WO1999045142A3 - Mittel und verfahren zum nachweis chemischer substanzen - Google Patents

Mittel und verfahren zum nachweis chemischer substanzen Download PDF

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Publication number
WO1999045142A3
WO1999045142A3 PCT/DE1999/000558 DE9900558W WO9945142A3 WO 1999045142 A3 WO1999045142 A3 WO 1999045142A3 DE 9900558 W DE9900558 W DE 9900558W WO 9945142 A3 WO9945142 A3 WO 9945142A3
Authority
WO
WIPO (PCT)
Prior art keywords
group
polynucleotide
peptide sequence
agent
attached
Prior art date
Application number
PCT/DE1999/000558
Other languages
English (en)
French (fr)
Other versions
WO1999045142A2 (de
Inventor
Wolf Bertling
Original Assignee
November Ag Molekulare Medizin
Wolf Bertling
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by November Ag Molekulare Medizin, Wolf Bertling filed Critical November Ag Molekulare Medizin
Priority to JP2000534673A priority Critical patent/JP2002505113A/ja
Priority to EP99917762A priority patent/EP1070144A2/de
Priority to US09/623,581 priority patent/US6468752B1/en
Priority to CA002323171A priority patent/CA2323171A1/en
Publication of WO1999045142A2 publication Critical patent/WO1999045142A2/de
Publication of WO1999045142A3 publication Critical patent/WO1999045142A3/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

Die Erfindung betrifft ein Mittel zum Nachweis chemischer Substanzen oder physikalischer Eigenschaften, mit einer eine erste (3) fluorophore Gruppe aufweisenden ersten Polynukleotid- (1) oder Peptidsequenz, deren erstes Ende (E1) an eine feste Phase (5) gebunden ist und einer eine zweite (4) fluorophore Gruppe aufweisenden zweiten Polynukleotid- (1a) oder Peptidsequenz, deren zweites Ende (E2) eine an die nachzuweisende chemische Substanz (7) bind- bzw. anlagerbare oder eine für die nachzuweisende physikalische Eigenschaft empfindliche Gruppe (6) aufweist, wobei die erste Polynukleotid- (1) oder Peptidsequenz an die zweite Polynukleotid- (1a) oder Peptidsequenz so anlagerbar ist, dass eine eine Wechselwirkung zwischen der ersten (3) und der zweiten fluorophoren Gruppe (4) ermöglichende räumliche Beziehung herstellbar ist, und wobei bei Anlagerung der chemischen Substanz (7) an die Gruppe (6) und/oder Einwirkung einer äußeren Kraft auf die Gruppe (6, 12) die räumliche Beziehung aufheb- und damit eine Fluoreszenzreaktion erzeugbar ist.
PCT/DE1999/000558 1998-03-03 1999-03-02 Mittel und verfahren zum nachweis chemischer substanzen WO1999045142A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000534673A JP2002505113A (ja) 1998-03-03 1999-03-02 化学物質を検出するための試薬と方法
EP99917762A EP1070144A2 (de) 1998-03-03 1999-03-02 Mittel und verfahren zum nachweis chemischer substanzen
US09/623,581 US6468752B1 (en) 1998-03-03 1999-03-02 Agent and method for detecting chemical substances
CA002323171A CA2323171A1 (en) 1998-03-03 1999-03-02 Agent and method for detecting chemical substances

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19808884.1 1998-03-03
DE19808884A DE19808884A1 (de) 1998-03-03 1998-03-03 Vorrichtung und Verfahren zum Nachweis chemischer Substanzen

Publications (2)

Publication Number Publication Date
WO1999045142A2 WO1999045142A2 (de) 1999-09-10
WO1999045142A3 true WO1999045142A3 (de) 1999-10-28

Family

ID=7859475

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/000558 WO1999045142A2 (de) 1998-03-03 1999-03-02 Mittel und verfahren zum nachweis chemischer substanzen

Country Status (6)

