WO1998057983A2 - Mammalian neuro-growth factor like protein - Google Patents

Abstract

Novel mammalian neuro-growth factor like polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods including antibodies and anti-idiotypic antibodies.

Description

MAMMALIAN NEURO-GROWTH FACTOR- LIKE PROTEIN

BACKGROUND OF THE INVENTION

Proliferation and differentiation of cells of multicellular organisms are controlled by hormones and polypeptide growth factors. These diffusable molecules allow cells to communicate with each other and act in concert to form cells and organs, and to repair and regenerate damaged tissue. Examples of hormones and growth factors include the steroid hormones ( e . g. estrogen, testosterone) , parathyroid hormone, follicle stimulating hormone, the interleukins, platelet derived growth factor (PDGF) , epidermal growth factor (EGF) , granulocyte-macrophage colony stimulating factor (GM-CSF) , erythropoietin (EPO) and calcitonin.

Hormones and growth factors influence cellular metabolism by binding to proteins. Proteins may be integral membrane proteins that are linked to signaling pathways within the cell, such as second messenger systems. Other classes of proteins are soluble molecules, such as the transcription factors.

SUMMARY OF THE INVENTION

The present invention addresses this need by providing a novel neuro-growth factor like polypeptide called Zneul and related compositions and methods. Within one aspect, the present invention provides an isolated polynucleotide encoding a mammalian polypeptide termed

Zneul. The mature human Zneul polypeptide is comprised of a sequence of amino acids approximately 254 amino acids long. Amino acid residue 20 of SEQ ID NO: 2, a threonine, is the initial amino acid of the mature polypeptide. Thus, it is believed that amino residues 1-19 comprise a signal sequence, and the mature Zneul polypeptide is represented by the amino acid sequence comprised of residues 20-254. The mature Zneul polypeptide is further represented by SEQ ID NO: 3. Mouse Zneul is defined by SEQ ID NOs:18 and 19. Having a signal sequence of amino acid residues 1-23, and the mature mouse Zneul is from 24-278 represented by SEQ ID NO: 24. Within an additional embodiment, the polypeptide further comprises an affinity tag. Within a further embodiment, the polynucleotide is DNA.

Within a second aspect of the invention there is provided an expression vector comprising (a) a transcription promoter; (b) a DNA segment encoding Zneul polypeptide, and (c) a transcription terminator, wherein the promoter, DNA segment, and terminator are operably linked.

Within a third aspect of the invention there is provided a cultured eukaryotic cell into which has been introduced an expression vector as disclosed above, wherein said cell expresses a protein polypeptide encoded by the DNA segment.

Within a further aspect of the invention there is provided a chimeric polypeptide consisting essentially of a first portion and a second portion joined by a peptide bond. The first portion of the chimeric polypeptide consists essentially of (a) a Zneul polypeptide as shown in SEQ ID NO: 2 (b) allelic variants of SEQ ID NO: 2; and (c) protein polypeptides that are at least 90% identical to (a) or (b) . The second portion of the chimeric polypeptide consists essentially of another polypeptide such as an affinity tag. Within one embodiment the affinity tag is an immunoglobulin F_c polypeptide. The invention also provides expression vectors encoding the chimeric polypeptides and host cells transfected to produce the chimeric polypeptides.

Within an additional aspect of the invention there is provided an antibody that specifically binds to a Zneul polypeptide as disclosed above, and also an anti- idiotypic antibody which neutralizes the antibody to a Zneul polypeptide.

In addition to the above, the present invention is also directed domains of the polypeptide including SEQ ID N0s:8, 9, 10, 11, 12, 13, 14, 15, and 16.

An additional embodiment of the present invention relates to a peptide or polypeptide which has the amino acid sequence of an epitope-bearing portion of a Zneul polypeptide having an amino acid sequence described above. Peptides or polypeptides having the amino acid sequence of an epitope-bearing portion of a Zneul polypeptide of the present invention include portions of such polypeptides with at least nine, preferably at least 15 and more preferably at least 30 to 50 amino acids, although epitope-bearing polypeptides of any length up to and including the entire amino acid sequence of a polypeptide of the present invention described above are also included in the present invention. Specific examples of said polypeptides are defined by the amino acid sequences of SEQ ID NOs : 20-23. Also claimed are any of these polypeptides that are fused to another polypeptide or carrier molecule.

Another embodiment of the present invention relates to a method for producing an antibody which binds to a peptide or polypeptide defined by SEQ ID NOs: 2-3,8, 9, 11-16, and 19-24 or to a peptide or polypeptide which is at least 90% identical to said peptide or polypeptide comprising inoculating an animal with said peptide or polypeptide or with a nucleic acid which encodes said peptide or polypeptide, wherein said animal produces antibodies to said peptide or polypeptide; and isolating said antibody.

These and other aspects of the invention will become evident upon reference to the following detailed description.

DETAILED DESCRIPTION OF THE INVENTION

The teachings of all of the references cited herein are incorporated in their entirety by reference.

The term "allelic variant" is used herein to denote any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in phenotypic polymorphism within populations. Gene mutations can be silent (no change in the encoded polypeptide) or may encode polypeptides having altered amino acid sequence. The term allelic variant is also used herein to denote a protein encoded by an allelic variant of a gene.

The term "expression vector" is used to denote a DNA molecule, linear or circular, that comprises a segment encoding a polypeptide of interest operably linked to additional segments that provide for its transcription. Such additional segments include promoter and terminator sequences, and may also include one or more origins of replication, one or more selectable markers, an enhancer, a polyadenylation signal, etc. Expression vectors are generally derived from plasmid or viral DNA, or may contain elements of both.

The term "isolated", when applied to a polynucleotide, denotes that the polynucleotide has been removed from its natural genetic milieu and is thus free of other extraneous or unwanted coding sequences, and is in a form suitable for use within genetically engineered protein production systems.

"Operably linked" , when referring to DNA segments, indicates that the segments are arranged so that they function in concert for their intended purposes, e . g. transcription initiates in the promoter and proceeds through the coding segment to the terminator.

A "polynucleotide" is a single- or double- stranded polymer of deoxyribonucleotide or ribonucleotide bases read from the 5' to the 3' end. Polynucleotides include RNA and DNA, and may be isolated from natural sources, synthesized in vi tro, or prepared from a combination of natural and synthetic molecules.

The term "promoter" is used herein for its art- recognized meaning to denote a portion of a gene containing DNA sequences that provide for the binding of RNA polymerase and initiation of transcription. Promoter sequences are commonly, but not always, found in the 5' non-coding regions of genes.

A "soluble protein" is a protein polypeptide that is not bound to a cell membrane. Within preferred embodiments of the invention the isolated polynucleotides will hybridize to similar sized regions of SEQ ID N0:1, or a sequence complementary thereto, under stringent conditions. In general, stringent conditions are selected to be about 5°C lower than the thermal melting point (T_m) for the specific sequence at a defined ionic strength and pH. The T_m is the temperature (under defined ionic strength and pH) at which 50% of the target sequence hybridizes to a perfectly matched probe. Typical stringent conditions are those in which the salt concentration is about 0.02 M or less at pH 7 and the temperature is at least about 60°C. As previously noted, the isolated polynucleotides of the present invention include DNA and RNA. Methods for isolating DNA and RNA are well known in the art. Total RNA can be prepared using guanidine HCI extraction followed by isolation by centrifugation in a CsCl gradient, Chirgwin et al . , Biochemistry 28:52-94 (1979). Poly (A) + RNA is prepared from total RNA using the method of Aviv and Leder, Proc . Na tl . Acad . Sci . USA 63:1408-1412 (1972). Complementary DNA (cDNA) is prepared from poly (A) ⁺ RNA using known methods. Polynucleotides encoding Zneul polypeptides are then identified and isolated by, for example, hybridization or PCR.

The polynucleotides of the present invention can be synthesized using DNA synthesizer. Currently the method of choice is the phosphoramidite method. If chemically synthesized double stranded DNA is required for an application such as the synthesis of a gene or a gene fragment, then each complementary strand is made separately. The production of short genes (60 to 80 bp) is technically straightforward and can be accomplished by synthesizing the complementary strands and then annealing them. For the production of longer genes (>300 bp) , however, special strategies must be invoked, because the coupling efficiency of each cycle during chemical DNA synthesis is seldom 100%. To overcome this problem, synthetic genes (double-stranded) are assembled in modular form from single-stranded fragments that are from 20 to 100 nucleotides in length. See Glick, Bernard R. and Jack J. Pasternak, Molecular Biotechnology, Principles & Applications of Recombinant DNA, (ASM Press, Washington, D.C. 1994), Itakura, K. et al . Synthesis and use of synthetic oligonucleotides. Annu . .Rev. Biochem . 53 : 323- 356 (1984), and Climie, S. et al . Chemical synthesis of the thymidylate synthase gene. Proc . Na tl . Acad. Sci . USA 81 :633-637 (1990) .

Those skilled in the art will recognize that the sequences disclosed in SEQ ID NOS : 1 , 2 and 3 represent a single allele of the human. Allelic variants of these sequences can be cloned by probing cDNA or genomic libraries from different individuals according to standard procedures .

The present invention further provides counterpart proteins and polynucleotides from other species ("species orthologs"). Of particular interest are Zneul polypeptides from other mammalian species, including murine, porcine, ovine, bovine, canine, feline, equine, and other primates . Species orthologs of the human Zneul protein can be cloned using information and compositions provided by the present invention in combination with conventional cloning techniques. For example, a cDNA can be cloned using mRNA obtained from a tissue or cell type that expresses the protein. Suitable sources of mRNA can be identified by probing Northern blots with probes designed from the sequences disclosed herein. A library is then prepared from mRNA of a positive tissue or cell line. A protein-encoding cDNA can then be isolated by a variety of methods, such as by probing with a complete or partial human or mouse cDNA or with one or more sets of degenerate probes based on the disclosed sequences. A cDNA can also be cloned using the polymerase chain reaction, or PCR (Mullis, U.S. Patent No. 4,683,202), using primers designed from the sequences disclosed herein. Within an additional method, the cDNA library can be used to transform or transfect host cells, and expression of the cDNA of interest can be detected with an antibody to the protein. Similar techniques can also be applied to the isolation of genomic clones. As used and claimed the language "an isolated polynucleotide which encodes a polypeptide, said polynucleotide being defined by SEQ ID NO: 2" includes all allelic variants and species orthologs of the polypeptide of SEQ ID NO : 2.

The present invention also provides isolated protein polypeptides that are substantially homologous to the polypeptide of SEQ ID NO: 3 and its species orthologs. By "isolated" is meant a protein or polypeptide that is found in a condition other than its native environment, such as apart from blood and animal tissue. In a preferred form, the isolated polypeptide is substantially free of other polypeptides, particularly other polypeptides of animal origin. It is preferred to provide the polypeptides in a highly purified form, i.e. greater than 95% pure, more preferably greater than 99% pure. The term "substantially homologous" is used herein to denote polypeptides having 50%, preferably 60%, more preferably at least 80%, sequence identity to the sequence shown in SEQ ID NO: 2, or its species orthologs. Such polypeptides will more preferably be at least 90% identical, and most preferably 95% or more identical to SEQ ID NO : 3 , or its species orthologs. Percent sequence identity is determined by conventional methods. See, for example, Altschul et al . , Bull . Math . Bio . 48 : 603-616 (1986) and

Henikoff and Henikoff, Proc . Na tl . Acad . Sci . USA

83:10915-10919 (1992). Briefly, two amino acid sequences are aligned to optimize the alignment scores using a gap opening penalty of 10, a gap extension penalty of 1, and the "blossom 62" scoring matrix of Henikoff and Henikoff

(ibid.) as shown in Table 1 (amino acids are indicated by the standard one-letter codes) . The percent identity is then calculated as : Total number of identical matches x 100

[length of the longer sequence plus the number of gaps introduced into the longer sequence in order to align the two sequences]

Table 1

A R N D C Q E G H I L K M F P S T W Y V

A 4 R -1 5

N -2 0 6

D -2 -2 1 6

C 0 -3 -3 -3 9

Q -1 1 0 0 -3 5 E -1 0 0 2 -4 2 5

G 0 -2 0 -1 -3 -2 -2 6

H -2 0 1 -1 -3 0 0 -2 8

I -1 -3 -3 -3 -1 -3 -3 -4 -3 4

L -1 -2 -3 -4 -1 -2 -3 -4 -3 2 4 K -1 2 0 -1 -3 1 1 -2 -1 -3 -2 5

M -1 -1 -2 -3 -1 0 -2 -3 -2 1 2 -1 5

F -2 -3 -3 -3 -2 -3 -3 -3 -1 0 0 -3 0 6

P -1 -2 -2 -1 -3 -1 -1 -2 -2 -3 -3 -1 -2 -4 7

S 1 -1 1 0 -1 0 0 0 -1 -2 -2 0 -1 -2 -1 4 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 W -3 -3 -4 -4 -2 -2 -3 -2 -2 -3 -2 -3 -1 1 -4 -3 -2 11

Y -2 -2 -2 -3 -2 -1 -2 -3 2 -1 -1 -2 -1 3 -3 -2 -2 2 7

V 0 -3 -3 -3 -1 -2 -2 -3 -3 3 1 -2 1 -1 -2 -2 0 -3 -1 4

Sequence identity of polynucleotide molecules is determined by similar methods using a ratio as disclosed above .

