WO1998049286A3 - Directed evolution of enzymes and antibodies - Google Patents

Directed evolution of enzymes and antibodies Download PDF

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Publication number
WO1998049286A3
WO1998049286A3 PCT/US1998/008714 US9808714W WO9849286A3 WO 1998049286 A3 WO1998049286 A3 WO 1998049286A3 US 9808714 W US9808714 W US 9808714W WO 9849286 A3 WO9849286 A3 WO 9849286A3
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WO
WIPO (PCT)
Prior art keywords
methods
antibodies
enzymes
directed evolution
selecting
Prior art date
Application number
PCT/US1998/008714
Other languages
French (fr)
Other versions
WO1998049286A9 (en
WO1998049286A2 (en
Inventor
Brent Iverson
George Georgiou
Gang Chen
Mark J Olsen
Patrick S Daugherty
Original Assignee
Univ Texas
Brent Iverson
George Georgiou
Gang Chen
Mark J Olsen
Patrick S Daugherty
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas, Brent Iverson, George Georgiou, Gang Chen, Mark J Olsen, Patrick S Daugherty filed Critical Univ Texas
Priority to AU71710/98A priority Critical patent/AU7171098A/en
Publication of WO1998049286A2 publication Critical patent/WO1998049286A2/en
Publication of WO1998049286A3 publication Critical patent/WO1998049286A3/en
Publication of WO1998049286A9 publication Critical patent/WO1998049286A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to methods of selecting proteins, out of large libraries, having desirable characteristics. Exemplified are methods of expressing enzymes and antibodies on the surface of host cells and selecting for desired activities. These methods have the advantage of speed and ease of operation when compared with current methods. They also provide, without additional cloning, a source of significant quantities of the protein of interest.
PCT/US1998/008714 1997-05-01 1998-04-30 Directed evolution of enzymes and antibodies WO1998049286A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU71710/98A AU7171098A (en) 1997-05-01 1998-04-30 Directed evolution of enzymes and antibodies

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84706397A 1997-05-01 1997-05-01
US08/847,063 1997-05-01

Publications (3)

Publication Number Publication Date
WO1998049286A2 WO1998049286A2 (en) 1998-11-05
WO1998049286A3 true WO1998049286A3 (en) 1999-03-25
WO1998049286A9 WO1998049286A9 (en) 1999-04-29

Family

ID=25299655

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/008714 WO1998049286A2 (en) 1997-05-01 1998-04-30 Directed evolution of enzymes and antibodies

Country Status (2)

Country Link
AU (1) AU7171098A (en)
WO (1) WO1998049286A2 (en)

Families Citing this family (41)

