WO1999035274A3 - Nucleic acids encoding thermostable pullulanases - Google Patents

Nucleic acids encoding thermostable pullulanases Download PDF

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Publication number
WO1999035274A3
WO1999035274A3 PCT/IB1999/000069 IB9900069W WO9935274A3 WO 1999035274 A3 WO1999035274 A3 WO 1999035274A3 IB 9900069 W IB9900069 W IB 9900069W WO 9935274 A3 WO9935274 A3 WO 9935274A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
acids encoding
encoding thermostable
pullulanases
thermostable pullulanases
Prior art date
Application number
PCT/IB1999/000069
Other languages
French (fr)
Other versions
WO1999035274A2 (en
Inventor
Per Linaa Joergensen
Fiona Duffner
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Priority to AU17791/99A priority Critical patent/AU1779199A/en
Publication of WO1999035274A2 publication Critical patent/WO1999035274A2/en
Publication of WO1999035274A3 publication Critical patent/WO1999035274A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the thermostable pullulanases from Fervidobacterium sp. Ven 5.
PCT/IB1999/000069 1998-01-07 1999-01-05 Nucleic acids encoding thermostable pullulanases WO1999035274A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU17791/99A AU1779199A (en) 1998-01-07 1999-01-05 Nucleic acids encoding thermostable pullulanases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US383498A 1998-01-07 1998-01-07
US09/003,834 1998-01-07

Publications (2)

Publication Number Publication Date
WO1999035274A2 WO1999035274A2 (en) 1999-07-15
WO1999035274A3 true WO1999035274A3 (en) 1999-09-16

Family

ID=21707808

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1999/000069 WO1999035274A2 (en) 1998-01-07 1999-01-05 Nucleic acids encoding thermostable pullulanases

Country Status (2)

Country Link
AU (1) AU1779199A (en)
WO (1) WO1999035274A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002519054A (en) * 1998-07-02 2002-07-02 ノボザイムス アクティーゼルスカブ Starch debranching enzyme
US6265197B1 (en) 1998-07-02 2001-07-24 Novozymes A/S Krogshoejvej Starch debranching enzymes

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992016617A1 (en) * 1991-03-15 1992-10-01 Novo Nordisk A/S Thermostable pullulanase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992016617A1 (en) * 1991-03-15 1992-10-01 Novo Nordisk A/S Thermostable pullulanase

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 13 August 1997 (1997-08-13), LIEBL W: "Thermotoga maritima pulA gene", XP002109307 *
KOCH R ET AL: "Purification and properties of a thermostable pullulanase from a newly isolated termophilic anaerobic bacterium, Fervidobacterium pennavorans Ven5.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 3, 1997, WASHINGTON,DC, US, pages 1088 - 1094, XP002109305, ISSN: 0099-2240 *
RUDIGER A: "Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 61, no. 2, February 1995 (1995-02-01), WASHINGTON,DC, US, pages 567 - 575, XP002109306, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
WO1999035274A2 (en) 1999-07-15
AU1779199A (en) 1999-07-26

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