WO1998020139A3 - Phytase from germinated soybeans - Google Patents

Phytase from germinated soybeans Download PDF

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Publication number
WO1998020139A3
WO1998020139A3 PCT/EP1997/006076 EP9706076W WO9820139A3 WO 1998020139 A3 WO1998020139 A3 WO 1998020139A3 EP 9706076 W EP9706076 W EP 9706076W WO 9820139 A3 WO9820139 A3 WO 9820139A3
Authority
WO
WIPO (PCT)
Prior art keywords
phytase
germinated soybeans
soybeans
enzymes
germinated
Prior art date
Application number
PCT/EP1997/006076
Other languages
French (fr)
Other versions
WO1998020139A2 (en
Inventor
Andrew J Morgan
Martin Hessing
Hetty E Sleijster-Selis
Original Assignee
Finnfeeds Int Ltd
Andrew J Morgan
Martin Hessing
Hetty E Sleijster-Selis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Finnfeeds Int Ltd, Andrew J Morgan, Martin Hessing, Hetty E Sleijster-Selis filed Critical Finnfeeds Int Ltd
Priority to BR9712731-0A priority Critical patent/BR9712731A/en
Priority to EP97948873A priority patent/EP0942993A2/en
Priority to CA002269773A priority patent/CA2269773A1/en
Priority to AU70021/98A priority patent/AU7002198A/en
Publication of WO1998020139A2 publication Critical patent/WO1998020139A2/en
Publication of WO1998020139A3 publication Critical patent/WO1998020139A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03026—4-Phytase (3.1.3.26), i.e. 6-phytase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/30—Removing undesirable substances, e.g. bitter substances
    • A23L11/33—Removing undesirable substances, e.g. bitter substances using enzymes; Enzymatic transformation of pulses or legumes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Husbandry (AREA)
  • Agronomy & Crop Science (AREA)
  • Botany (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Fodder In General (AREA)

Abstract

The invention relates to a class of phytate-degrading enzymes which are endogenously present in soya flour, soybeans, germinated soybeans or fractions thereof, to a method for obtaining such enzymes as well as to the use of these enzymes in feed and food applications.
PCT/EP1997/006076 1996-11-05 1997-11-04 Phytase from germinated soybeans WO1998020139A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
BR9712731-0A BR9712731A (en) 1996-11-05 1997-11-04 Phytase from sprouted soy beans
EP97948873A EP0942993A2 (en) 1996-11-05 1997-11-04 Phytase from germinated soybeans
CA002269773A CA2269773A1 (en) 1996-11-05 1997-11-04 Phytase from germinated soybeans
AU70021/98A AU7002198A (en) 1996-11-05 1997-11-04 Phytase from germinated soybeans

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9623133A GB2319030A (en) 1996-11-05 1996-11-05 Phytase extracted from soybean
GB9623133.7 1996-11-05

Publications (2)

Publication Number Publication Date
WO1998020139A2 WO1998020139A2 (en) 1998-05-14
WO1998020139A3 true WO1998020139A3 (en) 1998-07-23

Family

ID=10802550

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1997/006076 WO1998020139A2 (en) 1996-11-05 1997-11-04 Phytase from germinated soybeans

Country Status (7)