Country Link
US (1) US6468752B1 (de)
EP (1) EP1070144A2 (de)
JP (1) JP2002505113A (de)
CA (1) CA2323171A1 (de)
DE (1) DE19808884A1 (de)
WO (1) WO1999045142A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1185709B1 (de) * 1999-06-15 2004-05-12 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Vorrichtung und verfahren zum speichern von informationen
GB2359625B (en) 1999-12-10 2004-10-20 Molecular Light Tech Res Ltd Monitoring oligonucleotide binding process using chemiluminescence quenching
DE19960076C2 (de) * 1999-12-13 2002-12-05 November Ag Molekulare Medizin Verfahren und Vorrichtung zum Nachweis und zur Quantifizierung von Biomolekülen
US20040086883A1 (en) * 2000-08-11 2004-05-06 Hermann Gaub Method and device for characterising and/or for detecting a bonding complex
AU2002321052A1 (en) * 2001-06-01 2002-12-16 Nanotype Gmbh Method for determining an analyte
DE10126798A1 (de) 2001-06-01 2002-12-19 Nanotype Gmbh Verfahren zur Bestimmung einer Probe
DE50210214D1 (de) * 2001-12-24 2007-07-05 Arrowhead Res Corp Verfahren zur in-vitro selektion von bindern
CN100442051C (zh) * 2005-11-28 2008-12-10 李寿椿 4-(n，n－二甲氨基)偶氮苯－4’-磺酰氟及其合成方法和用途
EP2060637A1 (de) * 2007-11-14 2009-05-20 Koninklijke Philips Electronics N.V. Mittel und Verfahren zum Erkennen von Nukleinsäuren
AU2020213460B2 (en) * 2019-01-30 2024-02-29 Suzhou Astrabio Technology Co., Ltd. Single molecule quantitative detection method and detection system

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5156972A (en) * 1989-09-05 1992-10-20 The State Of Israel, Atomic Energy Commission, Soreq Nuclear Research Center Analyte specific chemical sensor with a ligand and an analogue bound on the sensing surface
EP0745690A2 (de) * 1995-05-12 1996-12-04 The Public Health Research Institute Of The City Of New York, Inc. Markierte zwei-konformations oligonukleotid Sonden, Verfahren und Testsätze
EP0762122A1 (de) * 1995-08-16 1997-03-12 Bayer Ag Optischer Festphasenbiosensor auf Basis von Streptavidin und Biotin
WO1997009342A1 (en) * 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna
WO1998051819A1 (en) * 1997-05-14 1998-11-19 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759820A (en) * 1988-11-21 1998-06-02 Dynal As Process for producing cDNA
EP0601889A2 (de) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
US5665558A (en) * 1994-05-17 1997-09-09 Gamma Biologicals, Inc. Method and apparatus useful for detecting bloodgroup antigens and antibodies
US5770365A (en) * 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5156972A (en) * 1989-09-05 1992-10-20 The State Of Israel, Atomic Energy Commission, Soreq Nuclear Research Center Analyte specific chemical sensor with a ligand and an analogue bound on the sensing surface
EP0745690A2 (de) * 1995-05-12 1996-12-04 The Public Health Research Institute Of The City Of New York, Inc. Markierte zwei-konformations oligonukleotid Sonden, Verfahren und Testsätze
EP0762122A1 (de) * 1995-08-16 1997-03-12 Bayer Ag Optischer Festphasenbiosensor auf Basis von Streptavidin und Biotin
WO1997009342A1 (en) * 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna
WO1998051819A1 (en) * 1997-05-14 1998-11-19 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis

Also Published As

Publication number Publication date
EP1070144A2 (de) 2001-01-24
US6468752B1 (en) 2002-10-22
CA2323171A1 (en) 1999-09-10
WO1999045142A2 (de) 1999-09-10
DE19808884A1 (de) 1999-09-16
JP2002505113A (ja) 2002-02-19

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