WO1999034014A3 - A method for the determination of a nucleic acid - Google Patents

A method for the determination of a nucleic acid Download PDF

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Publication number
WO1999034014A3
WO1999034014A3 PCT/EP1998/008336 EP9808336W WO9934014A3 WO 1999034014 A3 WO1999034014 A3 WO 1999034014A3 EP 9808336 W EP9808336 W EP 9808336W WO 9934014 A3 WO9934014 A3 WO 9934014A3
Authority
WO
WIPO (PCT)
Prior art keywords
extension
determination
extension products
primers
nucleic acid
Prior art date
Application number
PCT/EP1998/008336
Other languages
French (fr)
Other versions
WO1999034014A2 (en
Inventor
Henrik Oerum
Troels Koch
Thomas Kofoed
Hans-Georg Batz
Original Assignee
Roche Diagnostics Gmbh
Henrik Oerum
Troels Koch
Thomas Kofoed
Batz Hans Georg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Henrik Oerum, Troels Koch, Thomas Kofoed, Batz Hans Georg filed Critical Roche Diagnostics Gmbh
Publication of WO1999034014A2 publication Critical patent/WO1999034014A2/en
Publication of WO1999034014A3 publication Critical patent/WO1999034014A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for the determination of nucleic acids is disclosed wherein in a first extension reaction a multiplicity of extension products of a first set of primers is produced, said extension products being digestible by nuclease, and in a second extension reaction extension products of a second set of primers are produced using said first extension products as templates, wherein said primers of said second set are not capable of serving as a template for the extension of a complementary primer. Intersample cross contamination can be reduced by subjecting said first extension products to nuclease treatment.
PCT/EP1998/008336 1997-12-23 1998-12-18 A method for the determination of a nucleic acid WO1999034014A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97122737.6 1997-12-23
EP97122737 1997-12-23

Publications (2)

Publication Number Publication Date
WO1999034014A2 WO1999034014A2 (en) 1999-07-08
WO1999034014A3 true WO1999034014A3 (en) 1999-11-25

Family

ID=8227867

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/008336 WO1999034014A2 (en) 1997-12-23 1998-12-18 A method for the determination of a nucleic acid

Country Status (1)

Country Link
WO (1) WO1999034014A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6316230B1 (en) * 1999-08-13 2001-11-13 Applera Corporation Polymerase extension at 3′ terminus of PNA-DNA chimera
US20040023220A1 (en) * 2002-07-23 2004-02-05 Lawrence Greenfield Integrated method for PCR cleanup and oligonucleotide removal
WO2007106690A2 (en) 2006-03-15 2007-09-20 Siemens Healthcare Diagnostics Inc. Degenerate nucleobase analogs
EP2274441B1 (en) * 2008-02-04 2012-06-20 Life Technologies Corporation Composition and method for sequencing nucleic acid
US20120135414A1 (en) 2010-10-28 2012-05-31 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
CA2836494C (en) 2011-05-24 2023-01-03 Biontech Ag Individualized vaccines for cancer

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015881A1 (en) * 1989-06-12 1990-12-27 Cis Bio International Method for detecting specific nucleic acid sequences and applications of same
EP0519338A1 (en) * 1991-06-20 1992-12-23 F. Hoffmann-La Roche Ag Improved methods for nucleic acid amplification
WO1993025706A1 (en) * 1992-06-05 1993-12-23 Buchardt, Dorthe Use of nucleic acid analogues in the inhibition of nucleic acid amplification
WO1994016090A1 (en) * 1993-01-15 1994-07-21 Molecular Tool, Inc. Method for generating single-stranded dna molecules
WO1995008556A1 (en) * 1993-09-21 1995-03-30 Amersham International Plc Reagents comprising chimeric molecules of nucleic acids and nucleic acid analogs
WO1995015974A1 (en) * 1993-12-06 1995-06-15 Pna Diagnostics A/S Protecting nucleic acids and methods of analysis
EP0672677A2 (en) * 1994-03-14 1995-09-20 Hoechst Aktiengesellschaft Polyamide-oligonucleotide derivatives, their production and use
EP0709468A1 (en) * 1994-08-25 1996-05-01 Akzo Nobel N.V. A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification, a diagnostic assay employing said process, a kit and a container suitable for carrying out said process or assay
EP0744470A1 (en) * 1995-05-22 1996-11-27 JOHNSON & JOHNSON CLINICAL DIAGNOSTICS, INC. Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
EP0829542A1 (en) * 1996-09-13 1998-03-18 Hoechst Aktiengesellschaft Method for amplification of nucleic acids

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015881A1 (en) * 1989-06-12 1990-12-27 Cis Bio International Method for detecting specific nucleic acid sequences and applications of same
EP0519338A1 (en) * 1991-06-20 1992-12-23 F. Hoffmann-La Roche Ag Improved methods for nucleic acid amplification
WO1993025706A1 (en) * 1992-06-05 1993-12-23 Buchardt, Dorthe Use of nucleic acid analogues in the inhibition of nucleic acid amplification
WO1994016090A1 (en) * 1993-01-15 1994-07-21 Molecular Tool, Inc. Method for generating single-stranded dna molecules
WO1995008556A1 (en) * 1993-09-21 1995-03-30 Amersham International Plc Reagents comprising chimeric molecules of nucleic acids and nucleic acid analogs
WO1995015974A1 (en) * 1993-12-06 1995-06-15 Pna Diagnostics A/S Protecting nucleic acids and methods of analysis
EP0672677A2 (en) * 1994-03-14 1995-09-20 Hoechst Aktiengesellschaft Polyamide-oligonucleotide derivatives, their production and use
EP0709468A1 (en) * 1994-08-25 1996-05-01 Akzo Nobel N.V. A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification, a diagnostic assay employing said process, a kit and a container suitable for carrying out said process or assay
EP0744470A1 (en) * 1995-05-22 1996-11-27 JOHNSON & JOHNSON CLINICAL DIAGNOSTICS, INC. Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
EP0829542A1 (en) * 1996-09-13 1998-03-18 Hoechst Aktiengesellschaft Method for amplification of nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NIKOFOROV T T ET AL: "THE USE OF PHOSPHOROTHIOATE PRIMERS AND EXONUCLEASE HYDROLYSIS FOR THE PREPARATION OF SINGLE-STRANDED PCR PRODUCTS AND THEIR DETECTIONBY SOLID-PHASE HYBRIDIZATION", PCR METHODS & APPLICATIONS, vol. 3, no. 5, 1 April 1994 (1994-04-01), pages 285 - 291, XP000601523, ISSN: 1054-9803 *

Also Published As

Publication number Publication date
WO1999034014A2 (en) 1999-07-08

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