmathene, Inc. Recombinant butyrylcholinesterases and truncates thereof
US9963511B2 (en) * 2011-12-22 2018-05-08 Hoffmann-La Roche Inc. Expression vector organization, novel production cell generation methods and their use for the recombinant production of polypeptides
AU2016315699B2 (en) * 2015-08-31 2021-09-09 The Trustees Of The University Of Pennsylvania AAV-EPO for treating companion animals
EP3589306B1 (en) * 2017-02-28 2022-04-27 Charité Universitätsmedizin Berlin Uromodulin for use in prevention and therapy of vascular calcification or vascular inflammation
WO2019045049A1 (en) * 2017-09-01 2019-03-07 学校法人帝京大学 Renal tubule cell-specific expression vector

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022249A1 (en) * 1994-02-18 1995-08-24 American National Red Cross Transgenic fibrinogen
FR2717500A1 (en) * 1994-03-17 1995-09-22 Pasteur Institut Use of recombinant vector encoding specific protein to transform kidney cells
WO1996026268A1 (en) * 1995-02-21 1996-08-29 Genzyme Transgenics Corporation Transgenically produced antithrombin iii
WO1996039494A1 (en) * 1995-06-05 1996-12-12 New York University Method for expression and isolation of biologically active molecules in urine
WO1997019589A1 (en) * 1995-11-30 1997-06-05 Nexia Biotechnologies, Inc. Method for development of transgenic goats
WO1999047661A2 (en) * 1998-03-17 1999-09-23 Nexia Biotechnologies, Inc. Production of biofilaments in transgenic animals

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022249A1 (en) * 1994-02-18 1995-08-24 American National Red Cross Transgenic fibrinogen
FR2717500A1 (en) * 1994-03-17 1995-09-22 Pasteur Institut Use of recombinant vector encoding specific protein to transform kidney cells
WO1996026268A1 (en) * 1995-02-21 1996-08-29 Genzyme Transgenics Corporation Transgenically produced antithrombin iii
WO1996039494A1 (en) * 1995-06-05 1996-12-12 New York University Method for expression and isolation of biologically active molecules in urine
US5824543A (en) * 1995-06-05 1998-10-20 New York University Method for expression and isolation of biologically active molecules in urine using a mouse uroplakin-II promoter
WO1997019589A1 (en) * 1995-11-30 1997-06-05 Nexia Biotechnologies, Inc. Method for development of transgenic goats
WO1999047661A2 (en) * 1998-03-17 1999-09-23 Nexia Biotechnologies, Inc. Production of biofilaments in transgenic animals

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GORDON K ET AL: "EXPRESSIOON OF HUMAN TPA IN THE MILK OF TRANSGENIC ANIMALS", GENE TRANSFER AND GENE THERAPY,US,NEW YORK, NY, 6 February 1988 (1988-02-06), pages 57 - 66, XP000350867 *
KERR D ET AL.,: "The bladder as a bioreactor: Urothelium production and secretion of growth hormone into urine.", NATURE BIOTECHNOLOGY, vol. 16, January 1998 (1998-01-01), pages 75-79, XP002120418 *
POUL M A ET AL.,: "Inhibition of T cell activation with a humanized anti-beta-1 integrin chain mAb", MOLECULAR IMMUNOLOGY, vol. 32, no. 2, 1995, pages 101-116, XP000901077 *
QUIGG R J ET AL.,: "Transgenic mice overexpressing the complement inhibitor Crry as a soluble protein are protected from antibody-induced glomerular injury.", JOURNAL OF EXPERIMENTAL MEDICINE, vol. 88, 5 October 1998 (1998-10-05), pages 1321-1331, XP000893030 *

Also Published As

Publication number Publication date
CA2343104A1 (en) 2000-03-23
WO2000015772A9 (en) 2000-08-24
AU5755399A (en) 2000-04-03
WO2000015772A2 (en) 2000-03-23
EP1112353A2 (en) 2001-07-04
JP2002525047A (en) 2002-08-13

Similar Documents

Publication Publication Date Title
ATE210180T1 (en) FEED SUPPLEMENTS FOR ANIMALS CONTAINING XYLANASE
NZ603037A (en) Fusion constructs and use of same to produce antibodies with increased fc receptor binding affinity and effector function
EP1650306A3 (en) Means and methods for modifying gene expression using unpolyadenylated RNA
BR0316525A (en) Neomycin phosphotransferase genes and processes for selecting high yield recombinant cells
HUT51336A (en) Process for construction of aspergillus strains by gene substitution
CN101679992A (en) Expression system
RU2007124369A (en) PROTEIN PRODUCTION
KR840005745A (en) Controlled plasmid containing hepatitis B virus gene, a method for producing yeast and hepatitis B virus surface antigen converted to this plasmid
WO2000015772A3 (en) Recombinant protein production in urine
ATE542913T1 (en) PROMOTOR VARIANTS FOR EXPRESSING GENES IN A FUNGAL CELL
EP0139501A3 (en) The manufacture and expression of genes for thaumatin
BR9709658A (en) Isolation of pseudo-oligosaccharide biosynthesis genes from streptomyces glaucescens gla o and its application
CN102659928A (en) Synthetic signal peptide and application thereof
CN104066841A (en) Expression vector and method for producing protein
CN101415824B (en) Auto-inducible sodium phosphate symporter promoter from pichia pastoris and method for producing recombinant protein using it
AU5728399A (en) Method for transformation of animal cells
WO2005001099A8 (en) Protein expression system
CN101921315B (en) Artificially synthesized signal peptide and application thereof
KR101724614B1 (en) New Catalase Signal Sequences and Expression Method Using The Same
KR970010968A (en) Expression System of Erythropoin using Duck Germ Cells
CN104059128B (en) The signal peptide of a kind of synthetic and application thereof
AU2001263920A1 (en) Method for the production of proteins in a yeast of the genusarxula and suitable promoters
WO2002024898A3 (en) 47647, a human lipase and uses therefor
AU5876300A (en) Pichia methanolica glyceraldehyde 3-phosphate dehydrogenase 1 promoter and terminator
KR970001543A (en) New promoter and gene expression method using the same

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU BR CA HU JP NZ

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AU BR CA HU JP NZ

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

AK Designated states

Kind code of ref document: C2

Designated state(s): AU BR CA HU JP NZ

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

COP Corrected version of pamphlet

Free format text: PAGES 1-3, 5-8, 11, 15-18 AND 20-31, DESCRIPTION, REPLACED BY NEW PAGES 1-3, 5-8, 11, 15-18 AND 20-31; AFTER RECTIFICATION OF OBVIOUS ERRORS AS AUTHORIZED BY THE INTERNATIONAL SEARCHING AUTHORITY; PAGES 9, 10, 12-14, 19 AND 32, DESCRIPTION, REPLACED BY NEW PAGES 9, 10, 12-14, 19 AND 32; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

WWE Wipo information: entry into national phase

Ref document number: 509900

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 57553/99

Country of ref document: AU

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 570299

Kind code of ref document: A

Format of ref document f/p: F

ENP Entry into the national phase

Ref document number: 2343104

Country of ref document: CA

Ref country code: CA

Ref document number: 2343104

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 1999944741

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999944741

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999944741

Country of ref document: EP