WO1999063074A3 - Altering the ligand-binding characteristics of a nucleic acid ligand binding sequence by altering the nucleotide composition of its flanking sequences - Google Patents

Altering the ligand-binding characteristics of a nucleic acid ligand binding sequence by altering the nucleotide composition of its flanking sequences Download PDF

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Publication number
WO1999063074A3
WO1999063074A3 PCT/US1999/012515 US9912515W WO9963074A3 WO 1999063074 A3 WO1999063074 A3 WO 1999063074A3 US 9912515 W US9912515 W US 9912515W WO 9963074 A3 WO9963074 A3 WO 9963074A3
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WO
WIPO (PCT)
Prior art keywords
ligand
altering
nucleic acid
nucleotide composition
binding
Prior art date
Application number
PCT/US1999/012515
Other languages
French (fr)
Other versions
WO1999063074A2 (en
Inventor
Michael J Lane
Albert S Benight
Brian D Faldasz
Original Assignee
Tm Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Tm Technologies Inc filed Critical Tm Technologies Inc
Publication of WO1999063074A2 publication Critical patent/WO1999063074A2/en
Publication of WO1999063074A3 publication Critical patent/WO1999063074A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

This invention relates to a method of improving the relative expression level of any eukaryotic, prokaryotic, viral or any gene of interest in eukaryotic or prokaryotic cell lines and related methods and products. The invention described herein alters the ligand-binding characteristics of a nucleic acid sequence of any given length without the use of small molecule pharmaceuticals and teaches that the binding affinity of a ligand, such as RNA polymerase and/or transcription factors, for its ligand binding site may be modulated solely by the nucleotide composition of the polynucleotide sequence(s) flanking an adjacent ligand binding site.
PCT/US1999/012515 1998-06-04 1999-06-04 Altering the ligand-binding characteristics of a nucleic acid ligand binding sequence by altering the nucleotide composition of its flanking sequences WO1999063074A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US8794598P 1998-06-04 1998-06-04
US60/087,945 1998-06-04
US32324499A 1999-06-03 1999-06-03
US09/323,244 1999-06-03

Publications (2)

Publication Number Publication Date
WO1999063074A2 WO1999063074A2 (en) 1999-12-09
WO1999063074A3 true WO1999063074A3 (en) 2000-04-06

Family

ID=26777546

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/012515 WO1999063074A2 (en) 1998-06-04 1999-06-04 Altering the ligand-binding characteristics of a nucleic acid ligand binding sequence by altering the nucleotide composition of its flanking sequences

Country Status (1)

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WO (1) WO1999063074A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109750039B (en) * 2019-01-21 2022-09-20 北京大学 Plant touch response promoter and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995000665A1 (en) * 1993-06-17 1995-01-05 The Research Foundation Of State University Of New York Thermodynamics, design, and use of nucleic acid sequences
WO1998037242A1 (en) * 1997-02-24 1998-08-27 Tm Technologies, Inc. Process for selecting anti-sense oligonucleotides
WO1999032664A1 (en) * 1997-12-23 1999-07-01 Tm Technologies, Inc. Method of selecting flanking sequences which convey relative binding affinities to a ligand binding site
WO1999042621A2 (en) * 1998-02-21 1999-08-26 Tm Technologies, Inc. Methods for identifying or characterising a site based on the thermodynamic properties of nucleic acids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995000665A1 (en) * 1993-06-17 1995-01-05 The Research Foundation Of State University Of New York Thermodynamics, design, and use of nucleic acid sequences
WO1998037242A1 (en) * 1997-02-24 1998-08-27 Tm Technologies, Inc. Process for selecting anti-sense oligonucleotides
WO1999032664A1 (en) * 1997-12-23 1999-07-01 Tm Technologies, Inc. Method of selecting flanking sequences which convey relative binding affinities to a ligand binding site
WO1999042621A2 (en) * 1998-02-21 1999-08-26 Tm Technologies, Inc. Methods for identifying or characterising a site based on the thermodynamic properties of nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"Non-contacted sequences flanking a ligand binding site can modulate the affinity of ligand binding: implications of such modulation in single strand to duplex reaction design", BIOPHYSICAL JOURNAL, VOL. 74,NO.2,PART 2, 22 - 26 FEBRUARY 1998, PAGE A5, XP002116814 *

Also Published As

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WO1999063074A2 (en) 1999-12-09

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