Substantially homologous proteins and polypeptides are characterized as having one or more amino acid substitutions, deletions or additions. These changes are preferably of a minor nature, that is conservative amino acid substitutions (see Table 2) and other substitutions that do not significantly affect the folding or activity of the protein or polypeptide; small deletions, typically of one to about 30 amino acids; and small amino- or carboxyl-terminal extensions, such as an amino-terminal methionine residue, a small linker peptide of up to about 20-25 residues, or a small extension that facilitates purification (an affinity tag) , such as a poly-histidine tract, protein A, Nilsson et al . , EMBO J. 4:1075, (1985); Nilsson et al . , Me thods Enzymol . 198 : 3 , (1991), glutathione S transferase, Smith and Johnson, Gene 6V:31, (1988), or other antigenic epitope or binding domain. See, in general Ford et al . , Protein Expression and Purification 2 : 95-107, (1991). DNAs encoding affinity tags are available from commercial suppliers (e.g., Pharmacia Biotech, Piscataway, NJ) .

Table 2

Conservative amino acid substitutions Basic: arginine lysine histidine

Acidic: glutamic acid aspartic acid Polar: glutamine asparagine Hydrophobic: leucine isoleucine Table 2, continued valine Aromatic: phenylalanine tryptophan tyrosine

Small: glycine alanine serine threonine methionine

Essential amino acids in the polypeptides of the present invention can be identified according to procedures known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis, Cunningham and Wells, Science 244 , 1081-1085, (1989); Bass et al . , Proc . Natl . Acad . Sci . USA 88:4498-4502, (1991). In the latter technique, single alanine mutations are introduced at every residue in the molecule, and the resultant mutant molecules are tested for biological activity (e.g., ligand binding and signal transduction) to identify amino acid residues that are critical to the activity of the molecule. Sites of ligand-protein interaction can also be determined by analysis of crystal structure as determined by such techniques as nuclear magnetic resonance, crystallography or photoaffinity labeling. See, for example, de Vos et al . , Science 255:306-312, (1992); Smith et al . , J. Mol . Biol . 224:899-904, (1992); Wlodaver et al . , FEBS Lett . 303:59-64, (1992). The identities of essential amino acids can also be inferred from analysis of homologies with related proteins .

Multiple amino acid substitutions can be made and tested using known methods of mutagenesis and screening, such as those disclosed by Reidhaar-Olson and Sauer, Science 241:53-57 , (1988) or Bowie and Sauer, Proc . Natl . Acad . Sci . USA 86:2152-2156, (1989). Briefly, these authors disclose methods for simultaneously randomizing two or more positions in a polypeptide, selecting for functional polypeptide, and then sequencing the mutagenized polypeptides to determine the spectrum of allowable substitutions at each position. Other methods that can be used include phage display, e.g., Low an et al . , Biochem . 30:10832-10837, (1991); Ladner et al . , U.S. Patent No. 5,223,409; Huse, WIPO Publication WO 92/06204, and region-directed mutagenesis, Derbyshire et al . , Gene 46:145, (1986); Ner et al . , DNA 7:127, (1988)

Mutagenesis methods as disclosed above can be combined with high-throughput screening methods to detect activity of cloned, mutagenized proteins in host cells.

Preferred assays in this regard include cell proliferation assays and biosensor-based ligand-binding assays, which are described below. Mutagenized DNA molecules that encode active proteins or portions thereof (e.g., ligand-binding fragments) can be recovered from the host cells and rapidly sequenced using modern equipment . These methods allow the rapid determination of the importance of individual amino acid residues in a polypeptide of interest, and can be applied to polypeptides of unknown structure.

Using the methods discussed above, one of ordinary skill in the art can prepare a variety of polypeptides that are substantially homologous to SEQ ID NO: 3 or allelic variants thereof and retain the properties of the wild-type protein. As expressed and claimed herein the language, "a polypeptide as defined by SEQ ID NO: 2" includes all allelic variants and species orthologs of the polypeptide . The protein polypeptides of the present invention, including full-length proteins, protein fragments (e.g. ligand-binding fragments), and fusion polypeptides can be produced in genetically engineered host cells according to conventional techniques. Suitable host cells are those cell types that can be transformed or transfected with exogenous DNA and grown in culture, and include bacteria, fungal cells, and cultured higher eukaryotic cells. Eukaryotic cells, particularly cultured cells of multicellular organisms, are preferred.

Techniques for manipulating cloned DNA molecules and introducing exogenous DNA into a variety of host cells are disclosed by Sambrook et al . , Mol ecular Cloning: A Laboratory Manual , 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, (1989), and Ausubel et al . , ibid.

In general, a DNA sequence encoding a Zneul polypeptide is operably linked to other genetic elements required for its expression, generally including a transcription promoter and terminator, within an expression vector. The vector will also commonly contain one or more selectable markers and one or more origins of replication, although those skilled in the art will recognize that within certain systems selectable markers may be provided on separate vectors, and replication of the exogenous DNA may be provided by integration into the host cell genome. Selection of promoters, terminators, selectable markers, vectors and other elements is a matter of routine design within the level of ordinary skill in the art. Many such elements are described in the literature and are available through commercial suppliers.

Another embodiment of the present invention provides for a peptide or polypeptide comprising an epitope-bearing portion of a polypeptide of the invention. The epitope of the this polypeptide portion is an immunogenic or antigenic epitope of a polypeptide of the invention. A region of a protein to which an antibody can bind is defined as an "antigenic epitope" . See for instance, Geysen, H.M. et al., Proc . Natl . Acad Sci . USA 81:3998-4002 (1984) .

As to the selection of peptides or polypeptides bearing an antigenic epitope (i.e., that contain a region of a protein molecule to which an antibody can bind) , it is well known in the art that relatively short synthetic peptides that mimic part of a protein sequence are routinely capable of eliciting an antiserum that reacts with the partially mimicked protein. See Sutcliffe, J.G. et al . Science 213:660-666 (1983). Peptides capable of eliciting protein-reactive sera are frequently represented in the primary sequence of a protein, can be characterized by a set of simple chemical rules, and are confined neither to immunodominant regions of intact proteins (i.e., immunogenic epitopes) nor to the amino or carboxyl terminals. Peptides that are extremely hydrophobic and those of six or fewer residues generally are ineffective at inducing antibodies that bind to the mimicked protein; longer soluble peptides, especially those containing proline residues, usually are effective.

Antigenic epitope-bearing peptides and polypeptides of the invention are therefore useful to raise antibodies, including monoclonal antibodies, that bind specifically to a polypeptide of the invention.

Antigenic epitope-bearing peptides and polypeptides of the present invention contain a sequence of at least nine, preferably between 15 to about 30 amino acids contained within the amino acid sequence of a polypeptide of the invention. However, peptides or polypeptides comprising a larger portion of an amino acid sequence of the invention, containing from 30 to 50 amino acids, or any length up to and including the entire amino acid sequence of a polypeptide of the invention, also are useful for inducing antibodies that react with the protein. Preferably, the amino acid sequence of the epitope-bearing peptide is selected to provide substantial solubility in aqueous solvents (i.e., the sequence includes relatively hydrophilic residues and hydrophobic residues are preferably avoided) ; and sequences containing proline residues are particularly preferred. All of the polypeptides shown in the sequence listing contain antigenic epitopes to be used according to the present invention, however, specifically designed antigenic epitopes include the peptides defined by SEQ ID NOs : 20-24.

Polynucleotides, generally a cDNA sequence, of the present invention encode the above-described polypeptides. A cDNA sequence which encodes a polypeptide of the present invention is comprised of a series of codons, each amino acid residue of the polypeptide being encoded by a codon and each codon being comprised of three nucleotides. The amino acid residues are encoded by their respective codons as follows.

Alanine (Ala) is encoded by GCA, GCC, GCG or

GCT;

Cysteine (Cys) is encoded by TGC or TGT;

Aspartic acid (Asp) is encoded by GAC or GAT

Glutamic acid (Glu) is encoded by GAA or GAG Phenylalanine (Phe) is encoded by TTC or TTT

Glycine (Gly) is encoded by GGA, GGC, GGG or GGT;

Histidine (His) is encoded by CAC or CAT;

Isoleucine (lie) is encoded by ATA, ATC or ATT; Lysine (Lys) is encoded by AAA, or AAG; Leucine (Leu) is encoded by TTA, TTG, CTA, CTC, CTG or CTT;

Methionine (Met) is encoded by ATG;

Asparagine (Asn) is encoded by AAC or AAT; Proline (Pro) is encoded by CCA, CCC, CCG or

CCT;

Glutamine (Gin) is encoded by CAA or CAG;

Arginine (Arg) is encoded by AGA, AGG, CGA, CGC, CGG or CGT; Serine (Ser) is encoded by AGC, AGT, TCA, TCC,

TCG or TCT;

Threonine (Thr) is encoded by ACA, ACC, ACG or ACT;

Valine (Val) is encoded by GTA, GTC, GTG or GTT; Tryptophan (Trp) is encoded by TGG; and

Tyrosine (Tyr) is encoded by TAC or TAT.

It is to be recognized that according to the present invention, when a cDNA is claimed as described above, it is understood that what is claimed are both the sense strand, the anti-sense strand, and the DNA as double-stranded having both the sense and anti-sense strand annealed together by their respective hydrogen bonds . Also claimed is the messenger RNA (mRNA) which encodes the polypeptides of the present invention, and which mRNA is encoded by the above-described cDNA. A messenger RNA (mRNA) will encode a polypeptide using the same codons as those defined above, with the exception that each thymine (T) is replaced by a uracil nucleotide (U) .

To direct a Zneul polypeptide into the secretory pathway of a host cell, a secretory signal sequence (also known as a leader sequence, prepro sequence or pre sequence) is provided in the expression vector. The secretory signal sequence may be that of the protein, or may be derived from another secreted protein (e.g., t-PA) or synthesized de novo . The secretory signal sequence is joined to the Zneul DNA sequence in the correct reading frame. Secretory signal sequences are commonly positioned 5 ' to the DNA sequence encoding the polypeptide of interest, although certain signal sequences may be positioned elsewhere in the DNA sequence of interest (see, e.g., Welch et al . , U.S. Patent No. 5,037,743; Holland et al . , U.S. Patent No. 5,143,830).

Cultured mammalian cells are preferred hosts within the present invention. Methods for introducing exogenous DNA into mammalian host cells include calcium phosphate-mediated transfection, Wigler et al . , Cell 14:725, (1978); Corsaro and Pearson, Soma tic Cell Genetics 7:603, (1981): Graham and Van der Eb, Virology 52:456, (1973), electroporation, Neumann et al . , EMBO J. 1:841- 845, (1982), DEAE-dextran mediated transfection, Ausubel et al . , eds . , Current Protocols in Mol ecular Biology, John Wiley and Sons, Inc., NY, (1987), and liposome-mediated transfection, Hawley-Nelson et al . , Focus 15 : 13 , (1993); Ciccarone et al . , Focus 15:80, (1993) . The production of recombinant polypeptides in cultured mammalian cells is disclosed, for example, by Levinson et al . , U.S. Patent No. 4,713,339; Hagen et al . , U.S. Patent No. 4,784,950; Palmiter et al . , U.S. Patent No. 4,579,821; and Ringold, U.S. Patent No. 4,656,134. Suitable cultured mammalian cells include the COS-1 (ATCC No. CRL 1650), COS-7 (ATCC No. CRL 1651), BHK (ATCC No. CRL 1632), BHK 570 (ATCC No. CRL 10314), 293, ATCC No. CRL 1573; Graham et al . , J. Gen . Virol . 36 : 59 - 12 , (1977) and Chinese hamster ovary (e.g. CH0-K1; ATCC No. CCL 61) cell lines. Additional suitable cell lines are known in the art and available from public depositories such as the American Type Culture Collection, Rockville, Maryland. In general, strong transcription promoters are preferred, such as promoters from SV-40 or cytomegalovirus . See, e.g., U.S. Patent No. 4,956,288. Other suitable promoters include those from metallothionein genes (U.S. Patent Nos. 4,579,821 and 4 , 601, 978 , and the adenovirus major late promoter.

Drug selection is generally used to select for cultured mammalian cells into which foreign DNA has been inserted. Such cells are commonly referred to as "transfectants" . Cells that have been cultured in the presence of the selective agent and are able to pass the gene of interest to their progeny are referred to as "stable transfectants." A preferred selectable marker is a gene encoding resistance to the antibiotic neomycin. Selection is carried out in the presence of a neomycin- type drug, such as G-418 or the like. Selection systems may also be used to increase the expression level of the gene of interest, a process referred to as "amplification." Amplification is carried out by culturing transfectants in the presence of a low level of the selective agent and then increasing the amount of selective agent to select for cells that produce high levels of the products of the introduced genes. A preferred amplifiable selectable marker is dihydrofolate reductase, which confers resistance to methotrexate . Other drug resistance genes (e.g. hygromycin resistance, multi-drug resistance, puromycin acetyltransferase) can also be used.

Other higher eukaryotic cells can also be used as hosts, including insect cells, plant cells and avian cells. Transformation of insect cells and production of foreign polypeptides therein is disclosed by Guarino et al . , U.S. Patent No. 5,162,222; Bang et al . , U.S. Patent No. 4,775,624; and WIPO publication WO 94/06463. The use of Agrobacterium rhizogenes as a vector for expressing genes in plant cells has been reviewed by Sinkar et al . , J. Biosci . (Bangalore) 11:47-58, (1987).

