WO1998031840B1 - Methods of isolating biological target materials using silica magnetic particles - Google Patents
Methods of isolating biological target materials using silica magnetic particlesInfo
- Publication number
- WO1998031840B1 WO1998031840B1 PCT/US1998/001149 US9801149W WO9831840B1 WO 1998031840 B1 WO1998031840 B1 WO 1998031840B1 US 9801149 W US9801149 W US 9801149W WO 9831840 B1 WO9831840 B1 WO 9831840B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- biological target
- target material
- isolating
- silica magnetic
- complex
- Prior art date
Links
- 239000000463 material Substances 0.000 title claims abstract 38
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 title claims abstract 27
- 239000006249 magnetic particle Substances 0.000 title claims abstract 14
- 239000000377 silicon dioxide Substances 0.000 title claims abstract 13
- 239000002245 particle Substances 0.000 claims abstract 6
- 239000000203 mixture Substances 0.000 claims abstract 5
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 5
- 108020004707 nucleic acids Proteins 0.000 claims abstract 5
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims abstract 4
- 239000000356 contaminant Substances 0.000 claims abstract 2
- 238000010828 elution Methods 0.000 claims abstract 2
- 239000000560 biocompatible material Substances 0.000 claims 2
- 229910052723 transition metal Inorganic materials 0.000 claims 1
- 150000003624 transition metals Chemical class 0.000 claims 1
- 229920000160 (ribonucleotides)n+m Polymers 0.000 abstract 2
- 229920002521 Macromolecule Polymers 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 229910052751 metal Inorganic materials 0.000 abstract 1
- 239000002184 metal Substances 0.000 abstract 1
- 150000002739 metals Chemical class 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Abstract
The present invention provides methods for isolating biological target materials, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from other substances in a medium using silica magnetic particles. The methods of the present invention involve forming a complex of the silica magnetic particles and the biological target material in a mixture of the medium and particles, separating the complex from the mixture using external magnetic force, and eluting the biological target material from the complex. The preferred embodiments of magnetic silica particles used in the methods and kits of the present invention are capable of forming a complex with at least 2 νg of biological target material per milligram of particle, and of releasing at least 60 % of the material from the complex in the elution step of the method. The methods of the present invention produce isolated biological target material which is substantially free of contaminants, such as metals or macromolecular substances, which can interfere with further processing or analysis, if present.
Claims
1. A method for isolating a biological target material from other material in a medium by: a) providing a medium including the biological target material; b) providing silica magnetic particles capable of reversibly binding the biological target material; c) forming a complex of the silica magnetic particles and the biological target material by combining the silica magnetic particles and the medium; d) removing the complex from the medium by application of an external magnetic field; and e) separating the biological target material from the complex by eluting the biological target material, whereby the isolated biological target material is obtained.
2. A method of isolating a biological target material according to claim 1, wherein the biological material isolated according to the method consists of a nucleic acid.
3. A method of isolating a biological target material according to claim 1, wherein the silica magnetic particles provided in step (b) are capable of reversibly binding at least 2 micrograms of biological target material per milligram of particle.
4. A method of isolating a biological target material according to claim 3, wherein the silica magnetic particles provided in step (b) of the method are siliceous- oxide coated magnetic particles.
5. A method of isolating a biological target material according to claim 1, wherein at least 60% of the biological target material in the complex is eluted from the particles in step (e).
6. A method of isolating a biological target material according to claim 1, wherein the biological target material eluted from the complex in step (e) contains no more than 50 parts per million of transition metal contaminants.
7. A method of isolating a biological target material from other materials in a medium comprising the steps of: a) providing a medium containing the biological target material; b) providing a silica magnetic particle with the capacity to reversibly bind at least 2 micrograms of biological target material per milligram of particle; c) forming a mixture comprising the medium and the silica magnetic particle; d) adhering the biological target material to the silica magnetic particle in the mixture; e) removing the silica magnetic particle with the biological target material adhered thereto from the mixture by application of an external magnetic field; and f) eluting at least 60% of the biological target material from the silica magnetic particle by exposing the particle to an elution solution.
8. A method of isolating a biological target material according to claim 7, wherein the biological material isolated according to the method consists of a nucleic acid material.
9. A method of isolating a biological target material according to claim 8, wherein the nucleic acid biological target material isolated according to the method consists of a plasmid DNA material.
10. A method of isolating a biological target material according to claim 8, wherein the nucleic acid biological target material isolated consists of DNA fragment material.
