WO1999024607A3 - DETECTION AND TYPING OF THE iceA GENE OF $i(HELICOBACTER PYLORI) - Google Patents

DETECTION AND TYPING OF THE iceA GENE OF $i(HELICOBACTER PYLORI) Download PDF

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Publication number
WO1999024607A3
WO1999024607A3 PCT/EP1998/007106 EP9807106W WO9924607A3 WO 1999024607 A3 WO1999024607 A3 WO 1999024607A3 EP 9807106 W EP9807106 W EP 9807106W WO 9924607 A3 WO9924607 A3 WO 9924607A3
Authority
WO
WIPO (PCT)
Prior art keywords
variants
icea1
icea2
present
icea gene
Prior art date
Application number
PCT/EP1998/007106
Other languages
French (fr)
Other versions
WO1999024607A9 (en
WO1999024607A2 (en
Inventor
Doorn Leen-Jan Van
Wim Quint
Original Assignee
Innogenetics Nv
Delfts Diagnostic Lab B V
Doorn Leen Jan Van
Wim Quint
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innogenetics Nv, Delfts Diagnostic Lab B V, Doorn Leen Jan Van, Wim Quint filed Critical Innogenetics Nv
Priority to EP98961149A priority Critical patent/EP1029082A2/en
Priority to AU16672/99A priority patent/AU1667299A/en
Priority to CA002306254A priority patent/CA2306254A1/en
Priority to JP2000519600A priority patent/JP2001521766A/en
Publication of WO1999024607A2 publication Critical patent/WO1999024607A2/en
Publication of WO1999024607A9 publication Critical patent/WO1999024607A9/en
Publication of WO1999024607A3 publication Critical patent/WO1999024607A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/205—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Campylobacter (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for detection and/or typing of alleles of the iceA gene of H. pylori present in a biological sample, with said method comprising: i) amplifying polynucleic acids of the iceA gene by use of primers that permit specific amplification either of a fragment of iceA1 variants or of a fragment of iceA2 variants, and/or ii) hybridizing polynucleic acids of the iceA gene, possibly after amplification as in step i), with at least one probe that specifically hybridizes to either iceA1 variants or to iceA2 variants. In order to enable this method, the present invention discloses nucleic acid sequences of iceA1 and iceA2 variants. Primers and probes that can be used to carry out said method are also disclosed. The present invention further relates to diagnostic kits that allow to perform said method. IceA1 and IceA2 protein sequences are also disclosed, as well as antibodies directed against these sequences.
PCT/EP1998/007106 1997-11-06 1998-11-06 DETECTION AND TYPING OF THE iceA GENE OF $i(HELICOBACTER PYLORI) WO1999024607A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP98961149A EP1029082A2 (en) 1997-11-06 1998-11-06 DETECTION AND TYPING OF THE $i(iceA) GENE OF $i(HELICOBACTER PYLORI)
AU16672/99A AU1667299A (en) 1997-11-06 1998-11-06 Detection and typing of the (icea) gene of (helicobacter pylori)
CA002306254A CA2306254A1 (en) 1997-11-06 1998-11-06 Detection and typing of the icea gene of helicobacter pylori
JP2000519600A JP2001521766A (en) 1997-11-06 1998-11-06 Detection and typing of the iceA gene of Helicobacter pylori

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97870180 1997-11-06
EP97870180.3 1997-11-06

Publications (3)

Publication Number Publication Date
WO1999024607A2 WO1999024607A2 (en) 1999-05-20
WO1999024607A9 WO1999024607A9 (en) 1999-08-12
WO1999024607A3 true WO1999024607A3 (en) 1999-10-21

Family

ID=8231064

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/007106 WO1999024607A2 (en) 1997-11-06 1998-11-06 DETECTION AND TYPING OF THE iceA GENE OF $i(HELICOBACTER PYLORI)

Country Status (5)

Country Link
EP (1) EP1029082A2 (en)
JP (1) JP2001521766A (en)
AU (1) AU1667299A (en)
CA (1) CA2306254A1 (en)
WO (1) WO1999024607A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111378771B (en) * 2020-03-26 2023-07-28 重庆博利达医学科技有限公司 Nucleic acid composition for detecting helicobacter pylori virulence genes, and reagent, kit and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997043901A1 (en) * 1996-05-20 1997-11-27 Vanderbilt University IceA GENE AND RELATED METHODS

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997043901A1 (en) * 1996-05-20 1997-11-27 Vanderbilt University IceA GENE AND RELATED METHODS

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
ALARCON ET AL.: "PCR-RFLP OF iceA GENE IN SPANISH HELICOBACTER PYLORI CLINICAL ISOLATES", GUT, vol. 41, no. suppl.1, September 1997 (1997-09-01), pages A113 - 10/420, XP002063763 *
BLASER: "HETEROGENEITY OF HELICOBACTER PYLORI", EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, vol. 9, no. suppl. 1, April 1997 (1997-04-01), pages S3 - S7, XP002102906 *
DATABASE EMBL 2 September 1996 (1996-09-02), PEEK ET AL.: "EXPRESSION OF A NOVEL ULCER-ASSOCIATED GENE, iceA, BY H. PYLORI FOLLOWING CONTACT WITH GASTRIC EPITHELIUM", XP002063764 *
DATABASE EMBL 20 October 1998 (1998-10-20), RAUDONIKIENE AND BERG: "iceA1 gene from H.pylori (Alaska strain 209)", XP002102909 *
DATABASE EMBL 20 October 1998 (1998-10-20), RAUDONIKIENE AND BERG: "iceA2 segment from H.pylori", XP002102910 *
ILVER ET AL.: "HELICOBACTER PYLORI ADHESIN BINDING FUCOSYLATED HISTO-BLOOD GROUP ANTIGENS REVEALED BY RETAGGING", SCIENCE, vol. 279, January 1998 (1998-01-01), pages 373 - 377, XP002102908 *
NG ET AL.: "DETERMINATION OF cagA, iceA, AND vacA GENOTYPES IN PATIENTS FROM AREA OF HIGH POPULATION PREVALENCE OF HELICOBACTER PYLORI", GUT, vol. 41, September 1997 (1997-09-01), pages A114 - 10/422, XP002102905 *
PEEK ET AL.: "EXPRESSION OF A NOVEL ULCER-ASSOCIATED H.PYLORI GENE, ICEA, FOLLOWING ADHERENCE TO GASTRIC EPITHELIAL CELLS", GASTROENTEROLOGY, vol. 110, no. 4, April 1996 (1996-04-01), pages A225, XP002063761 *
PEEK ET AL.: "EXPRESSION OF ICEA, A NOVEL ULCER-ASSOCIATED H.PYLORI GENE, IS INDUCED BY CONTACT WITH GASTRIC EPITHELIAL CELLS AND IS ASSOCIATED WITH ENHANCED MUCOSAL IL-8", GUT, vol. 39, no. SUPPL.2, October 1996 (1996-10-01), pages 3B:87, XP002063762 *
VAN DOORN ET AL.: "CLINICAL RELEVANCE OF THE cagA,vacA,AND iceA STATUS OF HELICOBACTER PYLORI", GASTROENTEROLOGY, vol. 115, July 1998 (1998-07-01), pages 58 - 66, XP002102907 *

Also Published As

Publication number Publication date
EP1029082A2 (en) 2000-08-23
WO1999024607A9 (en) 1999-08-12
AU1667299A (en) 1999-05-31
JP2001521766A (en) 2001-11-13
CA2306254A1 (en) 1999-05-20
WO1999024607A2 (en) 1999-05-20

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