Fungal cells, including yeast cells, and particularly cells of the genus Sa ccharomyces , can also be used within the present invention, such as for producing protein fragments or polypeptide fusions. Methods for transforming yeast cells with exogenous DNA and producing recombinant polypeptides therefrom are disclosed by, for example, Kawasaki, U.S. Patent No. 4,599,311; Kawasaki et al . , U.S. Patent No. 4,931,373; Brake, U.S. Patent No. 4,870,008; Welch et al . , U.S. Patent No. 5,037,743; and Murray et al . , U.S. Patent No. 4,845,075. Transformed cells are selected by phenotype determined by the selectable marker, commonly drug resistance or the ability to grow in the absence of a particular nutrient (e.g., leucine) . A preferred vector system for use in yeast is the P0T1 vector system disclosed by Kawasaki et al . (U.S. Patent No. 4,931,373), which allows transformed cells to be selected by growth in glucose-containing media. Suitable promoters and terminators for use in yeast include those from glycolytic enzyme genes (see, e.g., Kawasaki, U.S. Patent No. 4,599,311; Kingsman et al . , U.S. Patent No. 4,615,974; and Bitter, U.S. Patent No. 4,977,092 ) and alcohol dehydrogenase genes. See also U.S. Patents Nos. 4,990,446; 5,063,154; 5,139,936 and 4,661,454. Transformation systems for other yeasts, including Hansenula polymorpha , Schi zosaccharomyces pombe, Kluyveromyces la ctis , Kluyveromyces fragilis , Ustilago maydis , Pichia pastoris _r Pichia methanolica , Pi chia guillermondii and Candida mal tosa are known in the art. See, for example, Gleeson et al . , J. Gen . Microbiol . 132:3459-3465, (1986) and Cregg, U.S. Patent No. 4,882,279. Aspergillus cells may be utilized according to the methods of McKnight et al . , U.S. Patent No. 4,935,349.^' Methods for transforming Acremonium chrysogenum are disclosed by Sumino et al., U.S. Patent No. 5,162,228. Methods for transforming Neurospora are disclosed by Lambowitz, U.S. Patent No. 4,486,533.

Transformed or transfected host cells are cultured according to conventional procedures in a culture medium containing nutrients and other components required for the growth of the chosen host cells. A variety of suitable media, including defined media and complex media, are known in the art and generally include a carbon source, a nitrogen source, essential amino acids, vitamins and minerals. Media may also contain such components as growth factors or serum, as required. The growth medium will generally select for cells containing the exogenously added DNA by, for example, drug selection or deficiency in an essential nutrient which is complemented by the selectable marker carried on the expression vector or co- transfected into the host cell.

Within one aspect of the present invention, a novel protein is produced by a cultured cell, and the cell is used to screen for a receptor or receptors for the protein, including the natural receptor, as well as agonists and antagonists of the natural ligand.

PROTEIN ISOLATION:

Expressed recombinant polypeptides (or chimeric polypeptides) can be purified using fractionation and/or conventional purification methods and media. Ammonium sulfate precipitation and acid or chaotrope extraction may be used for fractionation of samples. Exemplary purification steps may include hydroxyapatite, size exclusion, FPLC and reverse-phase high performance liquid chromatography. Suitable anion exchange media include derivatized dextrans, agarose, cellulose, polyacrylamide, specialty silicas, and the like. PEI, DEAE, QAE and Q derivatives are preferred, with DEAE Fast-Flow Sepharose (Pharmacia, Piscataway, NJ) being particularly preferred. Exemplary chromatographic media include those media derivatized with phenyl, butyl, or octyl groups, such as Phenyl-Sepharose FF (Pharmacia) , Toyopearl butyl 650 (Toso Haas, Montgomeryville, PA) , Octyl-Sepharose (Pharmacia) and the like; or polyacrylic resins, such as Amberchrom CG 71 (Toso Haas) and the like. Suitable solid supports include glass beads, silica-based resins, cellulosic resins, agarose beads, cross-linked agarose beads, polystyrene beads, cross-linked polyacrylamide resins and the like that are insoluble under the conditions in which they are to be used. These supports may be modified with reactive groups that allow attachment of proteins by amino groups, carboxyl groups, sulfhydryl groups, hydroxyl groups and/or carbohydrate moieties. Examples of coupling chemistries include cyanogen bromide activation, N- hydroxysuccinimide activation, epoxide activation, sulfhydryl activation, hydrazide activation, and carboxyl and amino derivatives for carbodiimide coupling chemistries. These and other solid media are well known and widely used in the art, and are available from commercial suppliers. Methods for binding receptor polypeptides to support media are well known in the art. Selection of a particular method is a matter of routine design and is determined in part by the properties of the chosen support. See, for example, Affini ty Chromatography: Principles & Methods, Pharmacia LKB Biotechnology, Uppsala, Sweden, (1988) .

The polypeptides of the present invention can be isolated by exploitation of their properties . For example, immobilized metal ion adsorption (IMAC) chromatography can be used to purify histidine-rich proteins. Briefly, a gel is first charged with divalent metal ions to form a chelate, E. Sulkowski, Trends in Biochem . 3 : 1 - 1 , (1985). Histidine-rich proteins will be adsorbed to this matrix with differing affinities, depending upon the metal ion used, and will be eluted by competitive elution, lowering the pH, or use of strong chelating agents. Other methods of purification include purification of glycosylated proteins by lectin affinity chromatography and ion exchange chromatography, Methods in Enzymol . , Vol. 182, "Guide to Protein Purification", M. Deutscher, (ed.), Acad. Press, San Diego, (1990), pp.529- 39. Alternatively, a fusion of the polypeptide of interest and an affinity tag (e.g., polyhistidine, maltose-binding protein, an immunoglobulin domain) may be constructed to facilitate purification.

Physical Structure of Zneul

The Zneul polypeptide shown in SEQ ID NO: 2 has a signal peptide including amino acid residues 1-19. Amino acid residues 20-104 define a hydrophilic domain homologous to an HSMHC3W5A domain, SEQ ID NO: 17, (GenBank No. gl401159) . Amino acid residues 105-135 define a domain homologous to an Epidermal Growth Factor (EGF) domain. Amino acid residues 136-177 define another domain homologous to an EGF domain; and amino acid residues 178- 273 define a domain also homologous to an HSMHC3W5A domain .

However, the first EGF-like domain (EGF1) of Zneul, SEQ ID NO : 9 which corresponds to amino acid residues 105 to 135 of SEQ ID NO: 2, is distinct from any other EGF domain in the prior art . The EGF1 in Zneul is about 56% similar to the HSMHC3W5A_6 domain, its closest human relative. The second EGF- like domain (EGF2) of Zneul, SEQ ID NO: 10 which corresponds to amino acid residues 136 to 177 of SEQ ID NO: 2, is distinct from any other EGF domain in the prior art. EGF2 of Zneul is about 48% similar to PIR_S31101 fibrillin, its closest human relative.

The first HSMHC3W5A-like (HSM1) domain of Zneul, SEQ ID NO: 8 which corresponds to amino acid residues 20- 104 of SEQ ID NO: 2. SEQ ID NO: 8 is approximately 38% similar to HSMHC3W5A, its closest human relative.

The second HSMHC3W5A-like domain (HSM2) of Zneul, SEQ ID NO: 11 which corresponds to amino acid residues 178-273 of SEQ ID NO: 2, is distinct from any other polypeptide in the prior art. It is about 32% similar to HSMHC3W5A 6.

Uses

The tissue specificity of Zneul expression indicates that Zneul can be used as a growth, maintenance, or differentiation factor in the spinal cord, heart, spleen, testis, thyroid and lymph nodes.

The present invention also provides reagents which will find use in diagnostic applications. For example, the Zneul gene has been mapped on chromosome 9q34.3. A Zneul nucleic acid probe could be used to check for abnormalities in chromosome 9. In a normal chromosome 9, one would predict that a Zneul nucleic acid probe would hybridize to chromosome 9. If the probe does not hybridize to chromosome 9, this would indicate an abnormality in chromosome 9.

Zneul ' s closest human homolog is HSMHC3W5A a gene in the HLA class III region, which is contained in a cosmid which contains Notch 4. Zneul is also homologous to Notch 4 in its EGF-like domains. Zneul may be involved in EGF-receptor pathways.

Notch Structure/Function

The original member of this gene family was the Drosophila gene Notch which controls cell fate decisions in the development of the peripheral nervous system. Notch is a cell surface receptor with a single transmembrane domain. Homologues have now been found in C. elegans (linl2 and glpl) , Xenopus , mouse and human. All members of the Notch family have large numbers of EGF-like motifs (29-39 in mouse, 10-13 in C. elegans) and three or more copies of LNR (linl2/ Notch repeats) in the extracellular domain. Notch family members also contain six copies of the cdclO/SWI6 motif (also called ankyrin repeats) and a PEST protein degradation sequence in the intracellular domain. Specific EGF repeats (Drosophila repeats 11 and 12) are involved in ligand binding. LNR may be regulatory domains which bind ligand when high ligand concentrations exist and cause decreased activity of Notch. Cdclθ/SWI6 domains are involved in protein- protein interactions with components of the Notch- activated signal transduction pathway.

Notch Biology

Two different translocations led to formation of altered Notch genes resulting in an oncogenic state. The TAN-1 oncogene is a fusion of part of the β T cell receptor with a small region of the human Notch 1 extracellular domain and the entire intracellular domain. TAN-1 is an activated form of Notch which causes T- lymphoblastic leukemias . The int-3 oncogene is caused by ^~ integration of the mouse mammary tumor virus into the Notch 4 gene resulting in expression of the intact intracellular domain. Int-3 also is an activated form of Notch which leads to mammary carcinoma.

The function of Notch family members has been extensively studied in Drosophila and C. elegans . These proteins control binary decisions that depend on cell-cell interactions. Notch proteins act consistent with their proposed role as a receptor. Gain-of-function and loss- of-function Notch alleles result in opposite cell fate decisions. Notch receptors and their ligands play important roles in lateral inhibition, the process whereby signaling between neighboring cells is amplified by a feedback loop between Notch and its ligand. This process results in increased receptor activity in some cells and increased ligand activity in others leading to the distinction between signaling cells and receiving cells.

It has recently been shown that the expression of an activated form of Notchl in developing T cells of the mouse leads to both an increase in CD8 lineage T cells and a decrease in CD4 lineage T cells. Expression of activated Notch permits the development of mature CD8 lineage thymocytes even in the absence of class I major histocompatability complex (MHC) proteins, ligands that are normally required for the development of these cells. However, activated Notch is not sufficient to promote CD8 when both class I and class II MHC are absent. These results implicate Notch as a participant in the CD4 versus CD8 lineage decision. Robey, E. et al . Cell 87 : 483-492 (1996) .

Mutations in a gene region called CADASIL (for cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy) on chromosome 19 are associated with a type of stroke and dementia whose key features include recurrent subcortical ischaemic events and vascular dementia. Notch3 has been mapped to this region, and mutations in CADASIL patients indicate that Notch3 could be the defective protein in CADASIL patients, Joutel, A. et al . Na ture 383 : 101 - 110 (1996) .

Notch Ligands

There is also a conserved family of ligands for the Notch receptor family. Multiple ligands are able to activate the same receptor. For example, delta and serrate each act as ligands for Drosophila Notch. These ligands all contain EGF repeats (from 1-14), a DSL domain (delta, serrate, lag-2) and a transmembrane domain. Therefore, receptor and ligand are homologous to one another. In addition, receptor and ligand are often coexpressed and are associated with each other in vesicles .

Zneul Structure

Zneul is similar to Notch and its ligands in having two EGF repeats. However, it has a small number of EGF repeats and lacks a membrane spanning domain, linl2/Notch domains and ankyrin repeats. Based on structure/function experiments of Notch, one would predict that Zneul would antagonize Notch function. If the EGF repeats in zneul could bind receptor, it could inhibit ligand binding on neighboring cells. Furthermore, Zneul may have its own target receptor for which it would be an agonist .

Zneul Tissue Distribution/Multiple mRNA sizes

Zneul is widely expressed in adult human tissues. Zneul is most highly expressed in heart, placenta, spleen, testis, thyroid, spinal cord and lymph node. Dot blots indicate that Zneul is also expressed in a variety of fetal tissues. There are at least three mRNA sizes :

1.3 kb mRNA only in brain and testis 1.7 kb only in lymph node

1.3 + 1.7 in multiple tissues

2.4 kb only in placenta

Since the sequence of Zneul is from the 1.3 kb mRNA in brain, it is difficult to predict what types of molecules the larger transcripts encode. It is possible that larger forms could encode soluble Zneul proteins with more EGF repeats and other domains observed in Notch or Notch ligands. Alternatively, the extra sequences could encode transmembrane and intracellular domains.

Possible relationship to Notch function

It is difficult to predict whether Zneul will act as a Notch ligand or to antagonize the activity of other Notch ligands by competing for receptor binding. Zneul may alter the binary decisions in differentiation of stem cells into specific lineages or may alter the cell fate decisions of adjacent cells.

Alternatively, Zneul may have nothing to do with Notch. Many proteins have EGF repeats. Zneul may act as a growth factor for a different class of receptor.

Other Possible Roles

• role in breast cancer (EGF-receptor is overexpressed in many breast cancers) • role in glioblastomas, pituitary adenomas .

Mapping Data

Zneul maps to human chromosome 9q34.3, in the same chromosomal band as Notchl. It is of interest that Notch4 and HSMHC3W5A are also linked at the MHC III locus, i.e., duplication of an authentic Notch receptor and a 2 EGF-repeat novel protein.

Therapeutic utility

Zneul and its antagonists can be used as therapeutic reagents for the following.

1. Alzheimer's disease

The Sell2 gene was identified as a suppresser of a linl2 gain-of-function mutant. Sell2 is a homolog of a positional cloned human early-onset familial Alzheimer's disease gene. Therefore, Zneul could affect a pathway affecting this disease and it is expressed in brain, albeit at lower levels than most other tissues.

2. Cancer

There are a number of chromosomal rearrangements associated with breakpoints at 9q34 including Non-

Hodgkin's lymphoma and acute myeloid leukemia. A probe for Zneul which does not properly hybridize to chromosome 9q34 would indicate an abnormality of chromosome 9 and would indicate a possible predilection of the individual for developing cancer. Given the possible association with Notch 4, an endothelial-specific gene, Zneul could be involved in promoting or inhibiting endothelial cell tumors such as hemangiopericytomas? Another possibility is in angiogenesis since blocking a tumor's blood supply would be an effective cancer treatment.