39
STATEMENT UNDER ARTICLE 19
Applicant respectfully submits that none of the amendments to any of the claims presented herein adds any new matter to the subject international application. The amendments are introduced herein to clarify the subject matter of each claim, as filed, and to facilitate examination of the application by the International Search Authority.
Applicant hereby requests communication of the enclosed PCT Article amendments to the Designated Offices, in accordance with PCT Article 20(2).
40
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU66475/98A AU732756B2 (en) | 1997-01-21 | 1998-01-21 | Methods of isolating biological target materials using silica magnetic particles |
JP53467498A JP3253638B2 (en) | 1997-01-21 | 1998-01-21 | Separation method of biological target substance using silica magnetic particles |
DE69817484T DE69817484T2 (en) | 1997-01-21 | 1998-01-21 | METHOD FOR INSULATING SPECIFIC BIOLOGICAL SUBSTANCES BY MEANS OF MAGNETIC SILICA PARTICLES |
EP98908435A EP0895546B1 (en) | 1997-01-21 | 1998-01-21 | Methods of isolating biological target materials using silica magnetic particles |
DE0895546T DE895546T1 (en) | 1997-01-21 | 1998-01-21 | METHOD FOR INSULATING SPECIFIC BIOLOGICAL SUBSTANCES BY MEANS OF MAGNETIC SILICA PARTICLES |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/785,097 US6027945A (en) | 1997-01-21 | 1997-01-21 | Methods of isolating biological target materials using silica magnetic particles |
US08/785,097 | 1997-01-21 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1998031840A1 WO1998031840A1 (en) | 1998-07-23 |
WO1998031840B1 true WO1998031840B1 (en) | 1998-09-17 |
Family
ID=25134438
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/001149 WO1998031840A1 (en) | 1997-01-21 | 1998-01-21 | Methods of isolating biological target materials using silica magnetic particles |
Country Status (9)
Country | Link |
---|---|
US (5) | US6027945A (en) |
EP (2) | EP0895546B1 (en) |
JP (1) | JP3253638B2 (en) |
AU (1) | AU732756B2 (en) |
BR (1) | BR9805897A (en) |
CA (1) | CA2249393A1 (en) |
DE (3) | DE895546T1 (en) |
DK (1) | DK1367137T3 (en) |
WO (1) | WO1998031840A1 (en) |
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US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
CZ297990B6 (en) | 1997-01-21 | 2007-05-16 | W. R. Grace & Co.-Conn. | Particulate adsorbent and process for its preparation |
JP4196227B2 (en) | 1997-05-20 | 2008-12-17 | 東洋紡績株式会社 | Silica particle composition for nucleic acid or protein extraction |
AU3377500A (en) * | 1999-02-26 | 2000-09-14 | Myriad Genetics, Inc. | Method for extracting dna from dried specimens |
-
1997
- 1997-01-21 US US08/785,097 patent/US6027945A/en not_active Expired - Lifetime
-
1998
- 1998-01-21 DE DE0895546T patent/DE895546T1/en active Pending
- 1998-01-21 JP JP53467498A patent/JP3253638B2/en not_active Expired - Fee Related
- 1998-01-21 DK DK03014689.8T patent/DK1367137T3/en active
- 1998-01-21 DE DE69841555T patent/DE69841555D1/en not_active Expired - Lifetime
- 1998-01-21 EP EP98908435A patent/EP0895546B1/en not_active Revoked
- 1998-01-21 CA CA002249393A patent/CA2249393A1/en not_active Abandoned
- 1998-01-21 AU AU66475/98A patent/AU732756B2/en not_active Ceased
- 1998-01-21 EP EP03014689A patent/EP1367137B1/en not_active Revoked
- 1998-01-21 BR BR9805897A patent/BR9805897A/en not_active IP Right Cessation
- 1998-01-21 DE DE69817484T patent/DE69817484T2/en not_active Expired - Lifetime
- 1998-01-21 WO PCT/US1998/001149 patent/WO1998031840A1/en active IP Right Grant
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1999
- 1999-08-20 US US09/377,986 patent/US6673631B1/en not_active Expired - Lifetime
- 1999-12-13 US US09/459,502 patent/US6368800B1/en not_active Expired - Lifetime
-
2002
- 2002-01-07 US US10/041,890 patent/US20020086326A1/en not_active Abandoned
-
2003
- 2003-10-27 US US10/694,475 patent/US20040086930A1/en not_active Abandoned
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