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US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US7018793B1 (en) 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US6562594B1 (en) 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6699658B1 (en) 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
WO1999015897A1 (en) * 1997-09-19 1999-04-01 Chiron Corporation Subtractive protein screening for gene identification
KR100313135B1 (en) 1999-02-22 2001-11-05 윤덕용 Expression Vectors Comprising a Gene Encoding OmpC Derived from E.coli as Cell Surface Anchoring Motif
DE60040771D1 (en) 1999-03-04 2008-12-24 Asubio Pharma Co Ltd PROCESS FOR CONTROLLING THE CUTTING WITH OMPT PROTEASE
KR100312070B1 (en) * 1999-03-15 2001-11-03 박호군 Method of Selecting Enzyme Variants Including High Throughput Screening
WO2001032858A1 (en) * 1999-11-05 2001-05-10 Novozymes A/S A high throughput screening (hts) method
CN1451043A (en) 2000-06-29 2003-10-22 艾博特公司 Dual specificity antibodies and methods of making and using
US7083945B1 (en) * 2000-10-27 2006-08-01 The Board Of Regents Of The University Of Texas System Isolation of binding proteins with high affinity to ligands
US7094571B2 (en) * 2000-10-27 2006-08-22 The Board Of Regents Of The University Of Texas System Combinatorial protein library screening by periplasmic expression
US7611866B2 (en) 2002-07-15 2009-11-03 Board Of Regents, The University Of Texas System Selection of bacterial inner-membrane anchor polypeptides
DE10303974A1 (en) 2003-01-31 2004-08-05 Abbott Gmbh & Co. Kg Amyloid β (1-42) oligomers, process for their preparation and their use
ES2542501T3 (en) 2005-09-30 2015-08-06 Abbvie Deutschland Gmbh & Co Kg Protein binding domains of the protein family of repulsive targeting molecules (RGM) and functional fragments thereof, as well as their use
KR20180058863A (en) 2005-11-30 2018-06-01 애브비 인코포레이티드 Monoclonal antibodies against amyloid beta protein and uses thereof
RU2442793C2 (en) 2005-11-30 2012-02-20 Эбботт Лэборетриз ANTISUBSTANCES AGAINST GLOBULOMER Aβ, THEIR ANTIGEN-BINDING PARTS, CORRESPONDING HYBRIDOMAS, NUCLEIC ACIDS, VECTORS, HOST CELLS, WAYS OF PRODUCTION OF MENTIONED ANTISUBSTANCES, COMPOSITIONS CONTAINING MENTIONED ANTISUBSTANCES, APPLICATION OF MENTIONED ANTISUBSTANCES AND WAYS OF APPLICATION OF MENTIONED ANTISUBSTANCES
US7846439B2 (en) 2006-02-01 2010-12-07 Cephalon Australia Pty Ltd Domain antibody construct
US8455626B2 (en) 2006-11-30 2013-06-04 Abbott Laboratories Aβ conformer selective anti-aβ globulomer monoclonal antibodies
US8324350B2 (en) 2006-12-29 2012-12-04 Abbott Laboratories Dual-specific IL-1α/IL-1β antibodies
WO2008104386A2 (en) 2007-02-27 2008-09-04 Abbott Gmbh & Co. Kg Method for the treatment of amyloidoses
US8962803B2 (en) 2008-02-29 2015-02-24 AbbVie Deutschland GmbH & Co. KG Antibodies against the RGM A protein and uses thereof
EP3181583A1 (en) 2009-02-24 2017-06-21 ESBATech, an Alcon Biomedical Research Unit LLC Methods for identifying immunobinders of antigens
AU2010262974A1 (en) 2009-06-19 2012-02-02 Medimmune, Llc Protease variants
SG181563A1 (en) 2009-12-08 2012-07-30 Abbott Gmbh & Co Kg Monoclonal antibodies against the rgm a protein for use in the treatment of retinal nerve fiber layer degeneration
ES2684475T3 (en) 2010-04-15 2018-10-03 Abbvie Inc. Proteins that bind to beta amyloid
US9062101B2 (en) 2010-08-14 2015-06-23 AbbVie Deutschland GmbH & Co. KG Amyloid-beta binding proteins
JP2014204669A (en) * 2011-08-18 2014-10-30 公立大学法人名古屋市立大学 Antibody and use thereof
RU2644337C2 (en) 2012-01-27 2018-02-08 Эббви Дойчланд Гмбх Унд Ко. Кг Compositions and methods for diagnostics and treatment of diseases associated with neurites degeneration
CN111999235A (en) * 2020-07-03 2020-11-27 青岛农业大学 Method for rapidly detecting number of viable edible fungus protoplasts by using flow cytometry

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0095361A1 (en) * 1982-05-25 1983-11-30 Eli Lilly And Company Cloning vectors for expression of exogenous protein
WO1994026787A1 (en) * 1993-05-07 1994-11-24 The Board Of Trustees Of The Leland Stanford Junior University Method for generating cell type specific phage antibody libraries

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0095361A1 (en) * 1982-05-25 1983-11-30 Eli Lilly And Company Cloning vectors for expression of exogenous protein
WO1994026787A1 (en) * 1993-05-07 1994-11-24 The Board Of Trustees Of The Leland Stanford Junior University Method for generating cell type specific phage antibody libraries

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FRANCISCO J.A. ET AL.: "Production and fluorescence-activated cell sorting of E. coli expressing a functional antibody fragment on the external surface.", PROC. NATL. ACAD. SCI. USA, vol. 90, November 1993 (1993-11-01), pages 10444 - 0448, XP002084068 *
LITTLE M ET AL: "Human antibody libraries in Escherichia coli", JOURNAL OF BIOTECHNOLOGY, vol. 41, no. 2, 31 July 1995 (1995-07-31), pages 187-195, XP004036935 *

Also Published As

Publication number Publication date
WO1998049286A9 (en) 1999-04-29
AU7171098A (en) 1998-11-24
WO1998049286A2 (en) 1998-11-05

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