Given the tissues where Zneul is highly expressed, the most prevalent forms of cancer would be in the testis and lymph nodes.

3. Hematopoiesis

Moore et al (PNAS 94:4011-4016, 1997) implicated delta-like (a mammalian Notch ligand) in promoting both high-proliferative potential progenitors and in stem cell repopulation. Since Zneul is highly expressed in lymph node and spleen, it could either be involved in inhibiting differentiation to promote stem cell self-renewal or in determination of progenitor populations. Possible use in repopulating blood cells after chemotherapy treatment or in vi tro expansion of stem cells.

Heart

Stimulation of myofibroblast proliferation or migration in the repair process after myocardial infarction. Recently, a frizzled homolog has been implicated in this process. There is evidence for interactions between the frizzled and Notch pathways in Drosophila.

5. Placenta

Stimulation or inhibition of various growth factor made in placenta. 6. Testis

Role in fertility or contraception

7. Spinal cord

Zneul may play a role in Nerve regeneration since Notch plays a role in neurogenesis in both flies and mammalian cells.

The present invention also provides reagents with significant therapeutic value. The Zneul polypeptide (naturally occurring or recombinant), fragments thereof, antibodies and anti-idiotypic antibodies thereto, along with compounds identified as having binding affinity to the Zneul polypeptide, should be useful in the treatment of conditions associated with abnormal physiology or development, including abnormal proliferation, e.g., cancerous conditions, or degenerative conditions. For example, a disease or disorder associated with abnormal expression or abnormal signaling by a Zneul polypeptide should be a likely target for an agonist or antagonist of the Zneul polypeptide.

Antibodies to the Zneul polypeptide can be purified and then administered to a patient. These reagents can be combined for therapeutic use with additional active or inert ingredients, e.g., in pharmaceutically acceptable carriers or diluents along with physiologically innocuous stabilizers and excipients. These combinations can be sterile filtered and placed into dosage forms as by lyophilization in dosage vials or storage in stabilized aqueous preparations. This invention also contemplates use of antibodies, binding fragments thereof or single-chain antibodies of the antibodies including forms which are not complement binding.

The quantities of reagents necessary for effective therapy will depend upon many different factors, including means of administration, target site, physiological state of the patient, and other medications administered. Thus, treatment dosages should be titrated to optimize safety and efficacy. Typically, dosages used in vi tro may provide useful guidance in the amounts useful for in vivo administration of these reagents . Animal testing of effective doses for treatment of particular disorders will provide further predictive indication of human dosage. Methods for administration include oral, intravenous, peritoneal, intramuscular, or transdermal administration. Pharmaceutically acceptable carriers will include water, saline, buffers to name just a few. Dosage ranges would ordinarily be expected from lμg to lOOOμg per kilogram of body weight per day. However, the doses by be higher or lower as can be determined by a medical doctor with ordinary skill in the art . For a complete discussion of drug formulations and dosage ranges see Remington ' s Pharmaceutical Sciences , 11 Ed., (Mack Publishing Co., Easton, Penn., 1990), and Goodman and Gilman ' s : The Pharmacological Bases of Therapeutics , 9 Ed. (Pergamon Press 1996) .

Nucleic Acid-based Therapeutic Treatment

If a mammal has a mutated or lacks a Zneul gene, the Zneul gene can be introduced into the cells of the mammal. In one embodiment, a gene encoding a Zneul polypeptide is introduced in vivo in a viral vector. Such vectors include an attenuated or defective DNA virus, such as but not limited to herpes simplex virus (HSV) , papillomavirus, Epstein Barr virus (EBV), adenovirus, adeno-associated virus (AAV), and the like. Defective viruses , which entirely or almost entirely lack viral genes, are preferred. A defective virus is not infective after introduction into a cell. Use of defective viral vectors allows for administration to cells in a specific, localized area, without concern that the vector can infect other cells. Examples of particular vectors include, but are not limited to, a defective herpes virus 1 (HSV1) vector [Kaplitt et al . , Mol ec . Cell . Neurosci . , 2 :320-330 (1991)], an attenuated adenovirus vector, such as the vector described by Stratford-Perricaudet et al . , J. Clin . Invest . , 90 :626-630 (1992), and a defective adeno- associated virus vector [Samulski et al . , J. Virol . , 61:3096-3101 (1987); Samulski et al . J. Virol . , 63:3822- 3828 (1989) ] .

In another embodiment, the gene can be introduced in a retroviral vector, e.g., as described in Anderson et al . , U.S. Patent No. 5,399,346; Mann et al . , Cell , 33:153 (1983); Temin et al . , U.S. Patent No. 4,650,764; Temin et al . , U.S. Patent No. 4,980,289; Markowitz et al . , J^". Virol . , 62:1120 (1988); Temin et al . , U.S. Patent No. 5,124,263; International Patent Publication No. WO 95/07358, published March 16, 1995 by Dougherty et al . ; and Blood, 82:845 (1993).

Alternatively, the vector can be introduced by lipofection in vivo using liposomes. Synthetic cationic lipids can be used to prepare liposomes for in vivo transfection of a gene encoding a marker [Feigner et al . , Proc . Na tl . Acad . Sci . USA, 84:7413-7417 (1987); see Mackey et al . , Proc . Natl . Acad . Sci . USA, 85:8027-8031 (1988) ] . The use of lipofection to introduce exogenous genes into specific organs in vivo has certain practical advantages. Molecular targeting of liposomes to specific cells represents one area of benefit. It is clear that directing transfection to particular cells represents one area of benefit. It is clear that directing transfection to particular cell types would be particularly advantageous in a tissue with cellular heterogeneity, such as the pancreas, liver, kidney, and brain. Lipids may be chemically coupled to other molecules for the purpose of targeting. Targeted peptides, e.g., hormones or neurotransmitters, and proteins such as antibodies, or non-peptide molecules could be coupled to liposomes chemically.

It is possible to remove the cells from the body and introduce the vector as a naked DNA plasmid and then re-implant the transformed cells into the body. Naked DNA vector for gene therapy can be introduced into the desired host cells by methods known in the art, e.g., transfection, electroporation, microinjection, transduction, cell fusion, DEAE dextran, calcium phosphate precipitation, use of a gene gun or use of a DNA vector transporter [see, e.g., Wu et al . , J. Biol . Chem . ,

267:963-967 (1992); Wu et al . , J. Biol . Chem . , 263:14621- 14624 (1988) ] .

ANTIBODIES

ZNEU1 polypeptides can also be used to prepare antibodies that specifically bind to Zneul epitopes, peptides or polypeptides. The Zneul polypeptide or a fragment thereof serves as an antigen (immunogen) to inoculate an animal and elicit an immune response.

Suitable antigens would be the Zneul polypeptide encoded by SEQ ID NO: 2 or 3 or at least a contiguous 9 amino acid fragment thereof. Antibodies generated from this immune response can be isolated and purified as described herein. Methods for preparing and isolating polyclonal and monoclonal antibodies are well known in the art. See, for example, Current Protocols in Immunology, Cooligan, et al . (eds.), National Institutes of Health, (John Wiley and Sons, Inc., 1995); Sambrook et al . , Molecular Cloning: A Labora tory Manual , Second Edi tion (Cold Spring Harbor, NY, 1989); and Hurrell, J. G. R. , Ed., Monoclonal Hybridoma Antibodies : Techniques and Applica tions (CRC Press, Inc., Boca Raton, FL, 1982) .

As would be evident to one of ordinary skill in the art, polyclonal antibodies can be generated from inoculating a variety of warm-blooded animals such as horses, cows, goats, sheep, dogs, chickens, rabbits, mice, and rats with a Zneul polypeptide or a fragment thereof. The immunogenicity of a Zneul polypeptide may be increased through the use of an adjuvant, such as alum (aluminum hydroxide) or Freund's complete or incomplete adjuvant. Polypeptides useful for immunization also include fusion polypeptides, such as fusions of Zneul or a portion thereof with an immunoglobulin polypeptide or with maltose binding protein. The polypeptide immunogen may be a full- length molecule or a portion thereof. If the polypeptide portion is "hapten-like" , such portion may be advantageously joined or linked to a macromolecular carrier (such as keyhole limpet hemocyanin (KLH) , bovine serum albumin (BSA) or tetanus toxoid) for immunization.

As used herein, the term "antibodies" includes polyclonal antibodies, affinity-purified polyclonal antibodies, monoclonal antibodies, and antigen-binding fragments, such as F(ab')2 ^anc Fab proteolytic fragments. Genetically engineered intact antibodies or fragments, such as chimeric antibodies, Fv fragments, single chain antibodies and the like, as well as synthetic antigen- binding peptides and polypeptides, are also included. Non-human antibodies may be humanized by grafting non- human CDRs onto human framework and constant regions, or by incorporating the entire non-human variable domains (optionally "cloaking" them with a human-like surface by replacement of exposed residues, wherein the result is a "veneered" antibody) . In some instances, humanized antibodies may retain non-human residues within the human variable region framework domains to enhance proper binding characteristics. Through humanizing antibodies, biological half-life may be increased, and the potential for adverse immune reactions upon administration to humans is reduced.

Alternative techniques for generating or selecting antibodies useful herein include in vi tro exposure of lymphocytes to Zneul protein or peptide, and selection of antibody display libraries in phage or similar vectors (for instance, through use of immobilized or labeled Zneul protein or peptide) . Genes encoding polypeptides having potential Zneul polypeptide binding domains can be obtained by screening random peptide libraries displayed on phage (phage display) or on bacteria, such as E. coli . Nucleotide sequences encoding the polypeptides can be obtained in a number of ways, such as through random mutagenesis and random polynucleotide synthesis. These random peptide display libraries can be used to screen for peptides which interact with a known target which can be a protein or polypeptide, such as a ligand or receptor, a biological or synthetic macromolecule, or organic or inorganic substances.

Techniques for creating and screening such random peptide display libraries are known in the art (Ladner et al . , US

Patent NO. 5,223,409; Ladner et al . , US Patent NO.

4,946,778; Ladner et al . , US Patent NO. 5,403,484 and Ladner et al . , US Patent NO. 5,571,698) and random peptide display libraries and kits for screening such libraries are available commercially, for instance from Clontech (Palo Alto, CA) , Invitrogen Inc. (San Diego, CA) , New England Biolabs, Inc. (Beverly, MA) and Pharmacia LKB Biotechnology Inc. (Piscataway, NJ) . Random peptide display libraries can be screened using the Zneul sequences disclosed herein to identify proteins which bind to Zneul. These "binding proteins" which interact with Zneul polypeptides can be used for tagging cells; for isolating homolog polypeptides by affinity purification; they can be directly or indirectly conjugated to drugs, toxins, radionuclides and the like. These binding proteins can also be used in analytical methods such as for screening expression libraries and neutralizing activity. The binding proteins can also be used for diagnostic assays for determining circulating levels of polypeptides; for detecting or quantitating soluble polypeptides as marker of underlying pathology or disease. These binding proteins can also act as Zneul "antagonists" to block Zneul binding and signal transduction in vi tro and in vivo . These anti-Zneul binding proteins would be useful for down regulating the effect of Zneul.

Antibodies are determined to be specifically binding if: 1) they exhibit a threshold level of binding activity, and/or 2) they do not significantly cross-react with related polypeptide molecules. First, antibodies herein specifically bind if they bind to a Zneul polypeptide, peptide or epitope with a binding affinity

(K_a) of 10 M or greater, preferably 10 M or greater,

8 —1 more preferably 10 M or greater, and most preferably 10 9 M-1 or greater. The binding affinity of an antibody - can be readily determined by one of ordinary skill in the art, for example, by Scatchard analysis.

Second, antibodies are determined to specifically bind if they do not significantly cross-react with related polypeptides. Antibodies do not significantly cross-react with related polypeptide molecules, for example, if they detect Zneul but not known related polypeptides using a standard Western blot analysis (Ausubel et al . , ibid. ) . Examples of known related polypeptides are orthologs, proteins from the same species that are members of a protein family (e.g. IL-16) , Zneul polypeptides, and non-human Zneul. Moreover, antibodies may be "screened against" known related polypeptides to isolate a population that specifically binds to the inventive polypeptides. For example, antibodies raised to Zneul are adsorbed to related polypeptides adhered to insoluble matrix; antibodies specific to Zneul will flow through the matrix under the proper buffer conditions. Such screening allows isolation of polyclonal and monoclonal antibodies non-crossreactive to closely related polypeptides, Antibodies : A Labora tory Manual , Harlow and Lane (eds.) (Cold Spring Harbor Laboratory Press, 1988); Current Protocols in Immunology, Cooligan, et al . (eds.), National Institutes of Health (John Wiley and Sons, Inc., 1995) . Screening and isolation of specific antibodies is well known in the art. See, Fundamental Immunology, Paul (eds.) (Raven Press, 1993); Getzoff et al . , Adv. in Immunol . 43 : 1-98 (1988); Monoclonal An tibodies : Principles and Practice, Goding, J.W. (eds.), (Academic Press Ltd., 1996); Benjamin et al . , Ann . Rev. Immunol . 2 : 67-101 (1984). A variety of assays known to those skilled in the art can be utilized to detect antibodies which specifically bind to Zneul proteins or peptides. Exemplary assays are described in detail in Antibodies : A Labora tory Manual , Harlow and Lane (Eds.) (Cold Spring Harbor Laboratory Press, 1988) . Representative examples of such assays include: concurrent immunoelectrophoresis, radioimmunoassay, radioimmuno-precipitation, enzyme-linked immunosorbent assay (ELISA) , dot blot or Western blot assay, inhibition or competition assay, and sandwich assay. In addition, antibodies can be screened for binding to wild-type versus mutant Zneul protein or polypeptide .

