WO1998024928A3 - Detection of chromosomal abnormalities - Google Patents

Detection of chromosomal abnormalities Download PDF

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Publication number
WO1998024928A3
WO1998024928A3 PCT/DK1997/000556 DK9700556W WO9824928A3 WO 1998024928 A3 WO1998024928 A3 WO 1998024928A3 DK 9700556 W DK9700556 W DK 9700556W WO 9824928 A3 WO9824928 A3 WO 9824928A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
mutually distinct
primers
distinct primers
characteristic
Prior art date
Application number
PCT/DK1997/000556
Other languages
French (fr)
Other versions
WO1998024928A2 (en
Inventor
Niels Pallisgaard
Peter Hokland
Original Assignee
Niels Pallisgaard
Peter Hokland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Niels Pallisgaard, Peter Hokland filed Critical Niels Pallisgaard
Priority to EP97946747A priority Critical patent/EP0943013A2/en
Priority to CA002274315A priority patent/CA2274315A1/en
Priority to AU51877/98A priority patent/AU737190B2/en
Priority to JP52509098A priority patent/JP2001505428A/en
Publication of WO1998024928A2 publication Critical patent/WO1998024928A2/en
Publication of WO1998024928A3 publication Critical patent/WO1998024928A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Hospice & Palliative Care (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Testing Or Measuring Of Semiconductors Or The Like (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
  • Oscillators With Electromechanical Resonators (AREA)

Abstract

The present invention has provided a method for detection of the presence or absence of chromosomal abnormalities which are associated with a condition e.g. leukaemia in a subject and are each defined by at least one characteristic nucleic acid sequence. In general, the method comprises subjecting a sample of nucleic acids to a multiplex molecular amplification procedure. The multiplex molecular amplification procedure comprises the use of at least 7 mutually distinct primers in one single reaction mixture, each of the at least 7 mutually distinct primers defining an end of at least one characteristic nucleic acid sequence, and wherein at least one of the at least 7 mutually distinct primers defines the first ends of at least two characteristic nucleic acid sequences, said at least two characteristic nucleic acid sequences each being defined in their opposite ends by mutually distinct primers selected from the remainder of the at least 7 mutually distinct primers, whereby the number of amplified characteristic nucleic acid sequences which can be detected upon conclusion of the amplification reaction is at least 1/2xn+1, wherein n is the number of the at least 7 mutually distinct primers. In one embodiment, the use of an internal positive standard containing: I) a nucleic acid fragment present in the sample, and II) primers for amplification of a nucleotide sequence of said nucleic acid fragment is incorporated into the procedure.
PCT/DK1997/000556 1996-12-06 1997-12-08 Detection of chromosomal abnormalities WO1998024928A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP97946747A EP0943013A2 (en) 1996-12-06 1997-12-08 Detection of chromosomal abnormalities
CA002274315A CA2274315A1 (en) 1996-12-06 1997-12-08 Detection of chromosomal abnormalities
AU51877/98A AU737190B2 (en) 1996-12-06 1997-12-08 Detection of chromosomal abnormalities
JP52509098A JP2001505428A (en) 1996-12-06 1997-12-08 Detection of chromosomal abnormalities

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK1401/96 1996-12-06
DK140196 1996-12-06

Publications (2)

Publication Number Publication Date
WO1998024928A2 WO1998024928A2 (en) 1998-06-11
WO1998024928A3 true WO1998024928A3 (en) 1999-03-25

Family

ID=8104448

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1997/000556 WO1998024928A2 (en) 1996-12-06 1997-12-08 Detection of chromosomal abnormalities

Country Status (5)

Country Link
EP (1) EP0943013A2 (en)
JP (1) JP2001505428A (en)
AU (1) AU737190B2 (en)
CA (1) CA2274315A1 (en)
WO (1) WO1998024928A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105838793A (en) * 2016-04-22 2016-08-10 上海荻硕贝肯生物科技有限公司 Primers, kit and method for qualitatively detecting leukaemia fusion genes

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US7598226B2 (en) 1998-12-28 2009-10-06 Corixa Corporation Compositions and methods for the therapy and diagnosis of breast cancer
US6969518B2 (en) 1998-12-28 2005-11-29 Corixa Corporation Compositions and methods for the therapy and diagnosis of breast cancer
EP1272673B1 (en) * 2000-03-24 2007-03-14 Olympus Corporation Specific multiplex analysis of nucleic acids
ATE353974T1 (en) * 2000-04-03 2007-03-15 Corixa Corp METHODS, COMPOSITIONS AND KITS FOR IDENTIFYING AND MONITORING BREAST CANCER
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
AU2002366179A1 (en) 2001-11-20 2003-06-10 The Regents Of The University Of California Early leukemia diagnostics using microsphere arrays
US7301017B2 (en) * 2002-05-30 2007-11-27 Kolesnick Richard N Kinase suppressor of Ras inactivation for therapy of Ras mediated tumorigenesis
FR2842534B1 (en) * 2002-07-19 2006-01-20 Inst Nat Sante Rech Med GENOME-BASED QUANTITATIVE MULTIPLEX AMPLIFICATION AND APPLICATIONS FOR DETECTION OF GENOMIC REMANIATION
US8323897B2 (en) 2002-12-04 2012-12-04 Applied Biosystems, Llc Multiplex amplification of polynucleotides
KR20220147717A (en) 2013-03-11 2022-11-03 메소 스케일 테크놀러지즈, 엘엘시 Improved methods for conducting multiplexed assays

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Patent Citations (12)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105838793A (en) * 2016-04-22 2016-08-10 上海荻硕贝肯生物科技有限公司 Primers, kit and method for qualitatively detecting leukaemia fusion genes
CN105838793B (en) * 2016-04-22 2019-07-12 上海荻硕贝肯生物科技有限公司 Primer, kit and method for qualitative detection leukemia fusion gene

Also Published As

Publication number Publication date
JP2001505428A (en) 2001-04-24
EP0943013A2 (en) 1999-09-22
AU737190B2 (en) 2001-08-09
AU5187798A (en) 1998-06-29
CA2274315A1 (en) 1998-06-11
WO1998024928A2 (en) 1998-06-11

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