Country Link
EP (1) EP0942993A2 (en)
AU (1) AU7002198A (en)
BR (1) BR9712731A (en)
CA (1) CA2269773A1 (en)
GB (1) GB2319030A (en)
PL (1) PL333097A1 (en)
WO (1) WO1998020139A2 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100222638B1 (en) * 1997-01-20 1999-10-01 배희동 Process for producing enzymes using the seeds
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
CN100347303C (en) 1999-03-31 2007-11-07 康乃尔研究基金会有限公司 Phosphatases with improved phytase activity
US6303766B1 (en) 1999-05-14 2001-10-16 Virginia Tech Intellectual Properties, Inc. Soybean phytase and nucleic acid encoding the same
WO2001004147A2 (en) * 1999-07-12 2001-01-18 E.I. Du Pont De Nemours And Company Plant inositol polyphosphate phosphatase homologs
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
EP1749887A3 (en) * 2000-05-04 2007-02-28 Forskningscenter Riso Phytase polypeptides
FR2810994B1 (en) * 2000-06-30 2004-03-12 Biogemma Fr NUCLEIC ACIDS ENCODING A PLANT PHOSPHATASE MIPP TYPE WITH PHYTASIC ACTIVITY AND APPLICATIONS
EP1450627B1 (en) 2001-10-31 2012-09-05 Phytex, Llc Use of phytase containing animal food
CA2925807C (en) 2002-09-13 2020-09-15 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
US20040063184A1 (en) 2002-09-26 2004-04-01 Novozymes North America, Inc. Fermentation processes and compositions
US20040253696A1 (en) 2003-06-10 2004-12-16 Novozymes North America, Inc. Fermentation processes and compositions
US7713562B2 (en) * 2003-09-04 2010-05-11 Rose Acre Farms, Inc. Animal feed and methods for reducing ammonia and phosphorus levels in manure
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
EP2069486A2 (en) 2006-08-03 2009-06-17 Cornell Research Foundation, Inc. Phytases with improved thermal stability
EP3063282B1 (en) 2013-09-11 2020-03-25 Novozymes A/S Processes for producing fermentation products
EP3394275A1 (en) 2015-12-22 2018-10-31 Novozymes A/S Process of extracting oil from thin stillage
EP3682014A1 (en) 2017-09-15 2020-07-22 Novozymes A/S Enzyme blends and processes for improving the nutritional quality of animal feed
EP3701037A1 (en) 2017-10-23 2020-09-02 Novozymes A/S Processes for reducing lactic acid in a biofuel fermentation system
BR112020024347A2 (en) 2018-05-31 2021-02-23 Novozymes A/S processes to increase yeast growth and productivity
BR112021014873A2 (en) 2019-01-31 2021-10-05 Novozymes A/S POLYPEPTIDE, COMBINATION OF ENZYMES, POLYNUCLEOTIDE, NUCLEIC ACID CONSTRUCTION OR RECOMBINANT EXPRESSION VECTOR, RECOMBINANT HOST CELL, METHOD OF PRODUCTION OF A POLYPEPTIDE, AND, PROCESS OF PRODUCTION OF A FERMENTATION PRODUCT
CA3144423A1 (en) 2019-08-05 2021-02-11 Kurt Creamer Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct
WO2021126966A1 (en) 2019-12-16 2021-06-24 Novozymes A/S Processes for producing fermentation products

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0449375A2 (en) * 1990-03-23 1991-10-02 Gist-Brocades N.V. The expression of phytase in plants

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0449375A2 (en) * 1990-03-23 1991-10-02 Gist-Brocades N.V. The expression of phytase in plants

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHRISTEN A. ET AL.: "Cloning of the phytase gene from germinating soybeans", JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 12C, 28 February 1988 (1988-02-28) - 10 April 1988 (1988-04-10), pages 190, XP002062440 *
EHRLICH K.C. ET AL.: "Cloning and sequencing of the phytase gene from soybean", PLANT PHYSIOLOGY, vol. 99, no. 1, SUPPL, May 1992 (1992-05-01), pages 87, XP002030180 *
GIBSON D.M. AND ULLAH A.H.J.: "Purification and Characterization of phytase from cotyledons of germinating soybean seeds", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 260, no. 2, 1 February 1988 (1988-02-01), pages 503 - 513, XP000609747 *
HAMADA J.S.: "Isolation and identification of the multiple forms of soybean phytases", JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY, vol. 73, no. 9, 1996, pages 1143 - 1151, XP002062439 *
SUTARDI AND K.A. BUCKLE: "The characteristics of soybean phytase", JOURNAL OF FOOD BIOCHEMISTRY, vol. 10, no. 1, 1986, pages 197 - 216, XP002062438 *

Also Published As

Publication number Publication date
GB2319030A (en) 1998-05-13
EP0942993A2 (en) 1999-09-22
BR9712731A (en) 1999-10-26
AU7002198A (en) 1998-05-29
GB9623133D0 (en) 1997-01-08
PL333097A1 (en) 1999-11-08
CA2269773A1 (en) 1998-05-14
WO1998020139A2 (en) 1998-05-14

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