Antibodies to Zneul may be used for tagging cells that express Zneul; for isolating Zneul by affinity purification; for diagnostic assays for determining circulating levels of Zneul polypeptides; for detecting or quantitating soluble Zneul as marker of underlying pathology or disease; in analytical methods employing

FACS; for screening expression libraries; for generating anti-idiotypic antibodies; and as neutralizing antibodies or as antagonists to block Zneul in vi tro and in vivo . Suitable direct tags or labels include radionuclides, enzymes, substrates, cofactors, inhibitors, fluorescent markers, chemiluminescent markers, magnetic particles and the like; indirect tags or labels may feature use of biotin-avidin or other complement/anti-complement pairs as intermediates. Antibodies herein may also be directly or indirectly conjugated to drugs, toxins, radionuclides and the like, and these conjugates used for in vivo diagnostic or therapeutic applications. Moreover, antibodies to Zneul or fragments thereof may be used in vi tro to detect denatured Zneul or fragments thereof in assays, for example, Western Blots or other assays known in the art. An additional embodiment of the present invention relates to a peptide or polypeptide which has the amino acid sequence of an epitope-bearing portion of a Zneul polypeptide having an amino acid sequence described above. Peptides or polypeptides having the amino acid sequence of an epitope-bearing portion of a Zneul polypeptide of the present invention include portions of such polypeptides with at least nine, preferably at least 15 and more preferably at least 30 to 50 amino acids, although epitope-bearing polypeptides of any length up to and including the entire amino acid sequence of a polypeptide of the present invention described above are also included in the present invention. Examples of said polypeptides are defined by the amino acid sequences of SEQ ID NOs: 20-23. Also claimed are any of these polypeptides that are fused to another polypeptide or carrier molecule.

The invention is further illustrated by the following non-limiting examples.

Example 1. Cloning of Zneul

Zneul was identified from expressed sequence tag (EST) SEQ ID NO: 4. The cDNA clone containing the EST was discovered in a brain cDNA library which contained the EST. The cDNA was isolated from E . coli transfected with the plasmid and then streaked out on an LB 100 μg/ml ampicillin and 100 μg/ml methicillin plate. The cDNA insert was sequenced. The insert was determined to be 1514 base pairs long with a 274 amino acid open reading frame and a putative 19 amino acid signal peptide. Example 2

Northern Blot Analysis

Human multiple tissue blots 1,2,3 (Clontech) were probed to determine the tissue distribution of Zneul. A Hindl ll /NotI fragment containing the entire Zneul coding region was generated from the isolated cDNA clone and used for the probe. A plasmid prep of the clone was prepared from a 5 ml LB 100 μg/ml ampicillin overnight culture at 37° using the QIAprep Spin Miniprep Kit (Qiagen) . 20 μl out of 100 μl were digested with 3 μl of NEB Buffer 3, 10 units of HindiII (Gibco BRL) and 10 units Notl (New England Biolabs) in a 30 μl reaction at 37°C for 2 hours. The digest was electrophoresed on a 0.8% TBE agarose gel and the fragment was cut out. The DNA was extracted from the gel slab with a QIAquick Gel Extraction Kit (Qiagen) . 25 ng of this DNA was labeled with P³² using the Multiprime DNA Labeling System (Amersham) and unincorporated radioactivity was removed with a NucTrap Probe Purification Column (Stratagene) . Multiple tissue northerns and a human RNA master blot were prehybridized 3 hours with 10 ml ExpressHyb Solution and added to blots. Hybridization was carried out overnight at 42°C with a 10 ml solution of probe containing a concentration of 2 x 10^δ/ml of probe to which 1 mg of salmon sperm DNA was added which had been boiled for 5 minutes and then iced 1 minute and added to 10 ml of ExpressHyb Solution (Clontech) . Initial wash conditions were as follows: 2X SSC, 0.05% SDS RT for 40 minutes with several changes of solution then 0.1X SSC, 0.1% SDS at 65°C for 40 minutes, 1 solution change. Blots were than exposed to film a -80°C. There was cross hybridization/background so blots were further washed at 72°C then 65°C with 0.1% X SSC, 0.1% SDS for 1 hour each. The results showed that Zneul is widely expressed in adult tissues. Zneul is highly expressed in heart, placenta, spleen, testis, thyroid, spinal cord and lymph node. There are at least three mRNA sizes:

1.3 kb mRNA only in brain and testis;

1.4 kb only in lymph node;

1.5 + 1.7 kb in multiple tissues; and

2.4 kb only in placenta.

Example 3

Chromosomal Assignment and Placement of Zneul.

Zneul was mapped to chromosome 9 using the commercially available "GeneBridge 4 Radiation Hybrid Panel" (Research Genetics, Inc., Huntsville, AL) . The GeneBridge 4 Radiation Hybrid Panel contains PCRable DNAs from each of 93 radiation hybrid clones, plus two control DNAs (the HFL donor and the A23 recipient) . A publicly available WWW server (http://www-genome.wi.mit.edu/cgi- bin/contig/rhmapper .pi) allows mapping relative to the Whitehead Institute/MIT Center for Genome Research's radiation hybrid map of the human genome (the "WICGR" radiation hybrid map) which was constructed with the GeneBridge 4 Radiation Hybrid Panel.

For the mapping of Zneul with the "GeneBridge 4 RH Panel", 20 μl reactions were set up in a PCRable 96-well microtiter plate (Stratagene, La Jolla, CA) and used in a "RoboCycler Gradient 96" thermal cycler (Stratagene) . Each of the 95 PCR reactions consisted of 2 μl 10X KlenTaq PCR^' reaction buffer (CLONTECH Laboratories, Inc., Palo Alto, CA) , 1.6 μl dNTPs mix (2.5 mM each, PERKIN-ELMER, Foster City, CA) , 1 μl sense primer, SEQ ID NO: 6, 1 μl antisense primer, SEQ ID NO: 7, 2 μl "RediLoad" (Research Genetics, Inc., Huntsville, AL) , 0.4 μl 50X Advantage KlenTaq Polymerase Mix (Clontech Laboratories, Inc.), 25 ng of DNA from an individual hybrid clone or control and x μl ddH20 for a total volume of 20 μl . The reactions were overlaid with an equal amount of mineral oil and sealed. The PCR cycler conditions were as follows: an initial 1 cycle 5 minute denaturation at 95°C, 35 cycles of a 1 minute denaturation at 95°C, 1 minute annealing at 70°C and 1.5 minute extension at 72°C, followed by a final 1 cycle extension of 7 minutes at 72°C. The reactions were separated by electrophoresis on a 2% agarose gel (Life Technologies, Gaithersburg, MD) .

The results showed that Zneul maps 529.80 cR_3000 from the top of the human chromosome 9 linkage group on the WICGR radiation hybrid map, 7.90 cR_3000 distal of framework marker D9S158. This positions Zneul in the

9q34.3 region on the integrated LDB chromosome 9 map (The Genetic Location Database, University of Southhampton, WWW server: http: //cedar . genetics . soton.ac.uk/public html/) .

SEQUENCE LISTING

(1) GENERAL INFORMATION

(1) APPLICANT: ZymoGenetics, Inc.

1201 Eastlake Ave East

Seattle

WA

USA

98102

(ii) TITLE OF THE INVENTION: MAMMALIAN NEURO-GROWTH FACTOR LIKE PROTEIN

(ill) NUMBER OF SEQUENCES: 24

(iv) CORRESPONDENCE ADDRESS:

(A) ADDRESSEE: Zymogenetics

(B) STREET: 1201 Eastlake Ave East

(C) CITY: Seattle

(D) STATE: WA

(E) COUNTRY: USA

(F) ZIP: 98102

(v) COMPUTER READABLE FORM:

(A) MEDIUM TYPE: Diskette

(B) COMPUTER: IBM Compatible

(C) OPERATING SYSTEM: DOS

(D) SOFTWARE: FastSEQ for Windows Version 2.0

(vi) CURRENT APPLICATION DATA:

(A) APPLICATION NUMBER:

(B) FILING DATE:

(C) CLASSIFICATION:

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUMBER:

(B) FILING DATE:

(viii) ATTORNEY/AGENT INFORMATION:

(A) NAME: Lunn, Paul G

(B) REGISTRATION NUMBER: 32,743 (C) REFERENCE/DOCKET NUMBER: 97-28PC

(ix) TELECOMMUNICATION INFORMATION:

(A) TELEPHONE: 206-442-6627

(B) TELEFAX: 206-442-6678

(C) TELEX:

(2) INFORMATION FOR SEQ ID NO:l:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1297 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA (ix) FEATURE:

(A) NAME/KEY: Coding Sequence

(B) LOCATION: 69...887 (D) OTHER INFORMATION:

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:l:

AAGCTTGGCA CGAGGTGGCA CGAGGCCTCG TGCCAAGCTT GGCACGAGGC CGCCTGGAGG 60 CACAGGCC ATG AGG GGC TCT CAG GAG GTG CTG CTG ATG TGG CTT CTG GTG 110 Met Arg Gly Ser Gin Glu Val Leu Leu Met Trp Leu Leu Val 1 5 10

TTG GCA GTG GGC GGC ACA GAG CAC GCC TAC CGG CCC GGC CGT AGG GTG 158 Leu Ala Val Gly Gly Thr Glu His Ala Tyr Arg Pro Gly Arg Arg Val 15 20 25 30

TGT GCT GTC CGG GCT CAC GGG GAT CCT GTC TCC GAG TCG TTC GTG CAG 206 Cys Ala Val Arg Ala His Gly Asp Pro Val Ser Glu Ser Phe Val Gin 35 40 45

CGT GTG TAC CAG CCC TTC CTC ACC ACC TGC GAC GGG CAC CGG GCC TGC 254 Arg Val Tyr Gin Pro Phe Leu Thr Thr Cys Asp Gly His Arg Ala Cys 50 55 60

AGC ACC TAC CGA ACC ATC TAT AGG ACC GCC TAC CGC CGC AGC CCT GGG 302 Ser Thr Tyr Arg Thr He Tyr Arg Thr Ala Tyr Arg Arg Ser Pro Gly 65 70 75 CTG GCC CCT GCC AGG CCT CGC TAC GCG TGC TGC CCC GGC TGG AAG AGG 350 Leu Ala Pro Ala Arg Pro Arg Tyr Ala Cys Cys Pro Gly Trp Lys Arg 80 85 90

ACC AGC GGG CTT CCT GGG GCC TGT GGA GCA GCA ATA TGC CAG CCG CCA 398 Thr Ser Gly Leu Pro Gly Ala Cys Gly Ala Ala He Cys Gin Pro Pro 95 100 105 110

TGC CGG AAC GGA GGG AGC TGT GTC CAG CCT GGC CGC TGC CGC TGC CCT 446 Cys Arg Asn Gly Gly Ser Cys Val Gin Pro Gly Arg Cys Arg Cys Pro 115 120 125

GCA GGA TGG CGG GGT GAC ACT TGC CAG TCA GAT GTG GAT GAA TGC AGT 494 Ala Gly Trp Arg Gly Asp Thr Cys Gin Ser Asp Val Asp Glu Cys Ser 130 135 140

GCT AGG AGG GGC GGC TGT CCC CAG CGC TGC GTC AAC ACC GCC GGC AGT 542 Ala Arg Arg Gly Gly Cys Pro Gin Arg Cys Val Asn Thr Ala Gly Ser 145 150 155

TAC TGG TGC CAG TGT TGG GAG GGG CAC AGC CTG TCT GCA GAC GGT ACA 590 Tyr Trp Cys Gin Cys Trp Glu Gly His Ser Leu Ser Ala Asp Gly Thr 160 165 170

CTC TGT GTG CCC AAG GGA GGG CCC CCC AGG GTG GCC CCC AAC CCG ACA 638 Leu Cys Val Pro Lys Gly Gly Pro Pro Arg Val Ala Pro Asn Pro Thr 175 180 185 190

GGA GTG GAC AGT GCA ATG AAG GAA GAA GTG CAG AGG CTG CAG TCC AGG 686 Gly Val Asp Ser Ala Met Lys Glu Glu Val Gin Arg Leu Gin Ser Arg 195 200 205

GTG GAC CTG CTG GAG GAG AAG CTG CAG CTG GTG CTG GCC CCA CTG CAC 734

Val Asp Leu Leu Glu Glu Lys Leu Gin Leu Val Leu Ala Pro Leu His

210 215 220

AGC CTG GCC TCG CAG GCA CTG GAG CAT GGG CTC CCG GAC CCC GGC AGC 782

Ser Leu Ala Ser Gin Ala Leu Glu His Gly Leu Pro Asp Pro Gly Ser 225 230 235

CTC CTG GTG CAC TCC TTC CAG CAG CTC GGC CGC ATC GAC TCC CTG AGC 830

Leu Leu Val His Ser Phe Gin Gin Leu Gly Arg He Asp Ser Leu Ser

240 245 250 GAG CAG Aπ TCC TTC CTG GAG GAG CAG CTG GGG TCC TGC TCC TGC AAG 878 Glu Gin He Ser Phe Leu Glu Glu Gin Leu Gly Ser Cys Ser Cys Lys 255 260 265 270

AAA GAC TCG TGACTGCCCA GCGCCCCAGG CTGGACTGAG CCCCTCACGC CGCCCTGCA 936 Lys Asp Ser

GCCCCCATGC CCCTGCCCAA CATGCTGGGG GTCCAGAAGC CACCTCGGGG TGACTGAGCG 996

GAAGGCCAGG CAGGGCCTTC CTCCTCTTCC TCCTCCCCTT CCTCAGGAGG CTCCCCAGAC 1056

CCTGGCATGG GATGGGCTGG GATCTTCTCT GTGAATCCAC CCCTGGCTAC CCCCACCCTG 1116

GCTACCCCAA CGGCATCCCA AGGCCAGGTG GGCCCTCAGC TGAGGGAAGG TACGAGCTCC 1176

CTGCTGGAGC CTGGGACCCA TGGCACAGGC CAGGCAGCCC GGAGGCTGGG TGGGGCCTCA 1236

GTGGGGGCTG CTGCCTGACC CCCAGCACAA TAAAAATGAA ACGTGAAAAA AAAAAAAAAA 1296

A 1297

(2) INFORMATION FOR SEQ ID NO: 2:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 273 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein (v) FRAGMENT TYPE: internal

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:

Met Arg Gly Ser Gin Glu Val Leu Leu Met Trp Leu Leu Val Leu Ala 1 5 10 15

Val Gly Gly Thr Glu His Ala Tyr Arg Pro Gly Arg Arg Val Cys Ala

20 25 30

Val Arg Ala His Gly Asp Pro Val Ser Glu Ser Phe Val Gin Arg Val

35 40 45

Tyr Gin Pro Phe Leu Thr Thr Cys Asp Gly His Arg Ala Cys Ser Thr

50 55 60

Tyr Arg Thr He Tyr Arg Thr Ala Tyr Arg Arg Ser Pro Gly Leu Ala 65 70 75 80

Pro Ala Arg Pro Arg Tyr Ala Cys Cys Pro Gly Trp Lys Arg Thr Ser

85 90 95

Gly Leu Pro Gly Ala Cys Gly Ala Ala He Cys Gin Pro Pro Cys Arg

100 105 110

Asn Gly Gly Ser Cys Val Gin Pro Gly Arg Cys Arg Cys Pro Ala Gly 115 120 125 Trp Arg Gly Asp Thr Cys Gin Ser Asp Val Asp Glu Cys Ser Ala Arg

130 135 140

Arg Gly Gly Cys Pro Gin Arg Cys Val Asn Thr Ala Gly Ser Tyr Trp 145 150 155 160

Cys Gin Cys Trp Glu Gly His Ser Leu Ser Ala Asp Gly Thr Leu Cys

165 170 175

Val Pro Lys Gly Gly Pro Pro Arg Val Ala Pro Asn Pro Thr Gly Val

180 185 190

Asp Ser Ala Met Lys Glu Glu Val Gin Arg Leu Gin Ser Arg Val Asp

195 200 205

Leu Leu Glu Glu Lys Leu Gin Leu Val Leu Ala Pro Leu His Ser Leu

210 215 220

Ala Ser Gin Ala Leu Glu His Gly Leu Pro Asp Pro Gly Ser Leu Leu 225 230 235 240

Val His Ser Phe Gin Gin Leu Gly Arg He Asp Ser Leu Ser Glu Gin

245 250 255

He Ser Phe Leu Glu Glu Gin Leu Gly Ser Cys Ser Cys Lys Lys Asp

260 265 270

Ser

(2) INFORMATION FOR SEQ ID NO: 3:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 254 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:3:

Thr Glu His Ala Tyr Arg Pro Gly Arg Arg Val Cys Ala Val Arg Ala 1 5 10 15

His Gly Asp Pro Val Ser Glu Ser Phe Val Gin Arg Val Tyr Gin Pro

20 25 30

Phe Leu Thr Thr Cys Asp Gly His Arg Ala Cys Ser Thr Tyr Arg Thr

35 40 45

He Tyr Arg Thr Ala Tyr Arg Arg Ser Pro Gly Leu Ala Pro Ala Arg

50 55 60

Pro Arg Tyr Ala Cys Cys Pro Gly Trp Lys Arg Thr Ser Gly Leu Pro

65 70 75 80

Gly Ala Cys Gly Ala Ala He Cys Gin Pro Pro Cys Arg Asn Gly Gly 85 90 95 Ser Cys Val Gin Pro Gly Arg Cys Arg Cys Pro Ala Gly Trp Arg Gly

100 105 110

Asp Thr Cys Gin Ser Asp Val Asp Glu Cys Ser Ala Arg Arg Gly Gly

115 120 125

Cys Pro Gin Arg Cys Val Asn Thr Ala Gly Ser Tyr Trp Cys Gin Cys

130 135 140

Trp Glu Gly His Ser Leu Ser Ala Asp Gly Thr Leu Cys Val Pro Lys 145 150 155 160

Gly Gly Pro Pro Arg Val Ala Pro Asn Pro Thr Gly Val Asp Ser Ala

165 170 175

Met Lys Glu Glu Val Gin Arg Leu Gin Ser Arg Val Asp Leu Leu Glu

180 185 190

Glu Lys Leu Gin Leu Val Leu Ala Pro Leu His Ser Leu Ala Ser Gin

195 200 205

Ala Leu Glu His Gly Leu Pro Asp Pro Gly Ser Leu Leu Val His Ser

210 215 220

Phe Gin Gin Leu Gly Arg He Asp Ser Leu Ser Glu Gin He Ser Phe 225 230 235 240

Leu Glu Glu Gin Leu Gly Ser Cys Ser Cys Lys Lys Asp Ser 245 250

(2) INFORMATION FOR SEQ ID N0:4:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 284 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: Other

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:4:

GGCGGCGGCG CGTGCGCGCC CCGGATCCGG CGGCCACCCA GAGGAGAAGG CCACCCCGCC 60

TGGAGGCACA GGCCATGAGG GGCTCTCAGG AGGTGCTGCT GATGTGGCTT CTGGTGπGG 120

CAGTGGGCGG CACAGAGCAC GCCTACCGGC CCGGCCGTAG GGTGTGTGCT GTCCGGGCTC 180

ACGGGGACCC TGTCTCCGAG TCGTTCGTGC AGCGTGTGTA CCAGCCCTTC CTCACCACCT 240

GCGACGGGCA CCGGGCCTGC AGCACCTACC GAACCATCTA TAGG 284

(2) INFORMATION FOR SEQ ID NO: 5:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 40 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: Other

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:5:

TGCGGCGGTA GGCGGTCCTA TAGATGGπC GGTAGGTGCT 40

(2) INFORMATION FOR SEQ ID NO: 6:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 18 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: Other

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:6:

GCTGATGTGG CπCTGGT 18

(2) INFORMATION FOR SEQ ID NO: 7:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 18 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: Other (iv) ANTISENSE: YES

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:7:

GGTAGGCGTG CTCTGTGC 18

(2) INFORMATION FOR SEQ ID N0:8:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 708 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:8:

Thr His Arg Gly Leu His He Ser Ala Leu Ala Thr Tyr Arg Ala Arg

1 5 10 15

Gly Pro Arg Gly Leu Tyr Ala Arg Gly Ala Arg Gly Val Ala Leu Cys

20 25 30

Tyr Ser Ala Leu Ala Val Ala Leu Ala Arg Gly Ala Leu Ala His He

35 40 45

Ser Gly Leu Tyr Ala Ser Pro Pro Arg Val Ala Leu Ser Glu Arg Gly

50 55 60

Leu Ser Glu Arg Pro His Glu Val Ala Leu Gly Leu Asn Ala Arg Gly 65 70 75 80

Val Ala Leu Thr Tyr Arg Gly Leu Asn Pro Arg Pro His Glu Leu Glu

85 90 95

Thr His Arg Thr His Arg Cys Tyr Ser Ala Ser Pro Gly Leu Tyr His

100 105 110

He Ser Ala Arg Gly Ala Leu Ala Cys Tyr Ser Ser Glu Arg Thr His

115 120 125

Arg Thr Tyr Arg Ala Arg Gly Thr His Arg He Leu Glu Thr Tyr Arg

130 135 140

Ala Arg Gly Thr His Arg Ala Leu Ala Thr Tyr Arg Ala Arg Gly Ala 145 150 155 160

Arg Gly Ser Glu Arg Pro Arg Gly Leu Tyr Leu Glu Ala Leu Ala Pro

165 170 175

Arg Ala Leu Ala Ala Arg Gly Pro Arg Ala Arg Gly Thr Tyr Arg Ala

180 185 190

Leu Ala Cys Tyr Ser Cys Tyr Ser Pro Arg Gly Leu Tyr Thr Arg Pro

195 200 205

Leu Tyr Ser Ala Arg Gly Thr His Arg Ser Glu Arg Gly Leu Tyr Leu

210 215 220

Glu Pro Arg Gly Leu Tyr Ala Leu Ala Cys Tyr Ser Gly Leu Tyr Ala 225 230 235 240

Leu Ala Ala Leu Ala He Leu Glu Cys Tyr Ser Gly Leu Asn Pro Arg

245 250 255

Pro Arg Cys Tyr Ser Ala Arg Gly Ala Ser Asn Gly Leu Tyr Gly Leu

260 265 270

Tyr Ser Glu Arg Cys Tyr Ser Val Ala Leu Gly Leu Asn Pro Arg Gly

275 280 285

Leu Tyr Ala Arg Gly Cys Tyr Ser Ala Arg Gly Cys Tyr Ser Pro Arg

290 295 300

Ala Leu Ala Gly Leu Tyr Thr Arg Pro Ala Arg Gly Gly Leu Tyr Ala 305 310 315 320 Ser Pro Thr His Arg Cys Tyr Ser Gly Leu Asn Ser Glu Arg Ala Ser

325 330 335

Pro Val Ala Leu Ala Ser Pro Gly Leu Cys Tyr Ser Ser Glu Arg Ala

340 345 350

Leu Ala Ala Arg Gly Ala Arg Gly Gly Leu Tyr Gly Leu Tyr Cys Tyr

355 360 365

Ser Pro Arg Gly Leu Asn Ala Arg Gly Cys Tyr Ser Val Ala Leu Ala

370 375 380

Ser Asn Thr His Arg Ala Leu Ala Gly Leu Tyr Ser Glu Arg Thr Tyr 385 390 395 400

Arg Thr Arg Pro Cys Tyr Ser Gly Leu Asn Cys Tyr Ser Thr Arg Pro

405 410 415

Gly Leu Gly Leu Tyr His He Ser Ser Glu Arg Leu Glu Ser Glu Arg

420 425 430

Ala Leu Ala Ala Ser Pro Gly Leu Tyr Thr His Arg Leu Glu Cys Tyr

435 440 445

Ser Val Ala Leu Pro Arg Leu Tyr Ser Gly Leu Tyr Gly Leu Tyr Pro

450 455 460

Arg Pro Arg Ala Arg Gly Val Ala Leu Ala Leu Ala Pro Arg Ala Ser 465 470 475 480

Asn Pro Arg Thr His Arg Gly Leu Tyr Val Ala Leu Ala Ser Pro Ser

485 490 495

Glu Arg Ala Leu Ala Met Glu Thr Leu Tyr Ser Gly Leu Gly Leu Val

500 505 510

Ala Leu Gly Leu Asn Ala Arg Gly Leu Glu Gly Leu Asn Ser Glu Arg

515 520 525

Ala Arg Gly Val Ala Leu Ala Ser Pro Leu Glu Leu Glu Gly Leu Gly

530 535 540

Leu Leu Tyr Ser Leu Glu Gly Leu Asn Leu Glu Val Ala Leu Leu Glu 545 550 555 560

Ala Leu Ala Pro Arg Leu Glu His He Ser Ser Glu Arg Leu Glu Ala

565 570 575

Leu Ala Ser Glu Arg Gly Leu Asn Ala Leu Ala Leu Glu Gly Leu His

580 585 590

He Ser Gly Leu Tyr Leu Glu Pro Arg Ala Ser Pro Pro Arg Gly Leu

595 600 605

Tyr Ser Glu Arg Leu Glu Leu Glu Val Ala Leu His He Ser Ser Glu

610 615 620

Arg Pro His Glu Gly Leu Asn Gly Leu Asn Leu Glu Gly Leu Tyr Ala 625 630 635 640

Arg Gly He Leu Glu Ala Ser Pro Ser Glu Arg Leu Glu Ser Glu Arg

645 650 655

Gly Leu Gly Leu Asn He Leu Glu Ser Glu Arg Pro His Glu Leu Glu 660 665 670 Gly Leu Gly Leu Gly Leu Asn Leu Glu Gly Leu Tyr Ser Glu Arg Cys

675 680 685

Tyr Ser Ser Glu Arg Cys Tyr Ser Leu Tyr Ser Leu Tyr Ser Ala Ser

690 695 700

Pro Ser Glu Arg 705

(2) INFORMATION FOR SEQ ID N0:9:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 31 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:

Ala He Cys Gin Pro Pro Cys Arg Asn Gly Gly Ser Cys Val Gin Pro

1 5 10 15

Gly Arg Cys Arg Cys Pro Ala Gly Trp Arg Gly Asp Thr Cys Gin 20 25 30

(2) INFORMATION FOR SEQ ID NO: 10:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 42 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10:

Ser Asp Val Asp Glu Cys Ser Ala Arg Arg Gly Gly Cys Pro Gin Arg 1 5 10 15

Cys Val Asn Thr Ala Gly Ser Tyr Trp Cys Gin Cys Trp Glu Gly His

20 25 30

Ser Leu Ser Ala Asp Gly Thr Leu Cys Val 35 40

(2) INFORMATION FOR SEQ ID NO: 11:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 256 amino acids (B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11:

Pro Arg Leu Tyr Ser Gly Leu Tyr Gly Leu Tyr Pro Arg Pro Arg Ala 1 5 10 15

Arg Gly Val Ala Leu Ala Leu Ala Pro Arg Ala Ser Asn Pro Arg Thr

20 25 30

His Arg Gly Leu Tyr Val Ala Leu Ala Ser Pro Ser Glu Arg Ala Leu

35 40 45

Ala Met Glu Thr Leu Tyr Ser Gly Leu Gly Leu Val Ala Leu Gly Leu

50 55 60

Asn Ala Arg Gly Leu Glu Gly Leu Asn Ser Glu Arg Ala Arg Gly Val 65 70 75 80

Ala Leu Ala Ser Pro Leu Glu Leu Glu Gly Leu Gly Leu Leu Tyr Ser

85 90 95

Leu Glu Gly Leu Asn Leu Glu Val Ala Leu Leu Glu Ala Leu Ala Pro

100 105 110

Arg Leu Glu His He Ser Ser Glu Arg Leu Glu Ala Leu Ala Ser Glu

115 120 125

Arg Gly Leu Asn Ala Leu Ala Leu Glu Gly Leu His He Ser Gly Leu

130 135 140

Tyr Leu Glu Pro Arg Ala Ser Pro Pro Arg Gly Leu Tyr Ser Glu Arg 145 150 155 160

Leu Glu Leu Glu Val Ala Leu His He Ser Ser Glu Arg Pro His Glu

165 170 175

Gly Leu Asn Gly Leu Asn Leu Glu Gly Leu Tyr Ala Arg Gly He Leu

180 185 190

Glu Ala Ser Pro Ser Glu Arg Leu Glu Ser Glu Arg Gly Leu Gly Leu

195 200 205

Asn He Leu Glu Ser Glu Arg Pro His Glu Leu Glu Gly Leu Gly Leu

210 215 220

Gly Leu Asn Leu Glu Gly Leu Tyr Ser Glu Arg Cys Tyr Ser Ser Glu 225 230 235 240

Arg Cys Tyr Ser Leu Tyr Ser Leu Tyr Ser Ala Ser Pro Ser Glu Arg 245 250 255

(2) INFORMATION FOR SEQ ID NO: 12:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 331 amino acids (B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12:

Thr His Arg Gly Leu His He Ser Ala Leu Ala Thr Tyr Arg Ala Arg

1 5 10 15

Gly Pro Arg Gly Leu Tyr Ala Arg Gly Ala Arg Gly Val Ala Leu Cys

20 25 30

Tyr Ser Ala Leu Ala Val Ala Leu Ala Arg Gly Ala Leu Ala His He

35 40 45

Ser Gly Leu Tyr Ala Ser Pro Pro Arg Val Ala Leu Ser Glu Arg Gly

50 55 60

Leu Ser Glu Arg Pro His Glu Val Ala Leu Gly Leu Asn Ala Arg Gly 65 70 75 80

Val Ala Leu Thr Tyr Arg Gly Leu Asn Pro Arg Pro His Glu Leu Glu

85 90 95

Thr His Arg Thr His Arg Cys Tyr Ser Ala Ser Pro Gly Leu Tyr His

100 105 110

He Ser Ala Arg Gly Ala Leu Ala Cys Tyr Ser Ser Glu Arg Thr His

115 120 125

Arg Thr Tyr Arg Ala Arg Gly Thr His Arg He Leu Glu Thr Tyr Arg

130 135 140

Ala Arg Gly Thr His Arg Ala Leu Ala Thr Tyr Arg Ala Arg Gly Ala 145 150 155 160

Arg Gly Ser Glu Arg Pro Arg Gly Leu Tyr Leu Glu Ala Leu Ala Pro

165 170 175

Arg Ala Leu Ala Ala Arg Gly Pro Arg Ala Arg Gly Thr Tyr Arg Ala

180 185 190

Leu Ala Cys Tyr Ser Cys Tyr Ser Pro Arg Gly Leu Tyr Thr Arg Pro

195 200 205

Leu Tyr Ser Ala Arg Gly Thr His Arg Ser Glu Arg Gly Leu Tyr Leu

210 215 220

Glu Pro Arg Gly Leu Tyr Ala Leu Ala Cys Tyr Ser Gly Leu Tyr Ala 225 230 235 240

Leu Ala Ala Leu Ala He Leu Glu Cys Tyr Ser Gly Leu Asn Pro Arg

245 250 255

Pro Arg Cys Tyr Ser Ala Arg Gly Ala Ser Asn Gly Leu Tyr Gly Leu

260 265 270

Tyr Ser Glu Arg Cys Tyr Ser Val Ala Leu Gly Leu Asn Pro Arg Gly 275 280 285 Leu Tyr Ala Arg Gly Cys Tyr Ser Ala Arg Gly Cys Tyr Ser Pro Arg

290 295 300

Ala Leu Ala Gly Leu Tyr Thr Arg Pro Ala Arg Gly Gly Leu Tyr Ala 305 310 315 320

Ser Pro Thr His Arg Cys Tyr Ser Gly Leu Asn 325 330

(2) INFORMATION FOR SEQ ID NO 13

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 158 amino acids

(B) TYPE amino acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(π) MOLECULE TYPE protein

(xi) SEQUENCE DESCRIPTION SEQ ID NO 13

Thr Glu His Ala Tyr Arg Pro Gly Arg Arg Val Cys Ala Val Arg Ala 1 5 10 15

His Gly Asp Pro Val Ser Glu Ser Phe Val Gin Arg Val Tyr Gin Pro

20 25 30

Phe Leu Thr Thr Cys Asp Gly His Arg Ala Cys Ser Thr Tyr Arg Thr

35 40 45

He Tyr Arg Thr Ala Tyr Arg Arg Ser Pro Gly Leu Ala Pro Ala Arg

50 55 60

Pro Arg Tyr Ala Cys Cys Pro Gly Trp Lys Arg Thr Ser Gly Leu Pro 65 70 75 80

Gly Ala Cys Gly Ala Ala He Cys Gin Pro Pro Cys Arg Asn Gly Gly

85 90 95

Ser Cys Val Gin Pro Gly Arg Cys Arg Cys Pro Ala Gly Trp Arg Gly

100 105 110

Asp Thr Cys Gin Ser Asp Val Asp Glu Cys Ser Ala Arg Arg Gly Gly

115 120 125

Cys Pro Gin Arg Cys Val Asn Thr Ala Gly Ser Tyr Trp Cys Gin Cys

130 135 140

Trp Glu Gly His Ser Leu Ser Ala Asp Gly Thr Leu Cys Val 145 150 155

(2) INFORMATION FOR SEQ ID NO 14

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 73 amino acids

(B) TYPE amino acid (C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:

Ala He Cys Gin Pro Pro Cys Arg Asn Gly Gly Ser Cys Val Gin Pro

1 5 10 15

Gly Arg Cys Arg Cys Pro Ala Gly Trp Arg Gly Asp Thr Cys Gin Ser

20 25 30

Asp Val Asp Glu Cys Ser Ala Arg Arg Gly Gly Cys Pro Gin Arg Cys

35 40 45

Val Asn Thr Ala Gly Ser Tyr Trp Cys Gin Cys Trp Glu Gly His Ser

50 55 60

Leu Ser Ala Asp Gly Thr Leu Cys Val 65 70

(2) INFORMATION FOR SEQ ID NO: 15:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 169 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15:

Ala He Cys Gin Pro Pro Cys Arg Asn Gly Gly Ser Cys Val Gin Pro

1 5 10 15

Gly Arg Cys Arg Cys Pro Ala Gly Trp Arg Gly Asp Thr Cys Gin Ser

20 25 30

Asp Val Asp Glu Cys Ser Ala Arg Arg Gly Gly Cys Pro Gin Arg Cys

35 40 45

Val Asn Thr Ala Gly Ser Tyr Trp Cys Gin Cys Trp Glu Gly His Ser

50 55 60

Leu Ser Ala Asp Gly Thr Leu Cys Val Pro Lys Gly Gly Pro Pro Arg 65 70 75 80

Val Ala Pro Asn Pro Thr Gly Val Asp Ser Ala Met Lys Glu Glu Val

85 90 95

Gin Arg Leu Gin Ser Arg Val Asp Leu Leu Glu Glu Lys Leu Gin Leu 100 105 110 Val Leu Ala Pro Leu His Ser Leu Ala Ser Gin Ala Leu Glu His Gly

115 120 125

Leu Pro Asp Pro Gly Ser Leu Leu Val His Ser Phe Gin Gin Leu Gly

130 135 140

Arg He Asp Ser Leu Ser Glu Gin He Ser Phe Leu Glu Glu Gin Leu 145 150 155 160

Gly Ser Cys Ser Cys Lys Lys Asp Ser 165

(2) INFORMATION FOR SEQ ID NO: 16:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 181 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16:

Thr Glu His Ala Tyr Arg Pro Gly Arg Arg Val Cys Ala Val Arg Ala 1 5 10 15

His Gly Asp Pro Val Ser Glu Ser Phe Val Gin Arg Val Tyr Gin Pro

20 25 30

Phe Leu Thr Thr Cys Asp Gly His Arg Ala Cys Ser Thr Tyr Arg Thr

35 40 45

He Tyr Arg Thr Ala Tyr Arg Arg Ser Pro Gly Leu Ala Pro Ala Arg

50 55 60

Pro Arg Tyr Ala Cys Cys Pro Gly Trp Lys Arg Thr Ser Gly Leu Pro 65 70 75 80

Gly Ala Cys Gly Ala Pro Lys Gly Gly Pro Pro Arg Val Ala Pro Asn

85 90 95

Pro Thr Gly Val Asp Ser Ala Met Lys Glu Glu Val Gin Arg Leu Gin

100 105 110

Ser Arg Val Asp Leu Leu Glu Glu Lys Leu Gin Leu Val Leu Ala Pro

115 120 125

Leu His Ser Leu Ala Ser Gin Ala Leu Glu His Gly Leu Pro Asp Pro

130 135 140

Gly Ser Leu Leu Val His Ser Phe Gin Gin Leu Gly Arg He Asp Ser 145 150 155 160

Leu Ser Glu Gin He Ser Phe Leu Glu Glu Gin Leu Gly Ser Cys Ser

165 170 175

Cys Lys Lys Asp Ser 180 (2) INFORMATION FOR SEQ ID NO: 17:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 293 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:

Met Gly Ser Arg Ala Glu Leu Cys Thr Leu Leu Gly Gly Phe Ser Phe 1 5 10 15

Leu Leu Leu Leu He Pro Gly Glu Gly Ala Lys Gly Gly Ser Leu Arg

20 25 30

Glu Ser Gin Gly Val Cys Ser Lys Gin Thr Leu Val Val Pro Leu His

35 40 45

Tyr Asn Glu Ser Tyr Ser Gin Pro Val Tyr Lys Pro Tyr Leu Thr Leu

50 55 60

Cys Ala Gly Arg Arg He Cys Ser Thr Tyr Arg Thr Met Tyr Arg Val 65 70 75 80

Met Trp Arg Glu Val Arg Arg Glu Val Gin Gin Thr His Ala Val Cys

85 90 95

Cys Gin Gly Trp Lys Lys Arg His Pro Gly Ala Leu Thr Cys Glu Ala

100 105 110

He Cys Ala Lys Pro Cys Leu Asn Gly Gly Val Cys Val Arg Pro Asp

115 120 125

Gin Cys Glu Cys Ala Pro Gly Trp Gly Gly Lys His Cys His Val Asp

130 135 140

Val Asp Glu Cys Arg Thr Ser He Thr Leu Cys Ser His His Cys Phe 145 150 155 160

Asn Thr Ala Gly Ser Phe Thr Cys Gly Cys Pro His Asp Leu Val Leu

165 170 175

Gly Val Asp Gly Arg Thr Cys Met Glu Gly Ser Pro Glu Pro Pro Thr

180 185 190

Ser Ala Ser He Leu Ser Val Ala Val Arg Glu Ala Glu Lys Asp Glu

195 200 205

Arg Ala Leu Lys Gin Glu He His Glu Leu Arg Gly Arg Leu Glu Arg

210 215 220

Leu Glu Gin Trp Ala Gly Gin Ala Gly Ala Trp Val Arg Ala Val Leu 225 230 235 240

Pro Val Pro Pro Glu Glu Leu Gin Pro Glu Gin Val Ala Glu Leu Trp 245 250 255 Gly Arg Gly Asp Arg He Glu Ser Leu Ser Asp Gin Val Leu Leu Leu

260 265 270

Glu Glu Arg Leu Gly Ala Cys Ser Cys Glu Asp Asn Ser Leu Gly Leu

275 280 285

Gly Val Asn His Arg 290

(2) INFORMATION FOR SEQ ID NO: 18:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1339 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA (ix) FEATURE:

(A) NAME/KEY: Coding Sequence

(B) LOCATION: 261...1094 (D) OTHER INFORMATION:

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18:

GTAGGGCTCT GCCGGGACCT GGGTCTTCCC TCTCCTGGAG CTGCAGAGGC CAGAAGπCA 60

GTGGTGAGGG GTCCAAGGAG AGTCCGGGGA GACCAGGGAG GCTCTGTCCA TCCCCTGTCC 120

CTGTCCCTGT GGGAAGCCCC CGGCAGCAGC AAGACGCTGG CTGπCCACC TGCCCACAAG 180

AACAGCCACC ACCAGTACCC AGGGGATGAC AAGCGGCCGG ACCACAGGCC ACAAAAAGAA 240

GAAGGCTACC CCACTTACAG ATG CAG ACC ATG TGG GGC TCC GGA GAA CTG 290

Met Gin Thr Met Trp Gly Ser Gly Glu Leu 1 5 10

Cπ GTA GCA TGG Tπ CTA GTG TTG GCA GCA GAT GGT ACT ACT GAG CAT 338 Leu Val Ala Trp Phe Leu Val Leu Ala Ala Asp Gly Thr Thr Glu His 15 20 25

GTC TAC AGA CCC AGC CGT AGA GTG TGT ACT GTG GGG ATT TCC GGA GGT 386 Val Tyr Arg Pro Ser Arg Arg Val Cys Thr Val Gly He Ser Gly Gly 30 35 40

TCC ATC TCG GAG ACC πT GTG CAG CGT GTA TAC CAG CCT TAC CTC ACC 434 Ser He Ser Glu Thr Phe Val Gin Arg Val Tyr Gin Pro Tyr Leu Thr 45 50 55 ACT TGC GAC GGA CAC AGA GCC TGC AGC ACC TAC CGA ACC ATC TAC CGG 482 Thr Cys Asp Gly His Arg Ala Cys Ser Thr Tyr Arg Thr He Tyr Arg 60 65 70

ACT GCC TAT CGC CGT AGC CCT GGG GTG ACT CCC GCA AGG CCT CGC TAT 530 Thr Ala Tyr Arg Arg Ser Pro Gly Val Thr Pro Ala Arg Pro Arg Tyr 75 80 85 90

GCT TGC TGC CCT GGT TGG AAG AGG ACC AGT GGG CTC CCT GGG GCT TGT 578 Ala Cys Cys Pro Gly Trp Lys Arg Thr Ser Gly Leu Pro Gly Ala Cys 95 100 105

GGA GCA GCA ATA TGC CAG CCT CCA TGT GGG AAT GGA GGG AGT TGC ATC 626 Gly Ala Ala He Cys Gin Pro Pro Cys Gly Asn Gly Gly Ser Cys He 110 115 120

CGC CCA GGA CAC TGC CGC TGC CCT GTG GGA TGG CAG GGA GAT ACT TGC 674 Arg Pro Gly His Cys Arg Cys Pro Val Gly Trp Gin Gly Asp Thr Cys 125 130 135

CAG ACA GAT Gπ GAT GAA TGC AGT ACA GGA GAG GCC AGT TGT CCC CAG 722 Gin Thr Asp Val Asp Glu Cys Ser Thr Gly Glu Ala Ser Cys Pro Gin 140 145 150

CGC TGT GTC AAT ACT GTG GGA AGT TAC TGG TGC CAG GGA TGG GAG GGA 770 Arg Cys Val Asn Thr Val Gly Ser Tyr Trp Cys Gin Gly Trp Glu Gly 155 160 165 170

CAA AGC CCA TCT GCA GAT GGG ACG CGC TGC CTG TCT AAG GAG GGG CCC 818 Gin Ser Pro Ser Ala Asp Gly Thr Arg Cys Leu Ser Lys Glu Gly Pro 175 180 185

TCC CCG GTG GCC CCA AAC CCC ACA GCA GGA GTG GAC AGC ATG GCG AGA 866 Ser Pro Val Ala Pro Asn Pro Thr Ala Gly Val Asp Ser Met Ala Arg 190 195 200

GAG GAG GTG TAC AGG CTG CAG GCT CGG GTT GAT GTG CTA GAA CAG AAA 914 Glu Glu Val Tyr Arg Leu Gin Ala Arg Val Asp Val Leu Glu Gin Lys 205 210 215

CTG CAG TTG GTG CTG GCC CCA CTG CAC AGC CTG GCC TCT CGG TCC ACA 962 Leu Gin Leu Val Leu Ala Pro Leu His Ser Leu Ala Ser Arg Ser Thr 220 225 230 GAG CAT GGG CTA CAA GAT CCT GGC AGC CTG CTG GCC CAT TCC TTC CAG 1010 Glu His Gly Leu Gin Asp Pro Gly Ser Leu Leu Ala His Ser Phe Gin 235 240 245 250

CAG CTG GAC CGA Aπ GAT TCA CTG AGT GAG CAG GTG TCC πC πG GAG 1058 Gin Leu Asp Arg He Asp Ser Leu Ser Glu Gin Val Ser Phe Leu Glu 255 260 265

GAA CAT CTG GGG TCC TGC TCC TGC AAA AAA GAT CTG TGATAACCTC TCACCA 1110 Glu His Leu Gly Ser Cys Ser Cys Lys Lys Asp Leu 270 275

CCCAGGCTGG ATAGAGCAGT CATCCCTAGA TCCCTTGTAG CCAGAGTTCA GGCGCTGTCT 1170

GGTGGTGCCT ATGAGCAGAA GGCCCTGCCT CATTGTCCCT CπTCTTAGG AGGTTCCTAG 1230

GACTTGGGCA TGGGGAGTGG GGTCTTGTGT GACTCπCAG TGGGGCTCCC TGTCTAAGTG 1290

GTAAGGTGGG GAπGTCTCC ATCTTTGTCA TAATAAAGCT GAGACTTGA 1339

(2) INFORMATION FOR SEQ ID NO: 19:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 278 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein (v) FRAGMENT TYPE: internal

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:

Met Gin Thr Met Trp Gly Ser Gly Glu Leu Leu Val Ala Trp Phe Leu 1 5 10 15

Val Leu Ala Ala Asp Gly Thr Thr Glu His Val Tyr Arg Pro Ser Arg

20 25 30

Arg Val Cys Thr Val Gly He Ser Gly Gly Ser He Ser Glu Thr Phe

35 40 45

Val Gin Arg Val Tyr Gin Pro Tyr Leu Thr Thr Cys Asp Gly His Arg

50 55 60

Ala Cys Ser Thr Tyr Arg Thr He Tyr Arg Thr Ala Tyr Arg Arg Ser 65 70 75 80

Pro Gly Val Thr Pro Ala Arg Pro Arg Tyr Ala Cys Cys Pro Gly Trp

85 90 95

Lys Arg Thr Ser Gly Leu Pro Gly Ala Cys Gly Ala Ala He Cys Gin 100 105 110 Pro Pro Cys Gly Asn Gly Gly Ser Cys He Arg Pro Gly His Cys Arg

115 120 125

Cys Pro Val Gly Trp Gin Gly Asp Thr Cys Gin Thr Asp Val Asp Glu

130 135 140

Cys Ser Thr Gly Glu Ala Ser Cys Pro Gin Arg Cys Val Asn Thr Val 145 150 155 160

Gly Ser Tyr Trp Cys Gin Gly Trp Glu Gly Gin Ser Pro Ser Ala Asp

165 170 175

Gly Thr Arg Cys Leu Ser Lys Glu Gly Pro Ser Pro Val Ala Pro Asn

180 185 190

Pro Thr Ala Gly Val Asp Ser Met Ala Arg Glu Glu Val Tyr Arg Leu

195 200 205

Gin Ala Arg Val Asp Val Leu Glu Gin Lys Leu Gin Leu Val Leu Ala

210 215 220

Pro Leu His Ser Leu Ala Ser Arg Ser Thr Glu His Gly Leu Gin Asp 225 230 235 240

Pro Gly Ser Leu Leu Ala His Ser Phe Gin Gin Leu Asp Arg He Asp

245 250 255

Ser Leu Ser Glu Gin Val Ser Phe Leu Glu Glu His Leu Gly Ser Cys

260 265 270

Ser Cys Lys Lys Asp Leu 275

(2) INFORMATION FOR SEQ ID NO: 20:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 29 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:20:

Thr Cys Asp Gly His Arg Ala Cys Ser Thr Tyr Arg Thr He Tyr Arg

1 5 10 15

Thr Ala Tyr Arg Arg Ser Pro Gly Leu Ala Pro Ala Arg 20 25

(2) INFORMATION FOR SEQ ID NO: 21:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 32 amino acids

(B) TYPE: amino acid (C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21:

Gin Pro Gly Arg Cys Arg Cys Pro Ala Gly Trp Arg Gly Asp Thr Cys

1 5 10 15

Gin Ser Asp Val Asp Glu Cys Ser Ala Arg Arg Gly Gly Cys Pro Gin 20 25 30

(2) INFORMATION FOR SEQ ID N0:22:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 37 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:22:

Cys Val Pro Lys Gly Gly Pro Pro Arg Val Ala Pro Asn Pro Thr Gly 1 5 10 15

Val Asp Ser Ala Met Lys Glu Glu Val Gin Arg Leu Gin Ser Arg Val

20 25 30

Asp Leu Leu Glu Glu 35

(2) INFORMATION FOR SEQ ID NO: 23:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 29 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi ) SEQUENCE DESCRIPTION : SEQ ID NO: 23 :

Gi n Gi n Leu Gly Arg H e Asp Ser Leu Ser Gl u Gi n H e Ser Phe Leu

1 5 10 15

Gl u Gl u Gi n Leu Gly Ser Cys Ser Cys Lys Lys Asp Ser 20 25 (2) INFORMATION FOR SEQ ID NO: 24:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 255 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:24:

Thr Glu His Val Tyr Arg Pro Ser Arg Arg Val Cys Thr Val Gly He 1 5 10 15

Ser Gly Gly Ser He Ser Glu Thr Phe Val Gin Arg Val Tyr Gin Pro

20 25 30

Tyr Leu Thr Thr Cys Asp Gly His Arg Ala Cys Ser Thr Tyr Arg Thr

35 40 45

He Tyr Arg Thr Ala Tyr Arg Arg Ser Pro Gly Val Thr Pro Ala Arg

50 55 60

Pro Arg Tyr Ala Cys Cys Pro Gly Trp Lys Arg Thr Ser Gly Leu Pro 65 70 75 80

Gly Ala Cys Gly Ala Ala He Cys Gin Pro Pro Cys Gly Asn Gly Gly

85 90 95

Ser Cys He Arg Pro Gly His Cys Arg Cys Pro Val Gly Trp Gin Gly

100 105 110

Asp Thr Cys Gin Thr Asp Val Asp Glu Cys Ser Thr Gly Glu Ala Ser

115 120 125

Cys Pro Gin Arg Cys Val Asn Thr Val Gly Ser Tyr Trp Cys Gin Gly

130 135 140

Trp Glu Gly Gin Ser Pro Ser Ala Asp Gly Thr Arg Cys Leu Ser Lys 145 150 155 160

Glu Gly Pro Ser Pro Val Ala Pro Asn Pro Thr Ala Gly Val Asp Ser

165 170 175

Met Ala Arg Glu Glu Val Tyr Arg Leu Gin Ala Arg Val Asp Val Leu

180 185 190

Glu Gin Lys Leu Gin Leu Val Leu Ala Pro Leu His Ser Leu Ala Ser

195 200 205

Arg Ser Thr Glu His Gly Leu Gin Asp Pro Gly Ser Leu Leu Ala His

210 215 220

Ser Phe Gin Gin Leu Asp Arg He Asp Ser Leu Ser Glu Gin Val Ser 225 230 235 240

Phe Leu Glu Glu His Leu Gly Ser Cys Ser Cys Lys Lys Asp Leu 245 250 255

Claims

CLAIMS We claim:

1. An isolated polynucleotide which encodes a mammalian Zneul polypeptide wherein said polynucleotide encodes a polypeptide selected from the group SEQ ID NOs: 2- 3,8, 9, 11-16, and 19-24 or a polypeptide which is at least 90% identical to the polypeptides of said group and which retain the activity of said polypeptides.

2. An isolated polynucleotide which encodes a peptide or polypeptide having at least 15 amino acid residues comprised of an epitope-bearing portion of a polypeptide of SEQ ID NOs: 2-3,8, 9, 11-16, and 19-24 or a polypeptide which is at least 90% identical to said polypeptides.

3. The polynucleotide of claim 2 wherein the peptide or polypeptide is fused to a carrier polypeptide or other carrier molecule.

4. An expression vector comprising the following operably linked elements: a transcription promoter; a DNA segment which encodes a Zneul polypeptide or a peptide or polypeptide which contains an epitope-bearing region of a Zneul polypeptide; and a transcription terminator.

5. An expression vector comprising the following operably linked elements:

(a) a transcription promoter;

(b) a DNA segment encoding a chimeric polypeptide, wherein said chimeric polypeptide consists essentially of a first portion and a second portion joined by a peptide bond, said first portion being comprised of a mammalian polypeptide, said polypeptide being the amino acid sequences of SEQ ID NOs : 2-3,8, 9, 11-16, and 19-24 or a polypeptide which is at least 90% identical to said amino acid sequences and said second portion being a second polypeptide or protein, (c) a transcription terminator.

6. An isolated Zneul polypeptide selected from the group of amino acid sequences consisting of SEQ ID NOs: 2-3,8, 9, 11-16, and 19-24 or a polypeptide which is at least 90% identical to said polypeptides.

7. An isolated peptide or polypeptide having at least 15 amino acid residues comprised of an epitope-bearing portion of a polypeptide of SEQ ID NOs: 2-3,8, 9, 11-16, and 19-24 or is at least 90% identical to said epitope bearing portion.

8. The isolated peptide or polypeptide of claim 7 wherein the epitope-bearing portion is selected from the group of amino acid sequence consisting of SEQ ID NOs : 20-23 or a peptide or polypeptide which is at least 90% identical to said epitope bearing portion.

9. TΛn antibody, antibody fragment or single-chain antibody that specifically binds to a mammalian polypeptide, said polypeptide being defined by the amino acid sequences of SEQ ID NOs: 2-3,8, 9, 11-16, and 19-24.

10. The antibody, antibody fragment or single-chain antibody of claim 9 wherein said antibody, antibody fragment or single-chain antibody is humanized.

11. A method for producing an antibody which binds to a peptide or polypeptide defined by SEQ ID NOs: 2-3,8, 9, 11-16, and 19-24 or to a peptide or polypeptide which is at least 90% identical to said peptide or polypeptide comprising ^" inoculating an animal with said peptide or polypeptide or with a nucleic acid which encodes said peptide or polypeptide, wherein said animal produces antibodies to said peptide or polypeptide; and isolating said antibody.

12. An anti-idiotypic antibody, anti-idiotypic antibody fragment or anti-idiotypic single-chain antibody which binds to an antibody, an antibody fragment or single- chain antibody of peptide or polypeptide defined by SEQ ID NOs: 2-3,8, 9, 11-16, and 19-24 or to a peptide or polypeptide which is at least 90% identical to said peptide or polypeptide .

13. The antibodies of claims 9-12 wherein said antibodies are either polyclonal or monoclonal.